3'UTR miRNA Reporters and Cell Lines
abm has developed a comprehensive 3'UTR library of lentiviral vectors and viruses for all human genes, with a choice of luciferase or GFP reporter genes. Also available are ready-to-use 3'UTR reporter cell lines, which stably express the 3'UTR region of your gene of interest and a GFP or luciferase reporter.
One of the most reliable, quantitative methods for studying interactions between a specific miRNA and its putative binding site is by utilization of a reporter gene such as luciferase or GFP cloned upstream of a 3'UTR target. Interaction between an miRNA and the 3'UTR can be validated by observing whether a change in reporter gene expression occurs when miRNA expression vectors and/or miRNA inhibitors are co-transfected with the 3'UTR reporter construct.
- Co-transfect with an miRNA expression vector or mimic. Interaction between the 3'UTR and miRNA is validated if decreased expression of the reporter gene is observed.
- Assay for endogenous miRNA expression by transfecting the 3'UTR reporter vector into the cell line of interest. When co-transfected with a miRNA inhibitor, an attenuated or abolished interaction between the miRNA and the UTR of the Reporter Vector should be observed.
- Investigate miRNA binding requirements using 3'UTR mutant reporters. Importance of a locus for miRNA binding can be determined by mutating that locus and observing if reporter expression is affected.
- Use 3'UTR reporter cell lines for stable expression of the 3'UTR reporter construct from HEK293 cells.
Popular in This Category
Search 3'UTR Reporter Library
3'UTR Reporter Library
We offer human, mouse, and rat 3'UTR reporters in the form of lentivectors, lentiviruses, or stable cells expressing either Luc or GFP.
Isoflurane Increases Neuronal Cell Death Vulnerability by Downregulating miR-214
Yan, H., Xu, T.,et al.
Plos One. 8(2): e55276 (2013).
MIR34A regulates autophagy and apoptosis by targeting HMGB1 in the retinoblastoma cell
Liu, K., et al.
Autophagy 10(3):442-452 (2014).