We offer nearly 18,000 human and 17,500 mouse promoters, available in lentiviral vectors with luciferase or GFP reporters. These vectors can be used directly for plasmid transfection or for packaging into lentivirus to generate stable promoter-reporter cell lines for downstream cell assays.
Use abm’s Promoter-Reporter constructs to:
examine the mechanisms that control gene expression or repression
Our promoter-reporter constructs consist of approximately -1500 bp upstream and +100 bp downstream of the transcription start site, driving the expression of a luciferase or GFP reporter in a lentiviral vector. This can be used directly for transfection or be packaged into virus for infection and Stable Cell Line Generation.
Our custom promoter reporters are PCR amplified from human genome build GRCh38 and mouse genome build GRCm38, and sequences provided upon quotation are a reference only as actual amplified genomic sequences may vary between different sources of template genomic DNA. If exact promoter sequences are required, we recommend ordering custom gene synthesis for the promoters instead.
Xu, Y et al. "Bile acid and cigarette smoke enhance the aggressive phenotype of esophageal adenocarcinoma cells by downregulation of the mitochondrial uncoupling protein-2." Oncotarget 8.60:101057–101071. (2017). DOI: 10.18632/oncotarget.22380 Application: Lentiviral transfection.