BMP Responsive Immortalized Reporter (BRITER) Cell Line

Cat. No.
1x10⁶ cells / 1.0 ml

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Cat. No. T3105
Name BMP Responsive Immortalized Reporter (BRITER) Cell Line

Generated in order to conduct high-thoroughput chemical and molecular genetic screening to identify a novel small molecule alternative activator of BMP signaling. The cells are immortalized tamoxifen inducible Bmp2; Bmp4 double conditional knockout murine (Bmp2c/c;Bmp4c/c; R26CreER neonatal mouse) calvarial osteoblast cells expressing a BMP Response Element(BRE)-dependent Firefly Luciferase (FFLuc) gene and an SV40 promoter/enhancer-dependent Renilla Luciferase (RRLuc) gene. FFLuc activity increases in response to BMP signaling activity while the RRLuc gene acts as an internal control to limit the number of false-positives resulting from non-specific activation of cellular processes, such as cell proliferation. In addition the sensitivity to exogenous BMP signaling can be intensified through the addition of 4-hydroxytamoxifen, which reduces the level of endogenous BMP signaling through the knockout of the Bmp2 and Bmp4 genes resulting in a specific and highly efficient reporter of BRE activation.

Unit 1x10⁶ cells / 1.0 ml
Cell Type Stable Cell Lines
Organism Mouse (M. musculus)
Tissue Bone
Donor History Bmp2c/c;Bmp4c/c; R26CreER neonatal mouse
Growth Properties Adherent, multipolar
Seeding Density (cells/cm2) 10,000
Population Doubling Time (h) 20 - 30
Expression Profile

Osterix, CollA1, BSP, Runx, Osteocalcin, Alkaline phosphatase

Growth Conditions Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow III (TM003) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂
Subculture Protocol

Volumes given below are for a T75 flask; proportionally increase or decrease the volume as required per culture vessel size. Subculture cells once the culture vessel is 80% confluent.

1. Aspirate the culture media, and add 2-3ml of pre-warmed 0.25% Trypsin-EDTA to the culture vessel.

2. Observe the cells under a microscope to confirm detachment (typically within 2-10 minutes). Cells that are difficult to detach can be put in 37°C, for several minutes to facilitate detachment.

3. Neutralize Trypsin-EDTA by adding an equal volume of the complete growth media into the culture vessel.

4. Transfer the culture suspension into a sterile centrifuge tube, and centrifuge at 125xg for 5 minutes. The actual centrifuge duration and speed may vary depending on the cell type.

5. Aspirate the supernatant, and re-suspend the pellet with pre-warmed fresh complete growth media. Add appropriate aliquots of the cell suspension to new culture vessels, as desired.

6. Incubate the cells at the recommended conditions.

Application Research Use Only.
Storage Condition Vapor phase of liquid nitrogen, or below -130°C.
BioSafety II

For Research Use Only


1. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected].

2. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final.

3. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping).

4. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information.

5. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.

6. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."

Depositor Biotech Consortium India Limited
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