Cas9 Proteins
CRISPR is the most versatile technology for genome editing and abm offers the largest selection of Cas9 proteins for CRISPR experiments. Cas9 Ribonucleoproteins (RNPs) are Cas9 proteins in complex with in vitro transcribed sgRNA. These RNPs can be used for in vitro pre-screening sgRNA candidates and can be directly transfected or electroporated into cells to achieve gene editing.
Advantages of Cas9 RNP Delivery Method:
- No transcription or translation required, thus editing is rapid and transient
- No plasmid or virus is used, therefore no risk of genome integration of Cas9/sgRNA machinery
- Proteins are compatible with a wide range of organisms, no need to consider promoters & codon optimization
abm's Cas9 Protein Collection:
| Type | Description | Knowledge Base |
|---|---|---|
| Cas9 Nuclease | High-efficiency CRISPR genome editing nuclease available as spCas9, saCas9 (smaller than spCas9 and suitable for packaging into AAV expression systems) or Cpf1 (targets T-rich genomic sites). | Read more about different Cas9 nucleases here. |
| Cas9 Nickase | Generates single-stranded nicks at target site for repair via the Homology Directed Repair (HDR) pathway to minimize off-target cleavage effects. | Read more about Cas9 nickases here. |
| Cas9 Double Mutant (null mutant, dCas9) | Cas9 with non-functional cleavage domains but with gene targeting function via sgRNA still intact. Useful as a tool for reversible knockdown of gene expression. | Read more about Cas9 null mutants here. |
Additional Information
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