MegaFi™ Pro One-Step RT-PCR

Cat. No.
G597
Unit
100 rxn
Price
$215.00
Cat. No. G597
Name MegaFi™ Pro One-Step RT-PCR
Unit 100 rxn
Category Reverse Transcriptase & RT-PCR
Description

Simple One Step Reaction, Superior Performance!

MegaFi™ Pro One-Step RT-PCR is an enzyme mix of OneScript® Hot Reverse Transcriptase and MegaFi™ Pro Fidelity DNA Polymerase with RNaseOFF Ribonuclease Inhibitor, gel loading dye, and all other necessary reagents in a single One-Step 2X RT-PCR Buffer for both highly sensitive and specific reverse transcription and high fidelity PCR amplification in a single reaction tube from any RNA template. It provides flexibility in choosing desired primers for use with a proprietary RT-PCR buffer containing stabilizers and enhancers that optimize the two reactions in a “single step”. This kit offers simple, efficient reaction setup, and is a reliable alternative to conventional “two-step” sequential RT-PCR.

Product Features:

  • Enjoy simple, one-step RT-PCR reactions
  • 1300X lower error rates due to our MegaFi™ Pro Fidelity DNA Polymerase (Cat. No. G886)
  • Extremely thermostable cDNA synthesis at 50-80°C due to our OneScript® Hot Reverse Transcriptase (Cat. No. G593)
  • Excellent protection against common ribonucleases due to our RNaseOFF Ribonuclease Ihibitor (Cat. No. G591)
  • Earn 2X the rewards points

 

ISO 13485:2016 MDSAP Certified

ISO 13485:2016 MDSAP Certified
Our RT-PCR Products are manufactured under a Quality Management System conforming with ISO 13485:2016 as certified by Intertek (a MDSAP recognized auditing organization).

 


Product Component Quantity
 RT-PCR Enzyme Mix 100 rxn (400 µl)
2X One-Step RT-PCR Buffer 2 x 1.25 ml

Note: This product is abm’s next generation of One-Step RT-PCR Kits and is functionally equivalent to Cat. No. G174 and G174-dye (One-Step RT-PCR Kits), with improved performance. Contact our customer service team [email protected] for more information.


2X the points for PCR Enzymes, Buffers, Solutions, and more!

Buy PCR Get Free Equipment

Storage Condition

Store at -20°C.

Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G597
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Supporting Protocol
There are no FAQs for this product yet!
  • Lee, G et al. "Potential monoclonal antibody therapy for the treatment of ovarian cancer" Ovarian cancer—basic science perspective :385-406 (2012). DOI: 10.5772/1268 . Application: Reverse Transcription.
  • Wu, F., Niu, Z., Zhou, B., Li, P., & Qian, F. "PSMB1 Negatively Regulates the Innate Antiviral Immunity by Facilitating Degradation of IKK-ε" Viruses 11(2):99 (2019). DOI: 10.3390/v11020099.
  • Zhao, G., Zhang, T., Sun, H. J., & Liu, J. "Copper nanoparticles induce zebrafish intestinal defects via endoplasmic reticulum and oxidative stress" Metallomics. : (2019).
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