MycoAway™ Mycoplasma Elimination Cocktail (1000X)
Cat. No.
G398
Unit
1.0 ml
Price
$125.00
Specifications
| Description |
abm’s MycoAway™ Mycoplasma Elimination Cocktail (1000X) is a specially formulated agent to effectively clear cells from Mycoplasma contamination in both adherent and suspension cells. Mycoplasma contamination is currently the most prevalent contamination in all cell culture facilities around the world, damaging precious cell lines by inducing chromosomal aberrations, changes in cell morphology, and cell growth. Our potent formulation is non-toxic and eliminates 70+ species of Mycoplasma in as little as 2-3 weeks (4-6 cell passages)! Pair our MycoAway™ Myoplasma Elimination Cocktail (1000X) with our bestselling PCR Mycoplasma Detection Kit (Cat. No. G238) for complete protection of your cell lines.
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| Cat. No. | G398 | ||
| Name | MycoAway™ Mycoplasma Elimination Cocktail (1000X) | ||
| Category | Mycoplasma Detection & Elimination | ||
| Storage Condition |
Store at -20°C for long term storage. 4°C for short term use. |
Documents
Supporting Protocol
MSDS
STR
There are no STR for this product yet!
FAQs
| What is the basis of the positive control in the mycoplasma kit? | |
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It is DNA fragments of Mycoplasma gDNA diluted in medium.
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| I notice a white precipitate after the delivery of the product; is this normal? | |
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Yes, it is normal to observe a white precipitate in each vial. Please re-suspend the vial to mix the components. The solution is particulate in nature so it is not homologous nor will the particles dissolve. There should be divided solids throughout the tube after mixing. If vortexing doesn't help, you can try pipetting the mixture inside the hood if the pellet is hard to break.
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| Which mycoplasma strains are targeted? | |
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Potential strains which may be affected are:
Mycoplasma pneumonia strains
Mycoplasma hyosynoviae strains
Mycoplasma hyorhinis strains
Mycoplasma synoviae strains
Note: Some strains may naturally become tolerant to this cocktail due to their endogenous defense mechanisms, and thus may show reduced sensitivity. In such cases, other antibiotic combinations may need to be considered.
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| I have used the Mycoplasma Elimination Cocktail on my cells but my cells are dying. Why is this happening? | |
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The mycoplasma cocktail is a very potent combination of different antibiotics to increase the success of eliminating mycoplasma. Even though we have tested and confirmed no cytotoxicity was observed for a variety of cell lines, we would still recommend optimizing the best dilution for your specific cell line in order to achieve best results.
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| There is a ~200-250bp band in one of my samples, does this mean it is mycoplasma positive? | |
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In rare cases, there may be a weak non-specific binding to a region of human gDNA due to the degenerate nature of the primers that can amplify a weak 250bp band in human cell samples. Your sample can be considered as negative for mycoplasma, as mycoplasma amplicons will be 370-550bp in size. You can try to increase the annealing temperature of the PCR program to 60°C to reduce this non-specific amplification.
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References
- Kim, J et al. “1-palmitoyl-2-linoleoyl-3-acetyl-rac-glycerol ameliorates arthritic joints through reducing neutrophil infiltration mediated by IL-6/STAT3 and MIP-2 activation” Oncotarget 8(57):96636-96648 (2017). DOI: 10.18632/oncotarget.19384.
- Badr, C et al. “Intranasal delivery of experimental compounds in orthotopic brain tumor mouse models” STAR protocols 2(1):100290 (2021). DOI: 10.1016/j.xpro.2020.100290.
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