Mycoplasma PCR Detection Kit
Cat. No.
100 rxn

Save 30% when you buy abm's Mycoplasma PCR Detection Kit (Cat. No. G238) with MycoAway™ Elimination Cocktail (1000X) (Cat. No. G398). Simply add both items to your cart and apply promo code: Eliminate at checkout.


Enjoy superior performance at lower costs!

abm's Mycoplasma PCR Detection Kit (Cat. No. G238) offers highly specific and sensitive detection of 200+ strains of Mycoplasmas in less than 2 hours. This bestselling kit is designed to minimize false positives, ensuring quick and reliable routine screening of cell cultures. A recent official publication by a national public health agency found that our kit out-performs Sigma and Lonza's kits while being 6X less expensive than ATCC's kit (Russell et al., 2020).

Mycoplasmas are highly undesirable, easily acquired, and notoriously difficult to detect, altering infected cells at the molecular level and leading to visible changes in cell morphology and growth characteristics. Timely detection of Mycoplasmas in cell cultures is recommended in order to deter wide-spread contamination and save on the costly efforts of elimination. Our proprietary MasterMix formulation contains a gel loading dye, making it convenient for gel electrophoresis.

Kit Features:

  • No DNA isolation needed: Directly add cell culture supernatants to PCR reaction
  • Rapid and Comprehensive: Detect 200+ Mycoplasma species in less than 2 hours
  • Highly Sensitive and Specific: Demonstrated the highest true positive and lowest false positive rates compared to competing kits
  • Easy-to-use: Includes ready-to-use primer mix and positive control
  • 6X less expensive per sample compared to competing kits (Russel et al., 2020)
  • Out-performs Sigma and Lonza's kits (Russel et al., 2020)
  • Combine with our bestselling Mycoplasma Elimination Cocktail (Cat. No. G398) to eliminate 70+ species of Mycoplasma

Request Free Sample


Customer Testimonial

"The mycoplasma detection kit we received from ABM worked very well. It was fast, easy to use, and, with the included controls, the results were very easy to interpret. We will purchase this kit again the next time we are in need of mycoplasma detection."

- Haylie Romero, University of California San Diego


An official publication by a national public health agency finds our kit out-performs Sigma and Lonza's kits:

Russell, B. J., Horiuchi, K., Velez, J. O., Goodman, C. H., & Johnson, B. W. (2020). Mycoplasma detection in a historical arbovirus repository: Commercial kit comparison and implications for improved repository management. Journal of virological methods, 276, 113769.


ISO 13485:2016 MDSAP Certified

ISO 13485:2016 MDSAP Certified
Our PCR Products are manufactured under a Quality Management System conforming with ISO 13485:2016 as certified by Intertek (a MDSAP recognized auditing organization).


Product Components Quantity
BlasTaq™ 2X PCR MasterMix
1.25 ml
Primer Mix
100 μl
Positive Control
250 μl
Nuclease-Free H2O
1.0 ml

Why test your cells for Mycoplasma?

Due to its small size, Mycoplasma can be present in your growth media without being visibly detected. This can cause significant errors in your experiments due to its effects on:

  • proliferation
  • metabolism
  • gene synthesis & processing
  • adhesion properties
  • and more

Many publishers now request Mycoplasma testing to be reported along with manuscript submissions to ensure accuracy in data reporting. Visit our learning resources center to read more about Mycoplasma contamination.

2X the points for PCR Enzymes, Buffers, Solutions, and more!

Buy PCR Get Free Equipment



Cat. No. G238
Name Mycoplasma PCR Detection Kit
Unit 100 rxn
Category Mycoplasma Detection & Elimination
Storage Condition

Store at -20°C. This product is stable for 2 years from the date of shipping if stored and handled properly. 

There are no STR for this product yet!
  • Koh, JH et al. "Effect of water-soluble fraction of cherry tomatoes on the adhesion of probiotics and Salmonella to intestinal epithelial cells" J Sci Food Agric : (2013). PubMed: 23749725. Application: Cell Culture.
  • Jagadish, N et al. "A-kinase anchor protein 4 (AKAP4) a promising therapeutic target of colorectal cancer" J Exp Clin Cancer Res 34(1):142 (2015). DOI: 10.1186/s13046-015-0258-y. PubMed: 26590805. Application: Cell Culture.
  • Saha, A et al. "Effect of Metformin, Rapamycin, and Their Combination on Growth and Progression of Prostate Tumors in HiMyc Mice." Cancer Prev Res (Phila) 8(7):597-606 (2015). DOI: 10.1158/1940-6207.CAPR-15-0014. PubMed: 25908508. Application: Cell Culture.
  • Jagadish, N et al. "Heat shock protein 70–2 (HSP70-2) is a novel therapeutic target for colorectal cancer and is associated with tumor growth" BMC Cancer 1:561 (2016). DOI: 10.1186/s12885-016-2592-7. Application: Mycoplasma detection.
  • Blouin, M. J., Parisotto, M., Papavasiliou, V., Lavoie, C., Larsson, O., Ohh, M., ... & Ferbeyre, G. "Translational and HIF-1a-Dependent Metabolic Reprogramming Underpin Metabolic Plasticity and Responses to Kinase Inhibitors and Biguanides" Cell Metabolism 28:1-16 (2018).
  • Bufalieri, F., Infante, P., Bernardi, F., Caimano, M., Romania, P., Moretti, M., … Di Marcotullio, L. "ERAP1 promotes Hedgehog-dependent tumorigenesis by controlling USP47-mediated degradation of βTrCP" Nature Communications 10(1): (2019). DOI: 10.1038/s41467-019-11093-0.
  • Carter, B. Z., Mak, P. Y., Wang, X., Tao, W., Ruvolo, V., Mak, D., … Andreeff, M. "An ARC-Regulated IL1β/Cox-2/PGE2/β-Catenin/ARC Circuit Controls Leukemia–Microenvironment Interactions and Confers Drug Resistance in AML" Cancer Research 79(6):1165–1177 (2019). DOI: 10.1158/0008-5472.can-18-0921.
  • Chakravorty, S., Yan, B., Wang, C., Wang, L., Quaid, J. T., Lin, C. F., ... & Chopra, G. "Integrated Pan-Cancer Map of EBV-Associated Neoplasms Reveals Functional Host–Virus Interactions"  Cancer research. : (2019).
  • Guarino, A.M., Mauro, G.D., Ruggiero, G. "doi:10" 1038/s41598-019-45468-6 : (2019). DOI: 10.1038/s41598-019-45468-6.
  • Hsu, H. P., Wang, C. Y., Hsieh, P. Y., Fang, J. H., & Chen, Y. L. "Knockdown of serine/threonine-protein kinase 24 promotes tumorigenesis and myeloid-derived suppressor cell expansion in an orthotopic immunocompetent gastric cancer animal model" Journal of Cancer 11(1):213-228 (2020).
  • Hsueh, Y. S., Chang, H. H., Shan, Y. S., Sun, H. S., Fletcher, J. A., Li, C. F., & Chen, L. T. "Nuclear KIT induces a NFKBIB-RELA-KIT autoregulatory loop in imatinib-resistant gastrointestinal stromal tumors" Oncogene 38(38):6550-6565 (2019).
  • Ide, H., Inoue, S., Mizushima, T., Kashiwagi, E., Zheng, Y., & Miyamoto, H. "Role of glucocorticoid signaling in urothelial tumorigenesis: Inhibition by prednisone presumably through inducing glucocorticoid receptor transrepression" Molecular carcinogenesis. : (2019).
  • Inoue, S., Ide, H., Mizushima, T., Jiang, G., Netto, G. J., Gotoh, M., & Miyamoto, H. "Nuclear Factor-κB Promotes Urothelial Tumorigenesis and Cancer Progression via Cooperation with Androgen Receptor Signaling" Molecular Cancer Therapeutics 17(6):1303–1314 (2018). DOI: 10.1158/1535-7163.mct-17-0786.
  • Inoue, S., Mizushima, T., Ide, H., Jiang, G., Goto, T., Nagata, Y., … Miyamoto, H. "ATF2 promotes urothelial cancer outgrowth via cooperation with androgen receptor signaling" Endocrine Connections 1397–1408: (2018). DOI: 10.1530/ec-18-0364.
  • Jones, P. S., Yekula, A., Lansbury, E., Small, J. L., Ayinon, C., Mordecai, S., ... & Ghiran, I. "Characterization of plasma-derived protoporphyrin-IX-positive extracellular vesicles following 5-ALA use in patients with malignant glioma" EBioMedicine. : (2019).
  • Kollara, A., Shathasivam, P., Park, S., Ringuette, M. J., & Brown, T. J. "Increased Androgen Receptor Levels and Signaling in Ovarian Cancer Cells by VEPH1 Associated with Suppression of SMAD3 and AKT Activation" The Journal of Steroid Biochemistry and Molecular Biology 105498: (2019).
  • Lee, R. S., Zhang, L., Berger, A., Lawrence, M. G., Song, J., Niranjan, B., ... & Risbridger, G. P. "Characterization of the ERG-regulated Kinome in Prostate Cancer Identifies TNIK as a Potential Therapeutic Target" Neoplasia 21(4):389-400 (2019).
  • Linley, A. J., Griffin, R., Cicconi, S., D'Avola, A., MacEwan, D. J., Pettitt, A. R., ... & Slupsky, J. "Kinobead profiling reveals reprogramming of B-cell receptor signaling in response to therapy within primary CLL cells" bioRxiv 841312: (2019).
  • Lospinoso Severini, L., Quaglio, D., Basili, I., Ghirga, F., Bufalieri, F., Caimano, M., ... & Maroder, M. "A Smo/Gli Multitarget Hedgehog Pathway Inhibitor Impairs Tumor Growth" Cancers 11(10):1518 (2019).
  • Mehrpour, M., Rodriguez, R., Hamai, A., & Trang, M. "U" S. Patent Application No. 16/082 970: (2019).
  • Nii, T., Prabhu, V.V, Ruvolo, V., Madhukar, N., Zhao, R., Mu, H., Heese, L., Nishida, Y., Kojima, K., Garnett, M.J., McDermott, U., Benes, C.H., Charter, N., Deacon, S., Elemento, O., Allen, J.E., Oster, W., Stogniew, M., Ishizawa, J., Andreeff, M. "Imipridone ONC212 activates orphan G protein-coupled receptor GPR132 and integrated stress response in acute myeloid leukemia" Leukemia. : (2019). DOI: 10.1038/s41375-019-0491-z.
  • Ong, F., Dreesen, O., & Dröge, P. . "The RNA interactome of human telomerase RNA reveals a coding-independent role for 2 a histone mRNA in telomere homeostasis 3" : ().
  • Rosenberger, L., Ezquer, M., Lillo-Vera, F., Pedraza, P. L., Ortúzar, M. I., González, P. L., … Alcayaga-Miranda, F. "Stem cell exosomes inhibit angiogenesis and tumor growth of oral squamous cell carcinoma" Scientific Reports 9(1): (2019). DOI: 10.1038/s41598-018-36855-6.
  • Ross, J., Bressler, K., & Thakor, N. "Eukaryotic Initiation Factor 5B (eIF5B) Cooperates with eIF1A and eIF5 to Facilitate uORF2-Mediated Repression of ATF4 Translation" International Journal of Molecular Sciences 19(12):4032 (2018). DOI: 10.3390/ijms19124032.
  • Salvati, A., Gigantino, V., Nassa, G., Giurato, G., Alexandrova, E., Rizzo, F., … Weisz, A. "The Histone Methyltransferase DOT1L Is a Functional Component of Estrogen Receptor Alpha Signaling in Ovarian Cancer Cells" Cancers 11(11):1720 (2019). DOI: 10.3390/cancers11111720.
  • Severini, L. L., Quaglio, D., Basili, I., Ghirga, F., Bufalieri, F., Caimano, M., ... & Maroder, M. "A Smo/Gli Multitarget Hedgehog Pathway Inhibitor Impairs Tumor Growth" Cancers 11(10): (2019).
  • Shishodia, G., Koul, S., & Koul, H. K. "Protocadherin 7 is overexpressed in castration resistant prostate cancer and promotes aberrant MEK and AKT signaling" The Prostate 79(15):1739–1751 (2019). DOI: 10.1002/pros.23898.
  • Singh, S., Kumar, M., Kumar, S., Sen, S., Upadhyay, P., Bhattacharjee, S., ... & Kundu, T. K. "The cancer-associated, gain-of-function TP53 variant P152Lp53 activates multiple signaling pathways implicated in tumorigenesis" Journal of Biological Chemistry 294(38):14081-14095 (2019).
  • Spiombi, E., Angrisani, A., Fonte, S., De Feudis, G., Fabretti, F., Cucchi, D., ... & Di Magno, L. "KCTD15 inhibits the Hedgehog pathway in Medulloblastoma cells by increasing protein levels of the oncosuppressor KCASH2" Oncogenesis 8(11):1-11 (2019).
  • Wang, D., Luo, H., Huo, Z., Chen, M., Han, Z., Hung, M., ... & Xiao, H. "Irradiation-induced dynamic changes of gene signatures reveal gain of metastatic ability in nasopharyngeal carcinoma" American Journal of Cancer Research 9(3):479 (2019).
  • Wen, M. H., Xie, X., Tub, J., Lee, D. F., & Chen, T. Y. "Generation of a genetically modified human embryonic stem cells expressing fluorescence tagged ATOX1" Stem Cell Research 101631: (2019).
  • Wilson, M. R., Reske, J. J., Holladay, J., Wilber, G. E., Rhodes, M., Koeman, J., ... & Patterson, A. L. "ARID1A and PI3-kinase pathway mutations in the endometrium drive epithelial transdifferentiation and collective invasion" Nature communications 10(1):1-18 (2019).
  • Yekula, A., Minciacchi, V. R., Morello, M., Shao, H., Park, Y., Zhang, X., ... & Carter, B. "Large and small extracellular vesicles released by glioma cells in vitro and in vivo" Journal of Extracellular Vesicles 9(1):1689784 (2019).
  • Russell, B. J., Horiuchi, K., Velez, J. O., Goodman, C. H., & Johnson, B. W. (2020). Mycoplasma detection in a historical arbovirus repository: Commercial kit comparison and implications for improved repository management. Journal of virological methods, 276, 113769.
Controls and Related Product:

1 Sample

1 Sample


1 Box
Other Cell Lines
Select Species:
African Green Monkey (C. aethiops) (0)
Bat (0)
Bee (0)
Bottlenose Dolphin (Tursiops) (0)
Cabbage Looper (T. ni) (0)
Cat (Feline) (0)
Cattle (0)
Chicken (Galline) (0)
Chinese Hamster (C. griseus) (0)
Cow (Bovine) (0)
Dog (Canine) (0)
Duck (Anas) (0)
Duck (Anatidae) (0)
E. coli (0)
European Sea Bass (0)
European Sea Bass (D. labrax) (0)
Fall Armyworm (S. frugiperda) (0)
Fish (0)
Fly (D. melanogaster) (0)
Frog (Xenopus) (0)
Fruit Fly (D. melanogaster) (0)
Gilthead Sea Bream (S. aurata) (0)
Goat (Capra) (0)
Golden Hamster (M. auratus) (0)
Goose (Anatidae) (0)
Green Monkey (C. aethiops) (0)
Green Monkey (C. sabaeus) (0)
Hamster (M. auratus) (0)
Horse (Equine) (0)
Human (H. sapiens) (0)
Insect (0)
Killifish (F. heteroclitus) (0)
Luciola italica (0)
Marmoset (Primate) (0)
Mink (0)
Monkey (Primate) (0)
Moth (S. frugiperda) (0)
Mouse (M. musculus) (0)
Other (0)
Photinus pyralis (0)
Pig (Porcine) (0)
Quail (Coturnix) (0)
Rabbit (Leporidae) (0)
Rabbit (Leporine) (0)
Rainbow Trout (O. mykiss) (0)
Rat (R. norvegicus) (0)
Rat (Rattus) (0)
Renilla reniformis (0)
Roundworm (C. elegans) (0)
Rous Sarcoma Virus (RSV) (0)
SARS-CoV-2 (0)
Sheep (Ovine) (0)
Sheep (Ovis) (0)
Turtle (0)
Universal (0)
Zebrafish (D. rerio) (0)
Select Tissue:
0 (0)
Achilles Tendon (0)
Adipose (0)
Adrenal Gland (0)
Adrenal Glands (0)
Airway/Lung (0)
Amniotic Sac (0)
Antimesometrial Decidua (0)
antler (0)
Ascites (0)
Auditory (0)
Bee Cell (0)
Bile Duct (0)
Bladder (0)
Blood (0)
Blood Vessel (0)
Bone (0)
Bone Marrow (0)
Brain (0)
Breast (0)
Caput Epididymis (0)
Cartilage (0)
Cerebrospinal Fluid (0)
Cervix (0)
Colon (0)
Connective Tissue (0)
Cord Blood (0)
Digestive (0)
Digestive System (0)
Embryo (0)
embryonic (0)
Endometria (0)
Endometrium (0)
Esophagus (0)
Eye (0)
Fibroblast (0)
Gingiva (0)
Hair (0)
Hair Follicle (0)
Heart (0)
HeLa (0)
Hepatocellular Carcinoma (0)
Hippocampus (0)
Intestinal (0)
Intestine (0)
Kidney (0)
Kidney, Cortex/Proximal tubule (0)
Labial Salivary Gland (0)
Larynx (0)
Liver (0)
Lung (0)
Lung Carcinoma (0)
Lung, bronchus (0)
Lymph node (0)
Lymphatic System (0)
lymphoblast (0)
Malignant melanoma (0)
Mammary (0)
Mesencephalic Tissue (0)
Mesentery (0)
Microglia (0)
Muscle (0)
Myoblast (0)
Nasal Mucosa (0)
Nerve (0)
Nervous System (0)
Oesophageal (0)
Oesophagus (0)
Oral (0)
Organ of Corti (0)
Other (0)
Ovary (0)
Pancreas (0)
Pancreatic (0)
Parathyroid (0)
Peripheral Blood (0)
Peripheral Blood Mononuclear Cells (0)
Peritoneal (0)
Peritoneum (0)
Pituitary (0)
Pituitary Gland (0)
Pituitary Tumor (0)
Placenta (0)
promyelocytic leukemia (0)
Prostate (0)
Prostate carcinoma (0)
Renal (0)
Reproductive (0)
Reproductive System (0)
Retina (0)
Salivary Gland (0)
Sciatic Nerve (0)
Skeletal (0)
Skeletal Muscle (0)
Skin (0)
Skin ; Keratinocytes (0)
Small intestine (0)
Soft Tissue (0)
Spleen (0)
Stem Cell (0)
Stomach (0)
Straitum (0)
Sweat Gland (0)
Testis (0)
Thymic (0)
Thymus (0)
Thyroid (0)
Tongue (0)
Tonsil (0)
Trachea (0)
Tumor (0)
Unknown (0)
Uterus (0)
Uterus; endometrium (0)
Vascular Endothelium (0)
Ventral Mesencephalon (0)
Vertebral Disc (0)
// //