saCas9 Nuclease Protein (High Concentration)

Cat. No.

K144

Unit

32.5µg (250pmol/ 25µL)

Price

$155.00

Cat. No.	K144
Name	saCas9 Nuclease Protein (High Concentration)
Unit	32.5µg (250pmol/ 25µL)
Category	Molecular Biology Enzymes and Kits
Description	The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided endonuclease tool in genome editing which allows for very specific genomic disruption and replacement. The Cas9 nuclease serves to unwind the genomic DNA duplex next to conserved protospacer adjacent motifs (PAMs) and homes in on its target sequence, which is recognized by a complementary single-guide RNA. The resulting double-stranded break gets repaired by the non-homologous end joining (NHEJ) pathway, leading to a disruption in the open reading frame of the targeted gene. Alternatively, by supplying a suitable repair template, virtually any desired point mutation can be introduced at the break point via homology-directed repair (HDR). The Cas9 nuclease from the bacteria Staphylococcus aureus, abbreviated saCas9, is gaining popularity as an alternative to spCas9 due to its relatively smaller size. The saCas9 PAM sequence is 5’-NNGRRN (preferably 5’-NNGRRT).
Cas Type	Nuclease
Cas Origin	saCas9
Cas Protein Marker	No GFP
Concentration	10 µM, 1.30 mg/ml
Format General	Enzyme supplied with 10X Reaction Buffer
Endotoxin Level	<1.0 EU/μg of recombinant protein as determined by the LAL method.
Storage Buffer	10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM DTT, 300 mM NaCl, and 50% (v/v) Glycerol.
Storage Condition	Store all components at -20°C.
Caution	This product is distributed for laboratory research only. Not for diagnostic use.

Datasheet

saCas9 Nuclease Protein Datasheet

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MSDS

	Does K144 contain a HIS-tag?
	Yes, it does contain a His-tag.

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