|The most advanced RNA tracking, visualization and pull down technology.
Technology for studying the diverse cellular roles of RNA has lagged behind the tools for studying DNA and proteins, but innovative researchers are working to change that! One such researcher is Dr. Peter Unrau of Simon Fraser University. He and his team have created RNA Mango, a novel technology with a number of useful applications.
RNA Mango technology is based on the specific binding of the RNA Mango Aptamer and a Thizole Orange (TO) bi-functional dye. The main features of this technology is the tight binding between the dye and aptamer (KD ≈ 3nM) , and the strong ~1000X enhancement of the dye’s fluorescence when bound to the Mango aptamer (Fluorescent enhancement FE=1,100).
The Thizole Orange (TO) dye has a number of other desirable properties including:
- Small size
- Lack of toxicity
- Plasma and nuclear membrane permeability
- Short intracellular half-life
- The accessibility of a broad wavelength range simply via substitutions and alterations to the TO structure
- The TO1 and TO3 dyes can be used in a 2-color reporter assay system using both the RNA Mango and RNA Peach aptamer systems (Kong et. al. 2021)
TO1-biotin is the standard variety of TO dye for RNA Mango experiments. View our complete list of RNA Mango dyes.
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The RNA Mango Workflow
|TO1-3PEG-Biotin Fluorophore||0.5 mg/ml (500 µl)||G955|
|TO1-3PEG-Desthiobiotin Fluorophore||0.5 mg/ml (500 µl)||G956|
|TO3-3PEG-Biotin Fluorophore||0.5 mg/ml (500 µl)||G959|
|YO3-3PEG-Biotin Fluorophore||0.5 mg/ml (500 µl)||G957|
Ribonucleoprotein purification and characterization using RNA Mango.
Panchapakesan SSS, Ferguson ML, Hayden EJ, Chen X, Hoskins AA, Unrau PJ. et al.
RNA. 2017 Oct;23(10):1592-1599.
DOI: 10.1261/rna.062166.117. PubMed: 28747322. PubMed Central PMCID: PMC5602116.
Structural basis for high-affinity fluorophore binding and activation by RNA Mango.
Trachman RJ 3rd, Demeshkina NA, Lau MWL, Panchapakesan SSS, Jeng SCY, Unrau PJ, Ferré-D'Amaré AR. et al.
Nat Chem Biol. 2017 Jul;13(7):807-813.
DOI: 10.1038/nchembio.2392. Epub 2017 May 29. PubMed : 28553947.PubMed Central PMCID: PMC5550021.
Fluorophore ligand binding and complex stabilization of the RNA Mango and RNA Spinach aptamers.
Jeng SC, Chan HH, Booy EP, McKenna SA, Unrau PJ. et al
RNA. 2016 Dec;22(12):1884-1892. Epub 2016 Oct 24
PubMed : 27777365. PubMed Central PMCID: PMC5113208.