Kodaq (KOD) 2X PCR MasterMix
| Cat. No. | G883 |
| Name | Kodaq (KOD) 2X PCR MasterMix |
| Unit | 800 reactions (10.0 ml) |
| Category | PCR Polymerase |
| Description |
Kodaq (KOD enzyme) 2X PCR MasterMix is a ready-to-use mixture containing all the necessary reagents for amplification of DNA in a uniquely-formulated buffer with gel loading dye. This master mix contains Kodaq DNA Polymerase (KOD enzyme) which is a DNA polymerase with strategically engineered mutations resulting in a robust and a high fidelity polymerase. Kodaq DNA polymerase has exceptional 3’ to 5’ exonuclease activity that endows it with superior accuracy over competitor polymerases. This enzyme intrinsically has high processivity and is engineered to have an improved binding affinity for DNA resulting in a highly successful PCR. Furthermore, the uniquely-formulated buffer tolerates high AT and GC content, as well as many PCR inhibitors commonly found in a typical DNA sample. |
| Note |
Suitable for long range PCR up to 18 kb from less difficult targets or up to 15 kb from genomic DNA. |
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G883 |
| I am observing positive results in my negative controls (no template added) when attempting to detect gene sequences in a bacterial strain. I suspect this may be due to residual bacterial DNA from the DNA polymerase. What steps should I take to address this issue? | |
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The presence of residual bacterial DNA is quite common in commercially available DNA polymerases, as most are expressed and purified using E. coli recombinant systems. In this case, we recommend switching to our Ultra-Pure BlasTaq™ 2X PCR MasterMix (Cat. No. G885). This product undergoes a rigorous multi-step purification protocol utilizing physical, chemical, and enzymatic methods to maximize the removal of contaminating genomic DNA.
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