Anti-Histone H2A.X (Phospho-Ser139) Antibody
Cat. No.
Y011268
Unit
100 μg
Price
$319.00
| Cat. No. | Y011268 |
| Name | Anti-Histone H2A.X (Phospho-Ser139) Antibody |
| Clone | N/A |
| Category | Polyclonal Antibodies |
| Gene Name | Histone H2A-X |
| Application | WB |
| Application Info | WB: 1:500~1:1000 |
| Raised In | Rabbit |
| Clonality | Polyclonal |
| Reactivity | Human,Mouse,Rat |
| Immunogen | The antiserum was produced against synthesized phosphopeptide derived from human Histone H2A.X around the phosphorylation site of serine 139 (Q-A-SP-Q-E). |
| Concentration | 1ug/ul |
| Recommended Dilutions | WB: 1:500~1:1000 |
| Storage Buffer | phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage Condition | This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt. |
| Formulation | Liquid |
| Species Reactivity | Human,Mouse,Rat |
| Specificity | Histone H2A.X (phospho-Ser139) antibody detects endogenous levels of Histone H2A.X only when phosphorylated at serine 139. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. |
| Guarantee | abm guarantees that all our Anti-Histone H2A.X (Phospho-Ser139) Antibody will perform as described on this product webpage, if this is not the case we will provide you with a one-time replacement at no extra cost. Documentation and explanation of experiment conducted will be required when submitting a claim for replacement. |
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Y011268 |
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| I see two bands on my western blot. One is 50kda, and one is 20kda. Is this normal? | |
|
The 20kda is the protein of interest, the histone H2A.X. This antibody usually produce a 50kd non-specific band in western blot, but this do not affect its detection and specificity.
|
| My 20kDa band is weak? How do I resolve this? | |
|
Y011268 is a phospho-antibody and the signal strength is dependent on how many of the H2A.X proteins are phosphorylated at the Serine 139 site. Many methods can stimulate the phosphorylation of this site, such as UV and ionizing radiation. Some methods that can stimulate MAPK / JNK pathway can also stimulate this site. It is important to maintain a strong level of phosphorlyation for the protein when producing cell lysate. We suggest optimizing the stimulating method for the sample to increase the detection from the antibody.
|
- Li, A et al. "Phosphorylation of histone H2A.X by DNA-dependent protein kinase is not affected by core histone acetylation, but it alters nucleosome stability and histone H1 binding" J. Biol. Chem. 285:17778-88 (2010). PubMed: 20356835. Application: Western Blot.
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