Anti-Human IL-10 Antibody
Cat. No.
Y050209
Unit
100 μl
Price
$839.00
| Cat. No. | Y050209 |
| Name | Anti-Human IL-10 Antibody |
| Clone | Polyclonal |
| Category | Polyclonal Antibodies |
| Gene Name | Human IL-10 |
| Application | WB,IHC,ELISA,IP,Other |
| Application Info | This antiserum has been tested for use in neutralizations, ELISA, immunohistochemistry, radioimmunoassay, immunoprecipitation, immunohistochemistry and immunoblotting. The antibody recognizes hrIL-10. Reactivity in other immunoassays is unknown. |
| Raised In | Rabbit |
| Clonality | Polyclonal |
| Reactivity | Human |
| Immunogen | The whole rabbit serum was prepared by repeated immunizations with recombinant human IL-10 produced in E.coli. |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Recommended Dilutions | This antiserum has been tested for use in neutralizations, ELISA, immunohistochemistry, radioimmunoassay, immunoprecipitation, immunohistochemistry and immunoblotting. The antibody recognizes hrIL-10. Reactivity in other immunoassays is unknown. |
| Storage Buffer | PBS, pH 7.4 with 0.05% sodium azide. |
| Storage Condition | This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt. |
| Formulation | Liquid |
| Species Reactivity | Human |
| Specificity | This antiserum has been heated to 56°C for 30 minutes. The antiserum detects recombinant and native IL-10 present in body fluids and cell supernatants in various assays (ie. IL-1 stimulated IL- 10 production from fibroblasts). In Western blot analysis of natural cell products_recover or human body fluids, multiple bands of IL-10 will appear due to the variable amount of glycosylation on the molecule. The antiserum is also useful for neutralization of human of IL-10 activity in bioassays. For neutralization, incubate the sample with a 1:400 dilution of the antiserum for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG (heat inactivated) is recommended. The antibody will not neutralize the biological activity of murine IL-10. |
| Purity | Purified |
| Guarantee | abm guarantees that all our Anti-Human IL-10 Antibody will perform as described on this product webpage, if this is not the case we will provide you with a one-time replacement at no extra cost. Documentation and explanation of experiment conducted will be required when submitting a claim for replacement. |
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Y050209 |
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