Anti-Mouse TNF alpha Antibody
Cat. No.
Y050225
Unit
100 μl
Price
$839.00
| Cat. No. | Y050225 |
| Name | Anti-Mouse TNF alpha Antibody |
| Clone | Polyclonal |
| Category | Polyclonal Antibodies |
| Gene Name | Mouse TNF α |
| Application | WB,IHC,ELISA |
| Application Info | This antiserum against Mouse TNFα has been tested for use in neutralizations, ELISA, immunohistochemistry, radioimmunoassay, immunoprecipitation, immunohistochemistry and immunoblotting. It recognizes the 17,000 MW TNFα. Reactivity in other immunoassays is unknown. |
| Raised In | Rabbit |
| Clonality | Polyclonal |
| Reactivity | Mouse |
| Immunogen | The whole rabbit serum was prepared by repeated immunizations with recombinant mouse TNFα produced in E.coli. |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Recommended Dilutions | This antiserum against Mouse TNFα has been tested for use in neutralizations, ELISA, immunohistochemistry, radioimmunoassay, immunoprecipitation, immunohistochemistry and immunoblotting. It recognizes the 17,000 MW TNFα. Reactivity in other immunoassays is unknown. |
| Storage Buffer | PBS, pH 7.4 with 0.05% sodium azide. |
| Storage Condition | This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt. |
| Formulation | Liquid |
| Species Reactivity | Mouse |
| Specificity | This antiserum has been heated to 56°C for 30 minutes. The antiserum is directed against mature 17,000 MW mouse TNFα and is useful in determining its presence in various assays. The antibody does not recognize mouse TNFβ (lymphotoxin). This antiserum will recognize the cell-bound precursor of TNFα as a 26,000 protein in immunoblots, particularly in denatured samples. This antibody is also useful for neutralization of mouse activity in bioassays. It does not neutralize the biological activity of lymphotoxin. For neutralization, it is recommended to incubate the sample with a 1:200 dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended. |
| Purity | Purified |
| Guarantee | abm guarantees that all our Anti-Mouse TNF alpha Antibody will perform as described on this product webpage, if this is not the case we will provide you with a one-time replacement at no extra cost. Documentation and explanation of experiment conducted will be required when submitting a claim for replacement. |
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Y050225 |
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