Anti-PKA-RIB (Protein Kinase-A Regulatory Subunit Ib) Antibody
Cat. No.
Y051648
Unit
100μg
Price
$978.00
| Cat. No. | Y051648 |
| Name | Anti-PKA-RIB (Protein Kinase-A Regulatory Subunit Ib) Antibody |
| Clone | Polyclonal |
| Category | Polyclonal Antibodies |
| Gene Name | Pka |
| Application | WB |
| Application Info | Recommended dilutions WB: 1/1,000 when using colorimetric substrates such as BCIP/NBT and 1/5,000 for chemiluminescent substrates. Higher concentration of antibody may be needed for non-human samples. Predicted molecular weight: 43 kDa. This antibody identifies bands at 55 kD and 53 kD when used against the reduced protein as well as activation/breakdown products_recover. The antibody can recognize native or reduced PKARIb. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Raised In | Rabbit |
| Clonality | Polyclonal |
| Reactivity | Human,Mouse,Rat,Other |
| Description | The antibody is a rabbit antibody to the regulatory subunit Ib of protein kinase-A, made with a synthetic peptide based on the rat sequence. The antibody binds to PKARIb, but does not cross react with the other PKA regulatory subunits (PKARIa, PKARIIa, PKARIIb). The antibody has been peptide-affinity purified, concentrated to 1 mg/ml, with the addition of Sodium Azide as preservative, and 50% glycerol as as cryoprotectant. Protein KinaseA is a critical kinase involved in numerous biological functions. The regulatory subunits of PKA allow a range of modifying agents to influence PKA activity and localization, and are found in different levels in a tissue-specific manner. RP1PKARIb works well for Western Blotting applications, and when used against the reduced protein identifies bands at 55 kD and 53 kD, as well as activation/breakdown products_recover. The antibody can recognize native or reduced PKARIb, and has application for immunoprecipitation, immunohistochemistry, cell sorting and ELISA. RP1PKARIb recognized human, rat, mouse and rabbit PKARIb. A recommended starting concentration for Western blots is 1:1,000 when using colorimetric substrates such as BCIP/NBT, and 1:5,000 for chemiluminescent substrates. Higher concentration of antibody may be needed for non-human samples. FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS. |
| Immunogen | Synthetic peptide of Rat Protein Kinase A regulatory subunit I beta. |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Recommended Dilutions | Recommended dilutions WB: 1/1,000 when using colorimetric substrates such as BCIP/NBT and 1/5,000 for chemiluminescent substrates. Higher concentration of antibody may be needed for non-human samples. Predicted molecular weight: 43 kDa. This antibody identifies bands at 55 kD and 53 kD when used against the reduced protein as well as activation/breakdown products_recover. The antibody can recognize native or reduced PKARIb. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Storage Buffer | phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage Condition | This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt. |
| Formulation | Liquid |
| Species Reactivity | Human,Mouse,Rat,Other |
| Specificity | The antibody binds to PKARIb, but does not cross react with the other PKA regulatory subunits (PKARIa, PKARIIa, PKARIIb). |
| Purity | Immunogen Affinity purified |
| Guarantee | abm guarantees that all our Anti-PKA-RIB (Protein Kinase-A Regulatory Subunit Ib) Antibody will perform as described on this product webpage, if this is not the case we will provide you with a one-time replacement at no extra cost. Documentation and explanation of experiment conducted will be required when submitting a claim for replacement. |
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Y051648 |
Print & Download Datasheet
Search CoA here
There are no Documents for this product yet!
There are no FAQs for this product yet!
There are no references for this product yet!
This product has no review yet.
Browse Other Antibodies:
Select Rasied In:
Armenian hamster
(0)
Bovine
(0)
Chicken
(0)
Donkey
(0)
Goat
(0)
Golden Syrian Hamster
(0)
Guinea pig
(0)
Hamster
(0)
Horse
(0)
Human
(0)
Mouse
(0)
Other
(0)
Rabbit
(0)
Rat
(0)
Sheep
(0)
Select Reactivity:
Avian
(0)
Bombyx mori
(0)
Bovine
(0)
C. elegans
(0)
Chicken
(0)
CMV
(0)
Dog
(0)
Drosophila
(0)
E. coli
(0)
Fusarium sepsis
(0)
Hamster
(0)
HBV
(0)
Horse
(0)
Human
(0)
Llama
(0)
Locusta migratoria
(0)
Manduca
(0)
Mosquito
(0)
Mouse
(0)
Notapplicable
(0)
Other
(0)
Pig
(0)
Planaria
(0)
Porcine
(0)
Quail
(0)
Rat
(0)
Rodent
(0)
Sheep
(0)
Species independent
(0)
Spodoptera frugiperda
(0)
Xenopus
(0)
Zebrafish
(0)