OneScript® Plus (MMLV) Reverse Transcriptase
| Cat. No. | G237 | ||||||||||
| Name | OneScript® Plus (MMLV) Reverse Transcriptase | ||||||||||
| Unit | 100 rxn | ||||||||||
| Category | Reverse Transcriptase & RT-PCR | ||||||||||
| Description |
OneScript® Plus MMLV Reverse Transcriptase is a Moloney-Murine Leukemia Virus(MMLV) Reverse Transcriptase with genetic modifications to abolish RNase H activity to achieve thermal stability. This special mutant enzyme offers higher cDNA yields, longer cDNA up to 12 kb, and is able to perform under high temperatures (50°C - 55°C), facilitating the elimination of secondary structures associated with GC-rich RNA templates. OneScript® Plus is formulated with abm’s RNaseOFF Ribonuclease Inhibitor (Cat. No. G138) offering improved resistance to oxidation compared to the high oxidation-sensitive human RNase inhibitors. RNaseOFF is stable even under very low concentrations of DTT (< 1 mM), making it the best choice for ultimate RNA protection. Product Features:
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| Application |
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| Storage Condition |
Store at -20°C. |
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| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G237 |
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Can I use both total RNA and poly(A)+ mRNA for cDNA synthesis?
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Yes, both total RNA and poly(A)+ mRNA can be used, though poly(A)+ mRNA typically yields higher quantities and better purity.
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| What can I use the synthesized cDNA for? | |
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The high-quality cDNA can be used in a variety of downstream applications, including gene expression analysis, cloning, and PCR-based assays. BlasTaq™ 2X qPCR Master Mix (Cat. No. G891) is well suited to downstream qPCR applications.
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| How should I store the synthesized cDNA? | |
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Store the synthesized first-strand cDNA at -20°C for long-term use.
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| Why am I getting low cDNA yields? | |
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Low cDNA yields can be caused by poor RNA integrity, contamination, or insufficient RNA input. To improve yield:
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| How much RNA template should I use for cDNA synthesis? | |
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We recommend using 1 ng to 2 μg of RNA per reaction.
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What volumes of cDNA should I use for downstream PCR?
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Typically, use 1 μl of cDNA in a 25 μl PCR reaction. You can add up to 20% of the PCR volume (e.g., 5 μl in a 25 μl PCR), depending on your target and primers.
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- Menon, V et al. "The contribution of visceral fat to improved insulin signaling in Ames dwarf mice" Aging Cell 13 (3):497-506 (2014). DOI: 10.1111/acel.12201. PubMed: 24690289. Application: Reverse Transcription.
- Correnti, JM et al. "Adiponectin fine-tuning of liver regeneration dynamics revealed through cellular networkmodeling" J Physiol. : (2014). PubMed: 25384784.
- Correnti, JM et al. "Adiponectin fine-tuning of liver regeneration dynamics revealed through cellular network modelling" J Physiol 593(2):365-383 (2015). DOI: 10.1113/jphysiol.2014.284109. PubMed: 25630259. Application: Reverse Transcription.
- Guo, C., Wang, X., Zhang, H., Zhi, L., Lv, T., Li, M., … Zhu, W. "Structure-based rational design of a novel chimeric PD1-NKG2D receptor for natural killer cells" Molecular Immunology 114:108–113 (2019). DOI: 10.1016/j.molimm.2019.07.009.