OneScript® Hot Reverse Transcriptase
| Cat. No. | G593 | ||||||||
| Name | OneScript® Hot Reverse Transcriptase | ||||||||
| Unit | 100 rxn | ||||||||
| Category | Reverse Transcriptase & RT-PCR | ||||||||
| Description |
OneScript® Hot (thermostable) Reverse Transcriptase is a mutational derivative of Moloney-Murine Leukemia Virus Reverse Transcriptase, that can reverse transcribe low abundance or degraded RNA, and has significantly better resistance to contaminating inhibitors such as reagents used during RNA extraction and contaminants from biological samples. High processivity and sensitivity allow for rapid cDNA synthesis of full-length cDNA fragments in a fraction of the time of leading competitors. abm is the only company in the world to have a thermostable reverse transcriptase engineered to offer superior cDNA synthesis performance with even the most challenging RNA samples due to its incredible thermostability at 60-72ºC. This is the enzyme of choice for daily or demanding RNA reverse transcription. OneScript® Hot is formulated with abm’s RNaseOFF Ribonuclease Inhibitor offering improved resistance to oxidation compared to the high oxidation-sensitive human RNase inhibitors. RNaseOFF is stable even under very low concentrations of DTT (< 1 mM), making it the best choice for ultimate RNA protection. Product Features:
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| Storage Condition |
Store at -20ºC. |
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| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G593 |
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Can I use both total RNA and poly(A)+ mRNA for cDNA synthesis?
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Yes, both total RNA and poly(A)+ mRNA can be used, though poly(A)+ mRNA typically yields higher quantities and better purity.
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Can I synthesize cDNA from long RNA transcripts?
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Yes, for longer RNA transcripts, extend the 60°C incubation time to up to 30 minutes.
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| What can I use the synthesized cDNA for? | |
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The high-quality cDNA can be used in a variety of downstream applications, including gene expression analysis, cloning, and PCR-based assays. BlasTaq™ 2X qPCR Master Mix (Cat. No. G891) is well suited to downstream qPCR applications.
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| How should I store the synthesized cDNA? | |
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Store the synthesized first-strand cDNA at -20°C for long-term use.
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| Why am I getting low cDNA yields? | |
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Low cDNA yields can be caused by poor RNA integrity, contamination, or insufficient RNA input. To improve yield:
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| How much RNA template should I use for cDNA synthesis? | |
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We recommend using 1 ng to 2 μg of RNA per reaction.
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What volumes of cDNA should I use for downstream PCR?
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Typically, use 1 μl of cDNA in a 25 μl PCR reaction. You can add up to 20% of the PCR volume (e.g., 5 μl in a 25 μl PCR), depending on your target and primers.
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