Ultra-Pure Lentivirus Purification Kit

Recombinant lentivirus is widely used in the transduction of a variety of target cells and can be produced by transient transfection, from which viral supernatant can be collected and used for target cell transduction. The titre of viral supernatant by transient transfection is approximately 10⁶ cfu (colony forming unit)/ml, which is sufficient for the generation of most stable cell lines in vitro. However, there are applications that require higher purity and titres. These applications include experiments demanding higher gene transduction efficiency and in vivo gene delivery. In addition, crude viral supernatants are not suitable for in vivo administration due to various contaminants contained in cell culture supernatant. Thus, the viral supernatant needs to be further concentrated and purified before use. Traditionally, recombinant lentivirus has been concentrated via ultracentrifugation. Although the method works well, it requires specific ultracentrifugation equipment and it is technically demanding. In addition, the total viral supernatant volume to be concentrated is limited to the size of ultracentrifugation tubes. Reports have also stated that the ultracentrifugation process has some detrimental physical effects on the biological activity of purified viral particles. Due to limitations of the existing technique, there is great need for a quick and efficient method to purify and concentrate recombinant lentivirus. Scientists at abm Inc. have developed the unique Ultra-Pure Lentivirus Purification Kit, a one of a kind product in the industry. The kit utilizes a proprietary Lentivirus binding matrix, binding and elution buffers based on ion-exchange, which minimizes damage to the viral particles while enabling researchers to attain ultra-high titres.

This Ultra-Pure Lentivirus Purification Kit contains enough solutions and buffers required to purify virus from up to 1.6L (16 x 100 ml samples) of crude virus supernatant:

• Materials Provided in Kit:
  • LentiBind Buffer A (Cat. No. LV998-1, 320ml)
  • LentiBind Buffer B (Cat. No. LV998-2, 128ml)
  • Lenti-Elution buffer C (Cat. No. LV998-3, 32ml)
  • LentiBuffer D (Cat. No. LV998-4, 40ml)
• Other Required Materials/Equipment (user provided):
  • 15ml sterile centrifuge tube (Sarstedt)
  • Low protein binding, 0.45µM filter. (Millipore, Cat#SLHVR13SL)
  • 100kDa cut-off buffer exchange column (Sartorius, Cat# VS2041)
  • Centrifuge
  • Orbital/rotating shaker

Download the User Manual here.