pAAV-CMV-DIO-Blank-SV40-Puro
| Vector Name | pAAV-CMV-DIO-Blank-SV40-Puro |
| VectorType | AAV Vector |
| Antibiotic Information | Bacterial: KanamycinMammalian: Puromycin |
| Sequencing Primers | CMV sequencing primer 5`---CGC AAA TGG GCG GTA GGC GTG---3` |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta acctgcgcgc tcgctcgctc
481 actgaggccg cccgggcaaa gcccgggcgt cgggcgacct ttggtcgccc ggcctcagtg
541 agcgagcgag cgcgcagaga gggagtggcc aactccatca ctaggggttc cttgtagtta
601 atgattaacc cgccatgcta cttatctacg tagccatgct ctaggaagat cgcctaggcg
661 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
721 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
781 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
841 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
901 catgacctta tgggactttc ctacttggca gtacatctac gtttagtcat cgctattacc
961 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
1021 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
1081 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
1141 cggtgggagg tctatataag cagagctggt accaatcagc tcgctcacca tataacttcg
1201 tataaagtat cctatacgaa gttatatcaa aataggaaga ccaatgcttc accatcgacc
1261 cgaattgcca agcatcacca tcgacccata acttcgtata atgtatgcta tacgaagtta
1321 tctcgagtca gatatcgcta gccaattgat aacttcgtat aggatacttt atacgaagtt
1381 atcattggga ttcttcctat tttgatccaa gcatcaccat cgaccctcta gtccagatct
1441 caccatcgac ccataacttc gtatagcata cattatacga agttatatgg tgagcaaggg
1501 cgattctaga aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
1561 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
1621 caatgtatct taaagcttgg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
1681 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
1741 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
1801 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
1861 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
1921 gaggaggctt ttttggaggc catgaccgag tacaagccca cggtgcgcct cgccacccgc
1981 gacgacgtcc ctcgggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
2041 cgccacaccg tggacccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
2101 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
2161 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
2221 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga agggctcctg
2281 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
2341 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
2401 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
2461 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
2521 acccgcaagc ccggtgcctg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
2581 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
2641 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
2701 aggacagcaa gggggaggat tgggaagaga atagcaggca tgctggggaa agcttaggaa
2761 cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg
2821 cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg
2881 cgcagggatc ccggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
2941 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
3001 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
3061 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
3121 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
3181 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
3241 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
3301 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
3361 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
3421 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
3481 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
3541 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
3601 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
3661 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
3721 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
3781 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
3841 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
3901 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
3961 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
4021 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
4081 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
4141 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gaaaaaggat
4201 cttcacctag atccttttca cgtagaaagc cagtccgcag aaacggtgct gaccccggat
4261 gaatgtcagc tactgggcta tctggacaag ggaaaacgca agcgcaaaga gaaagcaggt
4321 agcttgcagt gggcttacat ggcgatagct agactgggcg gttttatgga cagcaagcga
4381 accggaattg ccagctgggg cgccctctgg taaggttggg aagccctgca aagtaaactg
4441 gatggctttc tcgccgccaa ggatctgatg gcgcagggga tcaagctctg atcaagagac
4501 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc
4561 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
4621 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
4681 cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg
4741 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
4801 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
4861 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
4921 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
4981 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
5041 caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
5101 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
5161 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
5221 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
5281 cgccttctat cgccttcttg acgagttctt ctgaattttg ttaaaatttt tgttaaatca
5341 gctcattttt taaccaatag gccgaaatcg gcaacatccc ttataaatca aaagaataga
5401 ccgcgatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
5461 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
5521 cacccaaatc aagttttttg cggtcgaggt gccgtaaagc tctaaatcgg aaccctaaag
5581 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
5641 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
5701 ccaccacacc cgcgcgctta atgcgccgct acagggcgcg tccattcgcc attcaggatc
5761 gaattaattc ttaattaaca tcatcaataa tatacctt
LOCUS dna 5798 bp
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
misc_binding 457..462
/dbxref="REBASE:HpaI"
misc_binding 457..462
/dbxref="REBASE:HincII"
misc_binding 833..838
/dbxref="REBASE:NdeI"
misc_binding 1168..1173
/dbxref="REBASE:KpnI"
Other Gene 1288..1321
/gene="loxP other"
misc_binding 1322..1327
/dbxref="REBASE:XhoI"
misc_binding 1331..1336
/dbxref="REBASE:EcoRV"
misc_binding 1435..1440
/dbxref="REBASE:BglII"
Other Gene 1453..1486
/gene="loxP other"
misc_binding 1505..1510
/dbxref="REBASE:XbaI"
Promoter 1639..1907
/gene="SV40 prom"
Rep_Origin 1806..1883
/gene="SV40 origin"
ORF 1873..2541
/sequence="ORF_1 rf(1)"
misc_binding 1886..1898
/dbxref="REBASE:SfiI"
Marker 1942..2541
/gene="puro marker"
misc_binding 2886..2891
/dbxref="REBASE:BamHI"
Rep_Origin 3110..3729
/gene="pBR322 origin"
Promoter 4382..4431
/gene="NEOKAN prom"
misc_binding 4489..4494
/dbxref="REBASE:BclI"
ORF 4520..5314
/sequence="ORF_2 rf(2)"
Marker 4523..5311
/gene="NTP_II marker"
Rep_Origin 5419..5724
/gene="f1 origin"
BASE COUNT 1359 a 1547 c 1583 g 1309 t 0 others
ORIGIN
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta acctgcgcgc tcgctcgctc
481 actgaggccg cccgggcaaa gcccgggcgt cgggcgacct ttggtcgccc ggcctcagtg
541 agcgagcgag cgcgcagaga gggagtggcc aactccatca ctaggggttc cttgtagtta
601 atgattaacc cgccatgcta cttatctacg tagccatgct ctaggaagat cgcctaggcg
661 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
721 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
781 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
841 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
901 catgacctta tgggactttc ctacttggca gtacatctac gtttagtcat cgctattacc
961 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
1021 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
1081 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
1141 cggtgggagg tctatataag cagagctggt accaatcagc tcgctcacca tataacttcg
1201 tataaagtat cctatacgaa gttatatcaa aataggaaga ccaatgcttc accatcgacc
1261 cgaattgcca agcatcacca tcgacccata acttcgtata atgtatgcta tacgaagtta
1321 tctcgagtca gatatcgcta gccaattgat aacttcgtat aggatacttt atacgaagtt
1381 atcattggga ttcttcctat tttgatccaa gcatcaccat cgaccctcta gtccagatct
1441 caccatcgac ccataacttc gtatagcata cattatacga agttatatgg tgagcaaggg
1501 cgattctaga aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
1561 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
1621 caatgtatct taaagcttgg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
1681 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
1741 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
1801 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
1861 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
1921 gaggaggctt ttttggaggc catgaccgag tacaagccca cggtgcgcct cgccacccgc
1981 gacgacgtcc ctcgggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
2041 cgccacaccg tggacccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
2101 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
2161 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
2221 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga agggctcctg
2281 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
2341 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
2401 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
2461 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
2521 acccgcaagc ccggtgcctg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
2581 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
2641 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
2701 aggacagcaa gggggaggat tgggaagaga atagcaggca tgctggggaa agcttaggaa
2761 cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg
2821 cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg
2881 cgcagggatc ccggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
2941 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
3001 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
3061 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
3121 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
3181 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
3241 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
3301 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
3361 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
3421 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
3481 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
3541 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
3601 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
3661 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
3721 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
3781 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
3841 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
3901 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
3961 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
4021 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
4081 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
4141 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gaaaaaggat
4201 cttcacctag atccttttca cgtagaaagc cagtccgcag aaacggtgct gaccccggat
4261 gaatgtcagc tactgggcta tctggacaag ggaaaacgca agcgcaaaga gaaagcaggt
4321 agcttgcagt gggcttacat ggcgatagct agactgggcg gttttatgga cagcaagcga
4381 accggaattg ccagctgggg cgccctctgg taaggttggg aagccctgca aagtaaactg
4441 gatggctttc tcgccgccaa ggatctgatg gcgcagggga tcaagctctg atcaagagac
4501 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc
4561 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
4621 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
4681 cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg
4741 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
4801 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
4861 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
4921 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
4981 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
5041 caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
5101 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
5161 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
5221 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
5281 cgccttctat cgccttcttg acgagttctt ctgaattttg ttaaaatttt tgttaaatca
5341 gctcattttt taaccaatag gccgaaatcg gcaacatccc ttataaatca aaagaataga
5401 ccgcgatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
5461 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
5521 cacccaaatc aagttttttg cggtcgaggt gccgtaaagc tctaaatcgg aaccctaaag
5581 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
5641 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
5701 ccaccacacc cgcgcgctta atgcgccgct acagggcgcg tccattcgcc attcaggatc
5761 gaattaattc ttaattaaca tcatcaataa tatacctt
//
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 ttttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgt 60
AAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCA
61 gggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaa 120
CCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTT
Encap other(162,310)>>>
|
121 cacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgccggtgtacacagg 180
GTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCC
181 aagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagt 240
TTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCA
241 aagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttg 300
TTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAAC
301 tgttactcatagcgcgtaatacggcagacctcagcgctagattattgaagcatttatcag 360
ACAATGAGTATCGCGCATTATGCCGTCTGGAGTCGCGATCTAATAACTTCGTAAATAGTC
amp prom(365,393)<<<
|
361 ggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggg 420
CCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCC
HincII
|
HpaI
|
421 gttccgcgcacatttccccgaaaagtgccacctgacgttaacctgcgcgctcgctcgctc 480
CAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAATTGGACGCGCGAGCGAGCGAG
481 actgaggccgcccgggcaaagcccgggcgtcgggcgacctttggtcgcccggcctcagtg 540
TGACTCCGGCGGGCCCGTTTCGGGCCCGCAGCCCGCTGGAAACCAGCGGGCCGGAGTCAC
541 agcgagcgagcgcgcagagagggagtggccaactccatcactaggggttccttgtagtta 600
TCGCTCGCTCGCGCGTCTCTCCCTCACCGGTTGAGGTAGTGATCCCCAAGGAACATCAAT
601 atgattaacccgccatgctacttatctacgtagccatgctctaggaagatcgcctaggcg 660
TACTAATTGGGCGGTACGATGAATAGATGCATCGGTACGAGATCCTTCTAGCGGATCCGC
661 cgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccatt 720
GCAATGTATTGAATGCCATTTACCGGGCGGACCGACTGGCGGGTTGCTGGGGGCGGGTAA
721 gacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtca 780
CTGCAGTTATTACTGCATACAAGGGTATCATTGCGGTTATCCCTGAAAGGTAACTGCAGT
NdeI
|
781 atgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgcc 840
TACCCACCTCATAAATGCCATTTGACGGGTGAACCGTCATGTAGTTCACATAGTATACGG
841 aagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagta 900
TTCATGCGGGGGATAACTGCAGTTACTGCCATTTACCGGGCGGACCGTAATACGGGTCAT
901 catgaccttatgggactttcctacttggcagtacatctacgtttagtcatcgctattacc 960
GTACTGGAATACCCTGAAAGGATGAACCGTCATGTAGATGCAAATCAGTAGCGATAATGG
961 atggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacgggga 1020
TACCACTACGCCAAAACCGTCATGTAGTTACCCGCACCTATCGCCAAACTGAGTGCCCCT
1021 tttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgg 1080
AAAGGTTCAGAGGTGGGGTAACTGCAGTTACCCTCAAACAAAACCGTGGTTTTAGTTGCC
1081 gactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgta 1140
CTGAAAGGTTTTACAGCATTGTTGAGGCGGGGTAACTGCGTTTACCCGCCATCCGCACAT
KpnI
|
1141 cggtgggaggtctatataagcagagctggtaccaatcagctcgctcaccatataacttcg 1200
GCCACCCTCCAGATATATTCGTCTCGACCATGGTTAGTCGAGCGAGTGGTATATTGAAGC
1201 tataaagtatcctatacgaagttatatcaaaataggaagaccaatgcttcaccatcgacc 1260
ATATTTCATAGGATATGCTTCAATATAGTTTTATCCTTCTGGTTACGAAGTGGTAGCTGG
loxP other(1288,1321)<<<
|
1261 cgaattgccaagcatcaccatcgacccataacttcgtataatgtatgctatacgaagtta 1320
GCTTAACGGTTCGTAGTGGTAGCTGGGTATTGAAGCATATTACATACGATATGCTTCAAT
XhoI EcoRV
| |
1321 tctcgagtcagatatcgctagccaattgataacttcgtataggatactttatacgaagtt 1380
AGAGCTCAGTCTATAGCGATCGGTTAACTATTGAAGCATATCCTATGAAATATGCTTCAA
BglII
|
1381 atcattgggattcttcctattttgatccaagcatcaccatcgaccctctagtccagatct 1440
TAGTAACCCTAAGAAGGATAAAACTAGGTTCGTAGTGGTAGCTGGGAGATCAGGTCTAGA
loxP other(1453,1486)>>>
|
1441 caccatcgacccataacttcgtatagcatacattatacgaagttatatggtgagcaaggg 1500
GTGGTAGCTGGGTATTGAAGCATATCGTATGTAATATGCTTCAATATACCACTCGTTCCC
XbaI
|
1501 cgattctagaaacttgtttattgcagcttataatggttacaaataaagcaatagcatcac 1560
GCTAAGATCTTTGAACAAATAACGTCGAATATTACCAATGTTTATTTCGTTATCGTAGTG
1561 aaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcat 1620
TTTAAAGTGTTTATTTCGTAAAAAAAGTGACGTAAGATCAACACCAAACAGGTTTGAGTA
SV40 prom(1639,1907)>>>
|
1621 caatgtatcttaaagcttggtgtggaaagtccccaggctccccagcaggcagaagtatgc 1680
GTTACATAGAATTTCGAACCACACCTTTCAGGGGTCCGAGGGGTCGTCCGTCTTCATACG
1681 aaagcatgcatctcaattagtcagcaaccaggtgtggaaagtccccaggctccccagcag 1740
TTTCGTACGTAGAGTTAATCAGTCGTTGGTCCACACCTTTCAGGGGTCCGAGGGGTCGTC
1741 gcagaagtatgcaaagcatgcatctcaattagtcagcaaccatagtcccgcccctaactc 1800
CGTCTTCATACGTTTCGTACGTAGAGTTAATCAGTCGTTGGTATCAGGGCGGGGATTGAG
SV40 origin(1806,1883)>>>
|
1801 cgcccatcccgcccctaactccgcccagttccgcccattctccgccccatggctgactaa 1860
GCGGGTAGGGCGGGGATTGAGGCGGGTCAAGGCGGGTAAGAGGCGGGGTACCGACTGATT
SfiI
|
ORF_1 rf(1)(1873,2541)>>>
| |
1861 ttttttttatttatgcagaggccgaggccgcctcggcctctgagctattccagaagtagt 1920
AAAAAAAATAAATACGTCTCCGGCTCCGGCGGAGCCGGAGACTCGATAAGGTCTTCATCA
puro marker(1942,2541)>>>
|
1921 gaggaggcttttttggaggccatgaccgagtacaagcccacggtgcgcctcgccacccgc 1980
CTCCTCCGAAAAAACCTCCGGTACTGGCTCATGTTCGGGTGCCACGCGGAGCGGTGGGCG
1981 gacgacgtccctcgggccgtacgcaccctcgccgccgcgttcgccgactaccccgccacg 2040
CTGCTGCAGGGAGCCCGGCATGCGTGGGAGCGGCGGCGCAAGCGGCTGATGGGGCGGTGC
2041 cgccacaccgtggacccggaccgccacatcgagcgggtcaccgagctgcaagaactcttc 2100
GCGGTGTGGCACCTGGGCCTGGCGGTGTAGCTCGCCCAGTGGCTCGACGTTCTTGAGAAG
2101 ctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtg 2160
GAGTGCGCGCAGCCCGAGCTGTAGCCGTTCCACACCCAGCGCCTGCTGCCGCGGCGCCAC
2161 gcggtctggaccacgccggagagcgtcgaagcgggggcggtgttcgccgagatcggcccg 2220
CGCCAGACCTGGTGCGGCCTCTCGCAGCTTCGCCCCCGCCACAAGCGGCTCTAGCCGGGC
2221 cgcatggccgagttgagcggttcccggctggccgcgcagcaacagatggaagggctcctg 2280
GCGTACCGGCTCAACTCGCCAAGGGCCGACCGGCGCGTCGTTGTCTACCTTCCCGAGGAC
2281 gcgccgcaccggcccaaggagcccgcgtggttcctggccaccgtcggcgtctcgcccgac 2340
CGCGGCGTGGCCGGGTTCCTCGGGCGCACCAAGGACCGGTGGCAGCCGCAGAGCGGGCTG
2341 caccagggcaagggtctgggcagcgccgtcgtgctccccggagtggaggcggccgagcgc 2400
GTGGTCCCGTTCCCAGACCCGTCGCGGCAGCACGAGGGGCCTCACCTCCGCCGGCTCGCG
2401 gccggggtgcccgccttcctggagacctccgcgccccgcaacctccccttctacgagcgg 2460
CGGCCCCACGGGCGGAAGGACCTCTGGAGGCGCGGGGCGTTGGAGGGGAAGATGCTCGCC
2461 ctcggcttcaccgtcaccgccgacgtcgaggtgcccgaaggaccgcgcacctggtgcatg 2520
GAGCCGAAGTGGCAGTGGCGGCTGCAGCTCCACGGGCTTCCTGGCGCGTGGACCACGTAC
2521 acccgcaagcccggtgcctgactgtgccttctagttgccagccatctgttgtttgcccct 2580
TGGGCGTTCGGGCCACGGACTGACACGGAAGATCAACGGTCGGTAGACAACAAACGGGGA
2581 cccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatg 2640
GGGGGCACGGAAGGAACTGGGACCTTCCACGGTGAGGGTGACAGGAAAGGATTATTTTAC
2641 aggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggc 2700
TCCTTTAACGTAGCGTAACAGACTCATCCACAGTAAGATAAGACCCCCCACCCCACCCCG
2701 aggacagcaagggggaggattgggaagagaatagcaggcatgctggggaaagcttaggaa 2760
TCCTGTCGTTCCCCCTCCTAACCCTTCTCTTATCGTCCGTACGACCCCTTTCGAATCCTT
2761 cccctagtgatggagttggccactccctctctgcgcgctcgctcgctcactgaggccggg 2820
GGGGATCACTACCTCAACCGGTGAGGGAGAGACGCGCGAGCGAGCGAGTGACTCCGGCCC
2821 cgaccaaaggtcgcccgacgcccgggctttgcccgggcggcctcagtgagcgagcgagcg 2880
GCTGGTTTCCAGCGGGCTGCGGGCCCGAAACGGGCCCGCCGGAGTCACTCGCTCGCTCGC
BamHI
|
2881 cgcagggatcccggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggc 2940
GCGTCCCTAGGGCCACACTTTATGGCGTGTCTACGCATTCCTCTTTTATGGCGTAGTCCG
2941 gctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcgg 3000
CGAGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGAGCCAGCAAGCCGACGCCGCTCGCC
3001 tatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaa 3060
ATAGTCGAGTGAGTTTCCGCCATTATGCCAATAGGTGTCTTAGTCCCCTATTGCGTCCTT
pBR322 origin(3110,3729)<<<
|
3061 agaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctgg 3120
TCTTGTACACTCGTTTTCCGGTCGTTTTCCGGTCCTTGGCATTTTTCCGGCGCAACGACC
3121 cgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcaga 3180
GCAAAAAGGTATCCGAGGCGGGGGGACTGCTCGTAGTGTTTTTAGCTGCGAGTTCAGTCT
3181 ggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcg 3240
CCACCGCTTTGGGCTGTCCTGATATTTCTATGGTCCGCAAAGGGGGACCTTCGAGGGAGC
3241 tgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgg 3300
ACGCGAGAGGACAAGGCTGGGACGGCGAATGGCCTATGGACAGGCGGAAAGAGGGAAGCC
3301 gaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttc 3360
CTTCGCACCGCGAAAGAGTATCGAGTGCGACATCCATAGAGTCAAGCCACATCCAGCAAG
3361 gctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccg 3420
CGAGGTTCGACCCGACACACGTGCTTGGGGGGCAAGTCGGGCTGGCGACGCGGAATAGGC
3421 gtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagcca 3480
CATTGATAGCAGAACTCAGGTTGGGCCATTCTGTGCTGAATAGCGGTGACCGTCGTCGGT
3481 ctggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggt 3540
GACCATTGTCCTAATCGTCTCGCTCCATACATCCGCCACGATGTCTCAAGAACTTCACCA
3541 ggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccag 3600
CCGGATTGATGCCGATGTGATCTTCCTGTCATAAACCATAGACGCGAGACGACTTCGGTC
3601 ttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcg 3660
AATGGAAGCCTTTTTCTCAACCATCGAGAACTAGGCCGTTTGTTTGGTGGCGACCATCGC
3661 gtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatc 3720
CACCAAAAAAACAAACGTTCGTCGTCTAATGCGCGTCTTTTTTTCCTAGAGTTCTTCTAG
3721 ctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattt 3780
GAAACTAGAAAAGATGCCCCAGACTGCGAGTCACCTTGCTTTTGAGTGCAATTCCCTAAA
3781 tggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagtt 3840
ACCAGTACTCTAATAGTTTTTCCTAGAAGTGGATCTAGGAAAATTTAATTTTTACTTCAA
3841 ttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatca 3900
AATTTAGTTAGATTTCATATATACTCATTTGAACCAGACTGTCAATGGTTACGAATTAGT
3901 gtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccg 3960
CACTCCGTGGATAGAGTCGCTAGACAGATAAAGCAAGTAGGTATCAACGGACTGAGGGGC
3961 tcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgatac 4020
AGCACATCTATTGATGCTATGCCCTCCCGAATGGTAGACCGGGGTCACGACGTTACTATG
4021 cgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaaggg 4080
GCGCTCTGGGTGCGAGTGGCCGAGGTCTAAATAGTCGTTATTTGGTCGGTCGGCCTTCCC
4081 ccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgcc 4140
GGCTCGCGTCTTCACCAGGACGTTGAAATAGGCGGAGGTAGGTCAGATAATTAACAACGG
4141 gggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgaaaaaggat 4200
CCCTTCGATCTCATTCATCAAGCGGTCAATTATCAAACGCGTTGCAACAACTTTTTCCTA
4201 cttcacctagatccttttcacgtagaaagccagtccgcagaaacggtgctgaccccggat 4260
GAAGTGGATCTAGGAAAAGTGCATCTTTCGGTCAGGCGTCTTTGCCACGACTGGGGCCTA
4261 gaatgtcagctactgggctatctggacaagggaaaacgcaagcgcaaagagaaagcaggt 4320
CTTACAGTCGATGACCCGATAGACCTGTTCCCTTTTGCGTTCGCGTTTCTCTTTCGTCCA
4321 agcttgcagtgggcttacatggcgatagctagactgggcggttttatggacagcaagcga 4380
TCGAACGTCACCCGAATGTACCGCTATCGATCTGACCCGCCAAAATACCTGTCGTTCGCT
NEOKAN prom(4382,4431)>>>
|
4381 accggaattgccagctggggcgccctctggtaaggttgggaagccctgcaaagtaaactg 4440
TGGCCTTAACGGTCGACCCCGCGGGAGACCATTCCAACCCTTCGGGACGTTTCATTTGAC
BclI
|
4441 gatggctttctcgccgccaaggatctgatggcgcaggggatcaagctctgatcaagagac 4500
CTACCGAAAGAGCGGCGGTTCCTAGACTACCGCGTCCCCTAGTTCGAGACTAGTTCTCTG
NTP_II marker(4523,5311)>>>
|
ORF_2 rf(2)(4520,5314)>>>
| |
4501 aggatgaggatcgtttcgcatgattgaacaagatggattgcacgcaggttctccggccgc 4560
TCCTACTCCTAGCAAAGCGTACTAACTTGTTCTACCTAACGTGCGTCCAAGAGGCCGGCG
4561 ttgggtggagaggctattcggctatgactgggcacaacagacaatcggctgctctgatgc 4620
AACCCACCTCTCCGATAAGCCGATACTGACCCGTGTTGTCTGTTAGCCGACGAGACTACG
4621 cgccgtgttccggctgtcagcgcaggggcgcccggttctttttgtcaagaccgacctgtc 4680
GCGGCACAAGGCCGACAGTCGCGTCCCCGCGGGCCAAGAAAAACAGTTCTGGCTGGACAG
4681 cggtgccctgaatgaactgcaagacgaggcagcgcggctatcgtggctggccacgacggg 4740
GCCACGGGACTTACTTGACGTTCTGCTCCGTCGCGCCGATAGCACCGACCGGTGCTGCCC
4741 cgttccttgcgcagctgtgctcgacgttgtcactgaagcgggaagggactggctgctatt 4800
GCAAGGAACGCGTCGACACGAGCTGCAACAGTGACTTCGCCCTTCCCTGACCGACGATAA
4801 gggcgaagtgccggggcaggatctcctgtcatctcaccttgctcctgccgagaaagtatc 4860
CCCGCTTCACGGCCCCGTCCTAGAGGACAGTAGAGTGGAACGAGGACGGCTCTTTCATAG
4861 catcatggctgatgcaatgcggcggctgcatacgcttgatccggctacctgcccattcga 4920
GTAGTACCGACTACGTTACGCCGCCGACGTATGCGAACTAGGCCGATGGACGGGTAAGCT
4921 ccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaagccggtcttgtcga 4980
GGTGGTTCGCTTTGTAGCGTAGCTCGCTCGTGCATGAGCCTACCTTCGGCCAGAACAGCT
4981 tcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactgttcgccaggct 5040
AGTCCTACTAGACCTGCTTCTCGTAGTCCCCGAGCGCGGTCGGCTTGACAAGCGGTCCGA
5041 caaggcgagcatgcccgacggcgaggatctcgtcgtgacccatggcgatgcctgcttgcc 5100
GTTCCGCTCGTACGGGCTGCCGCTCCTAGAGCAGCACTGGGTACCGCTACGGACGAACGG
5101 gaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtgt 5160
CTTATAGTACCACCTTTTACCGGCGAAAAGACCTAAGTAGCTGACACCGGCCGACCCACA
5161 ggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcgg 5220
CCGCCTGGCGATAGTCCTGTATCGCAACCGATGGGCACTATAACGACTTCTCGAACCGCC
5221 cgaatgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcat 5280
GCTTACCCGACTGGCGAAGGAGCACGAAATGCCATAGCGGCGAGGGCTAAGCGTCGCGTA
5281 cgccttctatcgccttcttgacgagttcttctgaattttgttaaaatttttgttaaatca 5340
GCGGAAGATAGCGGAAGAACTGCTCAAGAAGACTTAAAACAATTTTAAAAACAATTTAGT
5341 gctcattttttaaccaataggccgaaatcggcaacatcccttataaatcaaaagaataga 5400
CGAGTAAAAAATTGGTTATCCGGCTTTAGCCGTTGTAGGGAATATTTAGTTTTCTTATCT
f1 origin(5419,5724)<<<
|
5401 ccgcgatagggttgagtgttgttccagtttggaacaagagtccactattaaagaacgtgg 5460
GGCGCTATCCCAACTCACAACAAGGTCAAACCTTGTTCTCAGGTGATAATTTCTTGCACC
5461 actccaacgtcaaagggcgaaaaaccgtctatcagggcgatggcccactacgtgaaccat 5520
TGAGGTTGCAGTTTCCCGCTTTTTGGCAGATAGTCCCGCTACCGGGTGATGCACTTGGTA
5521 cacccaaatcaagttttttgcggtcgaggtgccgtaaagctctaaatcggaaccctaaag 5580
GTGGGTTTAGTTCAAAAAACGCCAGCTCCACGGCATTTCGAGATTTAGCCTTGGGATTTC
5581 ggagcccccgatttagagcttgacggggaaagccggcgaacgtggcgagaaaggaaggga 5640
CCTCGGGGGCTAAATCTCGAACTGCCCCTTTCGGCCGCTTGCACCGCTCTTTCCTTCCCT
5641 agaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcggtcacgctgcgcgtaa 5700
TCTTTCGCTTTCCTCGCCCGCGATCCCGCGACCGTTCACATCGCCAGTGCGACGCGCATT
5701 ccaccacacccgcgcgcttaatgcgccgctacagggcgcgtccattcgccattcaggatc 5760
GGTGGTGTGGGCGCGCGAATTACGCGGCGATGTCCCGCGCAGGTAAGCGGTAAGTCCTAG
5761 gaattaattcttaattaacatcatcaataatatacctt 5798
CTTAATTAAGAATTAATTGTAGTAGTTATTATATGGAA
LOCUS dna 5798 bp
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
misc_binding 457..462
/dbxref="REBASE:HpaI"
misc_binding 457..462
/dbxref="REBASE:HincII"
misc_binding 833..838
/dbxref="REBASE:NdeI"
misc_binding 1168..1173
/dbxref="REBASE:KpnI"
Other Gene 1288..1321
/gene="loxP other"
misc_binding 1322..1327
/dbxref="REBASE:XhoI"
misc_binding 1331..1336
/dbxref="REBASE:EcoRV"
misc_binding 1435..1440
/dbxref="REBASE:BglII"
Other Gene 1453..1486
/gene="loxP other"
misc_binding 1505..1510
/dbxref="REBASE:XbaI"
Promoter 1639..1907
/gene="SV40 prom"
Rep_Origin 1806..1883
/gene="SV40 origin"
ORF 1873..2541
/sequence="ORF_1 rf(1)"
misc_binding 1886..1898
/dbxref="REBASE:SfiI"
Marker 1942..2541
/gene="puro marker"
misc_binding 2886..2891
/dbxref="REBASE:BamHI"
Rep_Origin 3110..3729
/gene="pBR322 origin"
Promoter 4382..4431
/gene="NEOKAN prom"
misc_binding 4489..4494
/dbxref="REBASE:BclI"
ORF 4520..5314
/sequence="ORF_2 rf(2)"
Marker 4523..5311
/gene="NTP_II marker"
Rep_Origin 5419..5724
/gene="f1 origin"
BASE COUNT 1359 a 1547 c 1583 g 1309 t 0 others