pAAV-CMV-DIO-GFP-SV40-Puro
| Vector Name | pAAV-CMV-DIO-GFP-SV40-Puro |
| VectorType | AAV Vector |
| Antibiotic Information | Bacterial: Kanamycin Mammalian: Puromycin |
| Sequencing Primers | CMV sequencing primer 5`---CGC AAA TGG GCG GTA GGC GTG---3` |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta acctgcgcgc tcgctcgctc
481 actgaggccg cccgggcaaa gcccgggcgt cgggcgacct ttggtcgccc ggcctcagtg
541 agcgagcgag cgcgcagaga gggagtggcc aactccatca ctaggggttc cttgtagtta
601 atgattaacc cgccatgcta cttatctacg tagccatgct ctaggaagat cgcctaggcg
661 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
721 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
781 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
841 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
901 catgacctta tgggactttc ctacttggca gtacatctac gtttagtcat cgctattacc
961 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
1021 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
1081 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
1141 cggtgggagg tctatataag cagagctggt accaatcagc tcgctcacca tataacttcg
1201 tataaagtat cctatacgaa gttatatcaa aataggaaga ccaatgcttc accatcgacc
1261 cgaattgcca agcatcacca tcgacccata acttcgtata atgtatgcta tacgaagtta
1321 tctcgagcaa gtttgtacaa aaaagcaggc ttcagcgaga tccggtggag ccgggtccgg
1381 cggtgccgtc cacggcagaa ttggacgact gagcgcggga tctggcgaag gcgatggggg
1441 tcttgaaggc gtgctggtac tccacgatgc ccagctcggt gttgctgtgc agctcctcca
1501 cgcggcggaa ggcgaacatg gggcccccgt tctgcaggat gctggggtgg atggcgctct
1561 tgaagtgcat gtggctgtcc accacgaagc tgtagtagcc gccgtcgcgc aggctgaagg
1621 tgcgggcgaa gctgcccacc agcacgttat cgcccatggg gtgcaggtgc tccacggtgg
1681 cgttgctgcg gatgatcttg tcggtgaaga tcacgctgtc ctcggggaag ccggtgccca
1741 ccaccttgaa gtcgccgatc acgcggccgg cctcgtagcg gtagctgaag ctcacgtgca
1801 gcacgccgcc gtcctcgtac ttctcgatgc gggtgttggt gtagccgccg ttgttgatgg
1861 cgtgcaggaa ggggttctcg tagccgctgg ggtaggtgcc gaagtggtag aagccgtagc
1921 ccatcacgtg gctcagcagg taggggctga aggtcagggc gcctttggtg ctcttcatct
1981 tgttggtcat gcggccctgc ttgggggtgc cctctccgcc gcccaccagc tcgaactcca
2041 cgccgttcag ggtgccggtg atgcggcact cgatctccat ggcgggcagg ccgctctcgt
2101 cgctctccat acccagcttt cttgtacaaa gtgggctagc caattgataa cttcgtatag
2161 gatactttat acgaagttat cattgggatt cttcctattt tgatccaagc atcaccatcg
2221 accctctagt ccagatctca ccatcgaccc ataacttcgt atagcataca ttatacgaag
2281 ttatatggtg agcaagggcg attctagaaa cttgtttatt gcagcttata atggttacaa
2341 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
2401 tggtttgtcc aaactcatca atgtatctta aagcttggtg tggaaagtcc ccaggctccc
2461 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
2521 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
2581 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
2641 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
2701 agctattcca gaagtagtga ggaggctttt ttggaggcca tgaccgagta caagcccacg
2761 gtgcgcctcg ccacccgcga cgacgtccct cgggccgtac gcaccctcgc cgccgcgttc
2821 gccgactacc ccgccacgcg ccacaccgtg gacccggacc gccacatcga gcgggtcacc
2881 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
2941 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
3001 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
3061 cagatggaag ggctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
3121 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
3181 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
3241 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
3301 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac tgtgccttct agttgccagc
3361 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
3421 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
3481 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagagaat agcaggcatg
3541 ctggggaaag cttaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc
3601 tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc
3661 tcagtgagcg agcgagcgcg cagggatccc ggtgtgaaat accgcacaga tgcgtaagga
3721 gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
3781 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
3841 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
3901 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
3961 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
4021 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
4081 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
4141 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
4201 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
4261 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
4321 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
4381 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
4441 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
4501 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
4561 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
4621 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
4681 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
4741 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
4801 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
4861 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
4921 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
4981 acgttgttga aaaaggatct tcacctagat ccttttcacg tagaaagcca gtccgcagaa
5041 acggtgctga ccccggatga atgtcagcta ctgggctatc tggacaaggg aaaacgcaag
5101 cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg cgatagctag actgggcggt
5161 tttatggaca gcaagcgaac cggaattgcc agctggggcg ccctctggta aggttgggaa
5221 gccctgcaaa gtaaactgga tggctttctc gccgccaagg atctgatggc gcaggggatc
5281 aagctctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
5341 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
5401 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
5461 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc
5521 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
5581 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
5641 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
5701 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
5761 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
5821 cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca
5881 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
5941 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
6001 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
6061 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gaattttgtt
6121 aaaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aacatccctt
6181 ataaatcaaa agaatagacc gcgatagggt tgagtgttgt tccagtttgg aacaagagtc
6241 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
6301 gcccactacg tgaaccatca cccaaatcaa gttttttgcg gtcgaggtgc cgtaaagctc
6361 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
6421 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
6481 cggtcacgct gcgcgtaacc accacacccg cgcgcttaat gcgccgctac agggcgcgtc
6541 cattcgccat tcaggatcga attaattctt aattaacatc atcaataata tacctt
LOCUS dna 6596 bp
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
misc_binding 457..462
/dbxref="REBASE:HpaI"
misc_binding 457..462
/dbxref="REBASE:HincII"
misc_binding 833..838
/dbxref="REBASE:NdeI"
misc_binding 1168..1173
/dbxref="REBASE:KpnI"
Other Gene 1288..1321
/gene="loxP other"
ORF 1301..2203
/sequence="ORF_2 rf(2)"
misc_binding 1322..1327
/dbxref="REBASE:XhoI"
ORF 1352..2110
/sequence="ORF_4 rf(5)"
misc_binding 1521..1526
/dbxref="REBASE:ApaI"
misc_binding 1532..1537
/dbxref="REBASE:PstI"
misc_binding 2233..2238
/dbxref="REBASE:BglII"
Other Gene 2251..2284
/gene="loxP other"
misc_binding 2303..2308
/dbxref="REBASE:XbaI"
Promoter 2437..2705
/gene="SV40 prom"
Rep_Origin 2604..2681
/gene="SV40 origin"
ORF 2671..3339
/sequence="ORF_1 rf(1)"
misc_binding 2684..2696
/dbxref="REBASE:SfiI"
Marker 2740..3339
/gene="puro marker"
misc_binding 3684..3689
/dbxref="REBASE:BamHI"
Rep_Origin 3908..4527
/gene="pBR322 origin"
Promoter 5180..5229
/gene="NEOKAN prom"
misc_binding 5287..5292
/dbxref="REBASE:BclI"
ORF 5318..6112
/sequence="ORF_3 rf(2)"
Marker 5321..6109
/gene="NTP_II marker"
Rep_Origin 6217..6522
/gene="f1 origin"
BASE COUNT 1482 a 1778 c 1864 g 1472 t 0 others
ORIGIN
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta acctgcgcgc tcgctcgctc
481 actgaggccg cccgggcaaa gcccgggcgt cgggcgacct ttggtcgccc ggcctcagtg
541 agcgagcgag cgcgcagaga gggagtggcc aactccatca ctaggggttc cttgtagtta
601 atgattaacc cgccatgcta cttatctacg tagccatgct ctaggaagat cgcctaggcg
661 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
721 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
781 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
841 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
901 catgacctta tgggactttc ctacttggca gtacatctac gtttagtcat cgctattacc
961 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
1021 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
1081 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
1141 cggtgggagg tctatataag cagagctggt accaatcagc tcgctcacca tataacttcg
1201 tataaagtat cctatacgaa gttatatcaa aataggaaga ccaatgcttc accatcgacc
1261 cgaattgcca agcatcacca tcgacccata acttcgtata atgtatgcta tacgaagtta
1321 tctcgagcaa gtttgtacaa aaaagcaggc ttcagcgaga tccggtggag ccgggtccgg
1381 cggtgccgtc cacggcagaa ttggacgact gagcgcggga tctggcgaag gcgatggggg
1441 tcttgaaggc gtgctggtac tccacgatgc ccagctcggt gttgctgtgc agctcctcca
1501 cgcggcggaa ggcgaacatg gggcccccgt tctgcaggat gctggggtgg atggcgctct
1561 tgaagtgcat gtggctgtcc accacgaagc tgtagtagcc gccgtcgcgc aggctgaagg
1621 tgcgggcgaa gctgcccacc agcacgttat cgcccatggg gtgcaggtgc tccacggtgg
1681 cgttgctgcg gatgatcttg tcggtgaaga tcacgctgtc ctcggggaag ccggtgccca
1741 ccaccttgaa gtcgccgatc acgcggccgg cctcgtagcg gtagctgaag ctcacgtgca
1801 gcacgccgcc gtcctcgtac ttctcgatgc gggtgttggt gtagccgccg ttgttgatgg
1861 cgtgcaggaa ggggttctcg tagccgctgg ggtaggtgcc gaagtggtag aagccgtagc
1921 ccatcacgtg gctcagcagg taggggctga aggtcagggc gcctttggtg ctcttcatct
1981 tgttggtcat gcggccctgc ttgggggtgc cctctccgcc gcccaccagc tcgaactcca
2041 cgccgttcag ggtgccggtg atgcggcact cgatctccat ggcgggcagg ccgctctcgt
2101 cgctctccat acccagcttt cttgtacaaa gtgggctagc caattgataa cttcgtatag
2161 gatactttat acgaagttat cattgggatt cttcctattt tgatccaagc atcaccatcg
2221 accctctagt ccagatctca ccatcgaccc ataacttcgt atagcataca ttatacgaag
2281 ttatatggtg agcaagggcg attctagaaa cttgtttatt gcagcttata atggttacaa
2341 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
2401 tggtttgtcc aaactcatca atgtatctta aagcttggtg tggaaagtcc ccaggctccc
2461 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
2521 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
2581 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
2641 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
2701 agctattcca gaagtagtga ggaggctttt ttggaggcca tgaccgagta caagcccacg
2761 gtgcgcctcg ccacccgcga cgacgtccct cgggccgtac gcaccctcgc cgccgcgttc
2821 gccgactacc ccgccacgcg ccacaccgtg gacccggacc gccacatcga gcgggtcacc
2881 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
2941 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
3001 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
3061 cagatggaag ggctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
3121 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
3181 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
3241 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
3301 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac tgtgccttct agttgccagc
3361 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
3421 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
3481 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagagaat agcaggcatg
3541 ctggggaaag cttaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc
3601 tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc
3661 tcagtgagcg agcgagcgcg cagggatccc ggtgtgaaat accgcacaga tgcgtaagga
3721 gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
3781 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
3841 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
3901 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
3961 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
4021 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
4081 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
4141 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
4201 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
4261 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
4321 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
4381 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
4441 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
4501 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
4561 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
4621 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
4681 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
4741 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
4801 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
4861 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
4921 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
4981 acgttgttga aaaaggatct tcacctagat ccttttcacg tagaaagcca gtccgcagaa
5041 acggtgctga ccccggatga atgtcagcta ctgggctatc tggacaaggg aaaacgcaag
5101 cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg cgatagctag actgggcggt
5161 tttatggaca gcaagcgaac cggaattgcc agctggggcg ccctctggta aggttgggaa
5221 gccctgcaaa gtaaactgga tggctttctc gccgccaagg atctgatggc gcaggggatc
5281 aagctctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
5341 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
5401 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
5461 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc
5521 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
5581 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
5641 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
5701 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
5761 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
5821 cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca
5881 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
5941 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
6001 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
6061 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gaattttgtt
6121 aaaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aacatccctt
6181 ataaatcaaa agaatagacc gcgatagggt tgagtgttgt tccagtttgg aacaagagtc
6241 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
6301 gcccactacg tgaaccatca cccaaatcaa gttttttgcg gtcgaggtgc cgtaaagctc
6361 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
6421 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
6481 cggtcacgct gcgcgtaacc accacacccg cgcgcttaat gcgccgctac agggcgcgtc
6541 cattcgccat tcaggatcga attaattctt aattaacatc atcaataata tacctt
//
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 ttttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgt 60
AAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCA
61 gggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaa 120
CCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTT
Encap other(162,310)>>>
|
121 cacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgccggtgtacacagg 180
GTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCC
181 aagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagt 240
TTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCA
241 aagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttg 300
TTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAAC
301 tgttactcatagcgcgtaatacggcagacctcagcgctagattattgaagcatttatcag 360
ACAATGAGTATCGCGCATTATGCCGTCTGGAGTCGCGATCTAATAACTTCGTAAATAGTC
amp prom(365,393)<<<
|
361 ggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggg 420
CCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCC
HincII
|
HpaI
|
421 gttccgcgcacatttccccgaaaagtgccacctgacgttaacctgcgcgctcgctcgctc 480
CAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAATTGGACGCGCGAGCGAGCGAG
481 actgaggccgcccgggcaaagcccgggcgtcgggcgacctttggtcgcccggcctcagtg 540
TGACTCCGGCGGGCCCGTTTCGGGCCCGCAGCCCGCTGGAAACCAGCGGGCCGGAGTCAC
541 agcgagcgagcgcgcagagagggagtggccaactccatcactaggggttccttgtagtta 600
TCGCTCGCTCGCGCGTCTCTCCCTCACCGGTTGAGGTAGTGATCCCCAAGGAACATCAAT
601 atgattaacccgccatgctacttatctacgtagccatgctctaggaagatcgcctaggcg 660
TACTAATTGGGCGGTACGATGAATAGATGCATCGGTACGAGATCCTTCTAGCGGATCCGC
661 cgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccatt 720
GCAATGTATTGAATGCCATTTACCGGGCGGACCGACTGGCGGGTTGCTGGGGGCGGGTAA
721 gacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtca 780
CTGCAGTTATTACTGCATACAAGGGTATCATTGCGGTTATCCCTGAAAGGTAACTGCAGT
NdeI
|
781 atgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgcc 840
TACCCACCTCATAAATGCCATTTGACGGGTGAACCGTCATGTAGTTCACATAGTATACGG
841 aagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagta 900
TTCATGCGGGGGATAACTGCAGTTACTGCCATTTACCGGGCGGACCGTAATACGGGTCAT
901 catgaccttatgggactttcctacttggcagtacatctacgtttagtcatcgctattacc 960
GTACTGGAATACCCTGAAAGGATGAACCGTCATGTAGATGCAAATCAGTAGCGATAATGG
961 atggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacgggga 1020
TACCACTACGCCAAAACCGTCATGTAGTTACCCGCACCTATCGCCAAACTGAGTGCCCCT
1021 tttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgg 1080
AAAGGTTCAGAGGTGGGGTAACTGCAGTTACCCTCAAACAAAACCGTGGTTTTAGTTGCC
1081 gactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgta 1140
CTGAAAGGTTTTACAGCATTGTTGAGGCGGGGTAACTGCGTTTACCCGCCATCCGCACAT
KpnI
|
1141 cggtgggaggtctatataagcagagctggtaccaatcagctcgctcaccatataacttcg 1200
GCCACCCTCCAGATATATTCGTCTCGACCATGGTTAGTCGAGCGAGTGGTATATTGAAGC
1201 tataaagtatcctatacgaagttatatcaaaataggaagaccaatgcttcaccatcgacc 1260
ATATTTCATAGGATATGCTTCAATATAGTTTTATCCTTCTGGTTACGAAGTGGTAGCTGG
ORF_2 rf(2)(1301,2203)>>>
|
loxP other(1288,1321)<<<
| |
1261 cgaattgccaagcatcaccatcgacccataacttcgtataatgtatgctatacgaagtta 1320
GCTTAACGGTTCGTAGTGGTAGCTGGGTATTGAAGCATATTACATACGATATGCTTCAAT
XhoI ORF_4 rf(5)(1352,2110)<<<
| |
1321 tctcgagCAAGTTTGTACAAAAAAGCAGGCTtcagcgagatccggtggagccgggtccgg 1380
AGAGCTCGTTCAAACATGTTTTTTCGTCCGAAGTCGCTCTAGGCCACCTCGGCCCAGGCC
1381 cggtgccgtccacggcagaattggacgactgagcgcgggatctggcgaaggcgatggggg 1440
GCCACGGCAGGTGCCGTCTTAACCTGCTGACTCGCGCCCTAGACCGCTTCCGCTACCCCC
1441 tcttgaaggcgtgctggtactccacgatgcccagctcggtgttgctgtgcagctcctcca 1500
AGAACTTCCGCACGACCATGAGGTGCTACGGGTCGAGCCACAACGACACGTCGAGGAGGT
ApaI PstI
| |
1501 cgcggcggaaggcgaacatggggcccccgttctgcaggatgctggggtggatggcgctct 1560
GCGCCGCCTTCCGCTTGTACCCCGGGGGCAAGACGTCCTACGACCCCACCTACCGCGAGA
1561 tgaagtgcatgtggctgtccaccacgaagctgtagtagccgccgtcgcgcaggctgaagg 1620
ACTTCACGTACACCGACAGGTGGTGCTTCGACATCATCGGCGGCAGCGCGTCCGACTTCC
1621 tgcgggcgaagctgcccaccagcacgttatcgcccatggggtgcaggtgctccacggtgg 1680
ACGCCCGCTTCGACGGGTGGTCGTGCAATAGCGGGTACCCCACGTCCACGAGGTGCCACC
1681 cgttgctgcggatgatcttgtcggtgaagatcacgctgtcctcggggaagccggtgccca 1740
GCAACGACGCCTACTAGAACAGCCACTTCTAGTGCGACAGGAGCCCCTTCGGCCACGGGT
1741 ccaccttgaagtcgccgatcacgcggccggcctcgtagcggtagctgaagctcacgtgca 1800
GGTGGAACTTCAGCGGCTAGTGCGCCGGCCGGAGCATCGCCATCGACTTCGAGTGCACGT
1801 gcacgccgccgtcctcgtacttctcgatgcgggtgttggtgtagccgccgttgttgatgg 1860
CGTGCGGCGGCAGGAGCATGAAGAGCTACGCCCACAACCACATCGGCGGCAACAACTACC
1861 cgtgcaggaaggggttctcgtagccgctggggtaggtgccgaagtggtagaagccgtagc 1920
GCACGTCCTTCCCCAAGAGCATCGGCGACCCCATCCACGGCTTCACCATCTTCGGCATCG
1921 ccatcacgtggctcagcaggtaggggctgaaggtcagggcgcctttggtgctcttcatct 1980
GGTAGTGCACCGAGTCGTCCATCCCCGACTTCCAGTCCCGCGGAAACCACGAGAAGTAGA
1981 tgttggtcatgcggccctgcttgggggtgccctctccgccgcccaccagctcgaactcca 2040
ACAACCAGTACGCCGGGACGAACCCCCACGGGAGAGGCGGCGGGTGGTCGAGCTTGAGGT
2041 cgccgttcagggtgccggtgatgcggcactcgatctccatggcgggcaggccgctctcgt 2100
GCGGCAAGTCCCACGGCCACTACGCCGTGAGCTAGAGGTACCGCCCGTCCGGCGAGAGCA
2101 cgctctccaTACCCAGCTTTCTTGTACAAAGTGGgctagccaattgataacttcgtatag 2160
GCGAGAGGTATGGGTCGAAAGAACATGTTTCACCCGATCGGTTAACTATTGAAGCATATC
2161 gatactttatacgaagttatcattgggattcttcctattttgatccaagcatcaccatcg 2220
CTATGAAATATGCTTCAATAGTAACCCTAAGAAGGATAAAACTAGGTTCGTAGTGGTAGC
BglII loxP other(2251,2284)>>>
| |
2221 accctctagtccagatctcaccatcgacccataacttcgtatagcatacattatacgaag 2280
TGGGAGATCAGGTCTAGAGTGGTAGCTGGGTATTGAAGCATATCGTATGTAATATGCTTC
XbaI
|
2281 ttatatggtgagcaagggcgattctagaaacttgtttattgcagcttataatggttacaa 2340
AATATACCACTCGTTCCCGCTAAGATCTTTGAACAAATAACGTCGAATATTACCAATGTT
2341 ataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttg 2400
TATTTCGTTATCGTAGTGTTTAAAGTGTTTATTTCGTAAAAAAAGTGACGTAAGATCAAC
SV40 prom(2437,2705)>>>
|
2401 tggtttgtccaaactcatcaatgtatcttaaagcttggtgtggaaagtccccaggctccc 2460
ACCAAACAGGTTTGAGTAGTTACATAGAATTTCGAACCACACCTTTCAGGGGTCCGAGGG
2461 cagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccaggtgtggaaagt 2520
GTCGTCCGTCTTCATACGTTTCGTACGTAGAGTTAATCAGTCGTTGGTCCACACCTTTCA
2521 ccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaacca 2580
GGGGTCCGAGGGGTCGTCCGTCTTCATACGTTTCGTACGTAGAGTTAATCAGTCGTTGGT
SV40 origin(2604,2681)>>>
|
2581 tagtcccgcccctaactccgcccatcccgcccctaactccgcccagttccgcccattctc 2640
ATCAGGGCGGGGATTGAGGCGGGTAGGGCGGGGATTGAGGCGGGTCAAGGCGGGTAAGAG
SfiI
|
ORF_1 rf(1)(2671,3339)>>>
| |
2641 cgccccatggctgactaattttttttatttatgcagaggccgaggccgcctcggcctctg 2700
GCGGGGTACCGACTGATTAAAAAAAATAAATACGTCTCCGGCTCCGGCGGAGCCGGAGAC
puro marker(2740,3339)>>>
|
2701 agctattccagaagtagtgaggaggcttttttggaggccatgaccgagtacaagcccacg 2760
TCGATAAGGTCTTCATCACTCCTCCGAAAAAACCTCCGGTACTGGCTCATGTTCGGGTGC
2761 gtgcgcctcgccacccgcgacgacgtccctcgggccgtacgcaccctcgccgccgcgttc 2820
CACGCGGAGCGGTGGGCGCTGCTGCAGGGAGCCCGGCATGCGTGGGAGCGGCGGCGCAAG
2821 gccgactaccccgccacgcgccacaccgtggacccggaccgccacatcgagcgggtcacc 2880
CGGCTGATGGGGCGGTGCGCGGTGTGGCACCTGGGCCTGGCGGTGTAGCTCGCCCAGTGG
2881 gagctgcaagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcg 2940
CTCGACGTTCTTGAGAAGGAGTGCGCGCAGCCCGAGCTGTAGCCGTTCCACACCCAGCGC
2941 gacgacggcgccgcggtggcggtctggaccacgccggagagcgtcgaagcgggggcggtg 3000
CTGCTGCCGCGGCGCCACCGCCAGACCTGGTGCGGCCTCTCGCAGCTTCGCCCCCGCCAC
3001 ttcgccgagatcggcccgcgcatggccgagttgagcggttcccggctggccgcgcagcaa 3060
AAGCGGCTCTAGCCGGGCGCGTACCGGCTCAACTCGCCAAGGGCCGACCGGCGCGTCGTT
3061 cagatggaagggctcctggcgccgcaccggcccaaggagcccgcgtggttcctggccacc 3120
GTCTACCTTCCCGAGGACCGCGGCGTGGCCGGGTTCCTCGGGCGCACCAAGGACCGGTGG
3121 gtcggcgtctcgcccgaccaccagggcaagggtctgggcagcgccgtcgtgctccccgga 3180
CAGCCGCAGAGCGGGCTGGTGGTCCCGTTCCCAGACCCGTCGCGGCAGCACGAGGGGCCT
3181 gtggaggcggccgagcgcgccggggtgcccgccttcctggagacctccgcgccccgcaac 3240
CACCTCCGCCGGCTCGCGCGGCCCCACGGGCGGAAGGACCTCTGGAGGCGCGGGGCGTTG
3241 ctccccttctacgagcggctcggcttcaccgtcaccgccgacgtcgaggtgcccgaagga 3300
GAGGGGAAGATGCTCGCCGAGCCGAAGTGGCAGTGGCGGCTGCAGCTCCACGGGCTTCCT
3301 ccgcgcacctggtgcatgacccgcaagcccggtgcctgactgtgccttctagttgccagc 3360
GGCGCGTGGACCACGTACTGGGCGTTCGGGCCACGGACTGACACGGAAGATCAACGGTCG
3361 catctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactg 3420
GTAGACAACAAACGGGGAGGGGGCACGGAAGGAACTGGGACCTTCCACGGTGAGGGTGAC
3421 tcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattc 3480
AGGAAAGGATTATTTTACTCCTTTAACGTAGCGTAACAGACTCATCCACAGTAAGATAAG
3481 tggggggtggggtggggcaggacagcaagggggaggattgggaagagaatagcaggcatg 3540
ACCCCCCACCCCACCCCGTCCTGTCGTTCCCCCTCCTAACCCTTCTCTTATCGTCCGTAC
3541 ctggggaaagcttaggaacccctagtgatggagttggccactccctctctgcgcgctcgc 3600
GACCCCTTTCGAATCCTTGGGGATCACTACCTCAACCGGTGAGGGAGAGACGCGCGAGCG
3601 tcgctcactgaggccgggcgaccaaaggtcgcccgacgcccgggctttgcccgggcggcc 3660
AGCGAGTGACTCCGGCCCGCTGGTTTCCAGCGGGCTGCGGGCCCGAAACGGGCCCGCCGG
BamHI
|
3661 tcagtgagcgagcgagcgcgcagggatcccggtgtgaaataccgcacagatgcgtaagga 3720
AGTCACTCGCTCGCTCGCGCGTCCCTAGGGCCACACTTTATGGCGTGTCTACGCATTCCT
3721 gaaaataccgcatcaggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcg 3780
CTTTTATGGCGTAGTCCGCGAGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGAGCCAGC
3781 ttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaat 3840
AAGCCGACGCCGCTCGCCATAGTCGAGTGAGTTTCCGCCATTATGCCAATAGGTGTCTTA
3841 caggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgta 3900
GTCCCCTATTGCGTCCTTTCTTGTACACTCGTTTTCCGGTCGTTTTCCGGTCCTTGGCAT
pBR322 origin(3908,4527)<<<
|
3901 aaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaa 3960
TTTTCCGGCGCAACGACCGCAAAAAGGTATCCGAGGCGGGGGGACTGCTCGTAGTGTTTT
3961 atcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttc 4020
TAGCTGCGAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGATATTTCTATGGTCCGCAAAG
4021 cccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgt 4080
GGGGACCTTCGAGGGAGCACGCGAGAGGACAAGGCTGGGACGGCGAATGGCCTATGGACA
4081 ccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctca 4140
GGCGGAAAGAGGGAAGCCCTTCGCACCGCGAAAGAGTATCGAGTGCGACATCCATAGAGT
4141 gttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccg 4200
CAAGCCACATCCAGCAAGCGAGGTTCGACCCGACACACGTGCTTGGGGGGCAAGTCGGGC
4201 accgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttat 4260
TGGCGACGCGGAATAGGCCATTGATAGCAGAACTCAGGTTGGGCCATTCTGTGCTGAATA
4261 cgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgcta 4320
GCGGTGACCGTCGTCGGTGACCATTGTCCTAATCGTCTCGCTCCATACATCCGCCACGAT
4321 cagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatct 4380
GTCTCAAGAACTTCACCACCGGATTGATGCCGATGTGATCTTCCTGTCATAAACCATAGA
4381 gcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaac 4440
CGCGAGACGACTTCGGTCAATGGAAGCCTTTTTCTCAACCATCGAGAACTAGGCCGTTTG
4441 aaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaa 4500
TTTGGTGGCGACCATCGCCACCAAAAAAACAAACGTTCGTCGTCTAATGCGCGTCTTTTT
4501 aaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaa 4560
TTCCTAGAGTTCTTCTAGGAAACTAGAAAAGATGCCCCAGACTGCGAGTCACCTTGCTTT
4561 actcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttt 4620
TGAGTGCAATTCCCTAAAACCAGTACTCTAATAGTTTTTCCTAGAAGTGGATCTAGGAAA
4621 taaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgaca 4680
ATTTAATTTTTACTTCAAAATTTAGTTAGATTTCATATATACTCATTTGAACCAGACTGT
4681 gttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatcca 4740
CAATGGTTACGAATTAGTCACTCCGTGGATAGAGTCGCTAGACAGATAAAGCAAGTAGGT
4741 tagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggcc 4800
ATCAACGGACTGAGGGGCAGCACATCTATTGATGCTATGCCCTCCCGAATGGTAGACCGG
4801 ccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataa 4860
GGTCACGACGTTACTATGGCGCTCTGGGTGCGAGTGGCCGAGGTCTAAATAGTCGTTATT
4861 accagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatcc 4920
TGGTCGGTCGGCCTTCCCGGCTCGCGTCTTCACCAGGACGTTGAAATAGGCGGAGGTAGG
4921 agtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgca 4980
TCAGATAATTAACAACGGCCCTTCGATCTCATTCATCAAGCGGTCAATTATCAAACGCGT
4981 acgttgttgaaaaaggatcttcacctagatccttttcacgtagaaagccagtccgcagaa 5040
TGCAACAACTTTTTCCTAGAAGTGGATCTAGGAAAAGTGCATCTTTCGGTCAGGCGTCTT
5041 acggtgctgaccccggatgaatgtcagctactgggctatctggacaagggaaaacgcaag 5100
TGCCACGACTGGGGCCTACTTACAGTCGATGACCCGATAGACCTGTTCCCTTTTGCGTTC
5101 cgcaaagagaaagcaggtagcttgcagtgggcttacatggcgatagctagactgggcggt 5160
GCGTTTCTCTTTCGTCCATCGAACGTCACCCGAATGTACCGCTATCGATCTGACCCGCCA
NEOKAN prom(5180,5229)>>>
|
5161 tttatggacagcaagcgaaccggaattgccagctggggcgccctctggtaaggttgggaa 5220
AAATACCTGTCGTTCGCTTGGCCTTAACGGTCGACCCCGCGGGAGACCATTCCAACCCTT
5221 gccctgcaaagtaaactggatggctttctcgccgccaaggatctgatggcgcaggggatc 5280
CGGGACGTTTCATTTGACCTACCGAAAGAGCGGCGGTTCCTAGACTACCGCGTCCCCTAG
NTP_II marker(5321,6109)>>>
|
BclI ORF_3 rf(2)(5318,6112)>>>
| | |
5281 aagctctgatcaagagacaggatgaggatcgtttcgcatgattgaacaagatggattgca 5340
TTCGAGACTAGTTCTCTGTCCTACTCCTAGCAAAGCGTACTAACTTGTTCTACCTAACGT
5341 cgcaggttctccggccgcttgggtggagaggctattcggctatgactgggcacaacagac 5400
GCGTCCAAGAGGCCGGCGAACCCACCTCTCCGATAAGCCGATACTGACCCGTGTTGTCTG
5401 aatcggctgctctgatgccgccgtgttccggctgtcagcgcaggggcgcccggttctttt 5460
TTAGCCGACGAGACTACGGCGGCACAAGGCCGACAGTCGCGTCCCCGCGGGCCAAGAAAA
5461 tgtcaagaccgacctgtccggtgccctgaatgaactgcaagacgaggcagcgcggctatc 5520
ACAGTTCTGGCTGGACAGGCCACGGGACTTACTTGACGTTCTGCTCCGTCGCGCCGATAG
5521 gtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactgaagcggg 5580
CACCGACCGGTGCTGCCCGCAAGGAACGCGTCGACACGAGCTGCAACAGTGACTTCGCCC
5581 aagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcaccttgc 5640
TTCCCTGACCGACGATAACCCGCTTCACGGCCCCGTCCTAGAGGACAGTAGAGTGGAACG
5641 tcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatcc 5700
AGGACGGCTCTTTCATAGGTAGTACCGACTACGTTACGCCGCCGACGTATGCGAACTAGG
5701 ggctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggat 5760
CCGATGGACGGGTAAGCTGGTGGTTCGCTTTGTAGCGTAGCTCGCTCGTGCATGAGCCTA
5761 ggaagccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagc 5820
CCTTCGGCCAGAACAGCTAGTCCTACTAGACCTGCTTCTCGTAGTCCCCGAGCGCGGTCG
5821 cgaactgttcgccaggctcaaggcgagcatgcccgacggcgaggatctcgtcgtgaccca 5880
GCTTGACAAGCGGTCCGAGTTCCGCTCGTACGGGCTGCCGCTCCTAGAGCAGCACTGGGT
5881 tggcgatgcctgcttgccgaatatcatggtggaaaatggccgcttttctggattcatcga 5940
ACCGCTACGGACGAACGGCTTATAGTACCACCTTTTACCGGCGAAAAGACCTAAGTAGCT
5941 ctgtggccggctgggtgtggcggaccgctatcaggacatagcgttggctacccgtgatat 6000
GACACCGGCCGACCCACACCGCCTGGCGATAGTCCTGTATCGCAACCGATGGGCACTATA
6001 tgctgaagagcttggcggcgaatgggctgaccgcttcctcgtgctttacggtatcgccgc 6060
ACGACTTCTCGAACCGCCGCTTACCCGACTGGCGAAGGAGCACGAAATGCCATAGCGGCG
6061 tcccgattcgcagcgcatcgccttctatcgccttcttgacgagttcttctgaattttgtt 6120
AGGGCTAAGCGTCGCGTAGCGGAAGATAGCGGAAGAACTGCTCAAGAAGACTTAAAACAA
6121 aaaatttttgttaaatcagctcattttttaaccaataggccgaaatcggcaacatccctt 6180
TTTTAAAAACAATTTAGTCGAGTAAAAAATTGGTTATCCGGCTTTAGCCGTTGTAGGGAA
f1 origin(6217,6522)<<<
|
6181 ataaatcaaaagaatagaccgcgatagggttgagtgttgttccagtttggaacaagagtc 6240
TATTTAGTTTTCTTATCTGGCGCTATCCCAACTCACAACAAGGTCAAACCTTGTTCTCAG
6241 cactattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcgatg 6300
GTGATAATTTCTTGCACCTGAGGTTGCAGTTTCCCGCTTTTTGGCAGATAGTCCCGCTAC
6301 gcccactacgtgaaccatcacccaaatcaagttttttgcggtcgaggtgccgtaaagctc 6360
CGGGTGATGCACTTGGTAGTGGGTTTAGTTCAAAAAACGCCAGCTCCACGGCATTTCGAG
6361 taaatcggaaccctaaagggagcccccgatttagagcttgacggggaaagccggcgaacg 6420
ATTTAGCCTTGGGATTTCCCTCGGGGGCTAAATCTCGAACTGCCCCTTTCGGCCGCTTGC
6421 tggcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtag 6480
ACCGCTCTTTCCTTCCCTTCTTTCGCTTTCCTCGCCCGCGATCCCGCGACCGTTCACATC
6481 cggtcacgctgcgcgtaaccaccacacccgcgcgcttaatgcgccgctacagggcgcgtc 6540
GCCAGTGCGACGCGCATTGGTGGTGTGGGCGCGCGAATTACGCGGCGATGTCCCGCGCAG
6541 cattcgccattcaggatcgaattaattcttaattaacatcatcaataatatacctt 6596
GTAAGCGGTAAGTCCTAGCTTAATTAAGAATTAATTGTAGTAGTTATTATATGGAA
LOCUS dna 6596 bp
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
misc_binding 457..462
/dbxref="REBASE:HpaI"
misc_binding 457..462
/dbxref="REBASE:HincII"
misc_binding 833..838
/dbxref="REBASE:NdeI"
misc_binding 1168..1173
/dbxref="REBASE:KpnI"
Other Gene 1288..1321
/gene="loxP other"
ORF 1301..2203
/sequence="ORF_2 rf(2)"
misc_binding 1322..1327
/dbxref="REBASE:XhoI"
ORF 1352..2110
/sequence="ORF_4 rf(5)"
misc_binding 1521..1526
/dbxref="REBASE:ApaI"
misc_binding 1532..1537
/dbxref="REBASE:PstI"
misc_binding 2233..2238
/dbxref="REBASE:BglII"
Other Gene 2251..2284
/gene="loxP other"
misc_binding 2303..2308
/dbxref="REBASE:XbaI"
Promoter 2437..2705
/gene="SV40 prom"
Rep_Origin 2604..2681
/gene="SV40 origin"
ORF 2671..3339
/sequence="ORF_1 rf(1)"
misc_binding 2684..2696
/dbxref="REBASE:SfiI"
Marker 2740..3339
/gene="puro marker"
misc_binding 3684..3689
/dbxref="REBASE:BamHI"
Rep_Origin 3908..4527
/gene="pBR322 origin"
Promoter 5180..5229
/gene="NEOKAN prom"
misc_binding 5287..5292
/dbxref="REBASE:BclI"
ORF 5318..6112
/sequence="ORF_3 rf(2)"
Marker 5321..6109
/gene="NTP_II marker"
Rep_Origin 6217..6522
/gene="f1 origin"
BASE COUNT 1482 a 1778 c 1864 g 1472 t 0 others