pAAV-G-CMV-Blank Amp
| Vector Name | pAAV-G-CMV-Blank Amp |
| VectorType | AAV |
| Antibiotic Information | Ampicillin |
| Sequencing Primers | |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtgtct agggttccgc gttacataac ttacggtaaa
181 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
241 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
301 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
361 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
421 tacttggcag tacatctacg tttagtcatc gctattacca tggtgatgcg gttttggcag
481 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
541 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
601 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
661 agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc
721 catagaagaa ccgagtttaa actccctatc agtgatagag atctccctat cagtgataga
781 gagctagcga tatctgactc gagagatcta cgggtggcat ccctgtgacc cctccccagt
841 gcctctcctg gccctggaag ttgccactcc agtgcccacc agccttgtcc taataaaatt
901 aagttgcatc attttgtctg actaggtgtc cttctataat attatggggt ggaggggggt
961 ggtatggagc aaggggcaag ttgggaagac aacctgtagg gcctgcgggg tctattggga
1021 accaagctgg agtgcagtgg cacaatcttg gctcactgca atctccgcct cctgggttca
1081 agcgattctc ctgcctcagc ctcccgagtt gttgggattc caggcatgca tgaccaggct
1141 cagctaattt ttgttttttt ggtagagacg gggtttcacc atattggcca ggctggtctc
1201 caactcctaa tctcaggtga tctacccacc ttggcctccc aaattgctgg gattacaggc
1261 gtgaaccact gctcccttcc ctgtccttct gattttgtag gtaaccacgt gcggaccgag
1321 cggccgcagg aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc
1381 actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg
1441 agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat
1501 ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg
1561 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgcct
1621 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
1681 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
1741 accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
1801 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
1861 gaacaacact caactctatc tcgggctatt cttttgattt ataagggatt ttgccgattt
1921 cggtctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
1981 tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
2041 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
2101 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
2161 caccgtcatc accgaaacgc gcgagccggg agctgcatgt gtcagaggtt ttcaccgtca
2221 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
2281 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
2341 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
2401 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
2461 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
2521 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
2581 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
2641 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
2701 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
2761 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
2821 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
2881 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
2941 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
3001 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
3061 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
3121 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
3181 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
3241 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
3301 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
3361 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
3421 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
3481 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
3541 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
3601 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
3661 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
3721 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
3781 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
3841 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
3901 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
3961 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
4021 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
4081 cggttcctgg ccttttgctg gccttttgct cacatgt
LOCUS dna 4117 bp
FEATURES Location/Qualifiers
misc_binding 332..337
/dbxref="REBASE:NdeI"
misc_binding 458..463
/dbxref="REBASE:NcoI"
Promoter 606..687
/gene="CMV prom"
misc_binding 662..667
/dbxref="REBASE:SacI"
Regulatory_Seq 743..782
/gene="tetO reg"
misc_binding 789..794
/dbxref="REBASE:EcoRV"
misc_binding 798..803
/dbxref="REBASE:XhoI"
Terminator 810..1288
/gene="hGH_PA term"
misc_binding 1124..1129
/dbxref="REBASE:SphI"
misc_binding 1469..1474
/dbxref="REBASE:NarI"
Rep_Origin 1560..1866
/gene="f1 origin"
Promoter 2367..2395
/gene="amp prom"
Marker 2437..3297
/gene="amp marker"
ORF 2437..3297
/sequence="ORF_1 rf(1)"
Rep_Origin 3452..4071
/gene="pBR322 origin"
misc_binding 3698..3706
/dbxref="REBASE:AlwNI"
BASE COUNT 946 a 1069 c 1053 g 1049 t 0 others
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtgtct agggttccgc gttacataac ttacggtaaa
181 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
241 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
301 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
361 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
421 tacttggcag tacatctacg tttagtcatc gctattacca tggtgatgcg gttttggcag
481 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
541 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
601 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
661 agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc
721 catagaagaa ccgagtttaa actccctatc agtgatagag atctccctat cagtgataga
781 gagctagcga tatctgactc gagagatcta cgggtggcat ccctgtgacc cctccccagt
841 gcctctcctg gccctggaag ttgccactcc agtgcccacc agccttgtcc taataaaatt
901 aagttgcatc attttgtctg actaggtgtc cttctataat attatggggt ggaggggggt
961 ggtatggagc aaggggcaag ttgggaagac aacctgtagg gcctgcgggg tctattggga
1021 accaagctgg agtgcagtgg cacaatcttg gctcactgca atctccgcct cctgggttca
1081 agcgattctc ctgcctcagc ctcccgagtt gttgggattc caggcatgca tgaccaggct
1141 cagctaattt ttgttttttt ggtagagacg gggtttcacc atattggcca ggctggtctc
1201 caactcctaa tctcaggtga tctacccacc ttggcctccc aaattgctgg gattacaggc
1261 gtgaaccact gctcccttcc ctgtccttct gattttgtag gtaaccacgt gcggaccgag
1321 cggccgcagg aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc
1381 actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg
1441 agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat
1501 ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg
1561 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgcct
1621 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
1681 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
1741 accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
1801 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
1861 gaacaacact caactctatc tcgggctatt cttttgattt ataagggatt ttgccgattt
1921 cggtctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
1981 tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
2041 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
2101 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
2161 caccgtcatc accgaaacgc gcgagccggg agctgcatgt gtcagaggtt ttcaccgtca
2221 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
2281 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
2341 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
2401 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
2461 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
2521 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
2581 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
2641 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
2701 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
2761 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
2821 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
2881 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
2941 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
3001 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
3061 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
3121 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
3181 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
3241 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
3301 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
3361 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
3421 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
3481 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
3541 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
3601 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
3661 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
3721 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
3781 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
3841 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
3901 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
3961 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
4021 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
4081 cggttcctgg ccttttgctg gccttttgct cacatgt
//
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 cctgcaggcagctgcgcgctcgctcgctcactgaggccgcccgggcgtcgggcgaccttt 60
GGACGTCCGTCGACGCGCGAGCGAGCGAGTGACTCCGGCGGGCCCGCAGCCCGCTGGAAA
61 ggtcgcccggcctcagtgagcgagcgagcgcgcagagagggagtggccaactccatcact 120
CCAGCGGGCCGGAGTCACTCGCTCGCTCGCGCGTCTCTCCCTCACCGGTTGAGGTAGTGA
121 aggggttcctgcggccgcacgcgtgtgtctagggttccgcgttacataacttacggtaaa 180
TCCCCAAGGACGCCGGCGTGCGCACACAGATCCCAAGGCGCAATGTATTGAATGCCATTT
181 tggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgt 240
ACCGGGCGGACCGACTGGCGGGTTGCTGGGGGCGGGTAACTGCAGTTATTACTGCATACA
241 tcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggta 300
AGGGTATCATTGCGGTTATCCCTGAAAGGTAACTGCAGTTACCCACCTCATAAATGCCAT
NdeI
|
301 aactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgt 360
TTGACGGGTGAACCGTCATGTAGTTCACATAGTATACGGTTCATGCGGGGGATAACTGCA
361 caatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcc 420
GTTACTGCCATTTACCGGGCGGACCGTAATACGGGTCATGTACTGGAATACCCTGAAAGG
NcoI
|
421 tacttggcagtacatctacgtttagtcatcgctattaccatggtgatgcggttttggcag 480
ATGAACCGTCATGTAGATGCAAATCAGTAGCGATAATGGTACCACTACGCCAAAACCGTC
481 tacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccatt 540
ATGTAGTTACCCGCACCTATCGCCAAACTGAGTGCCCCTAAAGGTTCAGAGGTGGGGTAA
541 gacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaac 600
CTGCAGTTACCCTCAAACAAAACCGTGGTTTTAGTTGCCCTGAAAGGTTTTACAGCATTG
CMV prom(606,687)>>>
|
601 aactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagc 660
TTGAGGCGGGGTAACTGCGTTTACCCGCCATCCGCACATGCCACCCTCCAGATATATTCG
SacI
|
661 agagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctc 720
TCTCGAGCAAATCACTTGGCAGTCTAGCGGACCTCTGCGGTAGGTGCGACAAAACTGGAG
tetO reg(743,782)>>>
|
721 catagaagaaccgagtttaaactccctatcagtgatagagatctccctatcagtgataga 780
GTATCTTCTTGGCTCAAATTTGAGGGATAGTCACTATCTCTAGAGGGATAGTCACTATCT
EcoRV XhoI hGH_PA term(810,1288)>>>
| | |
781 gagctagcgatatctgactcgagagatctacgggtggcatccctgtgacccctccccagt 840
CTCGATCGCTATAGACTGAGCTCTCTAGATGCCCACCGTAGGGACACTGGGGAGGGGTCA
841 gcctctcctggccctggaagttgccactccagtgcccaccagccttgtcctaataaaatt 900
CGGAGAGGACCGGGACCTTCAACGGTGAGGTCACGGGTGGTCGGAACAGGATTATTTTAA
901 aagttgcatcattttgtctgactaggtgtccttctataatattatggggtggaggggggt 960
TTCAACGTAGTAAAACAGACTGATCCACAGGAAGATATTATAATACCCCACCTCCCCCCA
961 ggtatggagcaaggggcaagttgggaagacaacctgtagggcctgcggggtctattggga 1020
CCATACCTCGTTCCCCGTTCAACCCTTCTGTTGGACATCCCGGACGCCCCAGATAACCCT
1021 accaagctggagtgcagtggcacaatcttggctcactgcaatctccgcctcctgggttca 1080
TGGTTCGACCTCACGTCACCGTGTTAGAACCGAGTGACGTTAGAGGCGGAGGACCCAAGT
SphI
|
1081 agcgattctcctgcctcagcctcccgagttgttgggattccaggcatgcatgaccaggct 1140
TCGCTAAGAGGACGGAGTCGGAGGGCTCAACAACCCTAAGGTCCGTACGTACTGGTCCGA
1141 cagctaatttttgtttttttggtagagacggggtttcaccatattggccaggctggtctc 1200
GTCGATTAAAAACAAAAAAACCATCTCTGCCCCAAAGTGGTATAACCGGTCCGACCAGAG
1201 caactcctaatctcaggtgatctacccaccttggcctcccaaattgctgggattacaggc 1260
GTTGAGGATTAGAGTCCACTAGATGGGTGGAACCGGAGGGTTTAACGACCCTAATGTCCG
1261 gtgaaccactgctcccttccctgtccttctgattttgtaggtaaccacgtgcggaccgag 1320
CACTTGGTGACGAGGGAAGGGACAGGAAGACTAAAACATCCATTGGTGCACGCCTGGCTC
1321 cggccgcaggaacccctagtgatggagttggccactccctctctgcgcgctcgctcgctc 1380
GCCGGCGTCCTTGGGGATCACTACCTCAACCGGTGAGGGAGAGACGCGCGAGCGAGCGAG
1381 actgaggccgggcgaccaaaggtcgcccgacgcccgggctttgcccgggcggcctcagtg 1440
TGACTCCGGCCCGCTGGTTTCCAGCGGGCTGCGGGCCCGAAACGGGCCCGCCGGAGTCAC
NarI
|
1441 agcgagcgagcgcgcagctgcctgcaggggcgcctgatgcggtattttctccttacgcat 1500
TCGCTCGCTCGCGCGTCGACGGACGTCCCCGCGGACTACGCCATAAAAGAGGAATGCGTA
1501 ctgtgcggtatttcacaccgcatacgtcaaagcaaccatagtacgcgccctgtagcggcg 1560
GACACGCCATAAAGTGTGGCGTATGCAGTTTCGTTGGTATCATGCGCGGGACATCGCCGC
f1 origin(1560,1866)>>>
|
1561 cattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgcct 1620
GTAATTCGCGCCGCCCACACCACCAATGCGCGTCGCACTGGCGATGTGAACGGTCGCGGA
1621 tagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttcccc 1680
ATCGCGGGCGAGGAAAGCGAAAGAAGGGAAGGAAAGAGCGGTGCAAGCGGCCGAAAGGGG
1681 gtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcg 1740
CAGTTCGAGATTTAGCCCCCGAGGGAAATCCCAAGGCTAAATCACGAAATGCCGTGGAGC
1741 accccaaaaaacttgatttgggtgatggttcacgtagtgggccatcgccctgatagacgg 1800
TGGGGTTTTTTGAACTAAACCCACTACCAAGTGCATCACCCGGTAGCGGGACTATCTGCC
1801 tttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactg 1860
AAAAAGCGGGAAACTGCAACCTCAGGTGCAAGAAATTATCACCTGAGAACAAGGTTTGAC
1861 gaacaacactcaactctatctcgggctattcttttgatttataagggattttgccgattt 1920
CTTGTTGTGAGTTGAGATAGAGCCCGATAAGAAAACTAAATATTCCCTAAAACGGCTAAA
1921 cggtctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaa 1980
GCCAGATAACCAATTTTTTACTCGACTAAATTGTTTTTAAATTGCGCTTAAAATTGTTTT
1981 tattaacgtttacaattttatggtgcactctcagtacaatctgctctgatgccgcatagt 2040
ATAATTGCAAATGTTAAAATACCACGTGAGAGTCATGTTAGACGAGACTACGGCGTATCA
2041 taagccagccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcc 2100
ATTCGGTCGGGGCTGTGGGCGGTTGTGGGCGACTGCGCGGGACTGCCCGAACAGACGAGG
2101 cggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggtttt 2160
GCCGTAGGCGAATGTCTGTTCGACACTGGCAGAGGCCCTCGACGTACACAGTCTCCAAAA
2161 caccgtcatcaccgaaacgcgcgagccgggagctgcatgtgtcagaggttttcaccgtca 2220
GTGGCAGTAGTGGCTTTGCGCGCTCGGCCCTCGACGTACACAGTCTCCAAAAGTGGCAGT
2221 tcaccgaaacgcgcgagacgaaagggcctcgtgatacgcctatttttataggttaatgtc 2280
AGTGGCTTTGCGCGCTCTGCTTTCCCGGAGCACTATGCGGATAAAAATATCCAATTACAG
2281 atgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacc 2340
TACTATTATTACCAAAGAATCTGCAGTCCACCGTGAAAAGCCCCTTTACACGCGCCTTGG
amp prom(2367,2395)>>>
|
2341 cctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccc 2400
GGATAAACAAATAAAAAGATTTATGTAAGTTTATACATAGGCGAGTACTCTGTTATTGGG
ORF_1 rf(1)(2437,3297)>>>
|
amp marker(2437,3297)>>>
|
2401 tgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtc 2460
ACTATTTACGAAGTTATTATAACTTTTTCCTTCTCATACTCATAAGTTGTAAAGGCACAG
2461 gcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctg 2520
CGGGAATAAGGGAAAAAACGCCGTAAAACGGAAGGACAAAAACGAGTGGGTCTTTGCGAC
2521 gtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggat 2580
CACTTTCATTTTCTACGACTTCTAGTCAACCCACGTGCTCACCCAATGTAGCTTGACCTA
2581 ctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagc 2640
GAGTTGTCGCCATTCTAGGAACTCTCAAAAGCGGGGCTTCTTGCAAAAGGTTACTACTCG
2641 acttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaa 2700
TGAAAATTTCAAGACGATACACCGCGCCATAATAGGGCATAACTGCGGCCCGTTCTCGTT
2701 ctcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaa 2760
GAGCCAGCGGCGTATGTGATAAGAGTCTTACTGAACCAACTCATGAGTGGTCAGTGTCTT
2761 aagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagt 2820
TTCGTAGAATGCCTACCGTACTGTCATTCTCTTAATACGTCACGACGGTATTGGTACTCA
2821 gataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgct 2880
CTATTGTGACGCCGGTTGAATGAAGACTGTTGCTAGCCTCCTGGCTTCCTCGATTGGCGA
2881 tttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaat 2940
AAAAACGTGTTGTACCCCCTAGTACATTGAGCGGAACTAGCAACCCTTGGCCTCGACTTA
2941 gaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttg 3000
CTTCGGTATGGTTTGCTGCTCGCACTGTGGTGCTACGGACATCGTTACCGTTGTTGCAAC
3001 cgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactgg 3060
GCGTTTGATAATTGACCGCTTGATGAATGAGATCGAAGGGCCGTTGTTAATTATCTGACC
3061 atggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggttt 3120
TACCTCCGCCTATTTCAACGTCCTGGTGAAGACGCGAGCCGGGAAGGCCGACCGACCAAA
3121 attgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactgggg 3180
TAACGACTATTTAGACCTCGGCCACTCGCACCCAGAGCGCCATAGTAACGTCGTGACCCC
3181 ccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatg 3240
GGTCTACCATTCGGGAGGGCATAGCATCAATAGATGTGCTGCCCCTCAGTCCGTTGATAC
3241 gatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactg 3300
CTACTTGCTTTATCTGTCTAGCGACTCTATCCACGGAGTGACTAATTCGTAACCATTGAC
3301 tcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaa 3360
AGTCTGGTTCAAATGAGTATATATGAAATCTAACTAAATTTTGAAGTAAAAATTAAATTT
3361 aggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttt 3420
TCCTAGATCCACTTCTAGGAAAAACTATTAGAGTACTGGTTTTAGGGAATTGCACTCAAA
pBR322 origin(3452,4071)>>>
|
3421 tcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttt 3480
AGCAAGGTGACTCGCAGTCTGGGGCATCTTTTCTAGTTTCCTAGAAGAACTCTAGGAAAA
3481 tttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgt 3540
AAAGACGCGCATTAGACGACGAACGTTTGTTTTTTTGGTGGCGATGGTCGCCACCAAACA
3541 ttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcag 3600
AACGGCCTAGTTCTCGATGGTTGAGAAAAAGGCTTCCATTGACCGAAGTCGTCTCGCGTC
3601 ataccaaatactgttcttctagtgtagccgtagttaggccaccacttcaagaactctgta 3660
TATGGTTTATGACAAGAAGATCACATCGGCATCAATCCGGTGGTGAAGTTCTTGAGACAT
AlwNI
|
3661 gcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgat 3720
CGTGGCGGATGTATGGAGCGAGACGATTAGGACAATGGTCACCGACGACGGTCACCGCTA
3721 aagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcg 3780
TTCAGCACAGAATGGCCCAACCTGAGTTCTGCTATCAATGGCCTATTCCGCGTCGCCAGC
3781 ggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactg 3840
CCGACTTGCCCCCCAAGCACGTGTGTCGGGTCGAACCTCGCTTGCTGGATGTGGCTTGAC
3841 agatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggac 3900
TCTATGGATGTCGCACTCGATACTCTTTCGCGGTGCGAAGGGCTTCCCTCTTTCCGCCTG
3901 aggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccaggggga 3960
TCCATAGGCCATTCGCCGTCCCAGCCTTGTCCTCTCGCGTGCTCCCTCGAAGGTCCCCCT
3961 aacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgattt 4020
TTGCGGACCATAGAAATATCAGGACAGCCCAAAGCGGTGGAGACTGAACTCGCAGCTAAA
4021 ttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggccttttta 4080
AACACTACGAGCAGTCCCCCCGCCTCGGATACCTTTTTGCGGTCGTTGCGCCGGAAAAAT
4081 cggttcctggccttttgctggccttttgctcacatgt 4117
GCCAAGGACCGGAAAACGACCGGAAAACGAGTGTACA
LOCUS dna 4117 bp
FEATURES Location/Qualifiers
misc_binding 332..337
/dbxref="REBASE:NdeI"
misc_binding 458..463
/dbxref="REBASE:NcoI"
Promoter 606..687
/gene="CMV prom"
misc_binding 662..667
/dbxref="REBASE:SacI"
Regulatory_Seq 743..782
/gene="tetO reg"
misc_binding 789..794
/dbxref="REBASE:EcoRV"
misc_binding 798..803
/dbxref="REBASE:XhoI"
Terminator 810..1288
/gene="hGH_PA term"
misc_binding 1124..1129
/dbxref="REBASE:SphI"
misc_binding 1469..1474
/dbxref="REBASE:NarI"
Rep_Origin 1560..1866
/gene="f1 origin"
Promoter 2367..2395
/gene="amp prom"
Marker 2437..3297
/gene="amp marker"
ORF 2437..3297
/sequence="ORF_1 rf(1)"
Rep_Origin 3452..4071
/gene="pBR322 origin"
misc_binding 3698..3706
/dbxref="REBASE:AlwNI"
BASE COUNT 946 a 1069 c 1053 g 1049 t 0 others