pAAV-G-CMV-CBH-gcGFP Amp
| Vector Name | pAAV-G-CMV-CBH-gcGFP Amp |
| VectorType | AAV |
| Antibiotic Information | Ampicillin |
| Sequencing Primers | |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgccc taggcgttac ataacttacg gtaaatggcc cgcctggctg
181 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
241 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
301 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
361 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
421 ctacgtttag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
481 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
541 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
601 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg
661 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagaaccgag
721 tttaaactcc ctatcagtga tagagatctc cctatcagtg atagagagct agcgatatct
781 gactcgagta taaggatgat gacgacaaat gagctagcac ataacttacg gtaaatggcc
841 cgcctggctg accgcccaac gacccccgcc cattgacgtc aatagtaacg ccaataggga
901 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
961 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
1021 ggcattgtgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
1081 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
1141 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
1201 tgggggcggg gggggggggg gcgcgcgcca ggcggggcgg ggcggggcga ggggcggggc
1261 ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg gcgcgctcca aaagtttcct
1321 tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga
1381 gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc
1441 cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg
1501 ggctgtaatt agcttcgaac gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg
1561 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
1621 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
1681 gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt gacctacggc gtgcagtgct
1741 tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
1801 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
1861 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
1921 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaaggtct
1981 atatcaccgc cgacaagcag aagaacggca tcaaggtgaa cttcaagacc cgccacaaca
2041 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
2101 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc
2161 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
2221 tcggcatgga cgagctgtac aagtaactta aggggtggca tccctgtgac ccctccccag
2281 tgcctctcct ggccctggaa gttgccactc cagtgcccac cagccttgtc ctaataaaat
2341 taagttgcat cattttgtct gactaggtgt ccttctataa tattatgggg tggagggggg
2401 tggtatggag caaggggcaa gttgggaaga caacctgtag ggcctgcggg gtctattggg
2461 aaccaagctg gagtgcagtg gcacaatctt ggctcactgc aatctccgcc tcctgggttc
2521 aagcgattct cctgcctcag cctcccgagt tgttgggatt ccaggcatgc atgaccaggc
2581 tcagctaatt tttgtttttt tggtagagac ggggtttcac catattggcc aggctggtct
2641 ccaactccta atctcaggtg atctacccac cttggcctcc caaattgctg ggattacagg
2701 cgtgaaccac tgctcccttc cctgtccttc acgtgcggac cgagcggccg caggaacccc
2761 tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac
2821 caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca
2881 gctgcctgca ggggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
2941 accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg
3001 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccttagcgc ccgctccttt
3061 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
3121 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
3181 tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
3241 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaactc
3301 tatctcgggc tattcttttg atttataagg gattttgccg atttcggtct attggttaaa
3361 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat
3421 tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
3481 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
3541 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
3601 acgcgcgagc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
3661 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
3721 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
3781 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
3841 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
3901 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
3961 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
4021 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
4081 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
4141 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
4201 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
4261 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
4321 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
4381 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
4441 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
4501 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
4561 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
4621 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
4681 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
4741 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
4801 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
4861 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
4921 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
4981 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
5041 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
5101 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
5161 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
5221 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
5281 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
5341 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
5401 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
5461 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
5521 gctggccttt tgctcacatg t
LOCUS dna 5541 bp
FEATURES Location/Qualifiers
Promoter 591..672
/gene="CMV prom"
misc_binding 647..652
/dbxref="REBASE:SacI"
Regulatory_Seq 728..767
/gene="tetO reg"
misc_binding 744..749
/dbxref="REBASE:BglII"
misc_binding 774..779
/dbxref="REBASE:EcoRV"
misc_binding 783..788
/dbxref="REBASE:XhoI"
Promoter 1099..1378
/gene="cBA prom"
ORF 1509..2261
/sequence="ORF_3 rf(5)"
Reporter 1527..2243
/gene="EGFP reporter"
ORF 1527..2246
/sequence="ORF_1 rf(3)"
Terminator 2253..2729
/gene="hGH_PA term"
misc_binding 2565..2570
/dbxref="REBASE:SphI"
misc_binding 2893..2898
/dbxref="REBASE:NarI"
Rep_Origin 2984..3290
/gene="f1 origin"
Promoter 3791..3819
/gene="amp prom"
Marker 3861..4721
/gene="amp marker"
ORF 3861..4721
/sequence="ORF_2 rf(3)"
Rep_Origin 4876..5495
/gene="pBR322 origin"
misc_binding 5122..5130
/dbxref="REBASE:AlwNI"
BASE COUNT 1250 a 1532 c 1468 g 1291 t 0 others
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgccc taggcgttac ataacttacg gtaaatggcc cgcctggctg
181 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
241 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
301 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
361 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
421 ctacgtttag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
481 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
541 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
601 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg
661 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagaaccgag
721 tttaaactcc ctatcagtga tagagatctc cctatcagtg atagagagct agcgatatct
781 gactcgagta taaggatgat gacgacaaat gagctagcac ataacttacg gtaaatggcc
841 cgcctggctg accgcccaac gacccccgcc cattgacgtc aatagtaacg ccaataggga
901 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
961 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
1021 ggcattgtgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
1081 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
1141 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
1201 tgggggcggg gggggggggg gcgcgcgcca ggcggggcgg ggcggggcga ggggcggggc
1261 ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg gcgcgctcca aaagtttcct
1321 tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga
1381 gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc
1441 cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg
1501 ggctgtaatt agcttcgaac gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg
1561 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
1621 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
1681 gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt gacctacggc gtgcagtgct
1741 tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
1801 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
1861 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
1921 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaaggtct
1981 atatcaccgc cgacaagcag aagaacggca tcaaggtgaa cttcaagacc cgccacaaca
2041 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
2101 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc
2161 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
2221 tcggcatgga cgagctgtac aagtaactta aggggtggca tccctgtgac ccctccccag
2281 tgcctctcct ggccctggaa gttgccactc cagtgcccac cagccttgtc ctaataaaat
2341 taagttgcat cattttgtct gactaggtgt ccttctataa tattatgggg tggagggggg
2401 tggtatggag caaggggcaa gttgggaaga caacctgtag ggcctgcggg gtctattggg
2461 aaccaagctg gagtgcagtg gcacaatctt ggctcactgc aatctccgcc tcctgggttc
2521 aagcgattct cctgcctcag cctcccgagt tgttgggatt ccaggcatgc atgaccaggc
2581 tcagctaatt tttgtttttt tggtagagac ggggtttcac catattggcc aggctggtct
2641 ccaactccta atctcaggtg atctacccac cttggcctcc caaattgctg ggattacagg
2701 cgtgaaccac tgctcccttc cctgtccttc acgtgcggac cgagcggccg caggaacccc
2761 tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac
2821 caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca
2881 gctgcctgca ggggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
2941 accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg
3001 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccttagcgc ccgctccttt
3061 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
3121 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
3181 tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
3241 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaactc
3301 tatctcgggc tattcttttg atttataagg gattttgccg atttcggtct attggttaaa
3361 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat
3421 tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
3481 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
3541 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
3601 acgcgcgagc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
3661 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
3721 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
3781 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
3841 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
3901 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
3961 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
4021 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
4081 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
4141 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
4201 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
4261 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
4321 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
4381 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
4441 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
4501 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
4561 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
4621 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
4681 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
4741 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
4801 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
4861 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
4921 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
4981 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
5041 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
5101 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
5161 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
5221 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
5281 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
5341 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
5401 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
5461 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
5521 gctggccttt tgctcacatg t
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 cctgcaggcagctgcgcgctcgctcgctcactgaggccgcccgggcgtcgggcgaccttt 60
GGACGTCCGTCGACGCGCGAGCGAGCGAGTGACTCCGGCGGGCCCGCAGCCCGCTGGAAA
61 ggtcgcccggcctcagtgagcgagcgagcgcgcagagagggagtggccaactccatcact 120
CCAGCGGGCCGGAGTCACTCGCTCGCTCGCGCGTCTCTCCCTCACCGGTTGAGGTAGTGA
121 aggggttcctgcggccgcCCTAGGcgttacataacttacggtaaatggcccgcctggctg 180
TCCCCAAGGACGCCGGCGGGATCCGCAATGTATTGAATGCCATTTACCGGGCGGACCGAC
181 accgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgcc 240
TGGCGGGTTGCTGGGGGCGGGTAACTGCAGTTATTACTGCATACAAGGGTATCATTGCGG
241 aatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggc 300
TTATCCCTGAAAGGTAACTGCAGTTACCCACCTCATAAATGCCATTTGACGGGTGAACCG
301 agtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatg 360
TCATGTAGTTCACATAGTATACGGTTCATGCGGGGGATAACTGCAGTTACTGCCATTTAC
361 gcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacat 420
CGGGCGGACCGTAATACGGGTCATGTACTGGAATACCCTGAAAGGATGAACCGTCATGTA
421 ctacgtttagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgt 480
GATGCAAATCAGTAGCGATAATGGTACCACTACGCCAAAACCGTCATGTAGTTACCCGCA
481 ggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagt 540
CCTATCGCCAAACTGAGTGCCCCTAAAGGTTCAGAGGTGGGGTAACTGCAGTTACCCTCA
CMV prom(591,672)>>>
|
541 ttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattg 600
AACAAAACCGTGGTTTTAGTTGCCCTGAAAGGTTTTACAGCATTGTTGAGGCGGGGTAAC
SacI
|
601 acgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctcgtttagtg 660
TGCGTTTACCCGCCATCCGCACATGCCACCCTCCAGATATATTCGTCTCGAGCAAATCAC
661 aaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagaagaaccgag 720
TTGGCAGTCTAGCGGACCTCTGCGGTAGGTGCGACAAAACTGGAGGTATCTTCTTGGCTC
BglII
|
tetO reg(728,767)>>> EcoRV
| | |
721 tttaaactccctatcagtgatagagatctccctatcagtgatagagagctagcgatatct 780
AAATTTGAGGGATAGTCACTATCTCTAGAGGGATAGTCACTATCTCTCGATCGCTATAGA
XhoI
|
781 gactcgagTATAAGGATGATGACGACAAATGAGCTAGCACATAACTTACGGTAAATGGCC 840
CTGAGCTCATATTCCTACTACTGCTGTTTACTCGATCGTGTATTGAATGCCATTTACCGG
841 CGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAGTAACGCCAATAGGGA 900
GCGGACCGACTGGCGGGTTGCTGGGGGCGGGTAACTGCAGTTATCATTGCGGTTATCCCT
901 CTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATC 960
GAAAGGTAACTGCAGTTACCCACCTCATAAATGCCATTTGACGGGTGAACCGTCATGTAG
961 AAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCT 1020
TTCACATAGTATACGGTTCATGCGGGGGATAACTGCAGTTACTGCCATTTACCGGGCGGA
1021 GGCATTGTGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTAT 1080
CCGTAACACGGGTCATGTACTGGAATACCCTGAAAGGATGAACCGTCATGTAGATGCATA
cBA prom(1099,1378)>>>
|
1081 TAGTCATCGCTATTACCATGGTCGAGGTGAGCCCCACGTTCTGCTTCACTCTCCCCATCT 1140
ATCAGTAGCGATAATGGTACCAGCTCCACTCGGGGTGCAAGACGAAGTGAGAGGGGTAGA
1141 CCCCCCCCTCCCCACCCCCAATTTTGTATTTATTTATTTTTTAATTATTTTGTGCAGCGA 1200
GGGGGGGGAGGGGTGGGGGTTAAAACATAAATAAATAAAAAATTAATAAAACACGTCGCT
1201 TGGGGGCGGGGGGGGGGGGGGCGCGCGCCAGGCGGGGCGGGGCGGGGCGAGGGGCGGGGC 1260
ACCCCCGCCCCCCCCCCCCCCGCGCGCGGTCCGCCCCGCCCCGCCCCGCTCCCCGCCCCG
1261 GGGGCGAGGCGGAGAGGTGCGGCGGCAGCCAATCAGAGCGGCGCGCTCCAAAAGTTTCCT 1320
CCCCGCTCCGCCTCTCCACGCCGCCGTCGGTTAGTCTCGCCGCGCGAGGTTTTCAAAGGA
1321 TTTATGGCGAGGCGGCGGCGGCGGCGGCCCTATAAAAAGCGAAGCGCGCGGCGGGCGGGA 1380
AAATACCGCTCCGCCGCCGCCGCCGCCGGGATATTTTTCGCTTCGCGCGCCGCCCGCCCT
1381 GTCGCTGCGCGCTGCCTTCGCCCCGTGCCCCGCTCCGCCGCCGCCTCGCGCCGCCCGCCC 1440
CAGCGACGCGCGACGGAAGCGGGGCACGGGGCGAGGCGGCGGCGGAGCGCGGCGGGCGGG
1441 CGGCTCTGACTGACCGCGTTACTCCCACAGGTGAGCGGGCGGGACGGCCCTTCTCCTCCG 1500
GCCGAGACTGACTGGCGCAATGAGGGTGTCCACTCGCCCGCCCTGCCGGGAAGAGGAGGC
EGFP reporter(1527,2243)>>>
|
ORF_1 rf(3)(1527,2246)>>>
|
ORF_3 rf(5)(1509,2261)<<<
| |
1501 GGCTGTAATTAGCTTCGAACGCCACCATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGG 1560
CCGACATTAATCGAAGCTTGCGGTGGTACCACTCGTTCCCGCTCCTCGACAAGTGGCCCC
1561 TGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCG 1620
ACCACGGGTAGGACCAGCTCGACCTGCCGCTGCATTTGCCGGTGTTCAAGTCGCACAGGC
1621 GCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCG 1680
CGCTCCCGCTCCCGCTACGGTGGATGCCGTTCGACTGGGACTTCAAGTAGACGTGGTGGC
1681 GCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCTTGACCTACGGCGTGCAGTGCT 1740
CGTTCGACGGGCACGGGACCGGGTGGGAGCACTGGTGGAACTGGATGCCGCACGTCACGA
1741 TCGCCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAG 1800
AGCGGGCGATGGGGCTGGTGTACTTCGTCGTGCTGAAGAAGTTCAGGCGGTACGGGCTTC
1801 GCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCG 1860
CGATGCAGGTCCTCGCGTGGTAGAAGAAGTTCCTGCTGCCGTTGATGTTCTGGGCGCGGC
1861 AGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCA 1920
TCCACTTCAAGCTCCCGCTGTGGGACCACTTGGCGTAGCTCGACTTCCCGTAGCTGAAGT
1921 AGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAAGGTCT 1980
TCCTCCTGCCGTTGTAGGACCCCGTGTTCGACCTCATGTTGATGTTGTCGGTGTTCCAGA
1981 ATATCACCGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGACCCGCCACAACA 2040
TATAGTGGCGGCTGTTCGTCTTCTTGCCGTAGTTCCACTTGAAGTTCTGGGCGGTGTTGT
2041 TCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACG 2100
AGCTCCTGCCGTCGCACGTCGAGCGGCTGGTGATGGTCGTCTTGTGGGGGTAGCCGCTGC
2101 GCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACC 2160
CGGGGCACGACGACGGGCTGTTGGTGATGGACTCGTGGGTCAGGCGGGACTCGTTTCTGG
2161 CCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTC 2220
GGTTGCTCTTCGCGCTAGTGTACCAGGACGACCTCAAGCACTGGCGGCGGCCCTAGTGAG
hGH_PA term(2253,2729)>>>
|
2221 TCGGCATGGACGAGCTGTACAAGTAActtaaggggtggcatccctgtgacccctccccag 2280
AGCCGTACCTGCTCGACATGTTCATTGAATTCCCCACCGTAGGGACACTGGGGAGGGGTC
2281 tgcctctcctggccctggaagttgccactccagtgcccaccagccttgtcctaataaaat 2340
ACGGAGAGGACCGGGACCTTCAACGGTGAGGTCACGGGTGGTCGGAACAGGATTATTTTA
2341 taagttgcatcattttgtctgactaggtgtccttctataatattatggggtggagggggg 2400
ATTCAACGTAGTAAAACAGACTGATCCACAGGAAGATATTATAATACCCCACCTCCCCCC
2401 tggtatggagcaaggggcaagttgggaagacaacctgtagggcctgcggggtctattggg 2460
ACCATACCTCGTTCCCCGTTCAACCCTTCTGTTGGACATCCCGGACGCCCCAGATAACCC
2461 aaccaagctggagtgcagtggcacaatcttggctcactgcaatctccgcctcctgggttc 2520
TTGGTTCGACCTCACGTCACCGTGTTAGAACCGAGTGACGTTAGAGGCGGAGGACCCAAG
SphI
|
2521 aagcgattctcctgcctcagcctcccgagttgttgggattccaggcatgcatgaccaggc 2580
TTCGCTAAGAGGACGGAGTCGGAGGGCTCAACAACCCTAAGGTCCGTACGTACTGGTCCG
2581 tcagctaatttttgtttttttggtagagacggggtttcaccatattggccaggctggtct 2640
AGTCGATTAAAAACAAAAAAACCATCTCTGCCCCAAAGTGGTATAACCGGTCCGACCAGA
2641 ccaactcctaatctcaggtgatctacccaccttggcctcccaaattgctgggattacagg 2700
GGTTGAGGATTAGAGTCCACTAGATGGGTGGAACCGGAGGGTTTAACGACCCTAATGTCC
2701 cgtgaaccactgctcccttccctgtccttcacgtgcggaccgagcggccgcaggaacccc 2760
GCACTTGGTGACGAGGGAAGGGACAGGAAGTGCACGCCTGGCTCGCCGGCGTCCTTGGGG
2761 tagtgatggagttggccactccctctctgcgcgctcgctcgctcactgaggccgggcgac 2820
ATCACTACCTCAACCGGTGAGGGAGAGACGCGCGAGCGAGCGAGTGACTCCGGCCCGCTG
2821 caaaggtcgcccgacgcccgggctttgcccgggcggcctcagtgagcgagcgagcgcgca 2880
GTTTCCAGCGGGCTGCGGGCCCGAAACGGGCCCGCCGGAGTCACTCGCTCGCTCGCGCGT
NarI
|
2881 gctgcctgcaggggcgcctgatgcggtattttctccttacgcatctgtgcggtatttcac 2940
CGACGGACGTCCCCGCGGACTACGCCATAAAAGAGGAATGCGTAGACACGCCATAAAGTG
f1 origin(2984,3290)>>>
|
2941 accgcatacgtcaaagcaaccatagtacgcgccctgtagcggcgcattaagcgcggcggg 3000
TGGCGTATGCAGTTTCGTTGGTATCATGCGCGGGACATCGCCGCGTAATTCGCGCCGCCC
3001 tgtggtggttacgcgcagcgtgaccgctacacttgccagcgccttagcgcccgctccttt 3060
ACACCACCAATGCGCGTCGCACTGGCGATGTGAACGGTCGCGGAATCGCGGGCGAGGAAA
3061 cgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcg 3120
GCGAAAGAAGGGAAGGAAAGAGCGGTGCAAGCGGCCGAAAGGGGCAGTTCGAGATTTAGC
3121 ggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttga 3180
CCCCGAGGGAAATCCCAAGGCTAAATCACGAAATGCCGTGGAGCTGGGGTTTTTTGAACT
3181 tttgggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgac 3240
AAACCCACTACCAAGTGCATCACCCGGTAGCGGGACTATCTGCCAAAAAGCGGGAAACTG
3241 gttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaactc 3300
CAACCTCAGGTGCAAGAAATTATCACCTGAGAACAAGGTTTGACCTTGTTGTGAGTTGAG
3301 tatctcgggctattcttttgatttataagggattttgccgatttcggtctattggttaaa 3360
ATAGAGCCCGATAAGAAAACTAAATATTCCCTAAAACGGCTAAAGCCAGATAACCAATTT
3361 aaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaat 3420
TTTACTCGACTAAATTGTTTTTAAATTGCGCTTAAAATTGTTTTATAATTGCAAATGTTA
3421 tttatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccccgaca 3480
AAATACCACGTGAGAGTCATGTTAGACGAGACTACGGCGTATCAATTCGGTCGGGGCTGT
3481 cccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacag 3540
GGGCGGTTGTGGGCGACTGCGCGGGACTGCCCGAACAGACGAGGGCCGTAGGCGAATGTC
3541 acaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaa 3600
TGTTCGACACTGGCAGAGGCCCTCGACGTACACAGTCTCCAAAAGTGGCAGTAGTGGCTT
3601 acgcgcgagccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcga 3660
TGCGCGCTCGGCCCTCGACGTACACAGTCTCCAAAAGTGGCAGTAGTGGCTTTGCGCGCT
3661 gacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggttt 3720
CTGCTTTCCCGGAGCACTATGCGGATAAAAATATCCAATTACAGTACTATTATTACCAAA
3721 cttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttt 3780
GAATCTGCAGTCCACCGTGAAAAGCCCCTTTACACGCGCCTTGGGGATAAACAAATAAAA
amp prom(3791,3819)>>>
|
3781 tctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaat 3840
AGATTTATGTAAGTTTATACATAGGCGAGTACTCTGTTATTGGGACTATTTACGAAGTTA
amp marker(3861,4721)>>>
|
ORF_2 rf(3)(3861,4721)>>>
|
3841 aatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctttt 3900
TTATAACTTTTTCCTTCTCATACTCATAAGTTGTAAAGGCACAGCGGGAATAAGGGAAAA
3901 ttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatg 3960
AACGCCGTAAAACGGAAGGACAAAAACGAGTGGGTCTTTGCGACCACTTTCATTTTCTAC
3961 ctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaaga 4020
GACTTCTAGTCAACCCACGTGCTCACCCAATGTAGCTTGACCTAGAGTTGTCGCCATTCT
4021 tccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgc 4080
AGGAACTCTCAAAAGCGGGGCTTCTTGCAAAAGGTTACTACTCGTGAAAATTTCAAGACG
4081 tatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatac 4140
ATACACCGCGCCATAATAGGGCATAACTGCGGCCCGTTCTCGTTGAGCCAGCGGCGTATG
4141 actattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatg 4200
TGATAAGAGTCTTACTGAACCAACTCATGAGTGGTCAGTGTCTTTTCGTAGAATGCCTAC
4201 gcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggcca 4260
CGTACTGTCATTCTCTTAATACGTCACGACGGTATTGGTACTCACTATTGTGACGCCGGT
4261 acttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgg 4320
TGAATGAAGACTGTTGCTAGCCTCCTGGCTTCCTCGATTGGCGAAAAAACGTGTTGTACC
4321 gggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacg 4380
CCCTAGTACATTGAGCGGAACTAGCAACCCTTGGCCTCGACTTACTTCGGTATGGTTTGC
4381 acgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactg 4440
TGCTCGCACTGTGGTGCTACGGACATCGTTACCGTTGTTGCAACGCGTTTGATAATTGAC
4441 gcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaag 4500
CGCTTGATGAATGAGATCGAAGGGCCGTTGTTAATTATCTGACCTACCTCCGCCTATTTC
4501 ttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctg 4560
AACGTCCTGGTGAAGACGCGAGCCGGGAAGGCCGACCGACCAAATAACGACTATTTAGAC
4561 gagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccct 4620
CTCGGCCACTCGCACCCAGAGCGCCATAGTAACGTCGTGACCCCGGTCTACCATTCGGGA
4621 cccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagac 4680
GGGCATAGCATCAATAGATGTGCTGCCCCTCAGTCCGTTGATACCTACTTGCTTTATCTG
4681 agatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttact 4740
TCTAGCGACTCTATCCACGGAGTGACTAATTCGTAACCATTGACAGTCTGGTTCAAATGA
4741 catatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaaga 4800
GTATATATGAAATCTAACTAAATTTTGAAGTAAAAATTAAATTTTCCTAGATCCACTTCT
4801 tcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgt 4860
AGGAAAAACTATTAGAGTACTGGTTTTAGGGAATTGCACTCAAAAGCAAGGTGACTCGCA
pBR322 origin(4876,5495)>>>
|
4861 cagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatct 4920
GTCTGGGGCATCTTTTCTAGTTTCCTAGAAGAACTCTAGGAAAAAAAGACGCGCATTAGA
4921 gctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagc 4980
CGACGAACGTTTGTTTTTTTGGTGGCGATGGTCGCCACCAAACAAACGGCCTAGTTCTCG
4981 taccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgttc 5040
ATGGTTGAGAAAAAGGCTTCCATTGACCGAAGTCGTCTCGCGTCTATGGTTTATGACAAG
5041 ttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacc 5100
AAGATCACATCGGCATCAATCCGGTGGTGAAGTTCTTGAGACATCGTGGCGGATGTATGG
AlwNI
|
5101 tcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccg 5160
AGCGAGACGATTAGGACAATGGTCACCGACGACGGTCACCGCTATTCAGCACAGAATGGC
5161 ggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggtt 5220
CCAACCTGAGTTCTGCTATCAATGGCCTATTCCGCGTCGCCAGCCCGACTTGCCCCCCAA
5221 cgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtg 5280
GCACGTGTGTCGGGTCGAACCTCGCTTGCTGGATGTGGCTTGACTCTATGGATGTCGCAC
5281 agctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcg 5340
TCGATACTCTTTCGCGGTGCGAAGGGCTTCCCTCTTTCCGCCTGTCCATAGGCCATTCGC
5341 gcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatcttt 5400
CGTCCCAGCCTTGTCCTCTCGCGTGCTCCCTCGAAGGTCCCCCTTTGCGGACCATAGAAA
5401 atagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcag 5460
TATCAGGACAGCCCAAAGCGGTGGAGACTGAACTCGCAGCTAAAAACACTACGAGCAGTC
5461 gggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggcctttt 5520
CCCCCGCCTCGGATACCTTTTTGCGGTCGTTGCGCCGGAAAAATGCCAAGGACCGGAAAA
5521 gctggccttttgctcacatgt 5541
CGACCGGAAAACGAGTGTACA
FEATURES Location/Qualifiers
Promoter 591..672
/gene="CMV prom"
misc_binding 647..652
/dbxref="REBASE:SacI"
Regulatory_Seq 728..767
/gene="tetO reg"
misc_binding 744..749
/dbxref="REBASE:BglII"
misc_binding 774..779
/dbxref="REBASE:EcoRV"
misc_binding 783..788
/dbxref="REBASE:XhoI"
Promoter 1099..1378
/gene="cBA prom"
ORF 1509..2261
/sequence="ORF_3 rf(5)"
Reporter 1527..2243
/gene="EGFP reporter"
ORF 1527..2246
/sequence="ORF_1 rf(3)"
Terminator 2253..2729
/gene="hGH_PA term"
misc_binding 2565..2570
/dbxref="REBASE:SphI"
misc_binding 2893..2898
/dbxref="REBASE:NarI"
Rep_Origin 2984..3290
/gene="f1 origin"
Promoter 3791..3819
/gene="amp prom"
Marker 3861..4721
/gene="amp marker"
ORF 3861..4721
/sequence="ORF_2 rf(3)"
Rep_Origin 4876..5495
/gene="pBR322 origin"
misc_binding 5122..5130
/dbxref="REBASE:AlwNI"
BASE COUNT 1250 a 1532 c 1468 g 1291 t 0 others