pAAV-G-CMV-DIO
| Vector Name | pAAV-G-CMV-DIO |
| VectorType | AAV Vector |
| Antibiotic Information | Kanamycin |
| Sequencing Primers | CMV sequencing primer 5`---CGC AAA TGG GCG GTA GGC GTG---3` |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta acctgcgcgc tcgctcgctc
481 actgaggccg cccgggcaaa gcccgggcgt cgggcgacct ttggtcgccc ggcctcagtg
541 agcgagcgag cgcgcagaga gggagtggcc aactccatca ctaggggttc cttgtagtta
601 atgattaacc cgccatgcta cttatctacg tagccatgct ctaggaagat cgcctaggcg
661 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
721 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
781 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
841 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
901 catgacctta tgggactttc ctacttggca gtacatctac gtttagtcat cgctattacc
961 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
1021 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
1081 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
1141 cggtgggagg tctatataag cagagctggt accaatcagc tcgctcacca tataacttcg
1201 tataaagtat cctatacgaa gttatatcaa aataggaaga ccaatgcttc accatcgacc
1261 cgaattgcca agcatcacca tcgacccata acttcgtata atgtatgcta tacgaagtta
1321 tctcgagtca gatatcaacc actttgtaca agaaagctga acgagaaacg taaaatgata
1381 taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa
1441 cacaacatat ccagtcacta tggtcgacct gcagactggc tgtgtataag ggagcctgac
1501 atttatattc cccagaacat caggttaatg gcgtttttga tgtcattttc gcggtggctg
1561 agatcagcca cttcttcccc gataacggag accggcacac tggccatatc ggtggtcatc
1621 atgcgccagc tttcatcccc gatatgcacc accgggtaaa gttcacggga gactttatct
1681 gacagcagac gtgcactggc cagggggatc accatccgtc gcccgggcgt gtcaataata
1741 tcactctgta catccacaaa cagacgataa cggctctctc ttttataggt gtaaacctta
1801 aactgcattt caccagcccc tgttctcgtc agcaaaagag ccgttcattt caataaaccg
1861 ggcgacctca gccatccctt cctgattttc cgctttccag cgttcggcac gcagacgacg
1921 ggcttcattc tgcatggttg tgcttaccag accggagata ttgacatcat atatgccttg
1981 agcaactgat agctgtcgct gtcaactgtc actgtaatac gctgcttcat agcatacctc
2041 tttttgacat acttcgggta tacatatcag tatatattct tataccgcaa aaatcagcgc
2101 gcaaatacgc atactgttat ctggctttta gtaagccgga tccagatctt tacgccccgc
2161 cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat
2221 cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat
2281 aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat
2341 caaaactggt gaaactcacc cagggattgg ctgagacgaa aaacatattc tcaataaacc
2401 ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta
2461 gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct
2521 catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca
2581 ttgccatacg gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg
2641 gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa
2701 cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat
2761 gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct
2821 tagctcctga aaatctcgac ggatcctaac tcaaaatcca cacattatac gagccggaag
2881 cataaagtgt aaagcctggg gtgcctaatg cggccgccat agtgactgga tatgttgtgt
2941 tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat
3001 atcattttac gtttctcgtt cagctttttt gtacaaactt gttgatatcg ctagccaatt
3061 gataacttcg tataggatac tttatacgaa gttatcattg ggattcttcc tattttgatc
3121 caagcatcac catcgaccct ctagtccaga tctcaccatc gacccataac ttcgtatagc
3181 atacattata cgaagttata tggtgagcaa gggcgattct agaaacttgt ttattgcagc
3241 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
3301 actgcattct agttgtggtt tgtccaaact catcaatgta tcttaaagct taggaacccc
3361 tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac
3421 caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca
3481 gggatcccgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc
3541 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
3601 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
3661 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
3721 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
3781 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
3841 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
3901 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
3961 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
4021 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
4081 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
4141 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
4201 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
4261 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
4321 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
4381 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
4441 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
4501 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
4561 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
4621 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
4681 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
4741 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgaaa aaggatcttc
4801 acctagatcc ttttcacgta gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat
4861 gtcagctact gggctatctg gacaagggaa aacgcaagcg caaagagaaa gcaggtagct
4921 tgcagtgggc ttacatggcg atagctagac tgggcggttt tatggacagc aagcgaaccg
4981 gaattgccag ctggggcgcc ctctggtaag gttgggaagc cctgcaaagt aaactggatg
5041 gctttctcgc cgccaaggat ctgatggcgc aggggatcaa gctctgatca agagacagga
5101 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg
5161 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
5221 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
5281 gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt
5341 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
5401 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc
5461 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
5521 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
5581 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag
5641 gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat
5701 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
5761 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
5821 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
5881 ttctatcgcc ttcttgacga gttcttctga attttgttaa aatttttgtt aaatcagctc
5941 attttttaac caataggccg aaatcggcaa catcccttat aaatcaaaag aatagaccgc
6001 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
6061 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
6121 caaatcaagt tttttgcggt cgaggtgccg taaagctcta aatcggaacc ctaaagggag
6181 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
6241 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
6301 cacacccgcg cgcttaatgc gccgctacag ggcgcgtcca ttcgccattc aggatcgaat
6361 taattcttaa ttaacatcat caataatata cctt
LOCUS dna 6394 bp
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
misc_binding 457..462
/dbxref="REBASE:HpaI"
misc_binding 833..838
/dbxref="REBASE:NdeI"
misc_binding 1168..1173
/dbxref="REBASE:KpnI"
Other Gene 1288..1321
/gene="loxP other"
misc_binding 1322..1327
/dbxref="REBASE:XhoI"
Other Gene 1343..1462
/gene="attR1 other"
Other Gene 1362..1462
/gene="attR2 other"
misc_binding 1469..1474
/dbxref="REBASE:PstI"
Other Gene 1503..1808
/gene="ccdB other"
Marker 2150..2809
/gene="CAT marker"
ORF 2150..2809
/sequence="ORF_2 rf(4)"
misc_binding 2591..2596
/dbxref="REBASE:EcoRI"
misc_binding 2910..2917
/dbxref="REBASE:NotI"
Other Gene 2918..3042
/gene="attR1 other"
Other Gene 2918..3018
/gene="attR2 other"
Other Gene 3166..3199
/gene="loxP other"
misc_binding 3218..3223
/dbxref="REBASE:XbaI"
misc_binding 3346..3351
/dbxref="REBASE:HindIII"
Rep_Origin 3706..4325
/gene="pBR322 origin"
Promoter 4978..5027
/gene="NEOKAN prom"
misc_binding 5085..5090
/dbxref="REBASE:BclI"
ORF 5116..5910
/sequence="ORF_1 rf(1)"
Marker 5119..5907
/gene="NTP_II marker"
misc_binding 5645..5650
/dbxref="REBASE:SphI"
Rep_Origin 6015..6320
/gene="f1 origin"
BASE COUNT 1641 a 1569 c 1587 g 1597 t 0 others
ORIGIN
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta acctgcgcgc tcgctcgctc
481 actgaggccg cccgggcaaa gcccgggcgt cgggcgacct ttggtcgccc ggcctcagtg
541 agcgagcgag cgcgcagaga gggagtggcc aactccatca ctaggggttc cttgtagtta
601 atgattaacc cgccatgcta cttatctacg tagccatgct ctaggaagat cgcctaggcg
661 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
721 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
781 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
841 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
901 catgacctta tgggactttc ctacttggca gtacatctac gtttagtcat cgctattacc
961 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
1021 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
1081 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
1141 cggtgggagg tctatataag cagagctggt accaatcagc tcgctcacca tataacttcg
1201 tataaagtat cctatacgaa gttatatcaa aataggaaga ccaatgcttc accatcgacc
1261 cgaattgcca agcatcacca tcgacccata acttcgtata atgtatgcta tacgaagtta
1321 tctcgagtca gatatcaacc actttgtaca agaaagctga acgagaaacg taaaatgata
1381 taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa
1441 cacaacatat ccagtcacta tggtcgacct gcagactggc tgtgtataag ggagcctgac
1501 atttatattc cccagaacat caggttaatg gcgtttttga tgtcattttc gcggtggctg
1561 agatcagcca cttcttcccc gataacggag accggcacac tggccatatc ggtggtcatc
1621 atgcgccagc tttcatcccc gatatgcacc accgggtaaa gttcacggga gactttatct
1681 gacagcagac gtgcactggc cagggggatc accatccgtc gcccgggcgt gtcaataata
1741 tcactctgta catccacaaa cagacgataa cggctctctc ttttataggt gtaaacctta
1801 aactgcattt caccagcccc tgttctcgtc agcaaaagag ccgttcattt caataaaccg
1861 ggcgacctca gccatccctt cctgattttc cgctttccag cgttcggcac gcagacgacg
1921 ggcttcattc tgcatggttg tgcttaccag accggagata ttgacatcat atatgccttg
1981 agcaactgat agctgtcgct gtcaactgtc actgtaatac gctgcttcat agcatacctc
2041 tttttgacat acttcgggta tacatatcag tatatattct tataccgcaa aaatcagcgc
2101 gcaaatacgc atactgttat ctggctttta gtaagccgga tccagatctt tacgccccgc
2161 cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat
2221 cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat
2281 aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat
2341 caaaactggt gaaactcacc cagggattgg ctgagacgaa aaacatattc tcaataaacc
2401 ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta
2461 gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct
2521 catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca
2581 ttgccatacg gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg
2641 gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa
2701 cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat
2761 gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct
2821 tagctcctga aaatctcgac ggatcctaac tcaaaatcca cacattatac gagccggaag
2881 cataaagtgt aaagcctggg gtgcctaatg cggccgccat agtgactgga tatgttgtgt
2941 tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat
3001 atcattttac gtttctcgtt cagctttttt gtacaaactt gttgatatcg ctagccaatt
3061 gataacttcg tataggatac tttatacgaa gttatcattg ggattcttcc tattttgatc
3121 caagcatcac catcgaccct ctagtccaga tctcaccatc gacccataac ttcgtatagc
3181 atacattata cgaagttata tggtgagcaa gggcgattct agaaacttgt ttattgcagc
3241 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
3301 actgcattct agttgtggtt tgtccaaact catcaatgta tcttaaagct taggaacccc
3361 tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac
3421 caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca
3481 gggatcccgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc
3541 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
3601 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
3661 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
3721 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
3781 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
3841 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
3901 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
3961 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
4021 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
4081 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
4141 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
4201 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
4261 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
4321 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
4381 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
4441 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
4501 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
4561 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
4621 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
4681 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
4741 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgaaa aaggatcttc
4801 acctagatcc ttttcacgta gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat
4861 gtcagctact gggctatctg gacaagggaa aacgcaagcg caaagagaaa gcaggtagct
4921 tgcagtgggc ttacatggcg atagctagac tgggcggttt tatggacagc aagcgaaccg
4981 gaattgccag ctggggcgcc ctctggtaag gttgggaagc cctgcaaagt aaactggatg
5041 gctttctcgc cgccaaggat ctgatggcgc aggggatcaa gctctgatca agagacagga
5101 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg
5161 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
5221 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
5281 gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt
5341 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
5401 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc
5461 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
5521 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
5581 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag
5641 gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat
5701 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
5761 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
5821 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
5881 ttctatcgcc ttcttgacga gttcttctga attttgttaa aatttttgtt aaatcagctc
5941 attttttaac caataggccg aaatcggcaa catcccttat aaatcaaaag aatagaccgc
6001 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
6061 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
6121 caaatcaagt tttttgcggt cgaggtgccg taaagctcta aatcggaacc ctaaagggag
6181 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
6241 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
6301 cacacccgcg cgcttaatgc gccgctacag ggcgcgtcca ttcgccattc aggatcgaat
6361 taattcttaa ttaacatcat caataatata cctt
//
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 ttttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgt 60
AAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCA
61 gggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaa 120
CCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTT
Encap other(162,310)>>>
|
121 cacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgccggtgtacacagg 180
GTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCC
181 aagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagt 240
TTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCA
241 aagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttg 300
TTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAAC
301 tgttactcatagcgcgtaatacggcagacctcagcgctagattattgaagcatttatcag 360
ACAATGAGTATCGCGCATTATGCCGTCTGGAGTCGCGATCTAATAACTTCGTAAATAGTC
amp prom(365,393)<<<
|
361 ggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggg 420
CCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCC
HpaI
|
421 gttccgcgcacatttccccgaaaagtgccacctgacgttaacctgcgcgctcgctcgctc 480
CAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAATTGGACGCGCGAGCGAGCGAG
481 actgaggccgcccgggcaaagcccgggcgtcgggcgacctttggtcgcccggcctcagtg 540
TGACTCCGGCGGGCCCGTTTCGGGCCCGCAGCCCGCTGGAAACCAGCGGGCCGGAGTCAC
541 agcgagcgagcgcgcagagagggagtggccaactccatcactaggggttccttgtagtta 600
TCGCTCGCTCGCGCGTCTCTCCCTCACCGGTTGAGGTAGTGATCCCCAAGGAACATCAAT
601 atgattaacccgccatgctacttatctacgtagccatgctctaggaagatcgcctaggcg 660
TACTAATTGGGCGGTACGATGAATAGATGCATCGGTACGAGATCCTTCTAGCGGATCCGC
661 cgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccatt 720
GCAATGTATTGAATGCCATTTACCGGGCGGACCGACTGGCGGGTTGCTGGGGGCGGGTAA
721 gacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtca 780
CTGCAGTTATTACTGCATACAAGGGTATCATTGCGGTTATCCCTGAAAGGTAACTGCAGT
NdeI
|
781 atgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgcc 840
TACCCACCTCATAAATGCCATTTGACGGGTGAACCGTCATGTAGTTCACATAGTATACGG
841 aagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagta 900
TTCATGCGGGGGATAACTGCAGTTACTGCCATTTACCGGGCGGACCGTAATACGGGTCAT
901 catgaccttatgggactttcctacttggcagtacatctacgtttagtcatcgctattacc 960
GTACTGGAATACCCTGAAAGGATGAACCGTCATGTAGATGCAAATCAGTAGCGATAATGG
961 atggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacgggga 1020
TACCACTACGCCAAAACCGTCATGTAGTTACCCGCACCTATCGCCAAACTGAGTGCCCCT
1021 tttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgg 1080
AAAGGTTCAGAGGTGGGGTAACTGCAGTTACCCTCAAACAAAACCGTGGTTTTAGTTGCC
1081 gactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgta 1140
CTGAAAGGTTTTACAGCATTGTTGAGGCGGGGTAACTGCGTTTACCCGCCATCCGCACAT
KpnI
|
1141 cggtgggaggtctatataagcagagctggtaccaatcagctcgctcaccatataacttcg 1200
GCCACCCTCCAGATATATTCGTCTCGACCATGGTTAGTCGAGCGAGTGGTATATTGAAGC
1201 tataaagtatcctatacgaagttatatcaaaataggaagaccaatgcttcaccatcgacc 1260
ATATTTCATAGGATATGCTTCAATATAGTTTTATCCTTCTGGTTACGAAGTGGTAGCTGG
loxP other(1288,1321)<<<
|
1261 cgaattgccaagcatcaccatcgacccataacttcgtataatgtatgctatacgaagtta 1320
GCTTAACGGTTCGTAGTGGTAGCTGGGTATTGAAGCATATTACATACGATATGCTTCAAT
attR2 other(1362,1462)<<<
|
XhoI attR1 other(1343,1462)>>>
| | |
1321 tctcgagtcagatatcaaccactttgtacaagaaagctgaacgagaaacgtaaaatgata 1380
AGAGCTCAGTCTATAGTTGGTGAAACATGTTCTTTCGACTTGCTCTTTGCATTTTACTAT
1381 taaatatcaatatattaaattagattttgcataaaaaacagactacataatactgtaaaa 1440
ATTTATAGTTATATAATTTAATCTAAAACGTATTTTTTGTCTGATGTATTATGACATTTT
PstI
|
1441 cacaacatatccagtcactatggtcgacctgcagactggctgtgtataagggagcctgac 1500
GTGTTGTATAGGTCAGTGATACCAGCTGGACGTCTGACCGACACATATTCCCTCGGACTG
ccdB other(1503,1808)<<<
|
1501 atttatattccccagaacatcaggttaatggcgtttttgatgtcattttcgcggtggctg 1560
TAAATATAAGGGGTCTTGTAGTCCAATTACCGCAAAAACTACAGTAAAAGCGCCACCGAC
1561 agatcagccacttcttccccgataacggagaccggcacactggccatatcggtggtcatc 1620
TCTAGTCGGTGAAGAAGGGGCTATTGCCTCTGGCCGTGTGACCGGTATAGCCACCAGTAG
1621 atgcgccagctttcatccccgatatgcaccaccgggtaaagttcacgggagactttatct 1680
TACGCGGTCGAAAGTAGGGGCTATACGTGGTGGCCCATTTCAAGTGCCCTCTGAAATAGA
1681 gacagcagacgtgcactggccagggggatcaccatccgtcgcccgggcgtgtcaataata 1740
CTGTCGTCTGCACGTGACCGGTCCCCCTAGTGGTAGGCAGCGGGCCCGCACAGTTATTAT
1741 tcactctgtacatccacaaacagacgataacggctctctcttttataggtgtaaacctta 1800
AGTGAGACATGTAGGTGTTTGTCTGCTATTGCCGAGAGAGAAAATATCCACATTTGGAAT
1801 aactgcatttcaccagcccctgttctcgtcagcaaaagagccgttcatttcaataaaccg 1860
TTGACGTAAAGTGGTCGGGGACAAGAGCAGTCGTTTTCTCGGCAAGTAAAGTTATTTGGC
1861 ggcgacctcagccatcccttcctgattttccgctttccagcgttcggcacgcagacgacg 1920
CCGCTGGAGTCGGTAGGGAAGGACTAAAAGGCGAAAGGTCGCAAGCCGTGCGTCTGCTGC
1921 ggcttcattctgcatggttgtgcttaccagaccggagatattgacatcatatatgccttg 1980
CCGAAGTAAGACGTACCAACACGAATGGTCTGGCCTCTATAACTGTAGTATATACGGAAC
1981 agcaactgatagctgtcgctgtcaactgtcactgtaatacgctgcttcatagcatacctc 2040
TCGTTGACTATCGACAGCGACAGTTGACAGTGACATTATGCGACGAAGTATCGTATGGAG
2041 tttttgacatacttcgggtatacatatcagtatatattcttataccgcaaaaatcagcgc 2100
AAAAACTGTATGAAGCCCATATGTATAGTCATATATAAGAATATGGCGTTTTTAGTCGCG
ORF_2 rf(4)(2150,2809)<<<
|
CAT marker(2150,2809)<<<
|
2101 gcaaatacgcatactgttatctggcttttagtaagccggatccagatctttacgccccgc 2160
CGTTTATGCGTATGACAATAGACCGAAAATCATTCGGCCTAGGTCTAGAAATGCGGGGCG
2161 cctgccactcatcgcagtactgttgtaattcattaagcattctgccgacatggaagccat 2220
GGACGGTGAGTAGCGTCATGACAACATTAAGTAATTCGTAAGACGGCTGTACCTTCGGTA
2221 cacaaacggcatgatgaacctgaatcgccagcggcatcagcaccttgtcgccttgcgtat 2280
GTGTTTGCCGTACTACTTGGACTTAGCGGTCGCCGTAGTCGTGGAACAGCGGAACGCATA
2281 aatatttgcccatggtgaaaacgggggcgaagaagttgtccatattggccacgtttaaat 2340
TTATAAACGGGTACCACTTTTGCCCCCGCTTCTTCAACAGGTATAACCGGTGCAAATTTA
2341 caaaactggtgaaactcacccagggattggctgagacgaaaaacatattctcaataaacc 2400
GTTTTGACCACTTTGAGTGGGTCCCTAACCGACTCTGCTTTTTGTATAAGAGTTATTTGG
2401 ctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtgta 2460
GAAATCCCTTTATCCGGTCCAAAAGTGGCATTGTGCGGTGTAGAACGCTTATATACACAT
2461 gaaactgccggaaatcgtcgtggtattcactccagagcgatgaaaacgtttcagtttgct 2520
CTTTGACGGCCTTTAGCAGCACCATAAGTGAGGTCTCGCTACTTTTGCAAAGTCAAACGA
2521 catggaaaacggtgtaacaagggtgaacactatcccatatcaccagctcaccgtctttca 2580
GTACCTTTTGCCACATTGTTCCCACTTGTGATAGGGTATAGTGGTCGAGTGGCAGAAAGT
EcoRI
|
2581 ttgccatacggaattccggatgagcattcatcaggcgggcaagaatgtgaataaaggccg 2640
AACGGTATGCCTTAAGGCCTACTCGTAAGTAGTCCGCCCGTTCTTACACTTATTTCCGGC
2641 gataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaatatccagctgaa 2700
CTATTTTGAACACGAATAAAAAGAAATGCCAGAAATTTTTCCGGCATTATAGGTCGACTT
2701 cggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctttacgat 2760
GCCAGACCAATATCCATGTAACTCGTTGACTGACTTTACGGAGTTTTACAAGAAATGCTA
2761 gccattgggatatatcaacggtggtatatccagtgatttttttctccattttagcttcct 2820
CGGTAACCCTATATAGTTGCCACCATATAGGTCACTAAAAAAAGAGGTAAAATCGAAGGA
2821 tagctcctgaaaatctcgacggatcctaactcaaaatccacacattatacgagccggaag 2880
ATCGAGGACTTTTAGAGCTGCCTAGGATTGAGTTTTAGGTGTGTAATATGCTCGGCCTTC
attR1 other(2918,3042)<<<
|
NotI attR2 other(2918,3018)>>>
| |
2881 cataaagtgtaaagcctggggtgcctaatgcggccgccatagtgactggatatgttgtgt 2940
GTATTTCACATTTCGGACCCCACGGATTACGCCGGCGGTATCACTGACCTATACAACACA
2941 tttacagtattatgtagtctgttttttatgcaaaatctaatttaatatattgatatttat 3000
AAATGTCATAATACATCAGACAAAAAATACGTTTTAGATTAAATTATATAACTATAAATA
3001 atcattttacgtttctcgttcagcttttttgtacaaacttgttgatatcgctagccaatt 3060
TAGTAAAATGCAAAGAGCAAGTCGAAAAAACATGTTTGAACAACTATAGCGATCGGTTAA
3061 gataacttcgtataggatactttatacgaagttatcattgggattcttcctattttgatc 3120
CTATTGAAGCATATCCTATGAAATATGCTTCAATAGTAACCCTAAGAAGGATAAAACTAG
loxP other(3166,3199)>>>
|
3121 caagcatcaccatcgaccctctagtccagatctcaccatcgacccataacttcgtatagc 3180
GTTCGTAGTGGTAGCTGGGAGATCAGGTCTAGAGTGGTAGCTGGGTATTGAAGCATATCG
XbaI
|
3181 atacattatacgaagttatatggtgagcaagggcgattctagaaacttgtttattgcagc 3240
TATGTAATATGCTTCAATATACCACTCGTTCCCGCTAAGATCTTTGAACAAATAACGTCG
3241 ttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttc 3300
AATATTACCAATGTTTATTTCGTTATCGTAGTGTTTAAAGTGTTTATTTCGTAAAAAAAG
HindIII
|
3301 actgcattctagttgtggtttgtccaaactcatcaatgtatcttaaagcttaggaacccc 3360
TGACGTAAGATCAACACCAAACAGGTTTGAGTAGTTACATAGAATTTCGAATCCTTGGGG
3361 tagtgatggagttggccactccctctctgcgcgctcgctcgctcactgaggccgggcgac 3420
ATCACTACCTCAACCGGTGAGGGAGAGACGCGCGAGCGAGCGAGTGACTCCGGCCCGCTG
3421 caaaggtcgcccgacgcccgggctttgcccgggcggcctcagtgagcgagcgagcgcgca 3480
GTTTCCAGCGGGCTGCGGGCCCGAAACGGGCCCGCCGGAGTCACTCGCTCGCTCGCGCGT
3481 gggatcccggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgctc 3540
CCCTAGGGCCACACTTTATGGCGTGTCTACGCATTCCTCTTTTATGGCGTAGTCCGCGAG
3541 ttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatc 3600
AAGGCGAAGGAGCGAGTGACTGAGCGACGCGAGCCAGCAAGCCGACGCCGCTCGCCATAG
3601 agctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaa 3660
TCGAGTGAGTTTCCGCCATTATGCCAATAGGTGTCTTAGTCCCCTATTGCGTCCTTTCTT
pBR322 origin(3706,4325)<<<
|
3661 catgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtt 3720
GTACACTCGTTTTCCGGTCGTTTTCCGGTCCTTGGCATTTTTCCGGCGCAACGACCGCAA
3721 tttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtg 3780
AAAGGTATCCGAGGCGGGGGGACTGCTCGTAGTGTTTTTAGCTGCGAGTTCAGTCTCCAC
3781 gcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcg 3840
CGCTTTGGGCTGTCCTGATATTTCTATGGTCCGCAAAGGGGGACCTTCGAGGGAGCACGC
3841 ctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaag 3900
GAGAGGACAAGGCTGGGACGGCGAATGGCCTATGGACAGGCGGAAAGAGGGAAGCCCTTC
3901 cgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctc 3960
GCACCGCGAAAGAGTATCGAGTGCGACATCCATAGAGTCAAGCCACATCCAGCAAGCGAG
3961 caagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaa 4020
GTTCGACCCGACACACGTGCTTGGGGGGCAAGTCGGGCTGGCGACGCGGAATAGGCCATT
4021 ctatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactgg 4080
GATAGCAGAACTCAGGTTGGGCCATTCTGTGCTGAATAGCGGTGACCGTCGTCGGTGACC
4081 taacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcc 4140
ATTGTCCTAATCGTCTCGCTCCATACATCCGCCACGATGTCTCAAGAACTTCACCACCGG
4141 taactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttac 4200
ATTGATGCCGATGTGATCTTCCTGTCATAAACCATAGACGCGAGACGACTTCGGTCAATG
4201 cttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtgg 4260
GAAGCCTTTTTCTCAACCATCGAGAACTAGGCCGTTTGTTTGGTGGCGACCATCGCCACC
4261 tttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatccttt 4320
AAAAAAACAAACGTTCGTCGTCTAATGCGCGTCTTTTTTTCCTAGAGTTCTTCTAGGAAA
4321 gatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggt 4380
CTAGAAAAGATGCCCCAGACTGCGAGTCACCTTGCTTTTGAGTGCAATTCCCTAAAACCA
4381 catgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaa 4440
GTACTCTAATAGTTTTTCCTAGAAGTGGATCTAGGAAAATTTAATTTTTACTTCAAAATT
4441 atcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtga 4500
TAGTTAGATTTCATATATACTCATTTGAACCAGACTGTCAATGGTTACGAATTAGTCACT
4501 ggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgt 4560
CCGTGGATAGAGTCGCTAGACAGATAAAGCAAGTAGGTATCAACGGACTGAGGGGCAGCA
4561 gtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcg 4620
CATCTATTGATGCTATGCCCTCCCGAATGGTAGACCGGGGTCACGACGTTACTATGGCGC
4621 agacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccga 4680
TCTGGGTGCGAGTGGCCGAGGTCTAAATAGTCGTTATTTGGTCGGTCGGCCTTCCCGGCT
4681 gcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccggga 4740
CGCGTCTTCACCAGGACGTTGAAATAGGCGGAGGTAGGTCAGATAATTAACAACGGCCCT
4741 agctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgaaaaaggatcttc 4800
TCGATCTCATTCATCAAGCGGTCAATTATCAAACGCGTTGCAACAACTTTTTCCTAGAAG
4801 acctagatccttttcacgtagaaagccagtccgcagaaacggtgctgaccccggatgaat 4860
TGGATCTAGGAAAAGTGCATCTTTCGGTCAGGCGTCTTTGCCACGACTGGGGCCTACTTA
4861 gtcagctactgggctatctggacaagggaaaacgcaagcgcaaagagaaagcaggtagct 4920
CAGTCGATGACCCGATAGACCTGTTCCCTTTTGCGTTCGCGTTTCTCTTTCGTCCATCGA
NEOKAN prom(4978,5027)>>>
|
4921 tgcagtgggcttacatggcgatagctagactgggcggttttatggacagcaagcgaaccg 4980
ACGTCACCCGAATGTACCGCTATCGATCTGACCCGCCAAAATACCTGTCGTTCGCTTGGC
4981 gaattgccagctggggcgccctctggtaaggttgggaagccctgcaaagtaaactggatg 5040
CTTAACGGTCGACCCCGCGGGAGACCATTCCAACCCTTCGGGACGTTTCATTTGACCTAC
BclI
|
5041 gctttctcgccgccaaggatctgatggcgcaggggatcaagctctgatcaagagacagga 5100
CGAAAGAGCGGCGGTTCCTAGACTACCGCGTCCCCTAGTTCGAGACTAGTTCTCTGTCCT
NTP_II marker(5119,5907)>>>
|
ORF_1 rf(1)(5116,5910)>>>
| |
5101 tgaggatcgtttcgcatgattgaacaagatggattgcacgcaggttctccggccgcttgg 5160
ACTCCTAGCAAAGCGTACTAACTTGTTCTACCTAACGTGCGTCCAAGAGGCCGGCGAACC
5161 gtggagaggctattcggctatgactgggcacaacagacaatcggctgctctgatgccgcc 5220
CACCTCTCCGATAAGCCGATACTGACCCGTGTTGTCTGTTAGCCGACGAGACTACGGCGG
5221 gtgttccggctgtcagcgcaggggcgcccggttctttttgtcaagaccgacctgtccggt 5280
CACAAGGCCGACAGTCGCGTCCCCGCGGGCCAAGAAAAACAGTTCTGGCTGGACAGGCCA
5281 gccctgaatgaactgcaagacgaggcagcgcggctatcgtggctggccacgacgggcgtt 5340
CGGGACTTACTTGACGTTCTGCTCCGTCGCGCCGATAGCACCGACCGGTGCTGCCCGCAA
5341 ccttgcgcagctgtgctcgacgttgtcactgaagcgggaagggactggctgctattgggc 5400
GGAACGCGTCGACACGAGCTGCAACAGTGACTTCGCCCTTCCCTGACCGACGATAACCCG
5401 gaagtgccggggcaggatctcctgtcatctcaccttgctcctgccgagaaagtatccatc 5460
CTTCACGGCCCCGTCCTAGAGGACAGTAGAGTGGAACGAGGACGGCTCTTTCATAGGTAG
5461 atggctgatgcaatgcggcggctgcatacgcttgatccggctacctgcccattcgaccac 5520
TACCGACTACGTTACGCCGCCGACGTATGCGAACTAGGCCGATGGACGGGTAAGCTGGTG
5521 caagcgaaacatcgcatcgagcgagcacgtactcggatggaagccggtcttgtcgatcag 5580
GTTCGCTTTGTAGCGTAGCTCGCTCGTGCATGAGCCTACCTTCGGCCAGAACAGCTAGTC
5581 gatgatctggacgaagagcatcaggggctcgcgccagccgaactgttcgccaggctcaag 5640
CTACTAGACCTGCTTCTCGTAGTCCCCGAGCGCGGTCGGCTTGACAAGCGGTCCGAGTTC
SphI
|
5641 gcgagcatgcccgacggcgaggatctcgtcgtgacccatggcgatgcctgcttgccgaat 5700
CGCTCGTACGGGCTGCCGCTCCTAGAGCAGCACTGGGTACCGCTACGGACGAACGGCTTA
5701 atcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtgtggcg 5760
TAGTACCACCTTTTACCGGCGAAAAGACCTAAGTAGCTGACACCGGCCGACCCACACCGC
5761 gaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcgaa 5820
CTGGCGATAGTCCTGTATCGCAACCGATGGGCACTATAACGACTTCTCGAACCGCCGCTT
5821 tgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgcc 5880
ACCCGACTGGCGAAGGAGCACGAAATGCCATAGCGGCGAGGGCTAAGCGTCGCGTAGCGG
5881 ttctatcgccttcttgacgagttcttctgaattttgttaaaatttttgttaaatcagctc 5940
AAGATAGCGGAAGAACTGCTCAAGAAGACTTAAAACAATTTTAAAAACAATTTAGTCGAG
5941 attttttaaccaataggccgaaatcggcaacatcccttataaatcaaaagaatagaccgc 6000
TAAAAAATTGGTTATCCGGCTTTAGCCGTTGTAGGGAATATTTAGTTTTCTTATCTGGCG
f1 origin(6015,6320)<<<
|
6001 gatagggttgagtgttgttccagtttggaacaagagtccactattaaagaacgtggactc 6060
CTATCCCAACTCACAACAAGGTCAAACCTTGTTCTCAGGTGATAATTTCTTGCACCTGAG
6061 caacgtcaaagggcgaaaaaccgtctatcagggcgatggcccactacgtgaaccatcacc 6120
GTTGCAGTTTCCCGCTTTTTGGCAGATAGTCCCGCTACCGGGTGATGCACTTGGTAGTGG
6121 caaatcaagttttttgcggtcgaggtgccgtaaagctctaaatcggaaccctaaagggag 6180
GTTTAGTTCAAAAAACGCCAGCTCCACGGCATTTCGAGATTTAGCCTTGGGATTTCCCTC
6181 cccccgatttagagcttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaa 6240
GGGGGCTAAATCTCGAACTGCCCCTTTCGGCCGCTTGCACCGCTCTTTCCTTCCCTTCTT
6241 agcgaaaggagcgggcgctagggcgctggcaagtgtagcggtcacgctgcgcgtaaccac 6300
TCGCTTTCCTCGCCCGCGATCCCGCGACCGTTCACATCGCCAGTGCGACGCGCATTGGTG
6301 cacacccgcgcgcttaatgcgccgctacagggcgcgtccattcgccattcaggatcgaat 6360
GTGTGGGCGCGCGAATTACGCGGCGATGTCCCGCGCAGGTAAGCGGTAAGTCCTAGCTTA
6361 taattcttaattaacatcatcaataatatacctt 6394
ATTAAGAATTAATTGTAGTAGTTATTATATGGAA
LOCUS dna 6394 bp
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
misc_binding 457..462
/dbxref="REBASE:HpaI"
misc_binding 833..838
/dbxref="REBASE:NdeI"
misc_binding 1168..1173
/dbxref="REBASE:KpnI"
Other Gene 1288..1321
/gene="loxP other"
misc_binding 1322..1327
/dbxref="REBASE:XhoI"
Other Gene 1343..1462
/gene="attR1 other"
Other Gene 1362..1462
/gene="attR2 other"
misc_binding 1469..1474
/dbxref="REBASE:PstI"
Other Gene 1503..1808
/gene="ccdB other"
Marker 2150..2809
/gene="CAT marker"
ORF 2150..2809
/sequence="ORF_2 rf(4)"
misc_binding 2591..2596
/dbxref="REBASE:EcoRI"
misc_binding 2910..2917
/dbxref="REBASE:NotI"
Other Gene 2918..3042
/gene="attR1 other"
Other Gene 2918..3018
/gene="attR2 other"
Other Gene 3166..3199
/gene="loxP other"
misc_binding 3218..3223
/dbxref="REBASE:XbaI"
misc_binding 3346..3351
/dbxref="REBASE:HindIII"
Rep_Origin 3706..4325
/gene="pBR322 origin"
Promoter 4978..5027
/gene="NEOKAN prom"
misc_binding 5085..5090
/dbxref="REBASE:BclI"
ORF 5116..5910
/sequence="ORF_1 rf(1)"
Marker 5119..5907
/gene="NTP_II marker"
misc_binding 5645..5650
/dbxref="REBASE:SphI"
Rep_Origin 6015..6320
/gene="f1 origin"
BASE COUNT 1641 a 1569 c 1587 g 1597 t 0 others