pAAV-G-CMV-DIO-SV40-Puro
| Vector Name | pAAV-G-CMV-DIO-SV40-Puro |
| VectorType | AAV Vector |
| Antibiotic Information | Bacterial: Kanamycin Mammalian: Puromycin |
| Sequencing Primers | CMV sequencing primer 5`---CGC AAA TGG GCG GTA GGC GTG---3` |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta acctgcgcgc tcgctcgctc
481 actgaggccg cccgggcaaa gcccgggcgt cgggcgacct ttggtcgccc ggcctcagtg
541 agcgagcgag cgcgcagaga gggagtggcc aactccatca ctaggggttc cttgtagtta
601 atgattaacc cgccatgcta cttatctacg tagccatgct ctaggaagat cgcctaggcg
661 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
721 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
781 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
841 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
901 catgacctta tgggactttc ctacttggca gtacatctac gtttagtcat cgctattacc
961 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
1021 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
1081 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
1141 cggtgggagg tctatataag cagagctggt accaatcagc tcgctcacca tataacttcg
1201 tataaagtat cctatacgaa gttatatcaa aataggaaga ccaatgcttc accatcgacc
1261 cgaattgcca agcatcacca tcgacccata acttcgtata atgtatgcta tacgaagtta
1321 tctcgagtca gatatcaacc actttgtaca agaaagctga acgagaaacg taaaatgata
1381 taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa
1441 cacaacatat ccagtcacta tggtcgacct gcagactggc tgtgtataag ggagcctgac
1501 atttatattc cccagaacat caggttaatg gcgtttttga tgtcattttc gcggtggctg
1561 agatcagcca cttcttcccc gataacggag accggcacac tggccatatc ggtggtcatc
1621 atgcgccagc tttcatcccc gatatgcacc accgggtaaa gttcacggga gactttatct
1681 gacagcagac gtgcactggc cagggggatc accatccgtc gcccgggcgt gtcaataata
1741 tcactctgta catccacaaa cagacgataa cggctctctc ttttataggt gtaaacctta
1801 aactgcattt caccagcccc tgttctcgtc agcaaaagag ccgttcattt caataaaccg
1861 ggcgacctca gccatccctt cctgattttc cgctttccag cgttcggcac gcagacgacg
1921 ggcttcattc tgcatggttg tgcttaccag accggagata ttgacatcat atatgccttg
1981 agcaactgat agctgtcgct gtcaactgtc actgtaatac gctgcttcat agcatacctc
2041 tttttgacat acttcgggta tacatatcag tatatattct tataccgcaa aaatcagcgc
2101 gcaaatacgc atactgttat ctggctttta gtaagccgga tccagatctt tacgccccgc
2161 cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat
2221 cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat
2281 aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat
2341 caaaactggt gaaactcacc cagggattgg ctgagacgaa aaacatattc tcaataaacc
2401 ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta
2461 gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct
2521 catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca
2581 ttgccatacg gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg
2641 gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa
2701 cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat
2761 gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct
2821 tagctcctga aaatctcgac ggatcctaac tcaaaatcca cacattatac gagccggaag
2881 cataaagtgt aaagcctggg gtgcctaatg cggccgccat agtgactgga tatgttgtgt
2941 tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat
3001 atcattttac gtttctcgtt cagctttttt gtacaaactt gttgatatcg ctagccaatt
3061 gataacttcg tataggatac tttatacgaa gttatcattg ggattcttcc tattttgatc
3121 caagcatcac catcgaccct ctagtccaga tctcaccatc gacccataac ttcgtatagc
3181 atacattata cgaagttata tggtgagcaa gggcgattct agaaacttgt ttattgcagc
3241 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
3301 actgcattct agttgtggtt tgtccaaact catcaatgta tcttaaagct tggtgtggaa
3361 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
3421 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
3481 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
3541 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
3601 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggccatgacc
3661 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccctcgggc cgtacgcacc
3721 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtggaccc ggaccgccac
3781 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
3841 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
3901 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
3961 ctggccgcgc agcaacagat ggaagggctc ctggcgccgc accggcccaa ggagcccgcg
4021 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
4081 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
4141 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
4201 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgactgtgc
4261 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
4321 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
4381 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
4441 agaatagcag gcatgctggg gaaagcttag gaacccctag tgatggagtt ggccactccc
4501 tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc
4561 tttgcccggg cggcctcagt gagcgagcga gcgcgcaggg atcccggtgt gaaataccgc
4621 acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact
4681 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
4741 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
4801 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
4861 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
4921 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
4981 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
5041 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
5101 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
5161 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
5221 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
5281 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
5341 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
5401 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
5461 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
5521 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
5581 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
5641 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
5701 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
5761 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
5821 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
5881 ttaatagttt gcgcaacgtt gttgaaaaag gatcttcacc tagatccttt tcacgtagaa
5941 agccagtccg cagaaacggt gctgaccccg gatgaatgtc agctactggg ctatctggac
6001 aagggaaaac gcaagcgcaa agagaaagca ggtagcttgc agtgggctta catggcgata
6061 gctagactgg gcggttttat ggacagcaag cgaaccggaa ttgccagctg gggcgccctc
6121 tggtaaggtt gggaagccct gcaaagtaaa ctggatggct ttctcgccgc caaggatctg
6181 atggcgcagg ggatcaagct ctgatcaaga gacaggatga ggatcgtttc gcatgattga
6241 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
6301 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
6361 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga
6421 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
6481 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
6541 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
6601 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
6661 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
6721 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga
6781 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
6841 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
6901 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
6961 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
7021 cttctgaatt ttgttaaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
7081 tcggcaacat cccttataaa tcaaaagaat agaccgcgat agggttgagt gttgttccag
7141 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
7201 tctatcaggg cgatggccca ctacgtgaac catcacccaa atcaagtttt ttgcggtcga
7261 ggtgccgtaa agctctaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
7321 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
7381 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgcgcgc ttaatgcgcc
7441 gctacagggc gcgtccattc gccattcagg atcgaattaa ttcttaatta acatcatcaa
7501 taatatacct t
LOCUS dna 7511 bp
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
misc_binding 457..462
/dbxref="REBASE:HpaI"
misc_binding 833..838
/dbxref="REBASE:NdeI"
misc_binding 1168..1173
/dbxref="REBASE:KpnI"
Other Gene 1288..1321
/gene="loxP other"
misc_binding 1322..1327
/dbxref="REBASE:XhoI"
Other Gene 1343..1462
/gene="attR1 other"
Other Gene 1362..1462
/gene="attR2 other"
misc_binding 1469..1474
/dbxref="REBASE:PstI"
Other Gene 1503..1808
/gene="ccdB other"
Marker 2150..2809
/gene="CAT marker"
ORF 2150..2809
/sequence="ORF_3 rf(5)"
misc_binding 2591..2596
/dbxref="REBASE:EcoRI"
misc_binding 2910..2917
/dbxref="REBASE:NotI"
Other Gene 2918..3042
/gene="attR1 other"
Other Gene 2918..3018
/gene="attR2 other"
Other Gene 3166..3199
/gene="loxP other"
misc_binding 3218..3223
/dbxref="REBASE:XbaI"
Promoter 3352..3620
/gene="SV40 prom"
Rep_Origin 3519..3596
/gene="SV40 origin"
ORF 3586..4254
/sequence="ORF_1 rf(1)"
misc_binding 3599..3611
/dbxref="REBASE:SfiI"
Marker 3655..4254
/gene="puro marker"
Rep_Origin 4823..5442
/gene="pBR322 origin"
Promoter 6095..6144
/gene="NEOKAN prom"
misc_binding 6202..6207
/dbxref="REBASE:BclI"
ORF 6233..7027
/sequence="ORF_2 rf(2)"
Marker 6236..7024
/gene="NTP_II marker"
Rep_Origin 7132..7437
/gene="f1 origin"
BASE COUNT 1839 a 1949 c 1930 g 1793 t 0 others
ORIGIN
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta acctgcgcgc tcgctcgctc
481 actgaggccg cccgggcaaa gcccgggcgt cgggcgacct ttggtcgccc ggcctcagtg
541 agcgagcgag cgcgcagaga gggagtggcc aactccatca ctaggggttc cttgtagtta
601 atgattaacc cgccatgcta cttatctacg tagccatgct ctaggaagat cgcctaggcg
661 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
721 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
781 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
841 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
901 catgacctta tgggactttc ctacttggca gtacatctac gtttagtcat cgctattacc
961 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
1021 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
1081 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
1141 cggtgggagg tctatataag cagagctggt accaatcagc tcgctcacca tataacttcg
1201 tataaagtat cctatacgaa gttatatcaa aataggaaga ccaatgcttc accatcgacc
1261 cgaattgcca agcatcacca tcgacccata acttcgtata atgtatgcta tacgaagtta
1321 tctcgagtca gatatcaacc actttgtaca agaaagctga acgagaaacg taaaatgata
1381 taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa
1441 cacaacatat ccagtcacta tggtcgacct gcagactggc tgtgtataag ggagcctgac
1501 atttatattc cccagaacat caggttaatg gcgtttttga tgtcattttc gcggtggctg
1561 agatcagcca cttcttcccc gataacggag accggcacac tggccatatc ggtggtcatc
1621 atgcgccagc tttcatcccc gatatgcacc accgggtaaa gttcacggga gactttatct
1681 gacagcagac gtgcactggc cagggggatc accatccgtc gcccgggcgt gtcaataata
1741 tcactctgta catccacaaa cagacgataa cggctctctc ttttataggt gtaaacctta
1801 aactgcattt caccagcccc tgttctcgtc agcaaaagag ccgttcattt caataaaccg
1861 ggcgacctca gccatccctt cctgattttc cgctttccag cgttcggcac gcagacgacg
1921 ggcttcattc tgcatggttg tgcttaccag accggagata ttgacatcat atatgccttg
1981 agcaactgat agctgtcgct gtcaactgtc actgtaatac gctgcttcat agcatacctc
2041 tttttgacat acttcgggta tacatatcag tatatattct tataccgcaa aaatcagcgc
2101 gcaaatacgc atactgttat ctggctttta gtaagccgga tccagatctt tacgccccgc
2161 cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat
2221 cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat
2281 aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat
2341 caaaactggt gaaactcacc cagggattgg ctgagacgaa aaacatattc tcaataaacc
2401 ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta
2461 gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct
2521 catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca
2581 ttgccatacg gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg
2641 gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa
2701 cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat
2761 gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct
2821 tagctcctga aaatctcgac ggatcctaac tcaaaatcca cacattatac gagccggaag
2881 cataaagtgt aaagcctggg gtgcctaatg cggccgccat agtgactgga tatgttgtgt
2941 tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat
3001 atcattttac gtttctcgtt cagctttttt gtacaaactt gttgatatcg ctagccaatt
3061 gataacttcg tataggatac tttatacgaa gttatcattg ggattcttcc tattttgatc
3121 caagcatcac catcgaccct ctagtccaga tctcaccatc gacccataac ttcgtatagc
3181 atacattata cgaagttata tggtgagcaa gggcgattct agaaacttgt ttattgcagc
3241 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
3301 actgcattct agttgtggtt tgtccaaact catcaatgta tcttaaagct tggtgtggaa
3361 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
3421 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
3481 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
3541 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
3601 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggccatgacc
3661 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccctcgggc cgtacgcacc
3721 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtggaccc ggaccgccac
3781 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
3841 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
3901 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
3961 ctggccgcgc agcaacagat ggaagggctc ctggcgccgc accggcccaa ggagcccgcg
4021 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
4081 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
4141 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
4201 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgactgtgc
4261 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
4321 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
4381 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
4441 agaatagcag gcatgctggg gaaagcttag gaacccctag tgatggagtt ggccactccc
4501 tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc
4561 tttgcccggg cggcctcagt gagcgagcga gcgcgcaggg atcccggtgt gaaataccgc
4621 acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact
4681 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
4741 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
4801 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
4861 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
4921 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
4981 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
5041 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
5101 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
5161 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
5221 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
5281 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
5341 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
5401 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
5461 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
5521 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
5581 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
5641 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
5701 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
5761 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
5821 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
5881 ttaatagttt gcgcaacgtt gttgaaaaag gatcttcacc tagatccttt tcacgtagaa
5941 agccagtccg cagaaacggt gctgaccccg gatgaatgtc agctactggg ctatctggac
6001 aagggaaaac gcaagcgcaa agagaaagca ggtagcttgc agtgggctta catggcgata
6061 gctagactgg gcggttttat ggacagcaag cgaaccggaa ttgccagctg gggcgccctc
6121 tggtaaggtt gggaagccct gcaaagtaaa ctggatggct ttctcgccgc caaggatctg
6181 atggcgcagg ggatcaagct ctgatcaaga gacaggatga ggatcgtttc gcatgattga
6241 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
6301 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
6361 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga
6421 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
6481 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
6541 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
6601 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
6661 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
6721 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga
6781 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
6841 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
6901 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
6961 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
7021 cttctgaatt ttgttaaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
7081 tcggcaacat cccttataaa tcaaaagaat agaccgcgat agggttgagt gttgttccag
7141 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
7201 tctatcaggg cgatggccca ctacgtgaac catcacccaa atcaagtttt ttgcggtcga
7261 ggtgccgtaa agctctaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
7321 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
7381 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgcgcgc ttaatgcgcc
7441 gctacagggc gcgtccattc gccattcagg atcgaattaa ttcttaatta acatcatcaa
7501 taatatacct t
//
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 ttttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgt 60
AAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCA
61 gggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaa 120
CCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTT
Encap other(162,310)>>>
|
121 cacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgccggtgtacacagg 180
GTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCC
181 aagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagt 240
TTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCA
241 aagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttg 300
TTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAAC
301 tgttactcatagcgcgtaatacggcagacctcagcgctagattattgaagcatttatcag 360
ACAATGAGTATCGCGCATTATGCCGTCTGGAGTCGCGATCTAATAACTTCGTAAATAGTC
amp prom(365,393)<<<
|
361 ggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggg 420
CCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCC
HpaI
|
421 gttccgcgcacatttccccgaaaagtgccacctgacgttaacctgcgcgctcgctcgctc 480
CAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAATTGGACGCGCGAGCGAGCGAG
481 actgaggccgcccgggcaaagcccgggcgtcgggcgacctttggtcgcccggcctcagtg 540
TGACTCCGGCGGGCCCGTTTCGGGCCCGCAGCCCGCTGGAAACCAGCGGGCCGGAGTCAC
541 agcgagcgagcgcgcagagagggagtggccaactccatcactaggggttccttgtagtta 600
TCGCTCGCTCGCGCGTCTCTCCCTCACCGGTTGAGGTAGTGATCCCCAAGGAACATCAAT
601 atgattaacccgccatgctacttatctacgtagccatgctctaggaagatcgcctaggcg 660
TACTAATTGGGCGGTACGATGAATAGATGCATCGGTACGAGATCCTTCTAGCGGATCCGC
661 cgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccatt 720
GCAATGTATTGAATGCCATTTACCGGGCGGACCGACTGGCGGGTTGCTGGGGGCGGGTAA
721 gacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtca 780
CTGCAGTTATTACTGCATACAAGGGTATCATTGCGGTTATCCCTGAAAGGTAACTGCAGT
NdeI
|
781 atgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgcc 840
TACCCACCTCATAAATGCCATTTGACGGGTGAACCGTCATGTAGTTCACATAGTATACGG
841 aagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagta 900
TTCATGCGGGGGATAACTGCAGTTACTGCCATTTACCGGGCGGACCGTAATACGGGTCAT
901 catgaccttatgggactttcctacttggcagtacatctacgtttagtcatcgctattacc 960
GTACTGGAATACCCTGAAAGGATGAACCGTCATGTAGATGCAAATCAGTAGCGATAATGG
961 atggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacgggga 1020
TACCACTACGCCAAAACCGTCATGTAGTTACCCGCACCTATCGCCAAACTGAGTGCCCCT
1021 tttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgg 1080
AAAGGTTCAGAGGTGGGGTAACTGCAGTTACCCTCAAACAAAACCGTGGTTTTAGTTGCC
1081 gactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgta 1140
CTGAAAGGTTTTACAGCATTGTTGAGGCGGGGTAACTGCGTTTACCCGCCATCCGCACAT
KpnI
|
1141 cggtgggaggtctatataagcagagctggtaccaatcagctcgctcaccatataacttcg 1200
GCCACCCTCCAGATATATTCGTCTCGACCATGGTTAGTCGAGCGAGTGGTATATTGAAGC
1201 tataaagtatcctatacgaagttatatcaaaataggaagaccaatgcttcaccatcgacc 1260
ATATTTCATAGGATATGCTTCAATATAGTTTTATCCTTCTGGTTACGAAGTGGTAGCTGG
loxP other(1288,1321)<<<
|
1261 cgaattgccaagcatcaccatcgacccataacttcgtataatgtatgctatacgaagtta 1320
GCTTAACGGTTCGTAGTGGTAGCTGGGTATTGAAGCATATTACATACGATATGCTTCAAT
attR2 other(1362,1462)<<<
|
XhoI attR1 other(1343,1462)>>>
| | |
1321 tctcgagtcagatatcaaccactttgtacaagaaagctgaacgagaaacgtaaaatgata 1380
AGAGCTCAGTCTATAGTTGGTGAAACATGTTCTTTCGACTTGCTCTTTGCATTTTACTAT
1381 taaatatcaatatattaaattagattttgcataaaaaacagactacataatactgtaaaa 1440
ATTTATAGTTATATAATTTAATCTAAAACGTATTTTTTGTCTGATGTATTATGACATTTT
PstI
|
1441 cacaacatatccagtcactatggtcgacctgcagactggctgtgtataagggagcctgac 1500
GTGTTGTATAGGTCAGTGATACCAGCTGGACGTCTGACCGACACATATTCCCTCGGACTG
ccdB other(1503,1808)<<<
|
1501 atttatattccccagaacatcaggttaatggcgtttttgatgtcattttcgcggtggctg 1560
TAAATATAAGGGGTCTTGTAGTCCAATTACCGCAAAAACTACAGTAAAAGCGCCACCGAC
1561 agatcagccacttcttccccgataacggagaccggcacactggccatatcggtggtcatc 1620
TCTAGTCGGTGAAGAAGGGGCTATTGCCTCTGGCCGTGTGACCGGTATAGCCACCAGTAG
1621 atgcgccagctttcatccccgatatgcaccaccgggtaaagttcacgggagactttatct 1680
TACGCGGTCGAAAGTAGGGGCTATACGTGGTGGCCCATTTCAAGTGCCCTCTGAAATAGA
1681 gacagcagacgtgcactggccagggggatcaccatccgtcgcccgggcgtgtcaataata 1740
CTGTCGTCTGCACGTGACCGGTCCCCCTAGTGGTAGGCAGCGGGCCCGCACAGTTATTAT
1741 tcactctgtacatccacaaacagacgataacggctctctcttttataggtgtaaacctta 1800
AGTGAGACATGTAGGTGTTTGTCTGCTATTGCCGAGAGAGAAAATATCCACATTTGGAAT
1801 aactgcatttcaccagcccctgttctcgtcagcaaaagagccgttcatttcaataaaccg 1860
TTGACGTAAAGTGGTCGGGGACAAGAGCAGTCGTTTTCTCGGCAAGTAAAGTTATTTGGC
1861 ggcgacctcagccatcccttcctgattttccgctttccagcgttcggcacgcagacgacg 1920
CCGCTGGAGTCGGTAGGGAAGGACTAAAAGGCGAAAGGTCGCAAGCCGTGCGTCTGCTGC
1921 ggcttcattctgcatggttgtgcttaccagaccggagatattgacatcatatatgccttg 1980
CCGAAGTAAGACGTACCAACACGAATGGTCTGGCCTCTATAACTGTAGTATATACGGAAC
1981 agcaactgatagctgtcgctgtcaactgtcactgtaatacgctgcttcatagcatacctc 2040
TCGTTGACTATCGACAGCGACAGTTGACAGTGACATTATGCGACGAAGTATCGTATGGAG
2041 tttttgacatacttcgggtatacatatcagtatatattcttataccgcaaaaatcagcgc 2100
AAAAACTGTATGAAGCCCATATGTATAGTCATATATAAGAATATGGCGTTTTTAGTCGCG
ORF_3 rf(5)(2150,2809)<<<
|
CAT marker(2150,2809)<<<
|
2101 gcaaatacgcatactgttatctggcttttagtaagccggatccagatctttacgccccgc 2160
CGTTTATGCGTATGACAATAGACCGAAAATCATTCGGCCTAGGTCTAGAAATGCGGGGCG
2161 cctgccactcatcgcagtactgttgtaattcattaagcattctgccgacatggaagccat 2220
GGACGGTGAGTAGCGTCATGACAACATTAAGTAATTCGTAAGACGGCTGTACCTTCGGTA
2221 cacaaacggcatgatgaacctgaatcgccagcggcatcagcaccttgtcgccttgcgtat 2280
GTGTTTGCCGTACTACTTGGACTTAGCGGTCGCCGTAGTCGTGGAACAGCGGAACGCATA
2281 aatatttgcccatggtgaaaacgggggcgaagaagttgtccatattggccacgtttaaat 2340
TTATAAACGGGTACCACTTTTGCCCCCGCTTCTTCAACAGGTATAACCGGTGCAAATTTA
2341 caaaactggtgaaactcacccagggattggctgagacgaaaaacatattctcaataaacc 2400
GTTTTGACCACTTTGAGTGGGTCCCTAACCGACTCTGCTTTTTGTATAAGAGTTATTTGG
2401 ctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtgta 2460
GAAATCCCTTTATCCGGTCCAAAAGTGGCATTGTGCGGTGTAGAACGCTTATATACACAT
2461 gaaactgccggaaatcgtcgtggtattcactccagagcgatgaaaacgtttcagtttgct 2520
CTTTGACGGCCTTTAGCAGCACCATAAGTGAGGTCTCGCTACTTTTGCAAAGTCAAACGA
2521 catggaaaacggtgtaacaagggtgaacactatcccatatcaccagctcaccgtctttca 2580
GTACCTTTTGCCACATTGTTCCCACTTGTGATAGGGTATAGTGGTCGAGTGGCAGAAAGT
EcoRI
|
2581 ttgccatacggaattccggatgagcattcatcaggcgggcaagaatgtgaataaaggccg 2640
AACGGTATGCCTTAAGGCCTACTCGTAAGTAGTCCGCCCGTTCTTACACTTATTTCCGGC
2641 gataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaatatccagctgaa 2700
CTATTTTGAACACGAATAAAAAGAAATGCCAGAAATTTTTCCGGCATTATAGGTCGACTT
2701 cggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctttacgat 2760
GCCAGACCAATATCCATGTAACTCGTTGACTGACTTTACGGAGTTTTACAAGAAATGCTA
2761 gccattgggatatatcaacggtggtatatccagtgatttttttctccattttagcttcct 2820
CGGTAACCCTATATAGTTGCCACCATATAGGTCACTAAAAAAAGAGGTAAAATCGAAGGA
2821 tagctcctgaaaatctcgacggatcctaactcaaaatccacacattatacgagccggaag 2880
ATCGAGGACTTTTAGAGCTGCCTAGGATTGAGTTTTAGGTGTGTAATATGCTCGGCCTTC
attR1 other(2918,3042)<<<
|
NotI attR2 other(2918,3018)>>>
| |
2881 cataaagtgtaaagcctggggtgcctaatgcggccgccatagtgactggatatgttgtgt 2940
GTATTTCACATTTCGGACCCCACGGATTACGCCGGCGGTATCACTGACCTATACAACACA
2941 tttacagtattatgtagtctgttttttatgcaaaatctaatttaatatattgatatttat 3000
AAATGTCATAATACATCAGACAAAAAATACGTTTTAGATTAAATTATATAACTATAAATA
3001 atcattttacgtttctcgttcagcttttttgtacaaacttgttgatatcgctagccaatt 3060
TAGTAAAATGCAAAGAGCAAGTCGAAAAAACATGTTTGAACAACTATAGCGATCGGTTAA
3061 gataacttcgtataggatactttatacgaagttatcattgggattcttcctattttgatc 3120
CTATTGAAGCATATCCTATGAAATATGCTTCAATAGTAACCCTAAGAAGGATAAAACTAG
loxP other(3166,3199)>>>
|
3121 caagcatcaccatcgaccctctagtccagatctcaccatcgacccataacttcgtatagc 3180
GTTCGTAGTGGTAGCTGGGAGATCAGGTCTAGAGTGGTAGCTGGGTATTGAAGCATATCG
XbaI
|
3181 atacattatacgaagttatatggtgagcaagggcgattctagaaacttgtttattgcagc 3240
TATGTAATATGCTTCAATATACCACTCGTTCCCGCTAAGATCTTTGAACAAATAACGTCG
3241 ttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttc 3300
AATATTACCAATGTTTATTTCGTTATCGTAGTGTTTAAAGTGTTTATTTCGTAAAAAAAG
SV40 prom(3352,3620)>>>
|
3301 actgcattctagttgtggtttgtccaaactcatcaatgtatcttaaagcttggtgtggaa 3360
TGACGTAAGATCAACACCAAACAGGTTTGAGTAGTTACATAGAATTTCGAACCACACCTT
3361 agtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaa 3420
TCAGGGGTCCGAGGGGTCGTCCGTCTTCATACGTTTCGTACGTAGAGTTAATCAGTCGTT
3421 ccaggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctca 3480
GGTCCACACCTTTCAGGGGTCCGAGGGGTCGTCCGTCTTCATACGTTTCGTACGTAGAGT
SV40 origin(3519,3596)>>>
|
3481 attagtcagcaaccatagtcccgcccctaactccgcccatcccgcccctaactccgccca 3540
TAATCAGTCGTTGGTATCAGGGCGGGGATTGAGGCGGGTAGGGCGGGGATTGAGGCGGGT
ORF_1 rf(1)(3586,4254)>>>
|
3541 gttccgcccattctccgccccatggctgactaattttttttatttatgcagaggccgagg 3600
CAAGGCGGGTAAGAGGCGGGGTACCGACTGATTAAAAAAAATAAATACGTCTCCGGCTCC
SfiI puro marker(3655,4254)>>>
| |
3601 ccgcctcggcctctgagctattccagaagtagtgaggaggcttttttggaggccatgacc 3660
GGCGGAGCCGGAGACTCGATAAGGTCTTCATCACTCCTCCGAAAAAACCTCCGGTACTGG
3661 gagtacaagcccacggtgcgcctcgccacccgcgacgacgtccctcgggccgtacgcacc 3720
CTCATGTTCGGGTGCCACGCGGAGCGGTGGGCGCTGCTGCAGGGAGCCCGGCATGCGTGG
3721 ctcgccgccgcgttcgccgactaccccgccacgcgccacaccgtggacccggaccgccac 3780
GAGCGGCGGCGCAAGCGGCTGATGGGGCGGTGCGCGGTGTGGCACCTGGGCCTGGCGGTG
3781 atcgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggctcgacatcggc 3840
TAGCTCGCCCAGTGGCTCGACGTTCTTGAGAAGGAGTGCGCGCAGCCCGAGCTGTAGCCG
3841 aaggtgtgggtcgcggacgacggcgccgcggtggcggtctggaccacgccggagagcgtc 3900
TTCCACACCCAGCGCCTGCTGCCGCGGCGCCACCGCCAGACCTGGTGCGGCCTCTCGCAG
3901 gaagcgggggcggtgttcgccgagatcggcccgcgcatggccgagttgagcggttcccgg 3960
CTTCGCCCCCGCCACAAGCGGCTCTAGCCGGGCGCGTACCGGCTCAACTCGCCAAGGGCC
3961 ctggccgcgcagcaacagatggaagggctcctggcgccgcaccggcccaaggagcccgcg 4020
GACCGGCGCGTCGTTGTCTACCTTCCCGAGGACCGCGGCGTGGCCGGGTTCCTCGGGCGC
4021 tggttcctggccaccgtcggcgtctcgcccgaccaccagggcaagggtctgggcagcgcc 4080
ACCAAGGACCGGTGGCAGCCGCAGAGCGGGCTGGTGGTCCCGTTCCCAGACCCGTCGCGG
4081 gtcgtgctccccggagtggaggcggccgagcgcgccggggtgcccgccttcctggagacc 4140
CAGCACGAGGGGCCTCACCTCCGCCGGCTCGCGCGGCCCCACGGGCGGAAGGACCTCTGG
4141 tccgcgccccgcaacctccccttctacgagcggctcggcttcaccgtcaccgccgacgtc 4200
AGGCGCGGGGCGTTGGAGGGGAAGATGCTCGCCGAGCCGAAGTGGCAGTGGCGGCTGCAG
4201 gaggtgcccgaaggaccgcgcacctggtgcatgacccgcaagcccggtgcctgactgtgc 4260
CTCCACGGGCTTCCTGGCGCGTGGACCACGTACTGGGCGTTCGGGCCACGGACTGACACG
4261 cttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaag 4320
GAAGATCAACGGTCGGTAGACAACAAACGGGGAGGGGGCACGGAAGGAACTGGGACCTTC
4321 gtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagta 4380
CACGGTGAGGGTGACAGGAAAGGATTATTTTACTCCTTTAACGTAGCGTAACAGACTCAT
4381 ggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaag 4440
CCACAGTAAGATAAGACCCCCCACCCCACCCCGTCCTGTCGTTCCCCCTCCTAACCCTTC
4441 agaatagcaggcatgctggggaaagcttaggaacccctagtgatggagttggccactccc 4500
TCTTATCGTCCGTACGACCCCTTTCGAATCCTTGGGGATCACTACCTCAACCGGTGAGGG
4501 tctctgcgcgctcgctcgctcactgaggccgggcgaccaaaggtcgcccgacgcccgggc 4560
AGAGACGCGCGAGCGAGCGAGTGACTCCGGCCCGCTGGTTTCCAGCGGGCTGCGGGCCCG
4561 tttgcccgggcggcctcagtgagcgagcgagcgcgcagggatcccggtgtgaaataccgc 4620
AAACGGGCCCGCCGGAGTCACTCGCTCGCTCGCGCGTCCCTAGGGCCACACTTTATGGCG
4621 acagatgcgtaaggagaaaataccgcatcaggcgctcttccgcttcctcgctcactgact 4680
TGTCTACGCATTCCTCTTTTATGGCGTAGTCCGCGAGAAGGCGAAGGAGCGAGTGACTGA
4681 cgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatac 4740
GCGACGCGAGCCAGCAAGCCGACGCCGCTCGCCATAGTCGAGTGAGTTTCCGCCATTATG
4741 ggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaa 4800
CCAATAGGTGTCTTAGTCCCCTATTGCGTCCTTTCTTGTACACTCGTTTTCCGGTCGTTT
pBR322 origin(4823,5442)<<<
|
4801 aggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctg 4860
TCCGGTCCTTGGCATTTTTCCGGCGCAACGACCGCAAAAAGGTATCCGAGGCGGGGGGAC
4861 acgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaa 4920
TGCTCGTAGTGTTTTTAGCTGCGAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGATATTT
4921 gataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgc 4980
CTATGGTCCGCAAAGGGGGACCTTCGAGGGAGCACGCGAGAGGACAAGGCTGGGACGGCG
4981 ttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcac 5040
AATGGCCTATGGACAGGCGGAAAGAGGGAAGCCCTTCGCACCGCGAAAGAGTATCGAGTG
5041 gctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaac 5100
CGACATCCATAGAGTCAAGCCACATCCAGCAAGCGAGGTTCGACCCGACACACGTGCTTG
5101 cccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccgg 5160
GGGGGCAAGTCGGGCTGGCGACGCGGAATAGGCCATTGATAGCAGAACTCAGGTTGGGCC
5161 taagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggt 5220
ATTCTGTGCTGAATAGCGGTGACCGTCGTCGGTGACCATTGTCCTAATCGTCTCGCTCCA
5221 atgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagga 5280
TACATCCGCCACGATGTCTCAAGAACTTCACCACCGGATTGATGCCGATGTGATCTTCCT
5281 cagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagct 5340
GTCATAAACCATAGACGCGAGACGACTTCGGTCAATGGAAGCCTTTTTCTCAACCATCGA
5341 cttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcaga 5400
GAACTAGGCCGTTTGTTTGGTGGCGACCATCGCCACCAAAAAAACAAACGTTCGTCGTCT
5401 ttacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacg 5460
AATGCGCGTCTTTTTTTCCTAGAGTTCTTCTAGGAAACTAGAAAAGATGCCCCAGACTGC
5461 ctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatct 5520
GAGTCACCTTGCTTTTGAGTGCAATTCCCTAAAACCAGTACTCTAATAGTTTTTCCTAGA
5521 tcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagt 5580
AGTGGATCTAGGAAAATTTAATTTTTACTTCAAAATTTAGTTAGATTTCATATATACTCA
5581 aaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtc 5640
TTTGAACCAGACTGTCAATGGTTACGAATTAGTCACTCCGTGGATAGAGTCGCTAGACAG
5641 tatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagg 5700
ATAAAGCAAGTAGGTATCAACGGACTGAGGGGCAGCACATCTATTGATGCTATGCCCTCC
5701 gcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccag 5760
CGAATGGTAGACCGGGGTCACGACGTTACTATGGCGCTCTGGGTGCGAGTGGCCGAGGTC
5761 atttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactt 5820
TAAATAGTCGTTATTTGGTCGGTCGGCCTTCCCGGCTCGCGTCTTCACCAGGACGTTGAA
5821 tatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccag 5880
ATAGGCGGAGGTAGGTCAGATAATTAACAACGGCCCTTCGATCTCATTCATCAAGCGGTC
5881 ttaatagtttgcgcaacgttgttgaaaaaggatcttcacctagatccttttcacgtagaa 5940
AATTATCAAACGCGTTGCAACAACTTTTTCCTAGAAGTGGATCTAGGAAAAGTGCATCTT
5941 agccagtccgcagaaacggtgctgaccccggatgaatgtcagctactgggctatctggac 6000
TCGGTCAGGCGTCTTTGCCACGACTGGGGCCTACTTACAGTCGATGACCCGATAGACCTG
6001 aagggaaaacgcaagcgcaaagagaaagcaggtagcttgcagtgggcttacatggcgata 6060
TTCCCTTTTGCGTTCGCGTTTCTCTTTCGTCCATCGAACGTCACCCGAATGTACCGCTAT
NEOKAN prom(6095,6144)>>>
|
6061 gctagactgggcggttttatggacagcaagcgaaccggaattgccagctggggcgccctc 6120
CGATCTGACCCGCCAAAATACCTGTCGTTCGCTTGGCCTTAACGGTCGACCCCGCGGGAG
6121 tggtaaggttgggaagccctgcaaagtaaactggatggctttctcgccgccaaggatctg 6180
ACCATTCCAACCCTTCGGGACGTTTCATTTGACCTACCGAAAGAGCGGCGGTTCCTAGAC
NTP_II marker(6236,7024)>>>
|
BclI ORF_2 rf(2)(6233,7027)>>>
| | |
6181 atggcgcaggggatcaagctctgatcaagagacaggatgaggatcgtttcgcatgattga 6240
TACCGCGTCCCCTAGTTCGAGACTAGTTCTCTGTCCTACTCCTAGCAAAGCGTACTAACT
6241 acaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggctatga 6300
TGTTCTACCTAACGTGCGTCCAAGAGGCCGGCGAACCCACCTCTCCGATAAGCCGATACT
6301 ctgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggg 6360
GACCCGTGTTGTCTGTTAGCCGACGAGACTACGGCGGCACAAGGCCGACAGTCGCGTCCC
6361 gcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaagacga 6420
CGCGGGCCAAGAAAAACAGTTCTGGCTGGACAGGCCACGGGACTTACTTGACGTTCTGCT
6421 ggcagcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgt 6480
CCGTCGCGCCGATAGCACCGACCGGTGCTGCCCGCAAGGAACGCGTCGACACGAGCTGCA
6481 tgtcactgaagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcct 6540
ACAGTGACTTCGCCCTTCCCTGACCGACGATAACCCGCTTCACGGCCCCGTCCTAGAGGA
6541 gtcatctcaccttgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggct 6600
CAGTAGAGTGGAACGAGGACGGCTCTTTCATAGGTAGTACCGACTACGTTACGCCGCCGA
6601 gcatacgcttgatccggctacctgcccattcgaccaccaagcgaaacatcgcatcgagcg 6660
CGTATGCGAACTAGGCCGATGGACGGGTAAGCTGGTGGTTCGCTTTGTAGCGTAGCTCGC
6661 agcacgtactcggatggaagccggtcttgtcgatcaggatgatctggacgaagagcatca 6720
TCGTGCATGAGCCTACCTTCGGCCAGAACAGCTAGTCCTACTAGACCTGCTTCTCGTAGT
6721 ggggctcgcgccagccgaactgttcgccaggctcaaggcgagcatgcccgacggcgagga 6780
CCCCGAGCGCGGTCGGCTTGACAAGCGGTCCGAGTTCCGCTCGTACGGGCTGCCGCTCCT
6781 tctcgtcgtgacccatggcgatgcctgcttgccgaatatcatggtggaaaatggccgctt 6840
AGAGCAGCACTGGGTACCGCTACGGACGAACGGCTTATAGTACCACCTTTTACCGGCGAA
6841 ttctggattcatcgactgtggccggctgggtgtggcggaccgctatcaggacatagcgtt 6900
AAGACCTAAGTAGCTGACACCGGCCGACCCACACCGCCTGGCGATAGTCCTGTATCGCAA
6901 ggctacccgtgatattgctgaagagcttggcggcgaatgggctgaccgcttcctcgtgct 6960
CCGATGGGCACTATAACGACTTCTCGAACCGCCGCTTACCCGACTGGCGAAGGAGCACGA
6961 ttacggtatcgccgctcccgattcgcagcgcatcgccttctatcgccttcttgacgagtt 7020
AATGCCATAGCGGCGAGGGCTAAGCGTCGCGTAGCGGAAGATAGCGGAAGAACTGCTCAA
7021 cttctgaattttgttaaaatttttgttaaatcagctcattttttaaccaataggccgaaa 7080
GAAGACTTAAAACAATTTTAAAAACAATTTAGTCGAGTAAAAAATTGGTTATCCGGCTTT
f1 origin(7132,7437)<<<
|
7081 tcggcaacatcccttataaatcaaaagaatagaccgcgatagggttgagtgttgttccag 7140
AGCCGTTGTAGGGAATATTTAGTTTTCTTATCTGGCGCTATCCCAACTCACAACAAGGTC
7141 tttggaacaagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaaaccg 7200
AAACCTTGTTCTCAGGTGATAATTTCTTGCACCTGAGGTTGCAGTTTCCCGCTTTTTGGC
7201 tctatcagggcgatggcccactacgtgaaccatcacccaaatcaagttttttgcggtcga 7260
AGATAGTCCCGCTACCGGGTGATGCACTTGGTAGTGGGTTTAGTTCAAAAAACGCCAGCT
7261 ggtgccgtaaagctctaaatcggaaccctaaagggagcccccgatttagagcttgacggg 7320
CCACGGCATTTCGAGATTTAGCCTTGGGATTTCCCTCGGGGGCTAAATCTCGAACTGCCC
7321 gaaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgctaggg 7380
CTTTCGGCCGCTTGCACCGCTCTTTCCTTCCCTTCTTTCGCTTTCCTCGCCCGCGATCCC
7381 cgctggcaagtgtagcggtcacgctgcgcgtaaccaccacacccgcgcgcttaatgcgcc 7440
GCGACCGTTCACATCGCCAGTGCGACGCGCATTGGTGGTGTGGGCGCGCGAATTACGCGG
7441 gctacagggcgcgtccattcgccattcaggatcgaattaattcttaattaacatcatcaa 7500
CGATGTCCCGCGCAGGTAAGCGGTAAGTCCTAGCTTAATTAAGAATTAATTGTAGTAGTT
7501 taatatacctt 7511
ATTATATGGAA
LOCUS dna 7511 bp
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
misc_binding 457..462
/dbxref="REBASE:HpaI"
misc_binding 833..838
/dbxref="REBASE:NdeI"
misc_binding 1168..1173
/dbxref="REBASE:KpnI"
Other Gene 1288..1321
/gene="loxP other"
misc_binding 1322..1327
/dbxref="REBASE:XhoI"
Other Gene 1343..1462
/gene="attR1 other"
Other Gene 1362..1462
/gene="attR2 other"
misc_binding 1469..1474
/dbxref="REBASE:PstI"
Other Gene 1503..1808
/gene="ccdB other"
Marker 2150..2809
/gene="CAT marker"
ORF 2150..2809
/sequence="ORF_3 rf(5)"
misc_binding 2591..2596
/dbxref="REBASE:EcoRI"
misc_binding 2910..2917
/dbxref="REBASE:NotI"
Other Gene 2918..3042
/gene="attR1 other"
Other Gene 2918..3018
/gene="attR2 other"
Other Gene 3166..3199
/gene="loxP other"
misc_binding 3218..3223
/dbxref="REBASE:XbaI"
Promoter 3352..3620
/gene="SV40 prom"
Rep_Origin 3519..3596
/gene="SV40 origin"
ORF 3586..4254
/sequence="ORF_1 rf(1)"
misc_binding 3599..3611
/dbxref="REBASE:SfiI"
Marker 3655..4254
/gene="puro marker"
Rep_Origin 4823..5442
/gene="pBR322 origin"
Promoter 6095..6144
/gene="NEOKAN prom"
misc_binding 6202..6207
/dbxref="REBASE:BclI"
ORF 6233..7027
/sequence="ORF_2 rf(2)"
Marker 6236..7024
/gene="NTP_II marker"
Rep_Origin 7132..7437
/gene="f1 origin"
BASE COUNT 1839 a 1949 c 1930 g 1793 t 0 others