pLenti-CMV-DIO-Blank-SV40-Puro
| Vector Name | pLenti-CMV-DIO-Blank-SV40-Puro |
| VectorType | Lentiviral Vector |
| Antibiotic Information | Bacterial: KanamycinMammalian: Puromycin |
| Sequencing Primers | CMV sequencing primer 5`---CGC AAA TGG GCG GTA GGC GTG---3` |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta
481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag
1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga
2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag
2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa
2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat
2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag
3061 agctagcccc gggatcgatc aattgagtac ttacgtaggt accaatcagc tcgctcacca
3121 tataacttcg tataaagtat cctatacgaa gttatatcaa aataggaaga ccaatgcttc
3181 accatcgacc cgaattgcca agcatcacca tcgacccata acttcgtata atgtatgcta
3241 tacgaagtta tctcgagtca gatatcgcta gccaattgat aacttcgtat aggatacttt
3301 atacgaagtt atcattggga ttcttcctat tttgatccaa gcatcaccat cgaccctcta
3361 gtccagatct caccatcgac ccataacttc gtatagcata cattatacga agttatatgg
3421 tgagcaaggg cgattctaga ctgcagctcg agtacccata cgacgtccca gactacgctt
3481 gagtttaaac acgcgtggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
3541 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
3601 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
3661 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
3721 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
3781 ggaggctttt ttggaggcca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
3841 cgacgtccct cgggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
3901 ccacaccgtg gacccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
3961 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
4021 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
4081 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag ggctcctggc
4141 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
4201 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
4261 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
4321 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
4381 ccgcaagccc ggtgcctgaa cgcgttccgg aaatcaacct ctggattaca aaatttgtga
4441 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
4501 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
4561 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
4621 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
4681 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
4741 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
4801 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg
4861 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
4921 gctgccggct ctgcggcctc ttccgcgtct cgccttcgcc ctcagacgag tcggatctcc
4981 ctttgggccg cctccccgcc tgtccggatg gaagggctaa ttcactccca acgaatacaa
5041 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
5101 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
5161 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
5221 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
5281 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt
5341 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
5401 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggcatct atgtcgggtg
5461 cggagaaaga ggtaatgaaa tggcattatg ggtattatgg gtctgcatta atgaatcggc
5521 caacgatccc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc
5581 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
5641 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
5701 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
5761 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
5821 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
5881 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
5941 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
6001 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
6061 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
6121 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
6181 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
6241 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
6301 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
6361 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
6421 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
6481 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
6541 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
6601 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
6661 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
6721 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
6781 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttga aaaaggatct
6841 tcacctagat ccttttcacg tagaaagcca gtccgcagaa acggtgctga ccccggatga
6901 atgtcagcta ctgggctatc tggacaaggg aaaacgcaag cgcaaagaga aagcaggtag
6961 cttgcagtgg gcttacatgg cgatagctag actgggcggt tttatggaca gcaagcgaac
7021 cggaattgcc agctggggcg ccctctggta aggttgggaa gccctgcaaa gtaaactgga
7081 tggctttctc gccgccaagg atctgatggc gcaggggatc aagctctgat caagagacag
7141 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
7201 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
7261 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
7321 gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg
7381 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
7441 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
7501 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
7561 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
7621 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
7681 aggcgagcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
7741 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
7801 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
7861 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
7921 ccttctatcg ccttcttgac gagttcttct gaattttgtt aaaatttttg ttaaatcagc
7981 tcatttttta accaataggc cgaaatcggc aacatccctt ataaatcaaa agaatagacc
8041 gcgatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
8101 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
8161 cccaaatcaa gttttttgcg gtcgaggtgc cgtaaagctc taaatcggaa ccctaaaggg
8221 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
8281 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
8341 accacacccg cgcgcttaat gcgccgctac agggcgcgtc cattcgccat tcaggatcga
8401 attaattctt aattaacatc atcaataata tacctt
LOCUS dna 8436 bp
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
misc_binding 457..462
/dbxref="REBASE:HpaI"
misc_binding 457..462
/dbxref="REBASE:HincII"
Other Gene 715..895
/gene="HIV-1_5_LTR other"
Other Gene 1006..1050
/gene="HIV-1_psi_pack other"
misc_binding 1092..1097
/dbxref="REBASE:NruI"
Regulatory_Seq 1557..1790
/gene="RRE reg"
ORF 1600..2301
/sequence="ORF_1 rf(1)"
misc_binding 2611..2616
/dbxref="REBASE:NdeI"
Promoter 2885..2966
/gene="CMV prom"
Regulatory_Seq 3022..3061
/gene="tetO reg"
misc_binding 3068..3073
/dbxref="REBASE:SmaI"
misc_binding 3068..3073
/dbxref="REBASE:XmaI"
misc_binding 3074..3079
/dbxref="REBASE:ClaI"
misc_binding 3098..3103
/dbxref="REBASE:KpnI"
Other Gene 3218..3251
/gene="loxP other"
misc_binding 3261..3266
/dbxref="REBASE:EcoRV"
Other Gene 3383..3416
/gene="loxP other"
misc_binding 3435..3440
/dbxref="REBASE:XbaI"
misc_binding 3441..3446
/dbxref="REBASE:PstI"
Promoter 3497..3765
/gene="SV40 prom"
Rep_Origin 3664..3741
/gene="SV40 origin"
ORF 3731..4399
/sequence="ORF_2 rf(2)"
misc_binding 3744..3756
/dbxref="REBASE:SfiI"
Marker 3800..4399
/gene="puro marker"
Other Gene 5009..5061
/gene="delta_U3 other"
Other Gene 5062..5242
/gene="HIV-1_5_LTR other"
Rep_Origin 5748..6367
/gene="pBR322 origin"
Promoter 7020..7069
/gene="NEOKAN prom"
misc_binding 7127..7132
/dbxref="REBASE:BclI"
ORF 7158..7952
/sequence="ORF_3 rf(3)"
Marker 7161..7949
/gene="NTP_II marker"
Rep_Origin 8057..8362
/gene="f1 origin"
BASE COUNT 2149 a 2071 c 2238 g 1978 t 0 others
ORIGIN
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta
481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag
1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga
2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag
2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa
2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat
2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag
3061 agctagcccc gggatcgatc aattgagtac ttacgtaggt accaatcagc tcgctcacca
3121 tataacttcg tataaagtat cctatacgaa gttatatcaa aataggaaga ccaatgcttc
3181 accatcgacc cgaattgcca agcatcacca tcgacccata acttcgtata atgtatgcta
3241 tacgaagtta tctcgagtca gatatcgcta gccaattgat aacttcgtat aggatacttt
3301 atacgaagtt atcattggga ttcttcctat tttgatccaa gcatcaccat cgaccctcta
3361 gtccagatct caccatcgac ccataacttc gtatagcata cattatacga agttatatgg
3421 tgagcaaggg cgattctaga ctgcagctcg agtacccata cgacgtccca gactacgctt
3481 gagtttaaac acgcgtggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
3541 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
3601 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
3661 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
3721 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
3781 ggaggctttt ttggaggcca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
3841 cgacgtccct cgggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
3901 ccacaccgtg gacccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
3961 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
4021 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
4081 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag ggctcctggc
4141 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
4201 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
4261 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
4321 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
4381 ccgcaagccc ggtgcctgaa cgcgttccgg aaatcaacct ctggattaca aaatttgtga
4441 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
4501 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
4561 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
4621 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
4681 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
4741 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
4801 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg
4861 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
4921 gctgccggct ctgcggcctc ttccgcgtct cgccttcgcc ctcagacgag tcggatctcc
4981 ctttgggccg cctccccgcc tgtccggatg gaagggctaa ttcactccca acgaatacaa
5041 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
5101 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
5161 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
5221 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
5281 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt
5341 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
5401 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggcatct atgtcgggtg
5461 cggagaaaga ggtaatgaaa tggcattatg ggtattatgg gtctgcatta atgaatcggc
5521 caacgatccc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc
5581 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
5641 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
5701 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
5761 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
5821 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
5881 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
5941 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
6001 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
6061 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
6121 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
6181 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
6241 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
6301 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
6361 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
6421 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
6481 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
6541 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
6601 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
6661 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
6721 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
6781 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttga aaaaggatct
6841 tcacctagat ccttttcacg tagaaagcca gtccgcagaa acggtgctga ccccggatga
6901 atgtcagcta ctgggctatc tggacaaggg aaaacgcaag cgcaaagaga aagcaggtag
6961 cttgcagtgg gcttacatgg cgatagctag actgggcggt tttatggaca gcaagcgaac
7021 cggaattgcc agctggggcg ccctctggta aggttgggaa gccctgcaaa gtaaactgga
7081 tggctttctc gccgccaagg atctgatggc gcaggggatc aagctctgat caagagacag
7141 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
7201 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
7261 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
7321 gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg
7381 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
7441 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
7501 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
7561 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
7621 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
7681 aggcgagcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
7741 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
7801 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
7861 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
7921 ccttctatcg ccttcttgac gagttcttct gaattttgtt aaaatttttg ttaaatcagc
7981 tcatttttta accaataggc cgaaatcggc aacatccctt ataaatcaaa agaatagacc
8041 gcgatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
8101 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
8161 cccaaatcaa gttttttgcg gtcgaggtgc cgtaaagctc taaatcggaa ccctaaaggg
8221 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
8281 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
8341 accacacccg cgcgcttaat gcgccgctac agggcgcgtc cattcgccat tcaggatcga
8401 attaattctt aattaacatc atcaataata tacctt
//
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 ttttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgt 60
AAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCA
61 gggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaa 120
CCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTT
Encap other(162,310)>>>
|
121 cacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgccggtgtacacagg 180
GTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCC
181 aagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagt 240
TTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCA
241 aagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttg 300
TTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAAC
301 tgttactcatagcgcgtaatacggcagacctcagcgctagattattgaagcatttatcag 360
ACAATGAGTATCGCGCATTATGCCGTCTGGAGTCGCGATCTAATAACTTCGTAAATAGTC
amp prom(365,393)<<<
|
361 ggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggg 420
CCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCC
HincII
|
HpaI
|
421 gttccgcgcacatttccccgaaaagtgccacctgacgttaactataacggtcctaaggta 480
CAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAATTGATATTGCCAGGATTCCAT
481 gcgaaaatgtagtcttatgcaatactcttgtagtcttgcaacatggtaacgatgagttag 540
CGCTTTTACATCAGAATACGTTATGAGAACATCAGAACGTTGTACCATTGCTACTCAATC
541 caacatgccttacaaggagagaaaaagcaccgtgcatgccgattggtggaagtaaggtgg 600
GTTGTACGGAATGTTCCTCTCTTTTTCGTGGCACGTACGGCTAACCACCTTCATTCCACC
601 tacgatcgtgccttattaggaaggcaacagacgggtctgacatggattggacgaaccact 660
ATGCTAGCACGGAATAATCCTTCCGTTGTCTGCCCAGACTGTACCTAACCTGCTTGGTGA
HIV-1_5_LTR other(715,895)>>>
|
661 gaattgccgcattgcagagatattgtatttaagtgcctagctcgatacataaacgggtct 720
CTTAACGGCGTAACGTCTCTATAACATAAATTCACGGATCGAGCTATGTATTTGCCCAGA
721 ctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgctt 780
GAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAA
781 aagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgac 840
TTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTG
841 tctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggc 900
AGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCACCG
901 gcccgaacagggacttgaaagcgaaagggaaaccagaggagctctctcgacgcaggactc 960
CGGGCTTGTCCCTGAACTTTCGCTTTCCCTTTGGTCTCCTCGAGAGAGCTGCGTCCTGAG
HIV-1_psi_pack other(1006,1050)>>>
|
961 ggcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaa 1020
CCGAACGACTTCGCGCGTGCCGTTCTCCGCTCCCCGCCGCTGACCACTCATGCGGTTTTT
1021 ttttgactagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcggg 1080
AAAACTGATCGCCTCCGATCTTCCTCTCTCTACCCACGCTCTCGCAGTCATAATTCGCCC
NruI
|
1081 ggagaattagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatata 1140
CCTCTTAATCTAGCGCTACCCTTTTTTAAGCCAATTCCGGTCCCCCTTTCTTTTTTATAT
1141 aattaaaacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcc 1200
TTAATTTTGTATATCATACCCGTTCGTCCCTCGATCTTGCTAAGCGTCAATTAGGACCGG
1201 tgttagaaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcaga 1260
ACAATCTTTGTAGTCTTCCGACATCTGTTTATGACCCTGTCGATGTTGGTAGGGAAGTCT
1261 caggatcagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatc 1320
GTCCTAGTCTTCTTGAATCTAGTAATATATTATGTCATCGTTGGGAGATAACACACGTAG
1321 aaaggatagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaaca 1380
TTTCCTATCTCTATTTTCTGTGGTTCCTTCGAAATCTGTTCTATCTCCTTCTCGTTTTGT
1381 aaagtaagaccaccgcacagcaagcccgctgatcttcagacctggaggaggagatatgag 1440
TTTCATTCTGGTGGCGTGTCGTTCGGGCGACTAGAAGTCTGGACCTCCTCCTCTATACTC
1441 ggacattggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagta 1500
CCTGTAACCTCTTCACTTAATATATTTATATTTCATCATTTTTAACTTGGTAATCCTCAT
RRE reg(1557,1790)>>>
|
1501 gcacccaccaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaatagga 1560
CGTGGGTGGTTCCGTTTCTCTTCTCACCACGTCTCTCTTTTTTCTCGTCACCCTTATCCT
ORF_1 rf(1)(1600,2301)>>>
|
1561 gctttgttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacg 1620
CGAAACAAGGAACCCAAGAACCCTCGTCGTCCTTCGTGATACCCGCGTCGCAGTTACTGC
1621 ctgacggtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctg 1680
GACTGCCATGTCCGGTCTGTTAATAACAGACCATATCACGTCGTCGTCTTGTTAAACGAC
1681 agggctattgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctc 1740
TCCCGATAACTCCGCGTTGTCGTAGACAACGTTGAGTGTCAGACCCCGTAGTTCGTCGAG
1741 caggcaagaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttgg 1800
GTCCGTTCTTAGGACCGACACCTTTCTATGGATTTCCTAGTTGTCGAGGACCCCTAAACC
1801 ggttgctctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaat 1860
CCAACGAGACCTTTTGAGTAAACGTGGTGACGACACGGAACCTTACGATCAACCTCATTA
1861 aaatctctggaacagatttggaatcacacgacctggatggagtgggacagagaaattaac 1920
TTTAGAGACCTTGTCTAAACCTTAGTGTGCTGGACCTACCTCACCCTGTCTCTTTAATTG
1921 aattacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaat 1980
TTAATGTGTTCGAATTATGTGAGGAATTAACTTCTTAGCGTTTTGGTCGTTCTTTTCTTA
1981 gaacaagaattattggaattagataaatgggcaagtttgtggaattggtttaacataaca 2040
CTTGTTCTTAATAACCTTAATCTATTTACCCGTTCAAACACCTTAACCAAATTGTATTGT
2041 aattggctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaaga 2100
TTAACCGACACCATATATTTTAATAAGTATTACTATCATCCTCCGAACCATCCAAATTCT
2101 atagtttttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcg 2160
TATCAAAAACGACATGAAAGATATCACTTATCTCAATCCGTCCCTATAAGTGGTAATAGC
2161 tttcagacccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaa 2220
AAAGTCTGGGTGGAGGGTTGGGGCTCCCCTGGGCTGTCCGGGCTTCCTTATCTTCTTCTT
2221 ggtggagagagagacagagacagatccattcgattagtgaacggatctcgacggtatcga 2280
CCACCTCTCTCTCTGTCTCTGTCTAGGTAAGCTAATCACTTGCCTAGAGCTGCCATAGCT
2281 aagcttgggattcgaatttaaaagaaaaggggggattggggggtacagtgcaggggaaag 2340
TTCGAACCCTAAGCTTAAATTTTCTTTTCCCCCCTAACCCCCCATGTCACGTCCCCTTTC
2341 aatagtagacataatagcaacagacatacaaactaaagaactacaaaaacaaattacaaa 2400
TTATCATCTGTATTATCGTTGTCTGTATGTTTGATTTCTTGATGTTTTTGTTTAATGTTT
2401 aattcaaaattttcgggtttttcgaacctagggttccgcgttacataacttacggtaaat 2460
TTAAGTTTTAAAAGCCCAAAAAGCTTGGATCCCAAGGCGCAATGTATTGAATGCCATTTA
2461 ggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgtt 2520
CCGGGCGGACCGACTGGCGGGTTGCTGGGGGCGGGTAACTGCAGTTATTACTGCATACAA
2521 cccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaa 2580
GGGTATCATTGCGGTTATCCCTGAAAGGTAACTGCAGTTACCCACCTCATAAATGCCATT
NdeI
|
2581 actgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtc 2640
TGACGGGTGAACCGTCATGTAGTTCACATAGTATACGGTTCATGCGGGGGATAACTGCAG
2641 aatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcct 2700
TTACTGCCATTTACCGGGCGGACCGTAATACGGGTCATGTACTGGAATACCCTGAAAGGA
2701 acttggcagtacatctacgtttagtcatcgctattaccatggtgatgcggttttggcagt 2760
TGAACCGTCATGTAGATGCAAATCAGTAGCGATAATGGTACCACTACGCCAAAACCGTCA
2761 acatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattg 2820
TGTAGTTACCCGCACCTATCGCCAAACTGAGTGCCCCTAAAGGTTCAGAGGTGGGGTAAC
2821 acgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaaca 2880
TGCAGTTACCCTCAAACAAAACCGTGGTTTTAGTTGCCCTGAAAGGTTTTACAGCATTGT
CMV prom(2885,2966)>>>
|
2881 actccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagca 2940
TGAGGCGGGGTAACTGCGTTTACCCGCCATCCGCACATGCCACCCTCCAGATATATTCGT
2941 gagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctcc 3000
CTCGAGCAAATCACTTGGCAGTCTAGCGGACCTCTGCGGTAGGTGCGACAAAACTGGAGG
tetO reg(3022,3061)>>>
|
3001 atagaagaaccgagtttaaactccctatcagtgatagagatctccctatcagtgatagag 3060
TATCTTCTTGGCTCAAATTTGAGGGATAGTCACTATCTCTAGAGGGATAGTCACTATCTC
SmaI
|
XmaI ClaI KpnI
| | | |
3061 agctagccccgggatcgatcaattgagtacttacgtaggtaccaatcagctcgctcacca 3120
TCGATCGGGGCCCTAGCTAGTTAACTCATGAATGCATCCATGGTTAGTCGAGCGAGTGGT
3121 tataacttcgtataaagtatcctatacgaagttatatcaaaataggaagaccaatgcttc 3180
ATATTGAAGCATATTTCATAGGATATGCTTCAATATAGTTTTATCCTTCTGGTTACGAAG
loxP other(3218,3251)<<<
|
3181 accatcgacccgaattgccaagcatcaccatcgacccataacttcgtataatgtatgcta 3240
TGGTAGCTGGGCTTAACGGTTCGTAGTGGTAGCTGGGTATTGAAGCATATTACATACGAT
EcoRV
|
3241 tacgaagttatctcgagtcagatatcgctagccaattgataacttcgtataggatacttt 3300
ATGCTTCAATAGAGCTCAGTCTATAGCGATCGGTTAACTATTGAAGCATATCCTATGAAA
3301 atacgaagttatcattgggattcttcctattttgatccaagcatcaccatcgaccctcta 3360
TATGCTTCAATAGTAACCCTAAGAAGGATAAAACTAGGTTCGTAGTGGTAGCTGGGAGAT
loxP other(3383,3416)>>>
|
3361 gtccagatctcaccatcgacccataacttcgtatagcatacattatacgaagttatatgg 3420
CAGGTCTAGAGTGGTAGCTGGGTATTGAAGCATATCGTATGTAATATGCTTCAATATACC
XbaI PstI
| |
3421 tgagcaagggcgattctagactgcagctcgagtacccatacgacgtcccagactacgctt 3480
ACTCGTTCCCGCTAAGATCTGACGTCGAGCTCATGGGTATGCTGCAGGGTCTGATGCGAA
SV40 prom(3497,3765)>>>
|
3481 gagtttaaacacgcgtggtgtggaaagtccccaggctccccagcaggcagaagtatgcaa 3540
CTCAAATTTGTGCGCACCACACCTTTCAGGGGTCCGAGGGGTCGTCCGTCTTCATACGTT
3541 agcatgcatctcaattagtcagcaaccaggtgtggaaagtccccaggctccccagcaggc 3600
TCGTACGTAGAGTTAATCAGTCGTTGGTCCACACCTTTCAGGGGTCCGAGGGGTCGTCCG
3601 agaagtatgcaaagcatgcatctcaattagtcagcaaccatagtcccgcccctaactccg 3660
TCTTCATACGTTTCGTACGTAGAGTTAATCAGTCGTTGGTATCAGGGCGGGGATTGAGGC
SV40 origin(3664,3741)>>>
|
3661 cccatcccgcccctaactccgcccagttccgcccattctccgccccatggctgactaatt 3720
GGGTAGGGCGGGGATTGAGGCGGGTCAAGGCGGGTAAGAGGCGGGGTACCGACTGATTAA
SfiI
|
ORF_2 rf(2)(3731,4399)>>>
| |
3721 ttttttatttatgcagaggccgaggccgcctcggcctctgagctattccagaagtagtga 3780
AAAAAATAAATACGTCTCCGGCTCCGGCGGAGCCGGAGACTCGATAAGGTCTTCATCACT
puro marker(3800,4399)>>>
|
3781 ggaggcttttttggaggccatgaccgagtacaagcccacggtgcgcctcgccacccgcga 3840
CCTCCGAAAAAACCTCCGGTACTGGCTCATGTTCGGGTGCCACGCGGAGCGGTGGGCGCT
3841 cgacgtccctcgggccgtacgcaccctcgccgccgcgttcgccgactaccccgccacgcg 3900
GCTGCAGGGAGCCCGGCATGCGTGGGAGCGGCGGCGCAAGCGGCTGATGGGGCGGTGCGC
3901 ccacaccgtggacccggaccgccacatcgagcgggtcaccgagctgcaagaactcttcct 3960
GGTGTGGCACCTGGGCCTGGCGGTGTAGCTCGCCCAGTGGCTCGACGTTCTTGAGAAGGA
3961 cacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtggc 4020
GTGCGCGCAGCCCGAGCTGTAGCCGTTCCACACCCAGCGCCTGCTGCCGCGGCGCCACCG
4021 ggtctggaccacgccggagagcgtcgaagcgggggcggtgttcgccgagatcggcccgcg 4080
CCAGACCTGGTGCGGCCTCTCGCAGCTTCGCCCCCGCCACAAGCGGCTCTAGCCGGGCGC
4081 catggccgagttgagcggttcccggctggccgcgcagcaacagatggaagggctcctggc 4140
GTACCGGCTCAACTCGCCAAGGGCCGACCGGCGCGTCGTTGTCTACCTTCCCGAGGACCG
4141 gccgcaccggcccaaggagcccgcgtggttcctggccaccgtcggcgtctcgcccgacca 4200
CGGCGTGGCCGGGTTCCTCGGGCGCACCAAGGACCGGTGGCAGCCGCAGAGCGGGCTGGT
4201 ccagggcaagggtctgggcagcgccgtcgtgctccccggagtggaggcggccgagcgcgc 4260
GGTCCCGTTCCCAGACCCGTCGCGGCAGCACGAGGGGCCTCACCTCCGCCGGCTCGCGCG
4261 cggggtgcccgccttcctggagacctccgcgccccgcaacctccccttctacgagcggct 4320
GCCCCACGGGCGGAAGGACCTCTGGAGGCGCGGGGCGTTGGAGGGGAAGATGCTCGCCGA
4321 cggcttcaccgtcaccgccgacgtcgaggtgcccgaaggaccgcgcacctggtgcatgac 4380
GCCGAAGTGGCAGTGGCGGCTGCAGCTCCACGGGCTTCCTGGCGCGTGGACCACGTACTG
4381 ccgcaagcccggtgcctgaacgcgttccggaaatcaacctctggattacaaaatttgtga 4440
GGCGTTCGGGCCACGGACTTGCGCAAGGCCTTTAGTTGGAGACCTAATGTTTTAAACACT
4441 aagattgactggtattcttaactatgttgctccttttacgctatgtggatacgctgcttt 4500
TTCTAACTGACCATAAGAATTGATACAACGAGGAAAATGCGATACACCTATGCGACGAAA
4501 aatgcctttgtatcatgctattgcttcccgtatggctttcattttctcctccttgtataa 4560
TTACGGAAACATAGTACGATAACGAAGGGCATACCGAAAGTAAAAGAGGAGGAACATATT
4561 atcctggttgctgtctctttatgaggagttgtggcccgttgtcaggcaacgtggcgtggt 4620
TAGGACCAACGACAGAGAAATACTCCTCAACACCGGGCAACAGTCCGTTGCACCGCACCA
4621 gtgcactgtgtttgctgacgcaacccccactggttggggcattgccaccacctgtcagct 4680
CACGTGACACAAACGACTGCGTTGGGGGTGACCAACCCCGTAACGGTGGTGGACAGTCGA
4681 cctttccgggactttcgctttccccctccctattgccacggcggaactcatcgccgcctg 4740
GGAAAGGCCCTGAAAGCGAAAGGGGGAGGGATAACGGTGCCGCCTTGAGTAGCGGCGGAC
4741 ccttgcccgctgctggacaggggctcggctgttgggcactgacaattccgtggtgttgtc 4800
GGAACGGGCGACGACCTGTCCCCGAGCCGACAACCCGTGACTGTTAAGGCACCACAACAG
4801 ggggaagctgacgtcctttccatggctgctcgcctgtgttgccacctggattctgcgcgg 4860
CCCCTTCGACTGCAGGAAAGGTACCGACGAGCGGACACAACGGTGGACCTAAGACGCGCC
4861 gacgtccttctgctacgtcccttcggccctcaatccagcggaccttccttcccgcggcct 4920
CTGCAGGAAGACGATGCAGGGAAGCCGGGAGTTAGGTCGCCTGGAAGGAAGGGCGCCGGA
4921 gctgccggctctgcggcctcttccgcgtctcgccttcgccctcagacgagtcggatctcc 4980
CGACGGCCGAGACGCCGGAGAAGGCGCAGAGCGGAAGCGGGAGTCTGCTCAGCCTAGAGG
delta_U3 other(5009,5061)>>>
|
4981 ctttgggccgcctccccgcctgtccggatggaagggctaattcactcccaacgaatacaa 5040
GAAACCCGGCGGAGGGGCGGACAGGCCTACCTTCCCGATTAAGTGAGGGTTGCTTATGTT
HIV-1_5_LTR other(5062,5242)>>>
|
5041 gatctgctttttgcttgtactgggtctctctggttagaccagatctgagcctgggagctc 5100
CTAGACGAAAAACGAACATGACCCAGAGAGACCAATCTGGTCTAGACTCGGACCCTCGAG
5101 tctggctaactagggaacccactgcttaagcctcaataaagcttgccttgagtgcttcaa 5160
AGACCGATTGATCCCTTGGGTGACGAATTCGGAGTTATTTCGAACGGAACTCACGAAGTT
5161 gtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttag 5220
CATCACACACGGGCAGACAACACACTGAGACCATTGATCTCTAGGGAGTCTGGGAAAATC
5221 tcagtgtggaaaatctctagcagtagtagttcatgtcatcttattattcagtatttataa 5280
AGTCACACCTTTTAGAGATCGTCATCATCAAGTACAGTAGAATAATAAGTCATAAATATT
5281 cttgcaaagaaatgaatatcagagagtgagaggaacttgtttattgcagcttataatggt 5340
GAACGTTTCTTTACTTATAGTCTCTCACTCTCCTTGAACAAATAACGTCGAATATTACCA
5341 tacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattct 5400
ATGTTTATTTCGTTATCGTAGTGTTTAAAGTGTTTATTTCGTAAAAAAAGTGACGTAAGA
5401 agttgtggtttgtccaaactcatcaatgtatcttatcatgtctggcatctatgtcgggtg 5460
TCAACACCAAACAGGTTTGAGTAGTTACATAGAATAGTACAGACCGTAGATACAGCCCAC
5461 cggagaaagaggtaatgaaatggcattatgggtattatgggtctgcattaatgaatcggc 5520
GCCTCTTTCTCCATTACTTTACCGTAATACCCATAATACCCAGACGTAATTACTTAGCCG
5521 caacgatcccggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgc 5580
GTTGCTAGGGCCACACTTTATGGCGTGTCTACGCATTCCTCTTTTATGGCGTAGTCCGCG
5581 tcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggta 5640
AGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGAGCCAGCAAGCCGACGCCGCTCGCCAT
5641 tcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaag 5700
AGTCGAGTGAGTTTCCGCCATTATGCCAATAGGTGTCTTAGTCCCCTATTGCGTCCTTTC
pBR322 origin(5748,6367)<<<
|
5701 aacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcg 5760
TTGTACACTCGTTTTCCGGTCGTTTTCCGGTCCTTGGCATTTTTCCGGCGCAACGACCGC
5761 tttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagagg 5820
AAAAAGGTATCCGAGGCGGGGGGACTGCTCGTAGTGTTTTTAGCTGCGAGTTCAGTCTCC
5821 tggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtg 5880
ACCGCTTTGGGCTGTCCTGATATTTCTATGGTCCGCAAAGGGGGACCTTCGAGGGAGCAC
5881 cgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcggga 5940
GCGAGAGGACAAGGCTGGGACGGCGAATGGCCTATGGACAGGCGGAAAGAGGGAAGCCCT
5941 agcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgc 6000
TCGCACCGCGAAAGAGTATCGAGTGCGACATCCATAGAGTCAAGCCACATCCAGCAAGCG
6001 tccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggt 6060
AGGTTCGACCCGACACACGTGCTTGGGGGGCAAGTCGGGCTGGCGACGCGGAATAGGCCA
6061 aactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccact 6120
TTGATAGCAGAACTCAGGTTGGGCCATTCTGTGCTGAATAGCGGTGACCGTCGTCGGTGA
6121 ggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtgg 6180
CCATTGTCCTAATCGTCTCGCTCCATACATCCGCCACGATGTCTCAAGAACTTCACCACC
6181 cctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagtt 6240
GGATTGATGCCGATGTGATCTTCCTGTCATAAACCATAGACGCGAGACGACTTCGGTCAA
6241 accttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggt 6300
TGGAAGCCTTTTTCTCAACCATCGAGAACTAGGCCGTTTGTTTGGTGGCGACCATCGCCA
6301 ggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcct 6360
CCAAAAAAACAAACGTTCGTCGTCTAATGCGCGTCTTTTTTTCCTAGAGTTCTTCTAGGA
6361 ttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttg 6420
AACTAGAAAAGATGCCCCAGACTGCGAGTCACCTTGCTTTTGAGTGCAATTCCCTAAAAC
6421 gtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagtttt 6480
CAGTACTCTAATAGTTTTTCCTAGAAGTGGATCTAGGAAAATTTAATTTTTACTTCAAAA
6481 aaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagt 6540
TTTAGTTAGATTTCATATATACTCATTTGAACCAGACTGTCAATGGTTACGAATTAGTCA
6541 gaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtc 6600
CTCCGTGGATAGAGTCGCTAGACAGATAAAGCAAGTAGGTATCAACGGACTGAGGGGCAG
6601 gtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccg 6660
CACATCTATTGATGCTATGCCCTCCCGAATGGTAGACCGGGGTCACGACGTTACTATGGC
6661 cgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggcc 6720
GCTCTGGGTGCGAGTGGCCGAGGTCTAAATAGTCGTTATTTGGTCGGTCGGCCTTCCCGG
6721 gagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgg 6780
CTCGCGTCTTCACCAGGACGTTGAAATAGGCGGAGGTAGGTCAGATAATTAACAACGGCC
6781 gaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgaaaaaggatct 6840
CTTCGATCTCATTCATCAAGCGGTCAATTATCAAACGCGTTGCAACAACTTTTTCCTAGA
6841 tcacctagatccttttcacgtagaaagccagtccgcagaaacggtgctgaccccggatga 6900
AGTGGATCTAGGAAAAGTGCATCTTTCGGTCAGGCGTCTTTGCCACGACTGGGGCCTACT
6901 atgtcagctactgggctatctggacaagggaaaacgcaagcgcaaagagaaagcaggtag 6960
TACAGTCGATGACCCGATAGACCTGTTCCCTTTTGCGTTCGCGTTTCTCTTTCGTCCATC
6961 cttgcagtgggcttacatggcgatagctagactgggcggttttatggacagcaagcgaac 7020
GAACGTCACCCGAATGTACCGCTATCGATCTGACCCGCCAAAATACCTGTCGTTCGCTTG
NEOKAN prom(7020,7069)>>>
|
7021 cggaattgccagctggggcgccctctggtaaggttgggaagccctgcaaagtaaactgga 7080
GCCTTAACGGTCGACCCCGCGGGAGACCATTCCAACCCTTCGGGACGTTTCATTTGACCT
BclI
|
7081 tggctttctcgccgccaaggatctgatggcgcaggggatcaagctctgatcaagagacag 7140
ACCGAAAGAGCGGCGGTTCCTAGACTACCGCGTCCCCTAGTTCGAGACTAGTTCTCTGTC
NTP_II marker(7161,7949)>>>
|
ORF_3 rf(3)(7158,7952)>>>
| |
7141 gatgaggatcgtttcgcatgattgaacaagatggattgcacgcaggttctccggccgctt 7200
CTACTCCTAGCAAAGCGTACTAACTTGTTCTACCTAACGTGCGTCCAAGAGGCCGGCGAA
7201 gggtggagaggctattcggctatgactgggcacaacagacaatcggctgctctgatgccg 7260
CCCACCTCTCCGATAAGCCGATACTGACCCGTGTTGTCTGTTAGCCGACGAGACTACGGC
7261 ccgtgttccggctgtcagcgcaggggcgcccggttctttttgtcaagaccgacctgtccg 7320
GGCACAAGGCCGACAGTCGCGTCCCCGCGGGCCAAGAAAAACAGTTCTGGCTGGACAGGC
7321 gtgccctgaatgaactgcaagacgaggcagcgcggctatcgtggctggccacgacgggcg 7380
CACGGGACTTACTTGACGTTCTGCTCCGTCGCGCCGATAGCACCGACCGGTGCTGCCCGC
7381 ttccttgcgcagctgtgctcgacgttgtcactgaagcgggaagggactggctgctattgg 7440
AAGGAACGCGTCGACACGAGCTGCAACAGTGACTTCGCCCTTCCCTGACCGACGATAACC
7441 gcgaagtgccggggcaggatctcctgtcatctcaccttgctcctgccgagaaagtatcca 7500
CGCTTCACGGCCCCGTCCTAGAGGACAGTAGAGTGGAACGAGGACGGCTCTTTCATAGGT
7501 tcatggctgatgcaatgcggcggctgcatacgcttgatccggctacctgcccattcgacc 7560
AGTACCGACTACGTTACGCCGCCGACGTATGCGAACTAGGCCGATGGACGGGTAAGCTGG
7561 accaagcgaaacatcgcatcgagcgagcacgtactcggatggaagccggtcttgtcgatc 7620
TGGTTCGCTTTGTAGCGTAGCTCGCTCGTGCATGAGCCTACCTTCGGCCAGAACAGCTAG
7621 aggatgatctggacgaagagcatcaggggctcgcgccagccgaactgttcgccaggctca 7680
TCCTACTAGACCTGCTTCTCGTAGTCCCCGAGCGCGGTCGGCTTGACAAGCGGTCCGAGT
7681 aggcgagcatgcccgacggcgaggatctcgtcgtgacccatggcgatgcctgcttgccga 7740
TCCGCTCGTACGGGCTGCCGCTCCTAGAGCAGCACTGGGTACCGCTACGGACGAACGGCT
7741 atatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtgtgg 7800
TATAGTACCACCTTTTACCGGCGAAAAGACCTAAGTAGCTGACACCGGCCGACCCACACC
7801 cggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcg 7860
GCCTGGCGATAGTCCTGTATCGCAACCGATGGGCACTATAACGACTTCTCGAACCGCCGC
7861 aatgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcg 7920
TTACCCGACTGGCGAAGGAGCACGAAATGCCATAGCGGCGAGGGCTAAGCGTCGCGTAGC
7921 ccttctatcgccttcttgacgagttcttctgaattttgttaaaatttttgttaaatcagc 7980
GGAAGATAGCGGAAGAACTGCTCAAGAAGACTTAAAACAATTTTAAAAACAATTTAGTCG
7981 tcattttttaaccaataggccgaaatcggcaacatcccttataaatcaaaagaatagacc 8040
AGTAAAAAATTGGTTATCCGGCTTTAGCCGTTGTAGGGAATATTTAGTTTTCTTATCTGG
f1 origin(8057,8362)<<<
|
8041 gcgatagggttgagtgttgttccagtttggaacaagagtccactattaaagaacgtggac 8100
CGCTATCCCAACTCACAACAAGGTCAAACCTTGTTCTCAGGTGATAATTTCTTGCACCTG
8101 tccaacgtcaaagggcgaaaaaccgtctatcagggcgatggcccactacgtgaaccatca 8160
AGGTTGCAGTTTCCCGCTTTTTGGCAGATAGTCCCGCTACCGGGTGATGCACTTGGTAGT
8161 cccaaatcaagttttttgcggtcgaggtgccgtaaagctctaaatcggaaccctaaaggg 8220
GGGTTTAGTTCAAAAAACGCCAGCTCCACGGCATTTCGAGATTTAGCCTTGGGATTTCCC
8221 agcccccgatttagagcttgacggggaaagccggcgaacgtggcgagaaaggaagggaag 8280
TCGGGGGCTAAATCTCGAACTGCCCCTTTCGGCCGCTTGCACCGCTCTTTCCTTCCCTTC
8281 aaagcgaaaggagcgggcgctagggcgctggcaagtgtagcggtcacgctgcgcgtaacc 8340
TTTCGCTTTCCTCGCCCGCGATCCCGCGACCGTTCACATCGCCAGTGCGACGCGCATTGG
8341 accacacccgcgcgcttaatgcgccgctacagggcgcgtccattcgccattcaggatcga 8400
TGGTGTGGGCGCGCGAATTACGCGGCGATGTCCCGCGCAGGTAAGCGGTAAGTCCTAGCT
8401 attaattcttaattaacatcatcaataatatacctt 8436
TAATTAAGAATTAATTGTAGTAGTTATTATATGGAA
LOCUS dna 8436 bp
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
misc_binding 457..462
/dbxref="REBASE:HpaI"
misc_binding 457..462
/dbxref="REBASE:HincII"
Other Gene 715..895
/gene="HIV-1_5_LTR other"
Other Gene 1006..1050
/gene="HIV-1_psi_pack other"
misc_binding 1092..1097
/dbxref="REBASE:NruI"
Regulatory_Seq 1557..1790
/gene="RRE reg"
ORF 1600..2301
/sequence="ORF_1 rf(1)"
misc_binding 2611..2616
/dbxref="REBASE:NdeI"
Promoter 2885..2966
/gene="CMV prom"
Regulatory_Seq 3022..3061
/gene="tetO reg"
misc_binding 3068..3073
/dbxref="REBASE:SmaI"
misc_binding 3068..3073
/dbxref="REBASE:XmaI"
misc_binding 3074..3079
/dbxref="REBASE:ClaI"
misc_binding 3098..3103
/dbxref="REBASE:KpnI"
Other Gene 3218..3251
/gene="loxP other"
misc_binding 3261..3266
/dbxref="REBASE:EcoRV"
Other Gene 3383..3416
/gene="loxP other"
misc_binding 3435..3440
/dbxref="REBASE:XbaI"
misc_binding 3441..3446
/dbxref="REBASE:PstI"
Promoter 3497..3765
/gene="SV40 prom"
Rep_Origin 3664..3741
/gene="SV40 origin"
ORF 3731..4399
/sequence="ORF_2 rf(2)"
misc_binding 3744..3756
/dbxref="REBASE:SfiI"
Marker 3800..4399
/gene="puro marker"
Other Gene 5009..5061
/gene="delta_U3 other"
Other Gene 5062..5242
/gene="HIV-1_5_LTR other"
Rep_Origin 5748..6367
/gene="pBR322 origin"
Promoter 7020..7069
/gene="NEOKAN prom"
misc_binding 7127..7132
/dbxref="REBASE:BclI"
ORF 7158..7952
/sequence="ORF_3 rf(3)"
Marker 7161..7949
/gene="NTP_II marker"
Rep_Origin 8057..8362
/gene="f1 origin"
BASE COUNT 2149 a 2071 c 2238 g 1978 t 0 others