pLenti-CMV-DIO-Blank-SV40-GFP
| Vector Name | pLenti-CMV-DIO-Blank-SV40-GFP |
| VectorType | Lentiviral Vector |
| Antibiotic Information | Kanamycin |
| Sequencing Primers | CMV sequencing primer 5`---CGC AAA TGG GCG GTA GGC GTG---3` |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta
481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag
1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga
2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag
2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa
2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat
2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag
3061 agctagcccc gggatcgatc aattgagtac ttacgtaggt accaatcagc tcgctcacca
3121 tataacttcg tataaagtat cctatacgaa gttatatcaa aataggaaga ccaatgcttc
3181 accatcgacc cgaattgcca agcatcacca tcgacccata acttcgtata atgtatgcta
3241 tacgaagtta tctcgagtca gatatcgcta gccaattgat aacttcgtat aggatacttt
3301 atacgaagtt atcattggga ttcttcctat tttgatccaa gcatcaccat cgaccctcta
3361 gtccagatct caccatcgac ccataacttc gtatagcata cattatacga agttatatgg
3421 tgagcaaggg cgattctaga ctgcagctcg agtacccata cgacgtccca gactacgctt
3481 gagtttaaac acgcgtggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
3541 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
3601 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
3661 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
3721 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
3781 ggaggctttt ttggaggccg ccaccatgga gagcgacgag agcggcctgc ccgccatgga
3841 gatcgagtgc cgcatcaccg gcaccctgaa cggcgtggag ttcgagctgg tgggcggcgg
3901 agagggcacc cccaagcagg gccgcatgac caacaagatg aagagcacca aaggcgccct
3961 gaccttcagc ccctacctgc tgagccacgt gatgggctac ggcttctacc acttcggcac
4021 ctaccccagc ggctacgaga accccttcct gcacgccatc aacaacggcg gctacaccaa
4081 cacccgcatc gagaagtacg aggacggcgg cgtgctgcac gtgagcttca gctaccgcta
4141 cgaggccggc cgcgtgatcg gcgacttcaa ggtggtgggc accggcttcc ccgaggacag
4201 cgtgatcttc accgacaaga tcatccgcag caacgccacc gtggagcacc tgcaccccat
4261 gggcgataac gtgctggtgg gcagcttcgc ccgcaccttc agcctgcgcg acggcggcta
4321 ctacagcttc gtggtggaca gccacatgca cttcaagagc gccatccacc ccagcatcct
4381 gcagaacggg ggccccatgt tcgccttccg ccgcgtggag gagctgcaca gcaacaccga
4441 gctgggcatc gtggagtacc agcacgcctt caagaccccc atcgccttcg ccagatcccg
4501 cgctcagtcg tccaattctg ccgtggacgg caccgccgga cccggctcca ccggatctcg
4561 ctgaacgcgt tccggaaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
4621 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
4681 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
4741 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
4801 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
4861 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
4921 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc
4981 ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
5041 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
5101 gcctcttccg cgtctcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
5161 ccgcctgtcc ggatggaagg gctaattcac tcccaacgaa tacaagatct gctttttgct
5221 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
5281 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
5341 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
5401 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
5461 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat
5521 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
5581 aaactcatca atgtatctta tcatgtctgg catctatgtc gggtgcggag aaagaggtaa
5641 tgaaatggca ttatgggtat tatgggtctg cattaatgaa tcggccaacg atcccggtgt
5701 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg
5761 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
5821 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
5881 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
5941 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
6001 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
6061 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
6121 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
6181 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
6241 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
6301 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
6361 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
6421 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
6481 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
6541 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
6601 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
6661 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
6721 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
6781 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
6841 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
6901 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
6961 agttcgccag ttaatagttt gcgcaacgtt gttgaaaaag gatcttcacc tagatccttt
7021 tcacgtagaa agccagtccg cagaaacggt gctgaccccg gatgaatgtc agctactggg
7081 ctatctggac aagggaaaac gcaagcgcaa agagaaagca ggtagcttgc agtgggctta
7141 catggcgata gctagactgg gcggttttat ggacagcaag cgaaccggaa ttgccagctg
7201 gggcgccctc tggtaaggtt gggaagccct gcaaagtaaa ctggatggct ttctcgccgc
7261 caaggatctg atggcgcagg ggatcaagct ctgatcaaga gacaggatga ggatcgtttc
7321 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
7381 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
7441 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
7501 tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
7561 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
7621 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
7681 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
7741 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
7801 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg
7861 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
7921 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
7981 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
8041 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
8101 ttgacgagtt cttctgaatt ttgttaaaat ttttgttaaa tcagctcatt ttttaaccaa
8161 taggccgaaa tcggcaacat cccttataaa tcaaaagaat agaccgcgat agggttgagt
8221 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
8281 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccaa atcaagtttt
8341 ttgcggtcga ggtgccgtaa agctctaaat cggaacccta aagggagccc ccgatttaga
8401 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
8461 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgcgcgc
8521 ttaatgcgcc gctacagggc gcgtccattc gccattcagg atcgaattaa ttcttaatta
8581 acatcatcaa taatatacct t
LOCUS dna 8601 bp
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
misc_binding 457..462
/dbxref="REBASE:HpaI"
misc_binding 457..462
/dbxref="REBASE:HincII"
Other Gene 715..895
/gene="HIV-1_5_LTR other"
Other Gene 1006..1050
/gene="HIV-1_psi_pack other"
misc_binding 1092..1097
/dbxref="REBASE:NruI"
Regulatory_Seq 1557..1790
/gene="RRE reg"
ORF 1600..2301
/sequence="ORF_1 rf(1)"
misc_binding 2611..2616
/dbxref="REBASE:NdeI"
Promoter 2885..2966
/gene="CMV prom"
Regulatory_Seq 3022..3061
/gene="tetO reg"
misc_binding 3068..3073
/dbxref="REBASE:SmaI"
misc_binding 3068..3073
/dbxref="REBASE:XmaI"
misc_binding 3074..3079
/dbxref="REBASE:ClaI"
misc_binding 3098..3103
/dbxref="REBASE:KpnI"
Other Gene 3218..3251
/gene="loxP other"
misc_binding 3261..3266
/dbxref="REBASE:EcoRV"
Other Gene 3383..3416
/gene="loxP other"
misc_binding 3435..3440
/dbxref="REBASE:XbaI"
Promoter 3497..3765
/gene="SV40 prom"
Rep_Origin 3664..3741
/gene="SV40 origin"
ORF 3731..4564
/sequence="ORF_2 rf(2)"
misc_binding 3744..3756
/dbxref="REBASE:SfiI"
misc_binding 4390..4395
/dbxref="REBASE:ApaI"
Other Gene 5174..5226
/gene="delta_U3 other"
Other Gene 5227..5407
/gene="HIV-1_5_LTR other"
Rep_Origin 5913..6532
/gene="pBR322 origin"
Promoter 7185..7234
/gene="NEOKAN prom"
misc_binding 7292..7297
/dbxref="REBASE:BclI"
ORF 7323..8117
/sequence="ORF_3 rf(3)"
Marker 7326..8114
/gene="NTP_II marker"
Rep_Origin 8222..8527
/gene="f1 origin"
BASE COUNT 2218 a 2114 c 2256 g 2013 t 0 others
ORIGIN
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta
481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag
1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga
2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag
2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa
2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat
2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag
3061 agctagcccc gggatcgatc aattgagtac ttacgtaggt accaatcagc tcgctcacca
3121 tataacttcg tataaagtat cctatacgaa gttatatcaa aataggaaga ccaatgcttc
3181 accatcgacc cgaattgcca agcatcacca tcgacccata acttcgtata atgtatgcta
3241 tacgaagtta tctcgagtca gatatcgcta gccaattgat aacttcgtat aggatacttt
3301 atacgaagtt atcattggga ttcttcctat tttgatccaa gcatcaccat cgaccctcta
3361 gtccagatct caccatcgac ccataacttc gtatagcata cattatacga agttatatgg
3421 tgagcaaggg cgattctaga ctgcagctcg agtacccata cgacgtccca gactacgctt
3481 gagtttaaac acgcgtggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
3541 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
3601 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
3661 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
3721 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
3781 ggaggctttt ttggaggccg ccaccatgga gagcgacgag agcggcctgc ccgccatgga
3841 gatcgagtgc cgcatcaccg gcaccctgaa cggcgtggag ttcgagctgg tgggcggcgg
3901 agagggcacc cccaagcagg gccgcatgac caacaagatg aagagcacca aaggcgccct
3961 gaccttcagc ccctacctgc tgagccacgt gatgggctac ggcttctacc acttcggcac
4021 ctaccccagc ggctacgaga accccttcct gcacgccatc aacaacggcg gctacaccaa
4081 cacccgcatc gagaagtacg aggacggcgg cgtgctgcac gtgagcttca gctaccgcta
4141 cgaggccggc cgcgtgatcg gcgacttcaa ggtggtgggc accggcttcc ccgaggacag
4201 cgtgatcttc accgacaaga tcatccgcag caacgccacc gtggagcacc tgcaccccat
4261 gggcgataac gtgctggtgg gcagcttcgc ccgcaccttc agcctgcgcg acggcggcta
4321 ctacagcttc gtggtggaca gccacatgca cttcaagagc gccatccacc ccagcatcct
4381 gcagaacggg ggccccatgt tcgccttccg ccgcgtggag gagctgcaca gcaacaccga
4441 gctgggcatc gtggagtacc agcacgcctt caagaccccc atcgccttcg ccagatcccg
4501 cgctcagtcg tccaattctg ccgtggacgg caccgccgga cccggctcca ccggatctcg
4561 ctgaacgcgt tccggaaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
4621 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
4681 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
4741 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
4801 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
4861 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
4921 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc
4981 ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
5041 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
5101 gcctcttccg cgtctcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
5161 ccgcctgtcc ggatggaagg gctaattcac tcccaacgaa tacaagatct gctttttgct
5221 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
5281 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
5341 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
5401 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
5461 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat
5521 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
5581 aaactcatca atgtatctta tcatgtctgg catctatgtc gggtgcggag aaagaggtaa
5641 tgaaatggca ttatgggtat tatgggtctg cattaatgaa tcggccaacg atcccggtgt
5701 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg
5761 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
5821 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
5881 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
5941 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
6001 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
6061 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
6121 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
6181 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
6241 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
6301 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
6361 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
6421 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
6481 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
6541 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
6601 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
6661 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
6721 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
6781 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
6841 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
6901 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
6961 agttcgccag ttaatagttt gcgcaacgtt gttgaaaaag gatcttcacc tagatccttt
7021 tcacgtagaa agccagtccg cagaaacggt gctgaccccg gatgaatgtc agctactggg
7081 ctatctggac aagggaaaac gcaagcgcaa agagaaagca ggtagcttgc agtgggctta
7141 catggcgata gctagactgg gcggttttat ggacagcaag cgaaccggaa ttgccagctg
7201 gggcgccctc tggtaaggtt gggaagccct gcaaagtaaa ctggatggct ttctcgccgc
7261 caaggatctg atggcgcagg ggatcaagct ctgatcaaga gacaggatga ggatcgtttc
7321 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
7381 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
7441 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
7501 tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
7561 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
7621 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
7681 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
7741 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
7801 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg
7861 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
7921 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
7981 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
8041 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
8101 ttgacgagtt cttctgaatt ttgttaaaat ttttgttaaa tcagctcatt ttttaaccaa
8161 taggccgaaa tcggcaacat cccttataaa tcaaaagaat agaccgcgat agggttgagt
8221 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
8281 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccaa atcaagtttt
8341 ttgcggtcga ggtgccgtaa agctctaaat cggaacccta aagggagccc ccgatttaga
8401 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
8461 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgcgcgc
8521 ttaatgcgcc gctacagggc gcgtccattc gccattcagg atcgaattaa ttcttaatta
8581 acatcatcaa taatatacct t
//
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 ttttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgt 60
AAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCA
61 gggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaa 120
CCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTT
Encap other(162,310)>>>
|
121 cacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgccggtgtacacagg 180
GTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCC
181 aagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagt 240
TTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCA
241 aagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttg 300
TTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAAC
301 tgttactcatagcgcgtaatacggcagacctcagcgctagattattgaagcatttatcag 360
ACAATGAGTATCGCGCATTATGCCGTCTGGAGTCGCGATCTAATAACTTCGTAAATAGTC
amp prom(365,393)<<<
|
361 ggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggg 420
CCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCC
HincII
|
HpaI
|
421 gttccgcgcacatttccccgaaaagtgccacctgacgttaactataacggtcctaaggta 480
CAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAATTGATATTGCCAGGATTCCAT
481 gcgaaaatgtagtcttatgcaatactcttgtagtcttgcaacatggtaacgatgagttag 540
CGCTTTTACATCAGAATACGTTATGAGAACATCAGAACGTTGTACCATTGCTACTCAATC
541 caacatgccttacaaggagagaaaaagcaccgtgcatgccgattggtggaagtaaggtgg 600
GTTGTACGGAATGTTCCTCTCTTTTTCGTGGCACGTACGGCTAACCACCTTCATTCCACC
601 tacgatcgtgccttattaggaaggcaacagacgggtctgacatggattggacgaaccact 660
ATGCTAGCACGGAATAATCCTTCCGTTGTCTGCCCAGACTGTACCTAACCTGCTTGGTGA
HIV-1_5_LTR other(715,895)>>>
|
661 gaattgccgcattgcagagatattgtatttaagtgcctagctcgatacataaacgggtct 720
CTTAACGGCGTAACGTCTCTATAACATAAATTCACGGATCGAGCTATGTATTTGCCCAGA
721 ctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgctt 780
GAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAA
781 aagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgac 840
TTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTG
841 tctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggc 900
AGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCACCG
901 gcccgaacagggacttgaaagcgaaagggaaaccagaggagctctctcgacgcaggactc 960
CGGGCTTGTCCCTGAACTTTCGCTTTCCCTTTGGTCTCCTCGAGAGAGCTGCGTCCTGAG
HIV-1_psi_pack other(1006,1050)>>>
|
961 ggcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaa 1020
CCGAACGACTTCGCGCGTGCCGTTCTCCGCTCCCCGCCGCTGACCACTCATGCGGTTTTT
1021 ttttgactagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcggg 1080
AAAACTGATCGCCTCCGATCTTCCTCTCTCTACCCACGCTCTCGCAGTCATAATTCGCCC
NruI
|
1081 ggagaattagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatata 1140
CCTCTTAATCTAGCGCTACCCTTTTTTAAGCCAATTCCGGTCCCCCTTTCTTTTTTATAT
1141 aattaaaacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcc 1200
TTAATTTTGTATATCATACCCGTTCGTCCCTCGATCTTGCTAAGCGTCAATTAGGACCGG
1201 tgttagaaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcaga 1260
ACAATCTTTGTAGTCTTCCGACATCTGTTTATGACCCTGTCGATGTTGGTAGGGAAGTCT
1261 caggatcagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatc 1320
GTCCTAGTCTTCTTGAATCTAGTAATATATTATGTCATCGTTGGGAGATAACACACGTAG
1321 aaaggatagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaaca 1380
TTTCCTATCTCTATTTTCTGTGGTTCCTTCGAAATCTGTTCTATCTCCTTCTCGTTTTGT
1381 aaagtaagaccaccgcacagcaagcccgctgatcttcagacctggaggaggagatatgag 1440
TTTCATTCTGGTGGCGTGTCGTTCGGGCGACTAGAAGTCTGGACCTCCTCCTCTATACTC
1441 ggacattggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagta 1500
CCTGTAACCTCTTCACTTAATATATTTATATTTCATCATTTTTAACTTGGTAATCCTCAT
RRE reg(1557,1790)>>>
|
1501 gcacccaccaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaatagga 1560
CGTGGGTGGTTCCGTTTCTCTTCTCACCACGTCTCTCTTTTTTCTCGTCACCCTTATCCT
ORF_1 rf(1)(1600,2301)>>>
|
1561 gctttgttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacg 1620
CGAAACAAGGAACCCAAGAACCCTCGTCGTCCTTCGTGATACCCGCGTCGCAGTTACTGC
1621 ctgacggtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctg 1680
GACTGCCATGTCCGGTCTGTTAATAACAGACCATATCACGTCGTCGTCTTGTTAAACGAC
1681 agggctattgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctc 1740
TCCCGATAACTCCGCGTTGTCGTAGACAACGTTGAGTGTCAGACCCCGTAGTTCGTCGAG
1741 caggcaagaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttgg 1800
GTCCGTTCTTAGGACCGACACCTTTCTATGGATTTCCTAGTTGTCGAGGACCCCTAAACC
1801 ggttgctctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaat 1860
CCAACGAGACCTTTTGAGTAAACGTGGTGACGACACGGAACCTTACGATCAACCTCATTA
1861 aaatctctggaacagatttggaatcacacgacctggatggagtgggacagagaaattaac 1920
TTTAGAGACCTTGTCTAAACCTTAGTGTGCTGGACCTACCTCACCCTGTCTCTTTAATTG
1921 aattacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaat 1980
TTAATGTGTTCGAATTATGTGAGGAATTAACTTCTTAGCGTTTTGGTCGTTCTTTTCTTA
1981 gaacaagaattattggaattagataaatgggcaagtttgtggaattggtttaacataaca 2040
CTTGTTCTTAATAACCTTAATCTATTTACCCGTTCAAACACCTTAACCAAATTGTATTGT
2041 aattggctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaaga 2100
TTAACCGACACCATATATTTTAATAAGTATTACTATCATCCTCCGAACCATCCAAATTCT
2101 atagtttttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcg 2160
TATCAAAAACGACATGAAAGATATCACTTATCTCAATCCGTCCCTATAAGTGGTAATAGC
2161 tttcagacccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaa 2220
AAAGTCTGGGTGGAGGGTTGGGGCTCCCCTGGGCTGTCCGGGCTTCCTTATCTTCTTCTT
2221 ggtggagagagagacagagacagatccattcgattagtgaacggatctcgacggtatcga 2280
CCACCTCTCTCTCTGTCTCTGTCTAGGTAAGCTAATCACTTGCCTAGAGCTGCCATAGCT
2281 aagcttgggattcgaatttaaaagaaaaggggggattggggggtacagtgcaggggaaag 2340
TTCGAACCCTAAGCTTAAATTTTCTTTTCCCCCCTAACCCCCCATGTCACGTCCCCTTTC
2341 aatagtagacataatagcaacagacatacaaactaaagaactacaaaaacaaattacaaa 2400
TTATCATCTGTATTATCGTTGTCTGTATGTTTGATTTCTTGATGTTTTTGTTTAATGTTT
2401 aattcaaaattttcgggtttttcgaacctagggttccgcgttacataacttacggtaaat 2460
TTAAGTTTTAAAAGCCCAAAAAGCTTGGATCCCAAGGCGCAATGTATTGAATGCCATTTA
2461 ggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgtt 2520
CCGGGCGGACCGACTGGCGGGTTGCTGGGGGCGGGTAACTGCAGTTATTACTGCATACAA
2521 cccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaa 2580
GGGTATCATTGCGGTTATCCCTGAAAGGTAACTGCAGTTACCCACCTCATAAATGCCATT
NdeI
|
2581 actgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtc 2640
TGACGGGTGAACCGTCATGTAGTTCACATAGTATACGGTTCATGCGGGGGATAACTGCAG
2641 aatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcct 2700
TTACTGCCATTTACCGGGCGGACCGTAATACGGGTCATGTACTGGAATACCCTGAAAGGA
2701 acttggcagtacatctacgtttagtcatcgctattaccatggtgatgcggttttggcagt 2760
TGAACCGTCATGTAGATGCAAATCAGTAGCGATAATGGTACCACTACGCCAAAACCGTCA
2761 acatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattg 2820
TGTAGTTACCCGCACCTATCGCCAAACTGAGTGCCCCTAAAGGTTCAGAGGTGGGGTAAC
2821 acgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaaca 2880
TGCAGTTACCCTCAAACAAAACCGTGGTTTTAGTTGCCCTGAAAGGTTTTACAGCATTGT
CMV prom(2885,2966)>>>
|
2881 actccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagca 2940
TGAGGCGGGGTAACTGCGTTTACCCGCCATCCGCACATGCCACCCTCCAGATATATTCGT
2941 gagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctcc 3000
CTCGAGCAAATCACTTGGCAGTCTAGCGGACCTCTGCGGTAGGTGCGACAAAACTGGAGG
tetO reg(3022,3061)>>>
|
3001 atagaagaaccgagtttaaactccctatcagtgatagagatctccctatcagtgatagag 3060
TATCTTCTTGGCTCAAATTTGAGGGATAGTCACTATCTCTAGAGGGATAGTCACTATCTC
SmaI
|
XmaI ClaI KpnI
| | | |
3061 agctagccccgggatcgatcaattgagtacttacgtaggtaccaatcagctcgctcacca 3120
TCGATCGGGGCCCTAGCTAGTTAACTCATGAATGCATCCATGGTTAGTCGAGCGAGTGGT
3121 tataacttcgtataaagtatcctatacgaagttatatcaaaataggaagaccaatgcttc 3180
ATATTGAAGCATATTTCATAGGATATGCTTCAATATAGTTTTATCCTTCTGGTTACGAAG
loxP other(3218,3251)<<<
|
3181 accatcgacccgaattgccaagcatcaccatcgacccataacttcgtataatgtatgcta 3240
TGGTAGCTGGGCTTAACGGTTCGTAGTGGTAGCTGGGTATTGAAGCATATTACATACGAT
EcoRV
|
3241 tacgaagttatctcgagtcagatatcgctagccaattgataacttcgtataggatacttt 3300
ATGCTTCAATAGAGCTCAGTCTATAGCGATCGGTTAACTATTGAAGCATATCCTATGAAA
3301 atacgaagttatcattgggattcttcctattttgatccaagcatcaccatcgaccctcta 3360
TATGCTTCAATAGTAACCCTAAGAAGGATAAAACTAGGTTCGTAGTGGTAGCTGGGAGAT
loxP other(3383,3416)>>>
|
3361 gtccagatctcaccatcgacccataacttcgtatagcatacattatacgaagttatatgg 3420
CAGGTCTAGAGTGGTAGCTGGGTATTGAAGCATATCGTATGTAATATGCTTCAATATACC
XbaI
|
3421 tgagcaagggcgattctagactgcagctcgagtacccatacgacgtcccagactacgctt 3480
ACTCGTTCCCGCTAAGATCTGACGTCGAGCTCATGGGTATGCTGCAGGGTCTGATGCGAA
SV40 prom(3497,3765)>>>
|
3481 gagtttaaacacgcgtggtgtggaaagtccccaggctccccagcaggcagaagtatgcaa 3540
CTCAAATTTGTGCGCACCACACCTTTCAGGGGTCCGAGGGGTCGTCCGTCTTCATACGTT
3541 agcatgcatctcaattagtcagcaaccaggtgtggaaagtccccaggctccccagcaggc 3600
TCGTACGTAGAGTTAATCAGTCGTTGGTCCACACCTTTCAGGGGTCCGAGGGGTCGTCCG
3601 agaagtatgcaaagcatgcatctcaattagtcagcaaccatagtcccgcccctaactccg 3660
TCTTCATACGTTTCGTACGTAGAGTTAATCAGTCGTTGGTATCAGGGCGGGGATTGAGGC
SV40 origin(3664,3741)>>>
|
3661 cccatcccgcccctaactccgcccagttccgcccattctccgccccatggctgactaatt 3720
GGGTAGGGCGGGGATTGAGGCGGGTCAAGGCGGGTAAGAGGCGGGGTACCGACTGATTAA
SfiI
|
ORF_2 rf(2)(3731,4564)>>>
| |
3721 ttttttatttatgcagaggccgaggccgcctcggcctctgagctattccagaagtagtga 3780
AAAAAATAAATACGTCTCCGGCTCCGGCGGAGCCGGAGACTCGATAAGGTCTTCATCACT
3781 ggaggcttttttggaggccgccaccAtggagagcgacgagagcggcctgcccgccatgga 3840
CCTCCGAAAAAACCTCCGGCGGTGGTACCTCTCGCTGCTCTCGCCGGACGGGCGGTACCT
3841 gatcgagtgccgcatcaccggcaccctgaacggcgtggagttcgagctggtgggcggcgg 3900
CTAGCTCACGGCGTAGTGGCCGTGGGACTTGCCGCACCTCAAGCTCGACCACCCGCCGCC
3901 agagggcacccccaagcagggccgcatgaccaacaagatgaagagcaccaaaggcgccct 3960
TCTCCCGTGGGGGTTCGTCCCGGCGTACTGGTTGTTCTACTTCTCGTGGTTTCCGCGGGA
3961 gaccttcagcccctacctgctgagccacgtgatgggctacggcttctaccacttcggcac 4020
CTGGAAGTCGGGGATGGACGACTCGGTGCACTACCCGATGCCGAAGATGGTGAAGCCGTG
4021 ctaccccagcggctacgagaaccccttcctgcacgccatcaacaacggcggctacaccaa 4080
GATGGGGTCGCCGATGCTCTTGGGGAAGGACGTGCGGTAGTTGTTGCCGCCGATGTGGTT
4081 cacccgcatcgagaagtacgaggacggcggcgtgctgcacgtgagcttcagctaccgcta 4140
GTGGGCGTAGCTCTTCATGCTCCTGCCGCCGCACGACGTGCACTCGAAGTCGATGGCGAT
4141 cgaggccggccgcgtgatcggcgacttcaaggtggtgggcaccggcttccccgaggacag 4200
GCTCCGGCCGGCGCACTAGCCGCTGAAGTTCCACCACCCGTGGCCGAAGGGGCTCCTGTC
4201 cgtgatcttcaccgacaagatcatccgcagcaacgccaccgtggagcacctgcaccccat 4260
GCACTAGAAGTGGCTGTTCTAGTAGGCGTCGTTGCGGTGGCACCTCGTGGACGTGGGGTA
4261 gggcgataacgtgctggtgggcagcttcgcccgcaccttcagcctgcgcgacggcggcta 4320
CCCGCTATTGCACGACCACCCGTCGAAGCGGGCGTGGAAGTCGGACGCGCTGCCGCCGAT
4321 ctacagcttcgtggtggacagccacatgcacttcaagagcgccatccaccccagcatcct 4380
GATGTCGAAGCACCACCTGTCGGTGTACGTGAAGTTCTCGCGGTAGGTGGGGTCGTAGGA
ApaI
|
4381 gcagaacgggggccccatgttcgccttccgccgcgtggaggagctgcacagcaacaccga 4440
CGTCTTGCCCCCGGGGTACAAGCGGAAGGCGGCGCACCTCCTCGACGTGTCGTTGTGGCT
4441 gctgggcatcgtggagtaccagcacgccttcaagacccccatcgccttcgccagatcccg 4500
CGACCCGTAGCACCTCATGGTCGTGCGGAAGTTCTGGGGGTAGCGGAAGCGGTCTAGGGC
4501 cgctcagtcgtccaattctgccgtggacggcaccgccggacccggctccaccggatctcg 4560
GCGAGTCAGCAGGTTAAGACGGCACCTGCCGTGGCGGCCTGGGCCGAGGTGGCCTAGAGC
4561 ctgaacgcgttccggaaatcaacctctggattacaaaatttgtgaaagattgactggtat 4620
GACTTGCGCAAGGCCTTTAGTTGGAGACCTAATGTTTTAAACACTTTCTAACTGACCATA
4621 tcttaactatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatca 4680
AGAATTGATACAACGAGGAAAATGCGATACACCTATGCGACGAAATTACGGAAACATAGT
4681 tgctattgcttcccgtatggctttcattttctcctccttgtataaatcctggttgctgtc 4740
ACGATAACGAAGGGCATACCGAAAGTAAAAGAGGAGGAACATATTTAGGACCAACGACAG
4741 tctttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgc 4800
AGAAATACTCCTCAACACCGGGCAACAGTCCGTTGCACCGCACCACACGTGACACAAACG
4801 tgacgcaacccccactggttggggcattgccaccacctgtcagctcctttccgggacttt 4860
ACTGCGTTGGGGGTGACCAACCCCGTAACGGTGGTGGACAGTCGAGGAAAGGCCCTGAAA
4861 cgctttccccctccctattgccacggcggaactcatcgccgcctgccttgcccgctgctg 4920
GCGAAAGGGGGAGGGATAACGGTGCCGCCTTGAGTAGCGGCGGACGGAACGGGCGACGAC
4921 gacaggggctcggctgttgggcactgacaattccgtggtgttgtcggggaagctgacgtc 4980
CTGTCCCCGAGCCGACAACCCGTGACTGTTAAGGCACCACAACAGCCCCTTCGACTGCAG
4981 ctttccatggctgctcgcctgtgttgccacctggattctgcgcgggacgtccttctgcta 5040
GAAAGGTACCGACGAGCGGACACAACGGTGGACCTAAGACGCGCCCTGCAGGAAGACGAT
5041 cgtcccttcggccctcaatccagcggaccttccttcccgcggcctgctgccggctctgcg 5100
GCAGGGAAGCCGGGAGTTAGGTCGCCTGGAAGGAAGGGCGCCGGACGACGGCCGAGACGC
5101 gcctcttccgcgtctcgccttcgccctcagacgagtcggatctccctttgggccgcctcc 5160
CGGAGAAGGCGCAGAGCGGAAGCGGGAGTCTGCTCAGCCTAGAGGGAAACCCGGCGGAGG
delta_U3 other(5174,5226)>>>
|
5161 ccgcctgtccggatggaagggctaattcactcccaacgaatacaagatctgctttttgct 5220
GGCGGACAGGCCTACCTTCCCGATTAAGTGAGGGTTGCTTATGTTCTAGACGAAAAACGA
HIV-1_5_LTR other(5227,5407)>>>
|
5221 tgtactgggtctctctggttagaccagatctgagcctgggagctctctggctaactaggg 5280
ACATGACCCAGAGAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCC
5281 aacccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgt 5340
TTGGGTGACGAATTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCA
5341 ctgttgtgtgactctggtaactagagatccctcagacccttttagtcagtgtggaaaatc 5400
GACAACACACTGAGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAG
5401 tctagcagtagtagttcatgtcatcttattattcagtatttataacttgcaaagaaatga 5460
AGATCGTCATCATCAAGTACAGTAGAATAATAAGTCATAAATATTGAACGTTTCTTTACT
5461 atatcagagagtgagaggaacttgtttattgcagcttataatggttacaaataaagcaat 5520
TATAGTCTCTCACTCTCCTTGAACAAATAACGTCGAATATTACCAATGTTTATTTCGTTA
5521 agcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtcc 5580
TCGTAGTGTTTAAAGTGTTTATTTCGTAAAAAAAGTGACGTAAGATCAACACCAAACAGG
5581 aaactcatcaatgtatcttatcatgtctggcatctatgtcgggtgcggagaaagaggtaa 5640
TTTGAGTAGTTACATAGAATAGTACAGACCGTAGATACAGCCCACGCCTCTTTCTCCATT
5641 tgaaatggcattatgggtattatgggtctgcattaatgaatcggccaacgatcccggtgt 5700
ACTTTACCGTAATACCCATAATACCCAGACGTAATTACTTAGCCGGTTGCTAGGGCCACA
5701 gaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgctcttccgcttcctcg 5760
CTTTATGGCGTGTCTACGCATTCCTCTTTTATGGCGTAGTCCGCGAGAAGGCGAAGGAGC
5761 ctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaag 5820
GAGTGACTGAGCGACGCGAGCCAGCAAGCCGACGCCGCTCGCCATAGTCGAGTGAGTTTC
5821 gcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaa 5880
CGCCATTATGCCAATAGGTGTCTTAGTCCCCTATTGCGTCCTTTCTTGTACACTCGTTTT
pBR322 origin(5913,6532)<<<
|
5881 ggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctc 5940
CCGGTCGTTTTCCGGTCCTTGGCATTTTTCCGGCGCAACGACCGCAAAAAGGTATCCGAG
5941 cgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgaca 6000
GCGGGGGGACTGCTCGTAGTGTTTTTAGCTGCGAGTTCAGTCTCCACCGCTTTGGGCTGT
6001 ggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccg 6060
CCTGATATTTCTATGGTCCGCAAAGGGGGACCTTCGAGGGAGCACGCGAGAGGACAAGGC
6061 accctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttct 6120
TGGGACGGCGAATGGCCTATGGACAGGCGGAAAGAGGGAAGCCCTTCGCACCGCGAAAGA
6121 catagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgt 6180
GTATCGAGTGCGACATCCATAGAGTCAAGCCACATCCAGCAAGCGAGGTTCGACCCGACA
6181 gtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgag 6240
CACGTGCTTGGGGGGCAAGTCGGGCTGGCGACGCGGAATAGGCCATTGATAGCAGAACTC
6241 tccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagc 6300
AGGTTGGGCCATTCTGTGCTGAATAGCGGTGACCGTCGTCGGTGACCATTGTCCTAATCG
6301 agagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctac 6360
TCTCGCTCCATACATCCGCCACGATGTCTCAAGAACTTCACCACCGGATTGATGCCGATG
6361 actagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaaga 6420
TGATCTTCCTGTCATAAACCATAGACGCGAGACGACTTCGGTCAATGGAAGCCTTTTTCT
6421 gttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgc 6480
CAACCATCGAGAACTAGGCCGTTTGTTTGGTGGCGACCATCGCCACCAAAAAAACAAACG
6481 aagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacg 6540
TTCGTCGTCTAATGCGCGTCTTTTTTTCCTAGAGTTCTTCTAGGAAACTAGAAAAGATGC
6541 gggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatca 6600
CCCAGACTGCGAGTCACCTTGCTTTTGAGTGCAATTCCCTAAAACCAGTACTCTAATAGT
6601 aaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagt 6660
TTTTCCTAGAAGTGGATCTAGGAAAATTTAATTTTTACTTCAAAATTTAGTTAGATTTCA
6661 atatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctca 6720
TATATACTCATTTGAACCAGACTGTCAATGGTTACGAATTAGTCACTCCGTGGATAGAGT
6721 gcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacg 6780
CGCTAGACAGATAAAGCAAGTAGGTATCAACGGACTGAGGGGCAGCACATCTATTGATGC
6781 atacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctca 6840
TATGCCCTCCCGAATGGTAGACCGGGGTCACGACGTTACTATGGCGCTCTGGGTGCGAGT
6841 ccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggt 6900
GGCCGAGGTCTAAATAGTCGTTATTTGGTCGGTCGGCCTTCCCGGCTCGCGTCTTCACCA
6901 cctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagt 6960
GGACGTTGAAATAGGCGGAGGTAGGTCAGATAATTAACAACGGCCCTTCGATCTCATTCA
6961 agttcgccagttaatagtttgcgcaacgttgttgaaaaaggatcttcacctagatccttt 7020
TCAAGCGGTCAATTATCAAACGCGTTGCAACAACTTTTTCCTAGAAGTGGATCTAGGAAA
7021 tcacgtagaaagccagtccgcagaaacggtgctgaccccggatgaatgtcagctactggg 7080
AGTGCATCTTTCGGTCAGGCGTCTTTGCCACGACTGGGGCCTACTTACAGTCGATGACCC
7081 ctatctggacaagggaaaacgcaagcgcaaagagaaagcaggtagcttgcagtgggctta 7140
GATAGACCTGTTCCCTTTTGCGTTCGCGTTTCTCTTTCGTCCATCGAACGTCACCCGAAT
NEOKAN prom(7185,7234)>>>
|
7141 catggcgatagctagactgggcggttttatggacagcaagcgaaccggaattgccagctg 7200
GTACCGCTATCGATCTGACCCGCCAAAATACCTGTCGTTCGCTTGGCCTTAACGGTCGAC
7201 gggcgccctctggtaaggttgggaagccctgcaaagtaaactggatggctttctcgccgc 7260
CCCGCGGGAGACCATTCCAACCCTTCGGGACGTTTCATTTGACCTACCGAAAGAGCGGCG
BclI
|
7261 caaggatctgatggcgcaggggatcaagctctgatcaagagacaggatgaggatcgtttc 7320
GTTCCTAGACTACCGCGTCCCCTAGTTCGAGACTAGTTCTCTGTCCTACTCCTAGCAAAG
NTP_II marker(7326,8114)>>>
|
ORF_3 rf(3)(7323,8117)>>>
| |
7321 gcatgattgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctat 7380
CGTACTAACTTGTTCTACCTAACGTGCGTCCAAGAGGCCGGCGAACCCACCTCTCCGATA
7381 tcggctatgactgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgt 7440
AGCCGATACTGACCCGTGTTGTCTGTTAGCCGACGAGACTACGGCGGCACAAGGCCGACA
7441 cagcgcaggggcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaac 7500
GTCGCGTCCCCGCGGGCCAAGAAAAACAGTTCTGGCTGGACAGGCCACGGGACTTACTTG
7501 tgcaagacgaggcagcgcggctatcgtggctggccacgacgggcgttccttgcgcagctg 7560
ACGTTCTGCTCCGTCGCGCCGATAGCACCGACCGGTGCTGCCCGCAAGGAACGCGTCGAC
7561 tgctcgacgttgtcactgaagcgggaagggactggctgctattgggcgaagtgccggggc 7620
ACGAGCTGCAACAGTGACTTCGCCCTTCCCTGACCGACGATAACCCGCTTCACGGCCCCG
7621 aggatctcctgtcatctcaccttgctcctgccgagaaagtatccatcatggctgatgcaa 7680
TCCTAGAGGACAGTAGAGTGGAACGAGGACGGCTCTTTCATAGGTAGTACCGACTACGTT
7681 tgcggcggctgcatacgcttgatccggctacctgcccattcgaccaccaagcgaaacatc 7740
ACGCCGCCGACGTATGCGAACTAGGCCGATGGACGGGTAAGCTGGTGGTTCGCTTTGTAG
7741 gcatcgagcgagcacgtactcggatggaagccggtcttgtcgatcaggatgatctggacg 7800
CGTAGCTCGCTCGTGCATGAGCCTACCTTCGGCCAGAACAGCTAGTCCTACTAGACCTGC
7801 aagagcatcaggggctcgcgccagccgaactgttcgccaggctcaaggcgagcatgcccg 7860
TTCTCGTAGTCCCCGAGCGCGGTCGGCTTGACAAGCGGTCCGAGTTCCGCTCGTACGGGC
7861 acggcgaggatctcgtcgtgacccatggcgatgcctgcttgccgaatatcatggtggaaa 7920
TGCCGCTCCTAGAGCAGCACTGGGTACCGCTACGGACGAACGGCTTATAGTACCACCTTT
7921 atggccgcttttctggattcatcgactgtggccggctgggtgtggcggaccgctatcagg 7980
TACCGGCGAAAAGACCTAAGTAGCTGACACCGGCCGACCCACACCGCCTGGCGATAGTCC
7981 acatagcgttggctacccgtgatattgctgaagagcttggcggcgaatgggctgaccgct 8040
TGTATCGCAACCGATGGGCACTATAACGACTTCTCGAACCGCCGCTTACCCGACTGGCGA
8041 tcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttctatcgccttc 8100
AGGAGCACGAAATGCCATAGCGGCGAGGGCTAAGCGTCGCGTAGCGGAAGATAGCGGAAG
8101 ttgacgagttcttctgaattttgttaaaatttttgttaaatcagctcattttttaaccaa 8160
AACTGCTCAAGAAGACTTAAAACAATTTTAAAAACAATTTAGTCGAGTAAAAAATTGGTT
8161 taggccgaaatcggcaacatcccttataaatcaaaagaatagaccgcgatagggttgagt 8220
ATCCGGCTTTAGCCGTTGTAGGGAATATTTAGTTTTCTTATCTGGCGCTATCCCAACTCA
f1 origin(8222,8527)<<<
|
8221 gttgttccagtttggaacaagagtccactattaaagaacgtggactccaacgtcaaaggg 8280
CAACAAGGTCAAACCTTGTTCTCAGGTGATAATTTCTTGCACCTGAGGTTGCAGTTTCCC
8281 cgaaaaaccgtctatcagggcgatggcccactacgtgaaccatcacccaaatcaagtttt 8340
GCTTTTTGGCAGATAGTCCCGCTACCGGGTGATGCACTTGGTAGTGGGTTTAGTTCAAAA
8341 ttgcggtcgaggtgccgtaaagctctaaatcggaaccctaaagggagcccccgatttaga 8400
AACGCCAGCTCCACGGCATTTCGAGATTTAGCCTTGGGATTTCCCTCGGGGGCTAAATCT
8401 gcttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcg 8460
CGAACTGCCCCTTTCGGCCGCTTGCACCGCTCTTTCCTTCCCTTCTTTCGCTTTCCTCGC
8461 ggcgctagggcgctggcaagtgtagcggtcacgctgcgcgtaaccaccacacccgcgcgc 8520
CCGCGATCCCGCGACCGTTCACATCGCCAGTGCGACGCGCATTGGTGGTGTGGGCGCGCG
8521 ttaatgcgccgctacagggcgcgtccattcgccattcaggatcgaattaattcttaatta 8580
AATTACGCGGCGATGTCCCGCGCAGGTAAGCGGTAAGTCCTAGCTTAATTAAGAATTAAT
8581 acatcatcaataatatacctt 8601
TGTAGTAGTTATTATATGGAA
LOCUS dna 8601 bp
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
misc_binding 457..462
/dbxref="REBASE:HpaI"
misc_binding 457..462
/dbxref="REBASE:HincII"
Other Gene 715..895
/gene="HIV-1_5_LTR other"
Other Gene 1006..1050
/gene="HIV-1_psi_pack other"
misc_binding 1092..1097
/dbxref="REBASE:NruI"
Regulatory_Seq 1557..1790
/gene="RRE reg"
ORF 1600..2301
/sequence="ORF_1 rf(1)"
misc_binding 2611..2616
/dbxref="REBASE:NdeI"
Promoter 2885..2966
/gene="CMV prom"
Regulatory_Seq 3022..3061
/gene="tetO reg"
misc_binding 3068..3073
/dbxref="REBASE:SmaI"
misc_binding 3068..3073
/dbxref="REBASE:XmaI"
misc_binding 3074..3079
/dbxref="REBASE:ClaI"
misc_binding 3098..3103
/dbxref="REBASE:KpnI"
Other Gene 3218..3251
/gene="loxP other"
misc_binding 3261..3266
/dbxref="REBASE:EcoRV"
Other Gene 3383..3416
/gene="loxP other"
misc_binding 3435..3440
/dbxref="REBASE:XbaI"
Promoter 3497..3765
/gene="SV40 prom"
Rep_Origin 3664..3741
/gene="SV40 origin"
ORF 3731..4564
/sequence="ORF_2 rf(2)"
misc_binding 3744..3756
/dbxref="REBASE:SfiI"
misc_binding 4390..4395
/dbxref="REBASE:ApaI"
Other Gene 5174..5226
/gene="delta_U3 other"
Other Gene 5227..5407
/gene="HIV-1_5_LTR other"
Rep_Origin 5913..6532
/gene="pBR322 origin"
Promoter 7185..7234
/gene="NEOKAN prom"
misc_binding 7292..7297
/dbxref="REBASE:BclI"
ORF 7323..8117
/sequence="ORF_3 rf(3)"
Marker 7326..8114
/gene="NTP_II marker"
Rep_Origin 8222..8527
/gene="f1 origin"
BASE COUNT 2218 a 2114 c 2256 g 2013 t 0 others