pLenti-GIII-CMV-DIO-SV40-Puro
| Vector Name | pLenti-GIII-CMV-DIO-SV40-Puro |
| VectorType | Lentiviral Vector |
| Antibiotic Information | Bacterial: Kanamycin Mammalian: Puromycin |
| Sequencing Primers | CMV sequencing primer 5`---CGC AAA TGG GCG GTA GGC GTG---3` |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta
481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag
1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga
2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag
2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa
2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat
2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag
3061 agctagcccc gggatcgatc aattgagtac ttacgtaggt accaatcagc tcgctcacca
3121 tataacttcg tataaagtat cctatacgaa gttatatcaa aataggaaga ccaatgcttc
3181 accatcgacc cgaattgcca agcatcacca tcgacccata acttcgtata atgtatgcta
3241 tacgaagtta tctcgagtca gatatcaacc actttgtaca agaaagctga acgagaaacg
3301 taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa
3361 tactgtaaaa cacaacatat ccagtcacta tggtcgacct gcagactggc tgtgtataag
3421 ggagcctgac atttatattc cccagaacat caggttaatg gcgtttttga tgtcattttc
3481 gcggtggctg agatcagcca cttcttcccc gataacggag accggcacac tggccatatc
3541 ggtggtcatc atgcgccagc tttcatcccc gatatgcacc accgggtaaa gttcacggga
3601 gactttatct gacagcagac gtgcactggc cagggggatc accatccgtc gcccgggcgt
3661 gtcaataata tcactctgta catccacaaa cagacgataa cggctctctc ttttataggt
3721 gtaaacctta aactgcattt caccagcccc tgttctcgtc agcaaaagag ccgttcattt
3781 caataaaccg ggcgacctca gccatccctt cctgattttc cgctttccag cgttcggcac
3841 gcagacgacg ggcttcattc tgcatggttg tgcttaccag accggagata ttgacatcat
3901 atatgccttg agcaactgat agctgtcgct gtcaactgtc actgtaatac gctgcttcat
3961 agcatacctc tttttgacat acttcgggta tacatatcag tatatattct tataccgcaa
4021 aaatcagcgc gcaaatacgc atactgttat ctggctttta gtaagccgga tccagatctt
4081 tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca
4141 tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg
4201 ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc
4261 acgtttaaat caaaactggt gaaactcacc cagggattgg ctgagacgaa aaacatattc
4321 tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa
4381 tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt
4441 tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca
4501 ccgtctttca ttgccatacg gaattccgga tgagcattca tcaggcgggc aagaatgtga
4561 ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata
4621 tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt
4681 tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt
4741 ttagcttcct tagctcctga aaatctcgac ggatcctaac tcaaaatcca cacattatac
4801 gagccggaag cataaagtgt aaagcctggg gtgcctaatg cggccgccat agtgactgga
4861 tatgttgtgt tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat
4921 tgatatttat atcattttac gtttctcgtt cagctttttt gtacaaactt gttgatatcg
4981 ctagccaatt gataacttcg tataggatac tttatacgaa gttatcattg ggattcttcc
5041 tattttgatc caagcatcac catcgaccct ctagtccaga tctcaccatc gacccataac
5101 ttcgtatagc atacattata cgaagttata tggtgagcaa gggcgattct agactgcagc
5161 tcgagtaccc atacgacgtc ccagactacg cttgagttta aacacgcgtg gtgtggaaag
5221 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
5281 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
5341 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
5401 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
5461 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg ccatgaccga
5521 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cctcgggccg tacgcaccct
5581 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtggacccgg accgccacat
5641 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
5701 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
5761 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
5821 ggccgcgcag caacagatgg aagggctcct ggcgccgcac cggcccaagg agcccgcgtg
5881 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
5941 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
6001 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
6061 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaacgcgttc
6121 cggaaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
6181 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
6241 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
6301 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
6361 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
6421 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
6481 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct
6541 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
6601 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
6661 tctcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctgtccgg
6721 atggaagggc taattcactc ccaacgaata caagatctgc tttttgcttg tactgggtct
6781 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
6841 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
6901 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
6961 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
7021 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
7081 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
7141 gtatcttatc atgtctggca tctatgtcgg gtgcggagaa agaggtaatg aaatggcatt
7201 atgggtatta tgggtctgca ttaatgaatc ggccaacgat cccggtgtga aataccgcac
7261 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg
7321 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
7381 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
7441 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
7501 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
7561 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
7621 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
7681 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
7741 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
7801 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
7861 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
7921 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
7981 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
8041 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
8101 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
8161 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
8221 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
8281 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
8341 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
8401 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
8461 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
8521 aatagtttgc gcaacgttgt tgaaaaagga tcttcaccta gatccttttc acgtagaaag
8581 ccagtccgca gaaacggtgc tgaccccgga tgaatgtcag ctactgggct atctggacaa
8641 gggaaaacgc aagcgcaaag agaaagcagg tagcttgcag tgggcttaca tggcgatagc
8701 tagactgggc ggttttatgg acagcaagcg aaccggaatt gccagctggg gcgccctctg
8761 gtaaggttgg gaagccctgc aaagtaaact ggatggcttt ctcgccgcca aggatctgat
8821 ggcgcagggg atcaagctct gatcaagaga caggatgagg atcgtttcgc atgattgaac
8881 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
8941 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
9001 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg
9061 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
9121 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
9181 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
9241 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
9301 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
9361 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc
9421 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
9481 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
9541 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
9601 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
9661 tctgaatttt gttaaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
9721 ggcaacatcc cttataaatc aaaagaatag accgcgatag ggttgagtgt tgttccagtt
9781 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
9841 tatcagggcg atggcccact acgtgaacca tcacccaaat caagtttttt gcggtcgagg
9901 tgccgtaaag ctctaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
9961 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
10021 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgcgcgctt aatgcgccgc
10081 tacagggcgc gtccattcgc cattcaggat cgaattaatt cttaattaac atcatcaata
10141 atatacctt
LOCUS dna 10149 bp
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
misc_binding 457..462
/dbxref="REBASE:HpaI"
Other Gene 715..895
/gene="HIV-1_5_LTR other"
Other Gene 1006..1050
/gene="HIV-1_psi_pack other"
misc_binding 1092..1097
/dbxref="REBASE:NruI"
Regulatory_Seq 1557..1790
/gene="RRE reg"
ORF 1600..2301
/sequence="ORF_1 rf(1)"
misc_binding 2611..2616
/dbxref="REBASE:NdeI"
Promoter 2885..2966
/gene="CMV prom"
Regulatory_Seq 3022..3061
/gene="tetO reg"
misc_binding 3074..3079
/dbxref="REBASE:ClaI"
misc_binding 3098..3103
/dbxref="REBASE:KpnI"
Other Gene 3218..3251
/gene="loxP other"
Other Gene 3273..3392
/gene="attR1 other"
Other Gene 3292..3392
/gene="attR2 other"
Other Gene 3433..3738
/gene="ccdB other"
Marker 4080..4739
/gene="CAT marker"
ORF 4080..4739
/sequence="ORF_4 rf(5)"
misc_binding 4521..4526
/dbxref="REBASE:EcoRI"
misc_binding 4840..4847
/dbxref="REBASE:NotI"
Other Gene 4848..4972
/gene="attR1 other"
Other Gene 4848..4948
/gene="attR2 other"
Other Gene 5096..5129
/gene="loxP other"
misc_binding 5148..5153
/dbxref="REBASE:XbaI"
Promoter 5210..5478
/gene="SV40 prom"
Rep_Origin 5377..5454
/gene="SV40 origin"
ORF 5444..6112
/sequence="ORF_2 rf(2)"
misc_binding 5457..5469
/dbxref="REBASE:SfiI"
Marker 5513..6112
/gene="puro marker"
Other Gene 6722..6774
/gene="delta_U3 other"
Other Gene 6775..6955
/gene="HIV-1_5_LTR other"
Rep_Origin 7461..8080
/gene="pBR322 origin"
Promoter 8733..8782
/gene="NEOKAN prom"
misc_binding 8840..8845
/dbxref="REBASE:BclI"
ORF 8871..9665
/sequence="ORF_3 rf(3)"
Marker 8874..9662
/gene="NTP_II marker"
Rep_Origin 9770..10075
/gene="f1 origin"
BASE COUNT 2629 a 2473 c 2585 g 2462 t 0 others
ORIGIN
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta
481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag
1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga
2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag
2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa
2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat
2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag
3061 agctagcccc gggatcgatc aattgagtac ttacgtaggt accaatcagc tcgctcacca
3121 tataacttcg tataaagtat cctatacgaa gttatatcaa aataggaaga ccaatgcttc
3181 accatcgacc cgaattgcca agcatcacca tcgacccata acttcgtata atgtatgcta
3241 tacgaagtta tctcgagtca gatatcaacc actttgtaca agaaagctga acgagaaacg
3301 taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa
3361 tactgtaaaa cacaacatat ccagtcacta tggtcgacct gcagactggc tgtgtataag
3421 ggagcctgac atttatattc cccagaacat caggttaatg gcgtttttga tgtcattttc
3481 gcggtggctg agatcagcca cttcttcccc gataacggag accggcacac tggccatatc
3541 ggtggtcatc atgcgccagc tttcatcccc gatatgcacc accgggtaaa gttcacggga
3601 gactttatct gacagcagac gtgcactggc cagggggatc accatccgtc gcccgggcgt
3661 gtcaataata tcactctgta catccacaaa cagacgataa cggctctctc ttttataggt
3721 gtaaacctta aactgcattt caccagcccc tgttctcgtc agcaaaagag ccgttcattt
3781 caataaaccg ggcgacctca gccatccctt cctgattttc cgctttccag cgttcggcac
3841 gcagacgacg ggcttcattc tgcatggttg tgcttaccag accggagata ttgacatcat
3901 atatgccttg agcaactgat agctgtcgct gtcaactgtc actgtaatac gctgcttcat
3961 agcatacctc tttttgacat acttcgggta tacatatcag tatatattct tataccgcaa
4021 aaatcagcgc gcaaatacgc atactgttat ctggctttta gtaagccgga tccagatctt
4081 tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca
4141 tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg
4201 ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc
4261 acgtttaaat caaaactggt gaaactcacc cagggattgg ctgagacgaa aaacatattc
4321 tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa
4381 tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt
4441 tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca
4501 ccgtctttca ttgccatacg gaattccgga tgagcattca tcaggcgggc aagaatgtga
4561 ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata
4621 tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt
4681 tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt
4741 ttagcttcct tagctcctga aaatctcgac ggatcctaac tcaaaatcca cacattatac
4801 gagccggaag cataaagtgt aaagcctggg gtgcctaatg cggccgccat agtgactgga
4861 tatgttgtgt tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat
4921 tgatatttat atcattttac gtttctcgtt cagctttttt gtacaaactt gttgatatcg
4981 ctagccaatt gataacttcg tataggatac tttatacgaa gttatcattg ggattcttcc
5041 tattttgatc caagcatcac catcgaccct ctagtccaga tctcaccatc gacccataac
5101 ttcgtatagc atacattata cgaagttata tggtgagcaa gggcgattct agactgcagc
5161 tcgagtaccc atacgacgtc ccagactacg cttgagttta aacacgcgtg gtgtggaaag
5221 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
5281 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
5341 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
5401 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
5461 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg ccatgaccga
5521 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cctcgggccg tacgcaccct
5581 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtggacccgg accgccacat
5641 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
5701 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
5761 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
5821 ggccgcgcag caacagatgg aagggctcct ggcgccgcac cggcccaagg agcccgcgtg
5881 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
5941 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
6001 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
6061 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaacgcgttc
6121 cggaaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
6181 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
6241 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
6301 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
6361 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
6421 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
6481 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct
6541 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
6601 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
6661 tctcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctgtccgg
6721 atggaagggc taattcactc ccaacgaata caagatctgc tttttgcttg tactgggtct
6781 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
6841 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
6901 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
6961 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
7021 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
7081 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
7141 gtatcttatc atgtctggca tctatgtcgg gtgcggagaa agaggtaatg aaatggcatt
7201 atgggtatta tgggtctgca ttaatgaatc ggccaacgat cccggtgtga aataccgcac
7261 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg
7321 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
7381 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
7441 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
7501 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
7561 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
7621 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
7681 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
7741 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
7801 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
7861 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
7921 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
7981 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
8041 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
8101 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
8161 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
8221 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
8281 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
8341 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
8401 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
8461 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
8521 aatagtttgc gcaacgttgt tgaaaaagga tcttcaccta gatccttttc acgtagaaag
8581 ccagtccgca gaaacggtgc tgaccccgga tgaatgtcag ctactgggct atctggacaa
8641 gggaaaacgc aagcgcaaag agaaagcagg tagcttgcag tgggcttaca tggcgatagc
8701 tagactgggc ggttttatgg acagcaagcg aaccggaatt gccagctggg gcgccctctg
8761 gtaaggttgg gaagccctgc aaagtaaact ggatggcttt ctcgccgcca aggatctgat
8821 ggcgcagggg atcaagctct gatcaagaga caggatgagg atcgtttcgc atgattgaac
8881 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
8941 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
9001 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg
9061 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
9121 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
9181 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
9241 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
9301 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
9361 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc
9421 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
9481 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
9541 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
9601 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
9661 tctgaatttt gttaaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
9721 ggcaacatcc cttataaatc aaaagaatag accgcgatag ggttgagtgt tgttccagtt
9781 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
9841 tatcagggcg atggcccact acgtgaacca tcacccaaat caagtttttt gcggtcgagg
9901 tgccgtaaag ctctaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
9961 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
10021 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgcgcgctt aatgcgccgc
10081 tacagggcgc gtccattcgc cattcaggat cgaattaatt cttaattaac atcatcaata
10141 atatacctt
//
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 ttttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgt 60
AAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCA
61 gggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaa 120
CCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTT
Encap other(162,310)>>>
|
121 cacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgccggtgtacacagg 180
GTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCC
181 aagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagt 240
TTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCA
241 aagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttg 300
TTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAAC
301 tgttactcatagcgcgtaatacggcagacctcagcgctagattattgaagcatttatcag 360
ACAATGAGTATCGCGCATTATGCCGTCTGGAGTCGCGATCTAATAACTTCGTAAATAGTC
amp prom(365,393)<<<
|
361 ggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggg 420
CCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCC
HpaI
|
421 gttccgcgcacatttccccgaaaagtgccacctgacgttaactataacggtcctaaggta 480
CAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAATTGATATTGCCAGGATTCCAT
481 gcgaaaatgtagtcttatgcaatactcttgtagtcttgcaacatggtaacgatgagttag 540
CGCTTTTACATCAGAATACGTTATGAGAACATCAGAACGTTGTACCATTGCTACTCAATC
541 caacatgccttacaaggagagaaaaagcaccgtgcatgccgattggtggaagtaaggtgg 600
GTTGTACGGAATGTTCCTCTCTTTTTCGTGGCACGTACGGCTAACCACCTTCATTCCACC
601 tacgatcgtgccttattaggaaggcaacagacgggtctgacatggattggacgaaccact 660
ATGCTAGCACGGAATAATCCTTCCGTTGTCTGCCCAGACTGTACCTAACCTGCTTGGTGA
HIV-1_5_LTR other(715,895)>>>
|
661 gaattgccgcattgcagagatattgtatttaagtgcctagctcgatacataaacgggtct 720
CTTAACGGCGTAACGTCTCTATAACATAAATTCACGGATCGAGCTATGTATTTGCCCAGA
721 ctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgctt 780
GAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAA
781 aagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgac 840
TTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTG
841 tctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggc 900
AGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCACCG
901 gcccgaacagggacttgaaagcgaaagggaaaccagaggagctctctcgacgcaggactc 960
CGGGCTTGTCCCTGAACTTTCGCTTTCCCTTTGGTCTCCTCGAGAGAGCTGCGTCCTGAG
HIV-1_psi_pack other(1006,1050)>>>
|
961 ggcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaa 1020
CCGAACGACTTCGCGCGTGCCGTTCTCCGCTCCCCGCCGCTGACCACTCATGCGGTTTTT
1021 ttttgactagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcggg 1080
AAAACTGATCGCCTCCGATCTTCCTCTCTCTACCCACGCTCTCGCAGTCATAATTCGCCC
NruI
|
1081 ggagaattagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatata 1140
CCTCTTAATCTAGCGCTACCCTTTTTTAAGCCAATTCCGGTCCCCCTTTCTTTTTTATAT
1141 aattaaaacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcc 1200
TTAATTTTGTATATCATACCCGTTCGTCCCTCGATCTTGCTAAGCGTCAATTAGGACCGG
1201 tgttagaaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcaga 1260
ACAATCTTTGTAGTCTTCCGACATCTGTTTATGACCCTGTCGATGTTGGTAGGGAAGTCT
1261 caggatcagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatc 1320
GTCCTAGTCTTCTTGAATCTAGTAATATATTATGTCATCGTTGGGAGATAACACACGTAG
1321 aaaggatagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaaca 1380
TTTCCTATCTCTATTTTCTGTGGTTCCTTCGAAATCTGTTCTATCTCCTTCTCGTTTTGT
1381 aaagtaagaccaccgcacagcaagcccgctgatcttcagacctggaggaggagatatgag 1440
TTTCATTCTGGTGGCGTGTCGTTCGGGCGACTAGAAGTCTGGACCTCCTCCTCTATACTC
1441 ggacattggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagta 1500
CCTGTAACCTCTTCACTTAATATATTTATATTTCATCATTTTTAACTTGGTAATCCTCAT
RRE reg(1557,1790)>>>
|
1501 gcacccaccaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaatagga 1560
CGTGGGTGGTTCCGTTTCTCTTCTCACCACGTCTCTCTTTTTTCTCGTCACCCTTATCCT
ORF_1 rf(1)(1600,2301)>>>
|
1561 gctttgttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacg 1620
CGAAACAAGGAACCCAAGAACCCTCGTCGTCCTTCGTGATACCCGCGTCGCAGTTACTGC
1621 ctgacggtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctg 1680
GACTGCCATGTCCGGTCTGTTAATAACAGACCATATCACGTCGTCGTCTTGTTAAACGAC
1681 agggctattgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctc 1740
TCCCGATAACTCCGCGTTGTCGTAGACAACGTTGAGTGTCAGACCCCGTAGTTCGTCGAG
1741 caggcaagaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttgg 1800
GTCCGTTCTTAGGACCGACACCTTTCTATGGATTTCCTAGTTGTCGAGGACCCCTAAACC
1801 ggttgctctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaat 1860
CCAACGAGACCTTTTGAGTAAACGTGGTGACGACACGGAACCTTACGATCAACCTCATTA
1861 aaatctctggaacagatttggaatcacacgacctggatggagtgggacagagaaattaac 1920
TTTAGAGACCTTGTCTAAACCTTAGTGTGCTGGACCTACCTCACCCTGTCTCTTTAATTG
1921 aattacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaat 1980
TTAATGTGTTCGAATTATGTGAGGAATTAACTTCTTAGCGTTTTGGTCGTTCTTTTCTTA
1981 gaacaagaattattggaattagataaatgggcaagtttgtggaattggtttaacataaca 2040
CTTGTTCTTAATAACCTTAATCTATTTACCCGTTCAAACACCTTAACCAAATTGTATTGT
2041 aattggctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaaga 2100
TTAACCGACACCATATATTTTAATAAGTATTACTATCATCCTCCGAACCATCCAAATTCT
2101 atagtttttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcg 2160
TATCAAAAACGACATGAAAGATATCACTTATCTCAATCCGTCCCTATAAGTGGTAATAGC
2161 tttcagacccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaa 2220
AAAGTCTGGGTGGAGGGTTGGGGCTCCCCTGGGCTGTCCGGGCTTCCTTATCTTCTTCTT
2221 ggtggagagagagacagagacagatccattcgattagtgaacggatctcgacggtatcga 2280
CCACCTCTCTCTCTGTCTCTGTCTAGGTAAGCTAATCACTTGCCTAGAGCTGCCATAGCT
2281 aagcttgggattcgaatttaaaagaaaaggggggattggggggtacagtgcaggggaaag 2340
TTCGAACCCTAAGCTTAAATTTTCTTTTCCCCCCTAACCCCCCATGTCACGTCCCCTTTC
2341 aatagtagacataatagcaacagacatacaaactaaagaactacaaaaacaaattacaaa 2400
TTATCATCTGTATTATCGTTGTCTGTATGTTTGATTTCTTGATGTTTTTGTTTAATGTTT
2401 aattcaaaattttcgggtttttcgaacctagggttccgcgttacataacttacggtaaat 2460
TTAAGTTTTAAAAGCCCAAAAAGCTTGGATCCCAAGGCGCAATGTATTGAATGCCATTTA
2461 ggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgtt 2520
CCGGGCGGACCGACTGGCGGGTTGCTGGGGGCGGGTAACTGCAGTTATTACTGCATACAA
2521 cccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaa 2580
GGGTATCATTGCGGTTATCCCTGAAAGGTAACTGCAGTTACCCACCTCATAAATGCCATT
NdeI
|
2581 actgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtc 2640
TGACGGGTGAACCGTCATGTAGTTCACATAGTATACGGTTCATGCGGGGGATAACTGCAG
2641 aatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcct 2700
TTACTGCCATTTACCGGGCGGACCGTAATACGGGTCATGTACTGGAATACCCTGAAAGGA
2701 acttggcagtacatctacgtttagtcatcgctattaccatggtgatgcggttttggcagt 2760
TGAACCGTCATGTAGATGCAAATCAGTAGCGATAATGGTACCACTACGCCAAAACCGTCA
2761 acatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattg 2820
TGTAGTTACCCGCACCTATCGCCAAACTGAGTGCCCCTAAAGGTTCAGAGGTGGGGTAAC
2821 acgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaaca 2880
TGCAGTTACCCTCAAACAAAACCGTGGTTTTAGTTGCCCTGAAAGGTTTTACAGCATTGT
CMV prom(2885,2966)>>>
|
2881 actccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagca 2940
TGAGGCGGGGTAACTGCGTTTACCCGCCATCCGCACATGCCACCCTCCAGATATATTCGT
2941 gagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctcc 3000
CTCGAGCAAATCACTTGGCAGTCTAGCGGACCTCTGCGGTAGGTGCGACAAAACTGGAGG
tetO reg(3022,3061)>>>
|
3001 atagaagaaccgagtttaaactccctatcagtgatagagatctccctatcagtgatagag 3060
TATCTTCTTGGCTCAAATTTGAGGGATAGTCACTATCTCTAGAGGGATAGTCACTATCTC
ClaI KpnI
| |
3061 agctagccccgggatcgatcaattgagtacttacgtaggtaccaatcagctcgctcacca 3120
TCGATCGGGGCCCTAGCTAGTTAACTCATGAATGCATCCATGGTTAGTCGAGCGAGTGGT
3121 tataacttcgtataaagtatcctatacgaagttatatcaaaataggaagaccaatgcttc 3180
ATATTGAAGCATATTTCATAGGATATGCTTCAATATAGTTTTATCCTTCTGGTTACGAAG
loxP other(3218,3251)<<<
|
3181 accatcgacccgaattgccaagcatcaccatcgacccataacttcgtataatgtatgcta 3240
TGGTAGCTGGGCTTAACGGTTCGTAGTGGTAGCTGGGTATTGAAGCATATTACATACGAT
attR2 other(3292,3392)<<<
|
attR1 other(3273,3392)>>>
| |
3241 tacgaagttatctcgagtcagatatcaaccactttgtacaagaaagctgaacgagaaacg 3300
ATGCTTCAATAGAGCTCAGTCTATAGTTGGTGAAACATGTTCTTTCGACTTGCTCTTTGC
3301 taaaatgatataaatatcaatatattaaattagattttgcataaaaaacagactacataa 3360
ATTTTACTATATTTATAGTTATATAATTTAATCTAAAACGTATTTTTTGTCTGATGTATT
3361 tactgtaaaacacaacatatccagtcactatggtcgacctgcagactggctgtgtataag 3420
ATGACATTTTGTGTTGTATAGGTCAGTGATACCAGCTGGACGTCTGACCGACACATATTC
ccdB other(3433,3738)<<<
|
3421 ggagcctgacatttatattccccagaacatcaggttaatggcgtttttgatgtcattttc 3480
CCTCGGACTGTAAATATAAGGGGTCTTGTAGTCCAATTACCGCAAAAACTACAGTAAAAG
3481 gcggtggctgagatcagccacttcttccccgataacggagaccggcacactggccatatc 3540
CGCCACCGACTCTAGTCGGTGAAGAAGGGGCTATTGCCTCTGGCCGTGTGACCGGTATAG
3541 ggtggtcatcatgcgccagctttcatccccgatatgcaccaccgggtaaagttcacggga 3600
CCACCAGTAGTACGCGGTCGAAAGTAGGGGCTATACGTGGTGGCCCATTTCAAGTGCCCT
3601 gactttatctgacagcagacgtgcactggccagggggatcaccatccgtcgcccgggcgt 3660
CTGAAATAGACTGTCGTCTGCACGTGACCGGTCCCCCTAGTGGTAGGCAGCGGGCCCGCA
3661 gtcaataatatcactctgtacatccacaaacagacgataacggctctctcttttataggt 3720
CAGTTATTATAGTGAGACATGTAGGTGTTTGTCTGCTATTGCCGAGAGAGAAAATATCCA
3721 gtaaaccttaaactgcatttcaccagcccctgttctcgtcagcaaaagagccgttcattt 3780
CATTTGGAATTTGACGTAAAGTGGTCGGGGACAAGAGCAGTCGTTTTCTCGGCAAGTAAA
3781 caataaaccgggcgacctcagccatcccttcctgattttccgctttccagcgttcggcac 3840
GTTATTTGGCCCGCTGGAGTCGGTAGGGAAGGACTAAAAGGCGAAAGGTCGCAAGCCGTG
3841 gcagacgacgggcttcattctgcatggttgtgcttaccagaccggagatattgacatcat 3900
CGTCTGCTGCCCGAAGTAAGACGTACCAACACGAATGGTCTGGCCTCTATAACTGTAGTA
3901 atatgccttgagcaactgatagctgtcgctgtcaactgtcactgtaatacgctgcttcat 3960
TATACGGAACTCGTTGACTATCGACAGCGACAGTTGACAGTGACATTATGCGACGAAGTA
3961 agcatacctctttttgacatacttcgggtatacatatcagtatatattcttataccgcaa 4020
TCGTATGGAGAAAAACTGTATGAAGCCCATATGTATAGTCATATATAAGAATATGGCGTT
4021 aaatcagcgcgcaaatacgcatactgttatctggcttttagtaagccggatccagatctt 4080
TTTAGTCGCGCGTTTATGCGTATGACAATAGACCGAAAATCATTCGGCCTAGGTCTAGAA
ORF_4 rf(5)(4080,4739)<<<
|
CAT marker(4080,4739)<<<
|
4081 tacgccccgccctgccactcatcgcagtactgttgtaattcattaagcattctgccgaca 4140
ATGCGGGGCGGGACGGTGAGTAGCGTCATGACAACATTAAGTAATTCGTAAGACGGCTGT
4141 tggaagccatcacaaacggcatgatgaacctgaatcgccagcggcatcagcaccttgtcg 4200
ACCTTCGGTAGTGTTTGCCGTACTACTTGGACTTAGCGGTCGCCGTAGTCGTGGAACAGC
4201 ccttgcgtataatatttgcccatggtgaaaacgggggcgaagaagttgtccatattggcc 4260
GGAACGCATATTATAAACGGGTACCACTTTTGCCCCCGCTTCTTCAACAGGTATAACCGG
4261 acgtttaaatcaaaactggtgaaactcacccagggattggctgagacgaaaaacatattc 4320
TGCAAATTTAGTTTTGACCACTTTGAGTGGGTCCCTAACCGACTCTGCTTTTTGTATAAG
4321 tcaataaaccctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaa 4380
AGTTATTTGGGAAATCCCTTTATCCGGTCCAAAAGTGGCATTGTGCGGTGTAGAACGCTT
4381 tatatgtgtagaaactgccggaaatcgtcgtggtattcactccagagcgatgaaaacgtt 4440
ATATACACATCTTTGACGGCCTTTAGCAGCACCATAAGTGAGGTCTCGCTACTTTTGCAA
4441 tcagtttgctcatggaaaacggtgtaacaagggtgaacactatcccatatcaccagctca 4500
AGTCAAACGAGTACCTTTTGCCACATTGTTCCCACTTGTGATAGGGTATAGTGGTCGAGT
EcoRI
|
4501 ccgtctttcattgccatacggaattccggatgagcattcatcaggcgggcaagaatgtga 4560
GGCAGAAAGTAACGGTATGCCTTAAGGCCTACTCGTAAGTAGTCCGCCCGTTCTTACACT
4561 ataaaggccggataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaata 4620
TATTTCCGGCCTATTTTGAACACGAATAAAAAGAAATGCCAGAAATTTTTCCGGCATTAT
4621 tccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgt 4680
AGGTCGACTTGCCAGACCAATATCCATGTAACTCGTTGACTGACTTTACGGAGTTTTACA
4681 tctttacgatgccattgggatatatcaacggtggtatatccagtgatttttttctccatt 4740
AGAAATGCTACGGTAACCCTATATAGTTGCCACCATATAGGTCACTAAAAAAAGAGGTAA
4741 ttagcttccttagctcctgaaaatctcgacggatcctaactcaaaatccacacattatac 4800
AATCGAAGGAATCGAGGACTTTTAGAGCTGCCTAGGATTGAGTTTTAGGTGTGTAATATG
attR1 other(4848,4972)<<<
|
NotI attR2 other(4848,4948)>>>
| |
4801 gagccggaagcataaagtgtaaagcctggggtgcctaatgcggccgccatagtgactgga 4860
CTCGGCCTTCGTATTTCACATTTCGGACCCCACGGATTACGCCGGCGGTATCACTGACCT
4861 tatgttgtgttttacagtattatgtagtctgttttttatgcaaaatctaatttaatatat 4920
ATACAACACAAAATGTCATAATACATCAGACAAAAAATACGTTTTAGATTAAATTATATA
4921 tgatatttatatcattttacgtttctcgttcagcttttttgtacaaacttgttgatatcg 4980
ACTATAAATATAGTAAAATGCAAAGAGCAAGTCGAAAAAACATGTTTGAACAACTATAGC
4981 ctagccaattgataacttcgtataggatactttatacgaagttatcattgggattcttcc 5040
GATCGGTTAACTATTGAAGCATATCCTATGAAATATGCTTCAATAGTAACCCTAAGAAGG
loxP other(5096,5129)>>>
|
5041 tattttgatccaagcatcaccatcgaccctctagtccagatctcaccatcgacccataac 5100
ATAAAACTAGGTTCGTAGTGGTAGCTGGGAGATCAGGTCTAGAGTGGTAGCTGGGTATTG
XbaI
|
5101 ttcgtatagcatacattatacgaagttatatggtgagcaagggcgattctagactgcagc 5160
AAGCATATCGTATGTAATATGCTTCAATATACCACTCGTTCCCGCTAAGATCTGACGTCG
SV40 prom(5210,5478)>>>
|
5161 tcgagtacccatacgacgtcccagactacgcttgagtttaaacacgcgtggtgtggaaag 5220
AGCTCATGGGTATGCTGCAGGGTCTGATGCGAACTCAAATTTGTGCGCACCACACCTTTC
5221 tccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaacc 5280
AGGGGTCCGAGGGGTCGTCCGTCTTCATACGTTTCGTACGTAGAGTTAATCAGTCGTTGG
5281 aggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaat 5340
TCCACACCTTTCAGGGGTCCGAGGGGTCGTCCGTCTTCATACGTTTCGTACGTAGAGTTA
SV40 origin(5377,5454)>>>
|
5341 tagtcagcaaccatagtcccgcccctaactccgcccatcccgcccctaactccgcccagt 5400
ATCAGTCGTTGGTATCAGGGCGGGGATTGAGGCGGGTAGGGCGGGGATTGAGGCGGGTCA
ORF_2 rf(2)(5444,6112)>>>
|
5401 tccgcccattctccgccccatggctgactaattttttttatttatgcagaggccgaggcc 5460
AGGCGGGTAAGAGGCGGGGTACCGACTGATTAAAAAAAATAAATACGTCTCCGGCTCCGG
SfiI puro marker(5513,6112)>>>
| |
5461 gcctcggcctctgagctattccagaagtagtgaggaggcttttttggaggccatgaccga 5520
CGGAGCCGGAGACTCGATAAGGTCTTCATCACTCCTCCGAAAAAACCTCCGGTACTGGCT
5521 gtacaagcccacggtgcgcctcgccacccgcgacgacgtccctcgggccgtacgcaccct 5580
CATGTTCGGGTGCCACGCGGAGCGGTGGGCGCTGCTGCAGGGAGCCCGGCATGCGTGGGA
5581 cgccgccgcgttcgccgactaccccgccacgcgccacaccgtggacccggaccgccacat 5640
GCGGCGGCGCAAGCGGCTGATGGGGCGGTGCGCGGTGTGGCACCTGGGCCTGGCGGTGTA
5641 cgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggctcgacatcggcaa 5700
GCTCGCCCAGTGGCTCGACGTTCTTGAGAAGGAGTGCGCGCAGCCCGAGCTGTAGCCGTT
5701 ggtgtgggtcgcggacgacggcgccgcggtggcggtctggaccacgccggagagcgtcga 5760
CCACACCCAGCGCCTGCTGCCGCGGCGCCACCGCCAGACCTGGTGCGGCCTCTCGCAGCT
5761 agcgggggcggtgttcgccgagatcggcccgcgcatggccgagttgagcggttcccggct 5820
TCGCCCCCGCCACAAGCGGCTCTAGCCGGGCGCGTACCGGCTCAACTCGCCAAGGGCCGA
5821 ggccgcgcagcaacagatggaagggctcctggcgccgcaccggcccaaggagcccgcgtg 5880
CCGGCGCGTCGTTGTCTACCTTCCCGAGGACCGCGGCGTGGCCGGGTTCCTCGGGCGCAC
5881 gttcctggccaccgtcggcgtctcgcccgaccaccagggcaagggtctgggcagcgccgt 5940
CAAGGACCGGTGGCAGCCGCAGAGCGGGCTGGTGGTCCCGTTCCCAGACCCGTCGCGGCA
5941 cgtgctccccggagtggaggcggccgagcgcgccggggtgcccgccttcctggagacctc 6000
GCACGAGGGGCCTCACCTCCGCCGGCTCGCGCGGCCCCACGGGCGGAAGGACCTCTGGAG
6001 cgcgccccgcaacctccccttctacgagcggctcggcttcaccgtcaccgccgacgtcga 6060
GCGCGGGGCGTTGGAGGGGAAGATGCTCGCCGAGCCGAAGTGGCAGTGGCGGCTGCAGCT
6061 ggtgcccgaaggaccgcgcacctggtgcatgacccgcaagcccggtgcctgaacgcgttc 6120
CCACGGGCTTCCTGGCGCGTGGACCACGTACTGGGCGTTCGGGCCACGGACTTGCGCAAG
6121 cggaaatcaacctctggattacaaaatttgtgaaagattgactggtattcttaactatgt 6180
GCCTTTAGTTGGAGACCTAATGTTTTAAACACTTTCTAACTGACCATAAGAATTGATACA
6181 tgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgctattgcttc 6240
ACGAGGAAAATGCGATACACCTATGCGACGAAATTACGGAAACATAGTACGATAACGAAG
6241 ccgtatggctttcattttctcctccttgtataaatcctggttgctgtctctttatgagga 6300
GGCATACCGAAAGTAAAAGAGGAGGAACATATTTAGGACCAACGACAGAGAAATACTCCT
6301 gttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccc 6360
CAACACCGGGCAACAGTCCGTTGCACCGCACCACACGTGACACAAACGACTGCGTTGGGG
6361 cactggttggggcattgccaccacctgtcagctcctttccgggactttcgctttccccct 6420
GTGACCAACCCCGTAACGGTGGTGGACAGTCGAGGAAAGGCCCTGAAAGCGAAAGGGGGA
6421 ccctattgccacggcggaactcatcgccgcctgccttgcccgctgctggacaggggctcg 6480
GGGATAACGGTGCCGCCTTGAGTAGCGGCGGACGGAACGGGCGACGACCTGTCCCCGAGC
6481 gctgttgggcactgacaattccgtggtgttgtcggggaagctgacgtcctttccatggct 6540
CGACAACCCGTGACTGTTAAGGCACCACAACAGCCCCTTCGACTGCAGGAAAGGTACCGA
6541 gctcgcctgtgttgccacctggattctgcgcgggacgtccttctgctacgtcccttcggc 6600
CGAGCGGACACAACGGTGGACCTAAGACGCGCCCTGCAGGAAGACGATGCAGGGAAGCCG
6601 cctcaatccagcggaccttccttcccgcggcctgctgccggctctgcggcctcttccgcg 6660
GGAGTTAGGTCGCCTGGAAGGAAGGGCGCCGGACGACGGCCGAGACGCCGGAGAAGGCGC
6661 tctcgccttcgccctcagacgagtcggatctccctttgggccgcctccccgcctgtccgg 6720
AGAGCGGAAGCGGGAGTCTGCTCAGCCTAGAGGGAAACCCGGCGGAGGGGCGGACAGGCC
delta_U3 other(6722,6774)>>> HIV-1_5_LTR other(6775,6955)>>>
| |
6721 atggaagggctaattcactcccaacgaatacaagatctgctttttgcttgtactgggtct 6780
TACCTTCCCGATTAAGTGAGGGTTGCTTATGTTCTAGACGAAAAACGAACATGACCCAGA
6781 ctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgctt 6840
GAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAA
6841 aagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgac 6900
TTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTG
6901 tctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtagt 6960
AGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCATCA
6961 agttcatgtcatcttattattcagtatttataacttgcaaagaaatgaatatcagagagt 7020
TCAAGTACAGTAGAATAATAAGTCATAAATATTGAACGTTTCTTTACTTATAGTCTCTCA
7021 gagaggaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaat 7080
CTCTCCTTGAACAAATAACGTCGAATATTACCAATGTTTATTTCGTTATCGTAGTGTTTA
7081 ttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaat 7140
AAGTGTTTATTTCGTAAAAAAAGTGACGTAAGATCAACACCAAACAGGTTTGAGTAGTTA
7141 gtatcttatcatgtctggcatctatgtcgggtgcggagaaagaggtaatgaaatggcatt 7200
CATAGAATAGTACAGACCGTAGATACAGCCCACGCCTCTTTCTCCATTACTTTACCGTAA
7201 atgggtattatgggtctgcattaatgaatcggccaacgatcccggtgtgaaataccgcac 7260
TACCCATAATACCCAGACGTAATTACTTAGCCGGTTGCTAGGGCCACACTTTATGGCGTG
7261 agatgcgtaaggagaaaataccgcatcaggcgctcttccgcttcctcgctcactgactcg 7320
TCTACGCATTCCTCTTTTATGGCGTAGTCCGCGAGAAGGCGAAGGAGCGAGTGACTGAGC
7321 ctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacgg 7380
GACGCGAGCCAGCAAGCCGACGCCGCTCGCCATAGTCGAGTGAGTTTCCGCCATTATGCC
7381 ttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaag 7440
AATAGGTGTCTTAGTCCCCTATTGCGTCCTTTCTTGTACACTCGTTTTCCGGTCGTTTTC
pBR322 origin(7461,8080)<<<
|
7441 gccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgac 7500
CGGTCCTTGGCATTTTTCCGGCGCAACGACCGCAAAAAGGTATCCGAGGCGGGGGGACTG
7501 gagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaaga 7560
CTCGTAGTGTTTTTAGCTGCGAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGATATTTCT
7561 taccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgctt 7620
ATGGTCCGCAAAGGGGGACCTTCGAGGGAGCACGCGAGAGGACAAGGCTGGGACGGCGAA
7621 accggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgc 7680
TGGCCTATGGACAGGCGGAAAGAGGGAAGCCCTTCGCACCGCGAAAGAGTATCGAGTGCG
7681 tgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccc 7740
ACATCCATAGAGTCAAGCCACATCCAGCAAGCGAGGTTCGACCCGACACACGTGCTTGGG
7741 cccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggta 7800
GGGCAAGTCGGGCTGGCGACGCGGAATAGGCCATTGATAGCAGAACTCAGGTTGGGCCAT
7801 agacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtat 7860
TCTGTGCTGAATAGCGGTGACCGTCGTCGGTGACCATTGTCCTAATCGTCTCGCTCCATA
7861 gtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggaca 7920
CATCCGCCACGATGTCTCAAGAACTTCACCACCGGATTGATGCCGATGTGATCTTCCTGT
7921 gtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctct 7980
CATAAACCATAGACGCGAGACGACTTCGGTCAATGGAAGCCTTTTTCTCAACCATCGAGA
7981 tgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagatt 8040
ACTAGGCCGTTTGTTTGGTGGCGACCATCGCCACCAAAAAAACAAACGTTCGTCGTCTAA
8041 acgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgct 8100
TGCGCGTCTTTTTTTCCTAGAGTTCTTCTAGGAAACTAGAAAAGATGCCCCAGACTGCGA
8101 cagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttc 8160
GTCACCTTGCTTTTGAGTGCAATTCCCTAAAACCAGTACTCTAATAGTTTTTCCTAGAAG
8161 acctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaa 8220
TGGATCTAGGAAAATTTAATTTTTACTTCAAAATTTAGTTAGATTTCATATATACTCATT
8221 acttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtcta 8280
TGAACCAGACTGTCAATGGTTACGAATTAGTCACTCCGTGGATAGAGTCGCTAGACAGAT
8281 tttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggc 8340
AAAGCAAGTAGGTATCAACGGACTGAGGGGCAGCACATCTATTGATGCTATGCCCTCCCG
8341 ttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagat 8400
AATGGTAGACCGGGGTCACGACGTTACTATGGCGCTCTGGGTGCGAGTGGCCGAGGTCTA
8401 ttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaacttta 8460
AATAGTCGTTATTTGGTCGGTCGGCCTTCCCGGCTCGCGTCTTCACCAGGACGTTGAAAT
8461 tccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagtt 8520
AGGCGGAGGTAGGTCAGATAATTAACAACGGCCCTTCGATCTCATTCATCAAGCGGTCAA
8521 aatagtttgcgcaacgttgttgaaaaaggatcttcacctagatccttttcacgtagaaag 8580
TTATCAAACGCGTTGCAACAACTTTTTCCTAGAAGTGGATCTAGGAAAAGTGCATCTTTC
8581 ccagtccgcagaaacggtgctgaccccggatgaatgtcagctactgggctatctggacaa 8640
GGTCAGGCGTCTTTGCCACGACTGGGGCCTACTTACAGTCGATGACCCGATAGACCTGTT
8641 gggaaaacgcaagcgcaaagagaaagcaggtagcttgcagtgggcttacatggcgatagc 8700
CCCTTTTGCGTTCGCGTTTCTCTTTCGTCCATCGAACGTCACCCGAATGTACCGCTATCG
NEOKAN prom(8733,8782)>>>
|
8701 tagactgggcggttttatggacagcaagcgaaccggaattgccagctggggcgccctctg 8760
ATCTGACCCGCCAAAATACCTGTCGTTCGCTTGGCCTTAACGGTCGACCCCGCGGGAGAC
8761 gtaaggttgggaagccctgcaaagtaaactggatggctttctcgccgccaaggatctgat 8820
CATTCCAACCCTTCGGGACGTTTCATTTGACCTACCGAAAGAGCGGCGGTTCCTAGACTA
NTP_II marker(8874,9662)>>>
|
BclI ORF_3 rf(3)(8871,9665)>>>
| | |
8821 ggcgcaggggatcaagctctgatcaagagacaggatgaggatcgtttcgcatgattgaac 8880
CCGCGTCCCCTAGTTCGAGACTAGTTCTCTGTCCTACTCCTAGCAAAGCGTACTAACTTG
8881 aagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggctatgact 8940
TTCTACCTAACGTGCGTCCAAGAGGCCGGCGAACCCACCTCTCCGATAAGCCGATACTGA
8941 gggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggggc 9000
CCCGTGTTGTCTGTTAGCCGACGAGACTACGGCGGCACAAGGCCGACAGTCGCGTCCCCG
9001 gcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaagacgagg 9060
CGGGCCAAGAAAAACAGTTCTGGCTGGACAGGCCACGGGACTTACTTGACGTTCTGCTCC
9061 cagcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttg 9120
GTCGCGCCGATAGCACCGACCGGTGCTGCCCGCAAGGAACGCGTCGACACGAGCTGCAAC
9121 tcactgaagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcctgt 9180
AGTGACTTCGCCCTTCCCTGACCGACGATAACCCGCTTCACGGCCCCGTCCTAGAGGACA
9181 catctcaccttgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgc 9240
GTAGAGTGGAACGAGGACGGCTCTTTCATAGGTAGTACCGACTACGTTACGCCGCCGACG
9241 atacgcttgatccggctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgag 9300
TATGCGAACTAGGCCGATGGACGGGTAAGCTGGTGGTTCGCTTTGTAGCGTAGCTCGCTC
9301 cacgtactcggatggaagccggtcttgtcgatcaggatgatctggacgaagagcatcagg 9360
GTGCATGAGCCTACCTTCGGCCAGAACAGCTAGTCCTACTAGACCTGCTTCTCGTAGTCC
9361 ggctcgcgccagccgaactgttcgccaggctcaaggcgagcatgcccgacggcgaggatc 9420
CCGAGCGCGGTCGGCTTGACAAGCGGTCCGAGTTCCGCTCGTACGGGCTGCCGCTCCTAG
9421 tcgtcgtgacccatggcgatgcctgcttgccgaatatcatggtggaaaatggccgctttt 9480
AGCAGCACTGGGTACCGCTACGGACGAACGGCTTATAGTACCACCTTTTACCGGCGAAAA
9481 ctggattcatcgactgtggccggctgggtgtggcggaccgctatcaggacatagcgttgg 9540
GACCTAAGTAGCTGACACCGGCCGACCCACACCGCCTGGCGATAGTCCTGTATCGCAACC
9541 ctacccgtgatattgctgaagagcttggcggcgaatgggctgaccgcttcctcgtgcttt 9600
GATGGGCACTATAACGACTTCTCGAACCGCCGCTTACCCGACTGGCGAAGGAGCACGAAA
9601 acggtatcgccgctcccgattcgcagcgcatcgccttctatcgccttcttgacgagttct 9660
TGCCATAGCGGCGAGGGCTAAGCGTCGCGTAGCGGAAGATAGCGGAAGAACTGCTCAAGA
9661 tctgaattttgttaaaatttttgttaaatcagctcattttttaaccaataggccgaaatc 9720
AGACTTAAAACAATTTTAAAAACAATTTAGTCGAGTAAAAAATTGGTTATCCGGCTTTAG
f1 origin(9770,10075)<<<
|
9721 ggcaacatcccttataaatcaaaagaatagaccgcgatagggttgagtgttgttccagtt 9780
CCGTTGTAGGGAATATTTAGTTTTCTTATCTGGCGCTATCCCAACTCACAACAAGGTCAA
9781 tggaacaagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtc 9840
ACCTTGTTCTCAGGTGATAATTTCTTGCACCTGAGGTTGCAGTTTCCCGCTTTTTGGCAG
9841 tatcagggcgatggcccactacgtgaaccatcacccaaatcaagttttttgcggtcgagg 9900
ATAGTCCCGCTACCGGGTGATGCACTTGGTAGTGGGTTTAGTTCAAAAAACGCCAGCTCC
9901 tgccgtaaagctctaaatcggaaccctaaagggagcccccgatttagagcttgacgggga 9960
ACGGCATTTCGAGATTTAGCCTTGGGATTTCCCTCGGGGGCTAAATCTCGAACTGCCCCT
9961 aagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcg 10020
TTCGGCCGCTTGCACCGCTCTTTCCTTCCCTTCTTTCGCTTTCCTCGCCCGCGATCCCGC
10021 ctggcaagtgtagcggtcacgctgcgcgtaaccaccacacccgcgcgcttaatgcgccgc 10080
GACCGTTCACATCGCCAGTGCGACGCGCATTGGTGGTGTGGGCGCGCGAATTACGCGGCG
10081 tacagggcgcgtccattcgccattcaggatcgaattaattcttaattaacatcatcaata 10140
ATGTCCCGCGCAGGTAAGCGGTAAGTCCTAGCTTAATTAAGAATTAATTGTAGTAGTTAT
10141 atatacctt 10149
TATATGGAA
LOCUS dna 10149 bp
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
misc_binding 457..462
/dbxref="REBASE:HpaI"
Other Gene 715..895
/gene="HIV-1_5_LTR other"
Other Gene 1006..1050
/gene="HIV-1_psi_pack other"
misc_binding 1092..1097
/dbxref="REBASE:NruI"
Regulatory_Seq 1557..1790
/gene="RRE reg"
ORF 1600..2301
/sequence="ORF_1 rf(1)"
misc_binding 2611..2616
/dbxref="REBASE:NdeI"
Promoter 2885..2966
/gene="CMV prom"
Regulatory_Seq 3022..3061
/gene="tetO reg"
misc_binding 3074..3079
/dbxref="REBASE:ClaI"
misc_binding 3098..3103
/dbxref="REBASE:KpnI"
Other Gene 3218..3251
/gene="loxP other"
Other Gene 3273..3392
/gene="attR1 other"
Other Gene 3292..3392
/gene="attR2 other"
Other Gene 3433..3738
/gene="ccdB other"
Marker 4080..4739
/gene="CAT marker"
ORF 4080..4739
/sequence="ORF_4 rf(5)"
misc_binding 4521..4526
/dbxref="REBASE:EcoRI"
misc_binding 4840..4847
/dbxref="REBASE:NotI"
Other Gene 4848..4972
/gene="attR1 other"
Other Gene 4848..4948
/gene="attR2 other"
Other Gene 5096..5129
/gene="loxP other"
misc_binding 5148..5153
/dbxref="REBASE:XbaI"
Promoter 5210..5478
/gene="SV40 prom"
Rep_Origin 5377..5454
/gene="SV40 origin"
ORF 5444..6112
/sequence="ORF_2 rf(2)"
misc_binding 5457..5469
/dbxref="REBASE:SfiI"
Marker 5513..6112
/gene="puro marker"
Other Gene 6722..6774
/gene="delta_U3 other"
Other Gene 6775..6955
/gene="HIV-1_5_LTR other"
Rep_Origin 7461..8080
/gene="pBR322 origin"
Promoter 8733..8782
/gene="NEOKAN prom"
misc_binding 8840..8845
/dbxref="REBASE:BclI"
ORF 8871..9665
/sequence="ORF_3 rf(3)"
Marker 8874..9662
/gene="NTP_II marker"
Rep_Origin 9770..10075
/gene="f1 origin"
BASE COUNT 2629 a 2473 c 2585 g 2462 t 0 others