pLenti-GIII-CMV-GFP-2A-Puro
| Vector Name | pLenti-GIII-CMV-GFP-2A-Puro |
| VectorType | Lentiviral Plasmid |
| Antibiotic Information | Bacterial: Kanamycin. Mammalian: Puromycin. |
| Sequencing Primers | CMV sequencing primer 5`---CGC AAA TGG GCG GTA GGC GTG---3` |
| Additional Information | The gene expression cassette is driven by a CMV promoter, with a GFP reporter under the control of a separate promoter |
 2023 9292bp.JPG)
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
LOCUS EMBOSS_001 9352 bp DNA linear UNC 08-MAY-2023
ORIGIN
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta
481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag
1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga
2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag
2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa
2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat
2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag
3061 agctagcgat atcaacaagt ttgtacaaaa aagttggcNN NNNNNNNNNN NNNNNNNNNN
3121 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNtg cccaactttc ttgtacaaag
3181 tggttgatat ctgaggatcc tctagaTATA AGGATGATGA CGACAAATGA GCTAGCACAT
3241 AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA CCCCCGCCCA TTGACGTCAA
3301 TAGTAACGCC AATAGGGACT TTCCATTGAC GTCAATGGGT GGAGTATTTA CGGTAAACTG
3361 CCCACTTGGC AGTACATCAA GTGTATCATA TGCCAAGTAC GCCCCCTATT GACGTCAATG
3421 ACGGTAAATG GCCCGCCTGG CATTGTGCCC AGTACATGAC CTTATGGGAC TTTCCTACTT
3481 GGCAGTACAT CTACGTATTA GTCATCGCTA TTACCATGGT CGAGGTGAGC CCCACGTTCT
3541 GCTTCACTCT CCCCATCTCC CCCCCCTCCC CACCCCCAAT TTTGTATTTA TTTATTTTTT
3601 AATTATTTTG TGCAGCGATG GGGGCGGGGG GGGGGGGGGC GCGCGCCAGG CGGGGCGGGG
3661 CGGGGCGAGG GGCGGGGCGG GGCGAGGCGG AGAGGTGCGG CGGCAGCCAA TCAGAGCGGC
3721 GCGCTCCAAA AGTTTCCTTT TATGGCGAGG CGGCGGCGGC GGCGGCCCTA TAAAAAGCGA
3781 AGCGCGCGGC GGGCGGGAGT CGCTGCGCGC TGCCTTCGCC CCGTGCCCCG CTCCGCCGCC
3841 GCCTCGCGCC GCCCGCCCCG GCTCTGACTG ACCGCGTTAC TCCCACAGGT GAGCGGGCGG
3901 GACGGCCCTT CTCCTCCGGG CTGTAATTAG CTTCGAACGC CACCATGGTG AGCAAGGGCG
3961 AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT GGACGGCGAC GTAAACGGCC
4021 ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG GCGATGCCAC CTACGGCAAG CTGACCCTGA
4081 AGTTCATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC CACCCTCGTG ACCACCTTGA
4141 CCTACGGCGT GCAGTGCTTC GCCCGCTACC CCGACCACAT GAAGCAGCAC GACTTCTTCA
4201 AGTCCGCCAT GCCCGAAGGC TACGTCCAGG AGCGCACCAT CTTCTTCAAG GACGACGGCA
4261 ACTACAAGAC CCGCGCCGAG GTGAAGTTCG AGGGCGACAC CCTGGTGAAC CGCATCGAGC
4321 TGAAGGGCAT CGACTTCAAG GAGGACGGCA ACATCCTGGG GCACAAGCTG GAGTACAACT
4381 ACAACAGCCA CAAGGTCTAT ATCACCGCCG ACAAGCAGAA GAACGGCATC AAGGTGAACT
4441 TCAAGACCCG CCACAACATC GAGGACGGCA GCGTGCAGCT CGCCGACCAC TACCAGCAGA
4501 ACACCCCCAT CGGCGACGGC CCCGTGCTGC TGCCCGACAA CCACTACCTG AGCACCCAGT
4561 CCGCCCTGAG CAAAGACCCC AACGAGAAGC GCGATCACAT GGTCCTGCTG GAGTTCGTGA
4621 CCGCCGCCGG GATCACTCTC GGCATGGACG AGCTGTACAA Ggagggcaga ggaagtcttc
4681 taacatgcgg tgacgtggag gagaatcccg gccctatgac cgagtacaag cccacggtgc
4741 gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg
4801 actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc
4861 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
4921 acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
4981 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
5041 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
5101 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
5161 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
5221 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc
5281 gcacctggtg catgacccgc aagcccggtg cctgaacgcg ttccggaaat caacctctgg
5341 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
5401 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
5461 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
5521 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
5581 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
5641 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
5701 attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca
5761 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
5821 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtctcgcc ttcgccctca
5881 gacgagtcgg atctcccttt gggccgcctc cccgcctgtc cggatggaag ggctaattca
5941 ctcccaacga atacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
6001 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
6061 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
6121 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
6181 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
6241 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
6301 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
6361 gcatctatgt cgggtgcgga gaaagaggta atgaaatggc attatgggta ttatgggtct
6421 gcattaatga atcggccaac gatcccggtg tgaaataccg cacagatgcg taaggagaaa
6481 ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
6541 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
6601 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
6661 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
6721 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
6781 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
6841 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
6901 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
6961 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
7021 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
7081 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
7141 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
7201 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
7261 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
7321 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
7381 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
7441 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
7501 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
7561 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
7621 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
7681 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
7741 tgttgaaaaa ggatcttcac ctagatcctt ttcacgtaga aagccagtcc gcagaaacgg
7801 tgctgacccc ggatgaatgt cagctactgg gctatctgga caagggaaaa cgcaagcgca
7861 aagagaaagc aggtagcttg cagtgggctt acatggcgat agctagactg ggcggtttta
7921 tggacagcaa gcgaaccgga attgccagct ggggcgccct ctggtaaggt tgggaagccc
7981 tgcaaagtaa actggatggc tttctcgccg ccaaggatct gatggcgcag gggatcaagc
8041 tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca
8101 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
8161 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
8221 aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg
8281 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
8341 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
8401 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
8461 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
8521 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
8581 ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc
8641 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
8701 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
8761 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
8821 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgaat tttgttaaaa
8881 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaca tcccttataa
8941 atcaaaagaa tagaccgcga tagggttgag tgttgttcca gtttggaaca agagtccact
9001 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
9061 actacgtgaa ccatcaccca aatcaagttt tttgcggtcg aggtgccgta aagctctaaa
9121 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
9181 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
9241 cacgctgcgc gtaaccacca cacccgcgcg cttaatgcgc cgctacaggg cgcgtccatt
9301 cgccattcag gatcgaatta attcttaatt aacatcatca ataatatacc tt
To get the full vector sequence, replace "NNN" with the protein coding sequence of your GOI.