pLenti-GIII-EF1a
| Vector Name | pLenti-GIII-EF1a |
| VectorType | Lentiviral Plasmid |
| Antibiotic Information | Bacterial: Kanamycin. Mammalian: Puromycin. |
| Sequencing Primers | EF1a sequencing primer 5'--TCA GAC AGT GGT TCA AAG--3' SV40 reverse sequencing primer 5`---TAG TCA GCC ATG GGG CGG AGA ---3` |
| Additional Information | The gene expression is driven by an EF1a promoter, without any Tag or reporter. Original Vector: 10369bp Vector without Insert Cassette: 8662bp |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
LOCUS dna 9929 bp
FEATURES Location/Qualifiers
Other Gene 109..289
/gene="HIV-1_5_LTR other"
Other Gene 400..444
/gene="HIV-1_psi_pack other"
misc_binding 486..491
/dbxref="REBASE:NruI"
Regulatory_Seq 951..1184
/gene="RRE reg"
ORF 994..1695
/sequence="ORF_1 rf(1)"
ORF 1775..2755
/sequence="ORF_5 rf(5)"
Promoter 1838..3015
/gene="EF1a prom"
misc_binding 2450..2455
/dbxref="REBASE:ApaI"
Other Gene 3041..3165
/gene="attR1 other"
Other Gene 3065..3165
/gene="attR2 other"
misc_binding 3166..3173
/dbxref="REBASE:NotI"
Marker 3274..3933
/gene="CAT marker"
ORF 3274..3933
/sequence="ORF_2 rf(1)"
misc_binding 3487..3492
/dbxref="REBASE:EcoRI"
Other Gene 4275..4580
/gene="ccdB other"
misc_binding 4356..4361
/dbxref="REBASE:SmaI"
misc_binding 4356..4361
/dbxref="REBASE:XmaI"
Other Gene 4621..4740
/gene="attR1 other"
Other Gene 4621..4721
/gene="attR2 other"
misc_binding 4762..4767
/dbxref="REBASE:XbaI"
Promoter 4824..5092
/gene="SV40 prom"
Rep_Origin 4991..5068
/gene="SV40 origin"
ORF 5058..5726
/sequence="ORF_3 rf(3)"
misc_binding 5071..5083
/dbxref="REBASE:SfiI"
Marker 5127..5726
/gene="puro marker"
Other Gene 6336..6388
/gene="delta_U3 other"
Other Gene 6389..6569
/gene="HIV-1_5_LTR other"
Rep_Origin 7076..7695
/gene="pBR322 origin"
Promoter 7953..8002
/gene="NEOKAN prom"
misc_binding 8060..8065
/dbxref="REBASE:BclI"
ORF 8091..8885
/sequence="ORF_4 rf(3)"
Marker 8094..8882
/gene="NTP_II marker"
Rep_Origin 8990..9296
/gene="f1 origin"
Other Gene 9351..9452
/gene="RITR other"
Other Gene 9351..9452
/gene="LITR other"
Other Gene 9532..9680
/gene="Encap other"
BASE COUNT 2442 a 2373 c 2742 g 2372 t 0 others
ORIGIN
1 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
61 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
121 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
181 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
241 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
301 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
361 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
421 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
481 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
541 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
601 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
661 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
721 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
781 agaccaccgc acagcaagcc cgctgatctt cagacctgga ggaggagata tgagggacat
841 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
901 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
961 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
1021 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
1081 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
1141 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
1201 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
1261 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
1321 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
1381 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
1441 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
1501 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
1561 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
1621 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgaaagctt
1681 gggattcgaa tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
1741 agacataata gcaacagaca tacaaactaa agaactacaa aaacaaatta caaaaattca
1801 aaattttcgg gtttttcgaa cctagggagt gggaattggc tccggtgccc gtcagtgggc
1861 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
1921 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
1981 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
2041 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
2101 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccactggct gcagtacgtg
2161 attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa
2221 ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg
2281 cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa
2341 aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc
2401 caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg
2461 tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg
2521 gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc
2581 ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg
2641 cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg
2701 ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac
2761 tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg
2821 tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg
2881 gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt
2941 gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca
3001 tttcaggtgt cgtgaggaat ttcgactgct agcgatatca acaagtttgt acaaaaaagc
3061 tgaacgagaa acgtaaaatg atataaatat caatatatta aattagattt tgcataaaaa
3121 acagactaca taatactgta aaacacaaca tatccagtca ctatggcggc cgcattaggc
3181 accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt gagttaggat
3241 ccgtcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc
3301 accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct
3361 caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag
3421 aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct
3481 catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac
3541 ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac
3601 cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa
3661 aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc
3721 tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc
3781 gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt
3841 caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa
3901 cagtactgcg atgagtggca gggcggggcg taaagatctg gatccggctt actaaaagcc
3961 agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata tactgatatg
4021 tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca gtgacagttg
4081 acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg gtctggtaag
4141 cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag cggaaaatca
4201 ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg ctgacgagaa
4261 caggggctgg tgaaatgcag tttaaggttt acacctataa aagagagagc cgttatcgtc
4321 tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg gtgatccccc
4381 tggccagtgc acgtctgctg tcagataaag tctcccgtga actttacccg gtggtgcata
4441 tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg gtctccgtta
4501 tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc
4561 tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct gcaggtcgac
4621 catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc
4681 taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt cttgtacaaa
4741 gtggttgata tctgaggatc ctctagactg cagctcgagt acccatacga cgtcccagac
4801 tacgcttgag tttaaacacg cgtggtgtgg aaagtcccca ggctccccag caggcagaag
4861 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
4921 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
4981 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
5041 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
5101 gtagtgagga ggcttttttg gaggccatga ccgagtacaa gcccacggtg cgcctcgcca
5161 cccgcgacga cgtccctcgg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg
5221 ccacgcgcca caccgtggac ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac
5281 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg
5341 cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg
5401 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaagggc
5461 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc
5521 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg
5581 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg
5641 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt
5701 gcatgacccg caagcccggt gcctgaacgc gttccggaaa tcaacctctg gattacaaaa
5761 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
5821 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
5881 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
5941 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
6001 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
6061 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
6121 tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc
6181 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
6241 gcggcctgct gccggctctg cggcctcttc cgcgtctcgc cttcgccctc agacgagtcg
6301 gatctccctt tgggccgcct ccccgcctgt ccggatggaa gggctaattc actcccaacg
6361 aatacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
6421 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
6481 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
6541 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
6601 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta
6661 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
6721 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggcatctatg
6781 tcgggtgcgg agaaagaggt aatgaaatgg cattatgggt attatgggtc tgcattaatg
6841 aatcggccaa cggatgtcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
6901 tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
6961 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
7021 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
7081 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
7141 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
7201 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
7261 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
7321 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
7381 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
7441 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
7501 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
7561 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
7621 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
7681 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
7741 ggattttggc catgagatta tcaaaaagga tcttcaccta gatccttttc acgtagaaag
7801 ccagtccgca gaaacggtgc tgaccccgga tgaatgtcag ctactgggct atctggacaa
7861 gggaaaacgc aagcgcaaag agaaagcagg tagcttgcag tgggcttaca tggcgatagc
7921 tagactgggc ggttttatgg acagcaagcg aaccggaatt gccagctggg gcgccctctg
7981 gtaaggttgg gaagccctgc aaagtaaact ggatggcttt cttgccgcca aggatctgat
8041 ggcgcagggg atcaagctct gatcaagaga caggatgagg atcgtttcgc atgattgaac
8101 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
8161 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
8221 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg
8281 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
8341 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
8401 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
8461 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
8521 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
8581 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc
8641 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
8701 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
8761 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
8821 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
8881 tctgaatttt gttaaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
8941 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
9001 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
9061 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg
9121 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
9181 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
9241 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
9301 ctacagggcg cgtccattcg ccattcagga tcgaattaat tcttaattaa catcatcaat
9361 aatatacctt ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg
9421 cgcggggcgt gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag
9481 tgtggcggaa cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg
9541 tgtacacagg aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc
9601 gtaaccgagt aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg
9661 aataattttg tgttactcat agcgcgtaat actctcatga gcggatacat atttgaatgt
9721 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
9781 gtaactataa cggtcctaag gtagcgaaaa tgtagtctta tgcaatactc ttgtagtctt
9841 gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat
9901 gccgattggt ggaagtaagg tggtacgat
//
LOCUS dna 9929 bp
FEATURES Location/Qualifiers
Other Gene 109..289
/gene="HIV-1_5_LTR other"
Other Gene 400..444
/gene="HIV-1_psi_pack other"
misc_binding 486..491
/dbxref="REBASE:NruI"
Regulatory_Seq 951..1184
/gene="RRE reg"
ORF 994..1695
/sequence="ORF_1 rf(1)"
ORF 1775..2755
/sequence="ORF_5 rf(5)"
Promoter 1838..3015
/gene="EF1a prom"
misc_binding 2450..2455
/dbxref="REBASE:ApaI"
Other Gene 3041..3165
/gene="attR1 other"
Other Gene 3065..3165
/gene="attR2 other"
misc_binding 3166..3173
/dbxref="REBASE:NotI"
Marker 3274..3933
/gene="CAT marker"
ORF 3274..3933
/sequence="ORF_2 rf(1)"
misc_binding 3487..3492
/dbxref="REBASE:EcoRI"
Other Gene 4275..4580
/gene="ccdB other"
misc_binding 4356..4361
/dbxref="REBASE:SmaI"
misc_binding 4356..4361
/dbxref="REBASE:XmaI"
Other Gene 4621..4740
/gene="attR1 other"
Other Gene 4621..4721
/gene="attR2 other"
misc_binding 4762..4767
/dbxref="REBASE:XbaI"
Promoter 4824..5092
/gene="SV40 prom"
Rep_Origin 4991..5068
/gene="SV40 origin"
ORF 5058..5726
/sequence="ORF_3 rf(3)"
misc_binding 5071..5083
/dbxref="REBASE:SfiI"
Marker 5127..5726
/gene="puro marker"
Other Gene 6336..6388
/gene="delta_U3 other"
Other Gene 6389..6569
/gene="HIV-1_5_LTR other"
Rep_Origin 7076..7695
/gene="pBR322 origin"
Promoter 7953..8002
/gene="NEOKAN prom"
misc_binding 8060..8065
/dbxref="REBASE:BclI"
ORF 8091..8885
/sequence="ORF_4 rf(3)"
Marker 8094..8882
/gene="NTP_II marker"
Rep_Origin 8990..9296
/gene="f1 origin"
Other Gene 9351..9452
/gene="RITR other"
Other Gene 9351..9452
/gene="LITR other"
Other Gene 9532..9680
/gene="Encap other"
BASE COUNT 2442 a 2373 c 2742 g 2372 t 0 others
ORIGIN
1 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
61 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
121 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
181 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
241 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
301 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
361 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
421 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
481 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
541 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
601 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
661 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
721 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
781 agaccaccgc acagcaagcc cgctgatctt cagacctgga ggaggagata tgagggacat
841 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
901 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
961 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
1021 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
1081 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
1141 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
1201 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
1261 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
1321 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
1381 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
1441 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
1501 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
1561 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
1621 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgaaagctt
1681 gggattcgaa tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
1741 agacataata gcaacagaca tacaaactaa agaactacaa aaacaaatta caaaaattca
1801 aaattttcgg gtttttcgaa cctagggagt gggaattggc tccggtgccc gtcagtgggc
1861 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
1921 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
1981 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
2041 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
2101 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccactggct gcagtacgtg
2161 attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa
2221 ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg
2281 cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa
2341 aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc
2401 caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg
2461 tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg
2521 gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc
2581 ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg
2641 cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg
2701 ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac
2761 tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg
2821 tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg
2881 gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt
2941 gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca
3001 tttcaggtgt cgtgaggaat ttcgactgct agcgatatca acaagtttgt acaaaaaagc
3061 tgaacgagaa acgtaaaatg atataaatat caatatatta aattagattt tgcataaaaa
3121 acagactaca taatactgta aaacacaaca tatccagtca ctatggcggc cgcattaggc
3181 accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt gagttaggat
3241 ccgtcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc
3301 accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct
3361 caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag
3421 aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct
3481 catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac
3541 ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac
3601 cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa
3661 aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc
3721 tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc
3781 gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt
3841 caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa
3901 cagtactgcg atgagtggca gggcggggcg taaagatctg gatccggctt actaaaagcc
3961 agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata tactgatatg
4021 tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca gtgacagttg
4081 acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg gtctggtaag
4141 cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag cggaaaatca
4201 ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg ctgacgagaa
4261 caggggctgg tgaaatgcag tttaaggttt acacctataa aagagagagc cgttatcgtc
4321 tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg gtgatccccc
4381 tggccagtgc acgtctgctg tcagataaag tctcccgtga actttacccg gtggtgcata
4441 tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg gtctccgtta
4501 tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc
4561 tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct gcaggtcgac
4621 catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc
4681 taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt cttgtacaaa
4741 gtggttgata tctgaggatc ctctagactg cagctcgagt acccatacga cgtcccagac
4801 tacgcttgag tttaaacacg cgtggtgtgg aaagtcccca ggctccccag caggcagaag
4861 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
4921 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
4981 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
5041 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
5101 gtagtgagga ggcttttttg gaggccatga ccgagtacaa gcccacggtg cgcctcgcca
5161 cccgcgacga cgtccctcgg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg
5221 ccacgcgcca caccgtggac ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac
5281 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg
5341 cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg
5401 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaagggc
5461 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc
5521 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg
5581 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg
5641 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt
5701 gcatgacccg caagcccggt gcctgaacgc gttccggaaa tcaacctctg gattacaaaa
5761 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
5821 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
5881 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
5941 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
6001 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
6061 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
6121 tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc
6181 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
6241 gcggcctgct gccggctctg cggcctcttc cgcgtctcgc cttcgccctc agacgagtcg
6301 gatctccctt tgggccgcct ccccgcctgt ccggatggaa gggctaattc actcccaacg
6361 aatacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
6421 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
6481 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
6541 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
6601 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta
6661 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
6721 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggcatctatg
6781 tcgggtgcgg agaaagaggt aatgaaatgg cattatgggt attatgggtc tgcattaatg
6841 aatcggccaa cggatgtcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
6901 tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
6961 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
7021 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
7081 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
7141 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
7201 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
7261 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
7321 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
7381 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
7441 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
7501 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
7561 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
7621 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
7681 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
7741 ggattttggc catgagatta tcaaaaagga tcttcaccta gatccttttc acgtagaaag
7801 ccagtccgca gaaacggtgc tgaccccgga tgaatgtcag ctactgggct atctggacaa
7861 gggaaaacgc aagcgcaaag agaaagcagg tagcttgcag tgggcttaca tggcgatagc
7921 tagactgggc ggttttatgg acagcaagcg aaccggaatt gccagctggg gcgccctctg
7981 gtaaggttgg gaagccctgc aaagtaaact ggatggcttt cttgccgcca aggatctgat
8041 ggcgcagggg atcaagctct gatcaagaga caggatgagg atcgtttcgc atgattgaac
8101 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
8161 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
8221 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg
8281 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
8341 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
8401 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
8461 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
8521 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
8581 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc
8641 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
8701 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
8761 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
8821 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
8881 tctgaatttt gttaaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
8941 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
9001 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
9061 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg
9121 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
9181 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
9241 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
9301 ctacagggcg cgtccattcg ccattcagga tcgaattaat tcttaattaa catcatcaat
9361 aatatacctt ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg
9421 cgcggggcgt gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag
9481 tgtggcggaa cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg
9541 tgtacacagg aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc
9601 gtaaccgagt aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg
9661 aataattttg tgttactcat agcgcgtaat actctcatga gcggatacat atttgaatgt
9721 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
9781 gtaactataa cggtcctaag gtagcgaaaa tgtagtctta tgcaatactc ttgtagtctt
9841 gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat
9901 gccgattggt ggaagtaagg tggtacgat
//
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 cgtgccttattaggaaggcaacagacgggtctgacatggattggacgaaccactgaattg 60
GCACGGAATAATCCTTCCGTTGTCTGCCCAGACTGTACCTAACCTGCTTGGTGACTTAAC
HIV-1_5_LTR other(109,289)>>>
|
61 ccgcattgcagagatattgtatttaagtgcctagctcgatacataaacgggtctctctgg 120
GGCGTAACGTCTCTATAACATAAATTCACGGATCGAGCTATGTATTTGCCCAGAGAGACC
121 ttagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcct 180
AATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAATTCGGA
181 caataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggt 240
GTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTGAGACCA
241 aactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggcgcccga 300
TTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCACCGCGGGCT
301 acagggacttgaaagcgaaagggaaaccagaggagctctctcgacgcaggactcggcttg 360
TGTCCCTGAACTTTCGCTTTCCCTTTGGTCTCCTCGAGAGAGCTGCGTCCTGAGCCGAAC
HIV-1_psi_pack other(400,444)>>>
|
361 ctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaattttga 420
GACTTCGCGCGTGCCGTTCTCCGCTCCCCGCCGCTGACCACTCATGCGGTTTTTAAAACT
421 ctagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcgggggagaa 480
GATCGCCTCCGATCTTCCTCTCTCTACCCACGCTCTCGCAGTCATAATTCGCCCCCTCTT
NruI
|
481 ttagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatataaattaa 540
AATCTAGCGCTACCCTTTTTTAAGCCAATTCCGGTCCCCCTTTCTTTTTTATATTTAATT
541 aacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcctgttag 600
TTGTATATCATACCCGTTCGTCCCTCGATCTTGCTAAGCGTCAATTAGGACCGGACAATC
601 aaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcagacaggat 660
TTTGTAGTCTTCCGACATCTGTTTATGACCCTGTCGATGTTGGTAGGGAAGTCTGTCCTA
661 cagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatcaaagga 720
GTCTTCTTGAATCTAGTAATATATTATGTCATCGTTGGGAGATAACACACGTAGTTTCCT
721 tagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaacaaaagta 780
ATCTCTATTTTCTGTGGTTCCTTCGAAATCTGTTCTATCTCCTTCTCGTTTTGTTTTCAT
781 agaccaccgcacagcaagcccgctgatcttcagacctggaggaggagatatgagggacat 840
TCTGGTGGCGTGTCGTTCGGGCGACTAGAAGTCTGGACCTCCTCCTCTATACTCCCTGTA
841 tggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagtagcaccc 900
ACCTCTTCACTTAATATATTTATATTTCATCATTTTTAACTTGGTAATCCTCATCGTGGG
RRE reg(951,1184)>>>
|
901 accaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaataggagctttg 960
TGGTTCCGTTTCTCTTCTCACCACGTCTCTCTTTTTTCTCGTCACCCTTATCCTCGAAAC
ORF_1 rf(1)(994,1695)>>>
|
961 ttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacgctgacg 1020
AAGGAACCCAAGAACCCTCGTCGTCCTTCGTGATACCCGCGTCGCAGTTACTGCGACTGC
1021 gtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctgagggct 1080
CATGTCCGGTCTGTTAATAACAGACCATATCACGTCGTCGTCTTGTTAAACGACTCCCGA
1081 attgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctccaggca 1140
TAACTCCGCGTTGTCGTAGACAACGTTGAGTGTCAGACCCCGTAGTTCGTCGAGGTCCGT
1141 agaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttggggttgc 1200
TCTTAGGACCGACACCTTTCTATGGATTTCCTAGTTGTCGAGGACCCCTAAACCCCAACG
1201 tctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaataaatct 1260
AGACCTTTTGAGTAAACGTGGTGACGACACGGAACCTTACGATCAACCTCATTATTTAGA
1261 ctggaacagatttggaatcacacgacctggatggagtgggacagagaaattaacaattac 1320
GACCTTGTCTAAACCTTAGTGTGCTGGACCTACCTCACCCTGTCTCTTTAATTGTTAATG
1321 acaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaatgaacaa 1380
TGTTCGAATTATGTGAGGAATTAACTTCTTAGCGTTTTGGTCGTTCTTTTCTTACTTGTT
1381 gaattattggaattagataaatgggcaagtttgtggaattggtttaacataacaaattgg 1440
CTTAATAACCTTAATCTATTTACCCGTTCAAACACCTTAACCAAATTGTATTGTTTAACC
1441 ctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaagaatagtt 1500
GACACCATATATTTTAATAAGTATTACTATCATCCTCCGAACCATCCAAATTCTTATCAA
1501 tttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcgtttcag 1560
AAACGACATGAAAGATATCACTTATCTCAATCCGTCCCTATAAGTGGTAATAGCAAAGTC
1561 acccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaaggtgga 1620
TGGGTGGAGGGTTGGGGCTCCCCTGGGCTGTCCGGGCTTCCTTATCTTCTTCTTCCACCT
1621 gagagagacagagacagatccattcgattagtgaacggatctcgacggtatcgaaagctt 1680
CTCTCTCTGTCTCTGTCTAGGTAAGCTAATCACTTGCCTAGAGCTGCCATAGCTTTCGAA
1681 gggattcgaatttaaaagaaaaggggggattggggggtacagtgcaggggaaagaatagt 1740
CCCTAAGCTTAAATTTTCTTTTCCCCCCTAACCCCCCATGTCACGTCCCCTTTCTTATCA
ORF_5 rf(5)(1775,2755)<<<
|
1741 agacataatagcaacagacatacaaactaaagaactacaaaaacaaattacaaaaattca 1800
TCTGTATTATCGTTGTCTGTATGTTTGATTTCTTGATGTTTTTGTTTAATGTTTTTAAGT
EF1a prom(1838,3015)>>>
|
1801 aaattttcgggtttttcgaacctagggagtgggaattggctccggtgcccgtcagtgggc 1860
TTTAAAAGCCCAAAAAGCTTGGATCCCTCACCCTTAACCGAGGCCACGGGCAGTCACCCG
1861 agagcgcacatcgcccacagtccccgagaagttggggggaggggtcggcaattgaaccgg 1920
TCTCGCGTGTAGCGGGTGTCAGGGGCTCTTCAACCCCCCTCCCCAGCCGTTAACTTGGCC
1921 tgcctagagaaggtggcgcggggtaaactgggaaagtgatgtcgtgtactggctccgcct 1980
ACGGATCTCTTCCACCGCGCCCCATTTGACCCTTTCACTACAGCACATGACCGAGGCGGA
1981 ttttcccgagggtgggggagaaccgtatataagtgcagtagtcgccgtgaacgttctttt 2040
AAAAGGGCTCCCACCCCCTCTTGGCATATATTCACGTCATCAGCGGCACTTGCAAGAAAA
2041 tcgcaacgggtttgccgccagaacacaggtaagtgccgtgtgtggttcccgcgggcctgg 2100
AGCGTTGCCCAAACGGCGGTCTTGTGTCCATTCACGGCACACACCAAGGGCGCCCGGACC
2101 cctctttacgggttatggcccttgcgtgccttgaattacttccactggctgcagtacgtg 2160
GGAGAAATGCCCAATACCGGGAACGCACGGAACTTAATGAAGGTGACCGACGTCATGCAC
2161 attcttgatcccgagcttcgggttggaagtgggtgggagagttcgaggccttgcgcttaa 2220
TAAGAACTAGGGCTCGAAGCCCAACCTTCACCCACCCTCTCAAGCTCCGGAACGCGAATT
2221 ggagccccttcgcctcgtgcttgagttgaggcctggcctgggcgctggggccgccgcgtg 2280
CCTCGGGGAAGCGGAGCACGAACTCAACTCCGGACCGGACCCGCGACCCCGGCGGCGCAC
2281 cgaatctggtggcaccttcgcgcctgtctcgctgctttcgataagtctctagccatttaa 2340
GCTTAGACCACCGTGGAAGCGCGGACAGAGCGACGAAAGCTATTCAGAGATCGGTAAATT
2341 aatttttgatgacctgctgcgacgctttttttctggcaagatagtcttgtaaatgcgggc 2400
TTAAAAACTACTGGACGACGCTGCGAAAAAAAGACCGTTCTATCAGAACATTTACGCCCG
ApaI
|
2401 caagatctgcacactggtatttcggtttttggggccgcgggcggcgacggggcccgtgcg 2460
GTTCTAGACGTGTGACCATAAAGCCAAAAACCCCGGCGCCCGCCGCTGCCCCGGGCACGC
2461 tcccagcgcacatgttcggcgaggcggggcctgcgagcgcggccaccgagaatcggacgg 2520
AGGGTCGCGTGTACAAGCCGCTCCGCCCCGGACGCTCGCGCCGGTGGCTCTTAGCCTGCC
2521 gggtagtctcaagctggccggcctgctctggtgcctggcctcgcgccgccgtgtatcgcc 2580
CCCATCAGAGTTCGACCGGCCGGACGAGACCACGGACCGGAGCGCGGCGGCACATAGCGG
2581 ccgccctgggcggcaaggctggcccggtcggcaccagttgcgtgagcggaaagatggccg 2640
GGCGGGACCCGCCGTTCCGACCGGGCCAGCCGTGGTCAACGCACTCGCCTTTCTACCGGC
2641 cttcccggccctgctgcagggagctcaaaatggaggacgcggcgctcgggagagcgggcg 2700
GAAGGGCCGGGACGACGTCCCTCGAGTTTTACCTCCTGCGCCGCGAGCCCTCTCGCCCGC
2701 ggtgagtcacccacacaaaggaaaagggcctttccgtcctcagccgtcgcttcatgtgac 2760
CCACTCAGTGGGTGTGTTTCCTTTTCCCGGAAAGGCAGGAGTCGGCAGCGAAGTACACTG
2761 tccacggagtaccgggcgccgtccaggcacctcgattagttctcgagcttttggagtacg 2820
AGGTGCCTCATGGCCCGCGGCAGGTCCGTGGAGCTAATCAAGAGCTCGAAAACCTCATGC
2821 tcgtctttaggttggggggaggggttttatgcgatggagtttccccacactgagtgggtg 2880
AGCAGAAATCCAACCCCCCTCCCCAAAATACGCTACCTCAAAGGGGTGTGACTCACCCAC
2881 gagactgaagttaggccagcttggcacttgatgtaattctccttggaatttgcccttttt 2940
CTCTGACTTCAATCCGGTCGAACCGTGAACTACATTAAGAGGAACCTTAAACGGGAAAAA
2941 gagtttggatcttggttcattctcaagcctcagacagtggttcaaagtttttttcttcca 3000
CTCAAACCTAGAACCAAGTAAGAGTTCGGAGTCTGTCACCAAGTTTCAAAAAAAGAAGGT
attR1 other(3041,3165)>>>
|
3001 tttcaggtgtcgtgaggaatttcgactgctagcgatatcaacaagtttgtacaaaaaagc 3060
AAAGTCCACAGCACTCCTTAAAGCTGACGATCGCTATAGTTGTTCAAACATGTTTTTTCG
attR2 other(3065,3165)<<<
|
3061 tgaacgagaaacgtaaaatgatataaatatcaatatattaaattagattttgcataaaaa 3120
ACTTGCTCTTTGCATTTTACTATATTTATAGTTATATAATTTAATCTAAAACGTATTTTT
NotI
|
3121 acagactacataatactgtaaaacacaacatatccagtcactatggcggccgcattaggc 3180
TGTCTGATGTATTATGACATTTTGTGTTGTATAGGTCAGTGATACCGCCGGCGTAATCCG
3181 accccaggctttacactttatgcttccggctcgtataatgtgtggattttgagttaggat 3240
TGGGGTCCGAAATGTGAAATACGAAGGCCGAGCATATTACACACCTAAAACTCAATCCTA
CAT marker(3274,3933)>>>
|
ORF_2 rf(1)(3274,3933)>>>
|
3241 ccgtcgagattttcaggagctaaggaagctaaaatggagaaaaaaatcactggatatacc 3300
GGCAGCTCTAAAAGTCCTCGATTCCTTCGATTTTACCTCTTTTTTTAGTGACCTATATGG
3301 accgttgatatatcccaatggcatcgtaaagaacattttgaggcatttcagtcagttgct 3360
TGGCAACTATATAGGGTTACCGTAGCATTTCTTGTAAAACTCCGTAAAGTCAGTCAACGA
3361 caatgtacctataaccagaccgttcagctggatattacggcctttttaaagaccgtaaag 3420
GTTACATGGATATTGGTCTGGCAAGTCGACCTATAATGCCGGAAAAATTTCTGGCATTTC
3421 aaaaataagcacaagttttatccggcctttattcacattcttgcccgcctgatgaatgct 3480
TTTTTATTCGTGTTCAAAATAGGCCGGAAATAAGTGTAAGAACGGGCGGACTACTTACGA
EcoRI
|
3481 catccggaattccgtatggcaatgaaagacggtgagctggtgatatgggatagtgttcac 3540
GTAGGCCTTAAGGCATACCGTTACTTTCTGCCACTCGACCACTATACCCTATCACAAGTG
3541 ccttgttacaccgttttccatgagcaaactgaaacgttttcatcgctctggagtgaatac 3600
GGAACAATGTGGCAAAAGGTACTCGTTTGACTTTGCAAAAGTAGCGAGACCTCACTTATG
3601 cacgacgatttccggcagtttctacacatatattcgcaagatgtggcgtgttacggtgaa 3660
GTGCTGCTAAAGGCCGTCAAAGATGTGTATATAAGCGTTCTACACCGCACAATGCCACTT
3661 aacctggcctatttccctaaagggtttattgagaatatgtttttcgtctcagccaatccc 3720
TTGGACCGGATAAAGGGATTTCCCAAATAACTCTTATACAAAAAGCAGAGTCGGTTAGGG
3721 tgggtgagtttcaccagttttgatttaaacgtggccaatatggacaacttcttcgccccc 3780
ACCCACTCAAAGTGGTCAAAACTAAATTTGCACCGGTTATACCTGTTGAAGAAGCGGGGG
3781 gttttcaccatgggcaaatattatacgcaaggcgacaaggtgctgatgccgctggcgatt 3840
CAAAAGTGGTACCCGTTTATAATATGCGTTCCGCTGTTCCACGACTACGGCGACCGCTAA
3841 caggttcatcatgccgtttgtgatggcttccatgtcggcagaatgcttaatgaattacaa 3900
GTCCAAGTAGTACGGCAAACACTACCGAAGGTACAGCCGTCTTACGAATTACTTAATGTT
3901 cagtactgcgatgagtggcagggcggggcgtaaagatctggatccggcttactaaaagcc 3960
GTCATGACGCTACTCACCGTCCCGCCCCGCATTTCTAGACCTAGGCCGAATGATTTTCGG
3961 agataacagtatgcgtatttgcgcgctgatttttgcggtataagaatatatactgatatg 4020
TCTATTGTCATACGCATAAACGCGCGACTAAAAACGCCATATTCTTATATATGACTATAC
4021 tatacccgaagtatgtcaaaaagaggtatgctatgaagcagcgtattacagtgacagttg 4080
ATATGGGCTTCATACAGTTTTTCTCCATACGATACTTCGTCGCATAATGTCACTGTCAAC
4081 acagcgacagctatcagttgctcaaggcatatatgatgtcaatatctccggtctggtaag 4140
TGTCGCTGTCGATAGTCAACGAGTTCCGTATATACTACAGTTATAGAGGCCAGACCATTC
4141 cacaaccatgcagaatgaagcccgtcgtctgcgtgccgaacgctggaaagcggaaaatca 4200
GTGTTGGTACGTCTTACTTCGGGCAGCAGACGCACGGCTTGCGACCTTTCGCCTTTTAGT
4201 ggaagggatggctgaggtcgcccggtttattgaaatgaacggctcttttgctgacgagaa 4260
CCTTCCCTACCGACTCCAGCGGGCCAAATAACTTTACTTGCCGAGAAAACGACTGCTCTT
ccdB other(4275,4580)>>>
|
4261 caggggctggtgaaatgcagtttaaggtttacacctataaaagagagagccgttatcgtc 4320
GTCCCCGACCACTTTACGTCAAATTCCAAATGTGGATATTTTCTCTCTCGGCAATAGCAG
SmaI
|
XmaI
| |
4321 tgtttgtggatgtacagagtgatattattgacacgcccgggcgacggatggtgatccccc 4380
ACAAACACCTACATGTCTCACTATAATAACTGTGCGGGCCCGCTGCCTACCACTAGGGGG
4381 tggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcata 4440
ACCGGTCACGTGCAGACGACAGTCTATTTCAGAGGGCACTTGAAATGGGCCACCACGTAT
4441 tcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgtta 4500
AGCCCCTACTTTCGACCGCGTACTACTGGTGGCTATACCGGTCACACGGCCAGAGGCAAT
4501 tcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacc 4560
AGCCCCTTCTTCACCGACTAGAGTCGGTGGCGCTTTTACTGTAGTTTTTGCGGTAATTGG
4561 tgatgttctggggaatataaatgtcaggctcccttatacacagccagtctgcaggtcgac 4620
ACTACAAGACCCCTTATATTTACAGTCCGAGGGAATATGTGTCGGTCAGACGTCCAGCTG
attR2 other(4621,4721)>>>
|
attR1 other(4621,4740)<<<
|
4621 catagtgactggatatgttgtgttttacagtattatgtagtctgttttttatgcaaaatc 4680
GTATCACTGACCTATACAACACAAAATGTCATAATACATCAGACAAAAAATACGTTTTAG
4681 taatttaatatattgatatttatatcattttacgtttctcgttcagctttcttgtacaaa 4740
ATTAAATTATATAACTATAAATATAGTAAAATGCAAAGAGCAAGTCGAAAGAACATGTTT
XbaI
|
4741 gtggttgatatctgaggatcctctagactgcagctcgagtacccatacgacgtcccagac 4800
CACCAACTATAGACTCCTAGGAGATCTGACGTCGAGCTCATGGGTATGCTGCAGGGTCTG
SV40 prom(4824,5092)>>>
|
4801 tacgcttgagtttaaacacgcgtggtgtggaaagtccccaggctccccagcaggcagaag 4860
ATGCGAACTCAAATTTGTGCGCACCACACCTTTCAGGGGTCCGAGGGGTCGTCCGTCTTC
4861 tatgcaaagcatgcatctcaattagtcagcaaccaggtgtggaaagtccccaggctcccc 4920
ATACGTTTCGTACGTAGAGTTAATCAGTCGTTGGTCCACACCTTTCAGGGGTCCGAGGGG
4921 agcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccatagtcccgcccct 4980
TCGTCCGTCTTCATACGTTTCGTACGTAGAGTTAATCAGTCGTTGGTATCAGGGCGGGGA
SV40 origin(4991,5068)>>>
|
4981 aactccgcccatcccgcccctaactccgcccagttccgcccattctccgccccatggctg 5040
TTGAGGCGGGTAGGGCGGGGATTGAGGCGGGTCAAGGCGGGTAAGAGGCGGGGTACCGAC
SfiI
|
ORF_3 rf(3)(5058,5726)>>>
| |
5041 actaattttttttatttatgcagaggccgaggccgcctcggcctctgagctattccagaa 5100
TGATTAAAAAAAATAAATACGTCTCCGGCTCCGGCGGAGCCGGAGACTCGATAAGGTCTT
puro marker(5127,5726)>>>
|
5101 gtagtgaggaggcttttttggaggccatgaccgagtacaagcccacggtgcgcctcgcca 5160
CATCACTCCTCCGAAAAAACCTCCGGTACTGGCTCATGTTCGGGTGCCACGCGGAGCGGT
5161 cccgcgacgacgtccctcgggccgtacgcaccctcgccgccgcgttcgccgactaccccg 5220
GGGCGCTGCTGCAGGGAGCCCGGCATGCGTGGGAGCGGCGGCGCAAGCGGCTGATGGGGC
5221 ccacgcgccacaccgtggacccggaccgccacatcgagcgggtcaccgagctgcaagaac 5280
GGTGCGCGGTGTGGCACCTGGGCCTGGCGGTGTAGCTCGCCCAGTGGCTCGACGTTCTTG
5281 tcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacggcgccg 5340
AGAAGGAGTGCGCGCAGCCCGAGCTGTAGCCGTTCCACACCCAGCGCCTGCTGCCGCGGC
5341 cggtggcggtctggaccacgccggagagcgtcgaagcgggggcggtgttcgccgagatcg 5400
GCCACCGCCAGACCTGGTGCGGCCTCTCGCAGCTTCGCCCCCGCCACAAGCGGCTCTAGC
5401 gcccgcgcatggccgagttgagcggttcccggctggccgcgcagcaacagatggaagggc 5460
CGGGCGCGTACCGGCTCAACTCGCCAAGGGCCGACCGGCGCGTCGTTGTCTACCTTCCCG
5461 tcctggcgccgcaccggcccaaggagcccgcgtggttcctggccaccgtcggcgtctcgc 5520
AGGACCGCGGCGTGGCCGGGTTCCTCGGGCGCACCAAGGACCGGTGGCAGCCGCAGAGCG
5521 ccgaccaccagggcaagggtctgggcagcgccgtcgtgctccccggagtggaggcggccg 5580
GGCTGGTGGTCCCGTTCCCAGACCCGTCGCGGCAGCACGAGGGGCCTCACCTCCGCCGGC
5581 agcgcgccggggtgcccgccttcctggagacctccgcgccccgcaacctccccttctacg 5640
TCGCGCGGCCCCACGGGCGGAAGGACCTCTGGAGGCGCGGGGCGTTGGAGGGGAAGATGC
5641 agcggctcggcttcaccgtcaccgccgacgtcgaggtgcccgaaggaccgcgcacctggt 5700
TCGCCGAGCCGAAGTGGCAGTGGCGGCTGCAGCTCCACGGGCTTCCTGGCGCGTGGACCA
5701 gcatgacccgcaagcccggtgcctgaacgcgttccggaaatcaacctctggattacaaaa 5760
CGTACTGGGCGTTCGGGCCACGGACTTGCGCAAGGCCTTTAGTTGGAGACCTAATGTTTT
5761 tttgtgaaagattgactggtattcttaactatgttgctccttttacgctatgtggatacg 5820
AAACACTTTCTAACTGACCATAAGAATTGATACAACGAGGAAAATGCGATACACCTATGC
5821 ctgctttaatgcctttgtatcatgctattgcttcccgtatggctttcattttctcctcct 5880
GACGAAATTACGGAAACATAGTACGATAACGAAGGGCATACCGAAAGTAAAAGAGGAGGA
5881 tgtataaatcctggttgctgtctctttatgaggagttgtggcccgttgtcaggcaacgtg 5940
ACATATTTAGGACCAACGACAGAGAAATACTCCTCAACACCGGGCAACAGTCCGTTGCAC
5941 gcgtggtgtgcactgtgtttgctgacgcaacccccactggttggggcattgccaccacct 6000
CGCACCACACGTGACACAAACGACTGCGTTGGGGGTGACCAACCCCGTAACGGTGGTGGA
6001 gtcagctcctttccgggactttcgctttccccctccctattgccacggcggaactcatcg 6060
CAGTCGAGGAAAGGCCCTGAAAGCGAAAGGGGGAGGGATAACGGTGCCGCCTTGAGTAGC
6061 ccgcctgccttgcccgctgctggacaggggctcggctgttgggcactgacaattccgtgg 6120
GGCGGACGGAACGGGCGACGACCTGTCCCCGAGCCGACAACCCGTGACTGTTAAGGCACC
6121 tgttgtcggggaagctgacgtcctttccatggctgctcgcctgtgttgccacctggattc 6180
ACAACAGCCCCTTCGACTGCAGGAAAGGTACCGACGAGCGGACACAACGGTGGACCTAAG
6181 tgcgcgggacgtccttctgctacgtcccttcggccctcaatccagcggaccttccttccc 6240
ACGCGCCCTGCAGGAAGACGATGCAGGGAAGCCGGGAGTTAGGTCGCCTGGAAGGAAGGG
6241 gcggcctgctgccggctctgcggcctcttccgcgtctcgccttcgccctcagacgagtcg 6300
CGCCGGACGACGGCCGAGACGCCGGAGAAGGCGCAGAGCGGAAGCGGGAGTCTGCTCAGC
delta_U3 other(6336,6388)>>>
|
6301 gatctccctttgggccgcctccccgcctgtccggatggaagggctaattcactcccaacg 6360
CTAGAGGGAAACCCGGCGGAGGGGCGGACAGGCCTACCTTCCCGATTAAGTGAGGGTTGC
HIV-1_5_LTR other(6389,6569)>>>
|
6361 aatacaagatctgctttttgcttgtactgggtctctctggttagaccagatctgagcctg 6420
TTATGTTCTAGACGAAAAACGAACATGACCCAGAGAGACCAATCTGGTCTAGACTCGGAC
6421 ggagctctctggctaactagggaacccactgcttaagcctcaataaagcttgccttgagt 6480
CCTCGAGAGACCGATTGATCCCTTGGGTGACGAATTCGGAGTTATTTCGAACGGAACTCA
6481 gcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagacc 6540
CGAAGTTCATCACACACGGGCAGACAACACACTGAGACCATTGATCTCTAGGGAGTCTGG
6541 cttttagtcagtgtggaaaatctctagcagtagtagttcatgtcatcttattattcagta 6600
GAAAATCAGTCACACCTTTTAGAGATCGTCATCATCAAGTACAGTAGAATAATAAGTCAT
6601 tttataacttgcaaagaaatgaatatcagagagtgagaggaacttgtttattgcagctta 6660
AAATATTGAACGTTTCTTTACTTATAGTCTCTCACTCTCCTTGAACAAATAACGTCGAAT
6661 taatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcact 6720
ATTACCAATGTTTATTTCGTTATCGTAGTGTTTAAAGTGTTTATTTCGTAAAAAAAGTGA
6721 gcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctggcatctatg 6780
CGTAAGATCAACACCAAACAGGTTTGAGTAGTTACATAGAATAGTACAGACCGTAGATAC
6781 tcgggtgcggagaaagaggtaatgaaatggcattatgggtattatgggtctgcattaatg 6840
AGCCCACGCCTCTTTCTCCATTACTTTACCGTAATACCCATAATACCCAGACGTAATTAC
6841 aatcggccaacggatgtcggtgtgaaataccgcacagatgcgtaaggagaaaataccgca 6900
TTAGCCGGTTGCCTACAGCCACACTTTATGGCGTGTCTACGCATTCCTCTTTTATGGCGT
6901 tcaggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggc 6960
AGTCCGCGAGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGAGCCAGCAAGCCGACGCCG
6961 gagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacg 7020
CTCGCCATAGTCGAGTGAGTTTCCGCCATTATGCCAATAGGTGTCTTAGTCCCCTATTGC
pBR322 origin(7076,7695)<<<
|
7021 caggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgt 7080
GTCCTTTCTTGTACACTCGTTTTCCGGTCGTTTTCCGGTCCTTGGCATTTTTCCGGCGCA
7081 tgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaa 7140
ACGACCGCAAAAAGGTATCCGAGGCGGGGGGACTGCTCGTAGTGTTTTTAGCTGCGAGTT
7141 gtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagct 7200
CAGTCTCCACCGCTTTGGGCTGTCCTGATATTTCTATGGTCCGCAAAGGGGGACCTTCGA
7201 ccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcc 7260
GGGAGCACGCGAGAGGACAAGGCTGGGACGGCGAATGGCCTATGGACAGGCGGAAAGAGG
7261 cttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtagg 7320
GAAGCCCTTCGCACCGCGAAAGAGTATCGAGTGCGACATCCATAGAGTCAAGCCACATCC
7321 tcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgcct 7380
AGCAAGCGAGGTTCGACCCGACACACGTGCTTGGGGGGCAAGTCGGGCTGGCGACGCGGA
7381 tatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcag 7440
ATAGGCCATTGATAGCAGAACTCAGGTTGGGCCATTCTGTGCTGAATAGCGGTGACCGTC
7441 cagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttga 7500
GTCGGTGACCATTGTCCTAATCGTCTCGCTCCATACATCCGCCACGATGTCTCAAGAACT
7501 agtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctga 7560
TCACCACCGGATTGATGCCGATGTGATCTTCCTGTCATAAACCATAGACGCGAGACGACT
7561 agccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctg 7620
TCGGTCAATGGAAGCCTTTTTCTCAACCATCGAGAACTAGGCCGTTTGTTTGGTGGCGAC
7621 gtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaag 7680
CATCGCCACCAAAAAAACAAACGTTCGTCGTCTAATGCGCGTCTTTTTTTCCTAGAGTTC
7681 aagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaag 7740
TTCTAGGAAACTAGAAAAGATGCCCCAGACTGCGAGTCACCTTGCTTTTGAGTGCAATTC
7741 ggattttggccatgagattatcaaaaaggatcttcacctagatccttttcacgtagaaag 7800
CCTAAAACCGGTACTCTAATAGTTTTTCCTAGAAGTGGATCTAGGAAAAGTGCATCTTTC
7801 ccagtccgcagaaacggtgctgaccccggatgaatgtcagctactgggctatctggacaa 7860
GGTCAGGCGTCTTTGCCACGACTGGGGCCTACTTACAGTCGATGACCCGATAGACCTGTT
7861 gggaaaacgcaagcgcaaagagaaagcaggtagcttgcagtgggcttacatggcgatagc 7920
CCCTTTTGCGTTCGCGTTTCTCTTTCGTCCATCGAACGTCACCCGAATGTACCGCTATCG
NEOKAN prom(7953,8002)>>>
|
7921 tagactgggcggttttatggacagcaagcgaaccggaattgccagctggggcgccctctg 7980
ATCTGACCCGCCAAAATACCTGTCGTTCGCTTGGCCTTAACGGTCGACCCCGCGGGAGAC
7981 gtaaggttgggaagccctgcaaagtaaactggatggctttcttgccgccaaggatctgat 8040
CATTCCAACCCTTCGGGACGTTTCATTTGACCTACCGAAAGAACGGCGGTTCCTAGACTA
NTP_II marker(8094,8882)>>>
|
BclI ORF_4 rf(3)(8091,8885)>>>
| | |
8041 ggcgcaggggatcaagctctgatcaagagacaggatgaggatcgtttcgcatgattgaac 8100
CCGCGTCCCCTAGTTCGAGACTAGTTCTCTGTCCTACTCCTAGCAAAGCGTACTAACTTG
8101 aagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggctatgact 8160
TTCTACCTAACGTGCGTCCAAGAGGCCGGCGAACCCACCTCTCCGATAAGCCGATACTGA
8161 gggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggggc 8220
CCCGTGTTGTCTGTTAGCCGACGAGACTACGGCGGCACAAGGCCGACAGTCGCGTCCCCG
8221 gcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaagacgagg 8280
CGGGCCAAGAAAAACAGTTCTGGCTGGACAGGCCACGGGACTTACTTGACGTTCTGCTCC
8281 cagcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttg 8340
GTCGCGCCGATAGCACCGACCGGTGCTGCCCGCAAGGAACGCGTCGACACGAGCTGCAAC
8341 tcactgaagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcctgt 8400
AGTGACTTCGCCCTTCCCTGACCGACGATAACCCGCTTCACGGCCCCGTCCTAGAGGACA
8401 catctcaccttgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgc 8460
GTAGAGTGGAACGAGGACGGCTCTTTCATAGGTAGTACCGACTACGTTACGCCGCCGACG
8461 atacgcttgatccggctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgag 8520
TATGCGAACTAGGCCGATGGACGGGTAAGCTGGTGGTTCGCTTTGTAGCGTAGCTCGCTC
8521 cacgtactcggatggaagccggtcttgtcgatcaggatgatctggacgaagagcatcagg 8580
GTGCATGAGCCTACCTTCGGCCAGAACAGCTAGTCCTACTAGACCTGCTTCTCGTAGTCC
8581 ggctcgcgccagccgaactgttcgccaggctcaaggcgagcatgcccgacggcgaggatc 8640
CCGAGCGCGGTCGGCTTGACAAGCGGTCCGAGTTCCGCTCGTACGGGCTGCCGCTCCTAG
8641 tcgtcgtgacccatggcgatgcctgcttgccgaatatcatggtggaaaatggccgctttt 8700
AGCAGCACTGGGTACCGCTACGGACGAACGGCTTATAGTACCACCTTTTACCGGCGAAAA
8701 ctggattcatcgactgtggccggctgggtgtggcggaccgctatcaggacatagcgttgg 8760
GACCTAAGTAGCTGACACCGGCCGACCCACACCGCCTGGCGATAGTCCTGTATCGCAACC
8761 ctacccgtgatattgctgaagagcttggcggcgaatgggctgaccgcttcctcgtgcttt 8820
GATGGGCACTATAACGACTTCTCGAACCGCCGCTTACCCGACTGGCGAAGGAGCACGAAA
8821 acggtatcgccgctcccgattcgcagcgcatcgccttctatcgccttcttgacgagttct 8880
TGCCATAGCGGCGAGGGCTAAGCGTCGCGTAGCGGAAGATAGCGGAAGAACTGCTCAAGA
8881 tctgaattttgttaaaatttttgttaaatcagctcattttttaaccaataggccgaaatc 8940
AGACTTAAAACAATTTTAAAAACAATTTAGTCGAGTAAAAAATTGGTTATCCGGCTTTAG
f1 origin(8990,9296)<<<
|
8941 ggcaaaatcccttataaatcaaaagaatagaccgagatagggttgagtgttgttccagtt 9000
CCGTTTTAGGGAATATTTAGTTTTCTTATCTGGCTCTATCCCAACTCACAACAAGGTCAA
9001 tggaacaagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtc 9060
ACCTTGTTCTCAGGTGATAATTTCTTGCACCTGAGGTTGCAGTTTCCCGCTTTTTGGCAG
9061 tatcagggcgatggcccactacgtgaaccatcaccctaatcaagttttttggggtcgagg 9120
ATAGTCCCGCTACCGGGTGATGCACTTGGTAGTGGGATTAGTTCAAAAAACCCCAGCTCC
9121 tgccgtaaagcactaaatcggaaccctaaagggagcccccgatttagagcttgacgggga 9180
ACGGCATTTCGTGATTTAGCCTTGGGATTTCCCTCGGGGGCTAAATCTCGAACTGCCCCT
9181 aagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcg 9240
TTCGGCCGCTTGCACCGCTCTTTCCTTCCCTTCTTTCGCTTTCCTCGCCCGCGATCCCGC
9241 ctggcaagtgtagcggtcacgctgcgcgtaaccaccacacccgccgcgcttaatgcgccg 9300
GACCGTTCACATCGCCAGTGCGACGCGCATTGGTGGTGTGGGCGGCGCGAATTACGCGGC
LITR other(9351,9452)>>>
|
RITR other(9351,9452)<<<
|
9301 ctacagggcgcgtccattcgccattcaggatcgaattaattcttaattaacatcatcaat 9360
GATGTCCCGCGCAGGTAAGCGGTAAGTCCTAGCTTAATTAAGAATTAATTGTAGTAGTTA
9361 aatataccttttttggattgaagccaatatgataatgagggggtggagtttgtgacgtgg 9420
TTATATGGAAAAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACACTGCACC
9421 cgcggggcgtgggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgttgcaag 9480
GCGCCCCGCACCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTACAACGTTC
Encap other(9532,9680)>>>
|
9481 tgtggcggaacacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgccgg 9540
ACACCGCCTTGTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGGCC
9541 tgtacacaggaagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggc 9600
ACATGTGTCCTTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACCCG
9601 gtaaccgagtaagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctg 9660
CATTGGCTCATTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAGAC
9661 aataattttgtgttactcatagcgcgtaatactctcatgagcggatacatatttgaatgt 9720
TTATTAAAACACAATGAGTATCGCGCATTATGAGAGTACTCGCCTATGTATAAACTTACA
9721 atttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgac 9780
TAAATCTTTTTATTTGTTTATCCCCAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTG
9781 gtaactataacggtcctaaggtagcgaaaatgtagtcttatgcaatactcttgtagtctt 9840
CATTGATATTGCCAGGATTCCATCGCTTTTACATCAGAATACGTTATGAGAACATCAGAA
9841 gcaacatggtaacgatgagttagcaacatgccttacaaggagagaaaaagcaccgtgcat 9900
CGTTGTACCATTGCTACTCAATCGTTGTACGGAATGTTCCTCTCTTTTTCGTGGCACGTA
9901 gccgattggtggaagtaaggtggtacgat 9929
CGGCTAACCACCTTCATTCCACCATGCTA
LOCUS dna 9929 bp
FEATURES Location/Qualifiers
Other Gene 109..289
/gene="HIV-1_5_LTR other"
Other Gene 400..444
/gene="HIV-1_psi_pack other"
misc_binding 486..491
/dbxref="REBASE:NruI"
Regulatory_Seq 951..1184
/gene="RRE reg"
ORF 994..1695
/sequence="ORF_1 rf(1)"
ORF 1775..2755
/sequence="ORF_5 rf(5)"
Promoter 1838..3015
/gene="EF1a prom"
misc_binding 2450..2455
/dbxref="REBASE:ApaI"
Other Gene 3041..3165
/gene="attR1 other"
Other Gene 3065..3165
/gene="attR2 other"
misc_binding 3166..3173
/dbxref="REBASE:NotI"
Marker 3274..3933
/gene="CAT marker"
ORF 3274..3933
/sequence="ORF_2 rf(1)"
misc_binding 3487..3492
/dbxref="REBASE:EcoRI"
Other Gene 4275..4580
/gene="ccdB other"
misc_binding 4356..4361
/dbxref="REBASE:SmaI"
misc_binding 4356..4361
/dbxref="REBASE:XmaI"
Other Gene 4621..4740
/gene="attR1 other"
Other Gene 4621..4721
/gene="attR2 other"
misc_binding 4762..4767
/dbxref="REBASE:XbaI"
Promoter 4824..5092
/gene="SV40 prom"
Rep_Origin 4991..5068
/gene="SV40 origin"
ORF 5058..5726
/sequence="ORF_3 rf(3)"
misc_binding 5071..5083
/dbxref="REBASE:SfiI"
Marker 5127..5726
/gene="puro marker"
Other Gene 6336..6388
/gene="delta_U3 other"
Other Gene 6389..6569
/gene="HIV-1_5_LTR other"
Rep_Origin 7076..7695
/gene="pBR322 origin"
Promoter 7953..8002
/gene="NEOKAN prom"
misc_binding 8060..8065
/dbxref="REBASE:BclI"
ORF 8091..8885
/sequence="ORF_4 rf(3)"
Marker 8094..8882
/gene="NTP_II marker"
Rep_Origin 8990..9296
/gene="f1 origin"
Other Gene 9351..9452
/gene="RITR other"
Other Gene 9351..9452
/gene="LITR other"
Other Gene 9532..9680
/gene="Encap other"
BASE COUNT 2442 a 2373 c 2742 g 2372 t 0 others