pLenti-III-EF1alpha
| Vector Name | pLenti-III-EF1alpha |
| VectorType | Lentivirus |
| Antibiotic Information | Puromycin |
| Sequencing Primers | EF1a sequencing primer 5'--TCA GAC AGT GGT TCA AAG--3' SV40 reverse sequencing primer 5`---TAG TCA GCC ATG GGG CGG AGA ---3` |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
61 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
121 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
181 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
241 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
301 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
361 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
421 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
481 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
541 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
601 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
661 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
721 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
781 agaccaccgc acagcaagcc cgctgatctt cagacctgga ggaggagata tgagggacat
841 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
901 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
961 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
1021 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
1081 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
1141 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
1201 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
1261 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
1321 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
1381 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
1441 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
1501 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
1561 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
1621 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgaaagctt
1681 gggattcgaa tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
1741 agacataata gcaacagaca tacaaactaa agaactacaa aaacaaatta caaaaattca
1801 aaattttcgg gtttttcgaa cctagggagt gggaattggc tccggtgccc gtcagtgggc
1861 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
1921 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
1981 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
2041 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
2101 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccactggct gcagtacgtg
2161 attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa
2221 ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg
2281 cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa
2341 aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc
2401 caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg
2461 tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg
2521 gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc
2581 ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg
2641 cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg
2701 ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac
2761 tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg
2821 tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg
2881 gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt
2941 gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca
3001 tttcaggtgt cgtgaggaat ttcgactgct agctggtacc tgaattgagt acttacgtag
3061 gtaccccagt gtggtggcct gcaggtgaat tcactagtac cggtaggcct gtcgacgata
3121 tcgggcccgc ggccgctgga tcctctagac tgcagctcga gtacccatac gacgtcccag
3181 actacgcttg agtttaaaca cgcgtggtgt ggaaagtccc caggctcccc agcaggcaga
3241 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
3301 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
3361 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
3421 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
3481 aagtagtgag gaggcttttt tggaggccat gaccgagtac aagcccacgg tgcgcctcgc
3541 cacccgcgac gacgtccctc gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
3601 cgccacgcgc cacaccgtgg acccggaccg ccacatcgag cgggtcaccg agctgcaaga
3661 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc
3721 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat
3781 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg
3841 gctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc
3901 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
3961 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta
4021 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg
4081 gtgcatgacc cgcaagcccg gtgcctgaac gcgttccgga aatcaacctc tggattacaa
4141 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
4201 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
4261 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
4321 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
4381 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
4441 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
4501 ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat
4561 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
4621 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctc gccttcgccc tcagacgagt
4681 cggatctccc tttgggccgc ctccccgcct gtccggatgg aagggctaat tcactcccaa
4741 cgaatacaag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
4801 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
4861 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
4921 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag
4981 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct
5041 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
5101 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggcatcta
5161 tgtcgggtgc ggagaaagag gtaatgaaat ggcattatgg gtattatggg tctgcattaa
5221 tgaatcggcc aacggatgtc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
5281 catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
5341 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
5401 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
5461 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
5521 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
5581 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
5641 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
5701 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
5761 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
5821 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
5881 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
5941 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
6001 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
6061 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
6121 agggattttg gccatgagat tatcaaaaag gatcttcacc tagatccttt tcacgtagaa
6181 agccagtccg cagaaacggt gctgaccccg gatgaatgtc agctactggg ctatctggac
6241 aagggaaaac gcaagcgcaa agagaaagca ggtagcttgc agtgggctta catggcgata
6301 gctagactgg gcggttttat ggacagcaag cgaaccggaa ttgccagctg gggcgccctc
6361 tggtaaggtt gggaagccct gcaaagtaaa ctggatggct ttcttgccgc caaggatctg
6421 atggcgcagg ggatcaagct ctgatcaaga gacaggatga ggatcgtttc gcatgattga
6481 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
6541 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
6601 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga
6661 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
6721 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
6781 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
6841 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
6901 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
6961 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga
7021 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
7081 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
7141 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
7201 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
7261 cttctgaatt ttgttaaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
7321 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
7381 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
7441 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
7501 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
7561 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
7621 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
7681 cgctacaggg cgcgtccatt cgccattcag gatcgaatta attcttaatt aacatcatca
7741 ataatatacc ttttttggat tgaagccaat atgataatga gggggtggag tttgtgacgt
7801 ggcgcggggc gtgggaacgg ggcgggtgac gtagtagtgt ggcggaagtg tgatgttgca
7861 agtgtggcgg aacacatgta agcgacggat gtggcaaaag tgacgttttt ggtgtgcgcc
7921 ggtgtacaca ggaagtgaca attttcgcgc ggttttaggc ggatgttgta gtaaatttgg
7981 gcgtaaccga gtaagatttg gccattttcg cgggaaaact gaataagagg aagtgaaatc
8041 tgaataattt tgtgttactc atagcgcgta atactctcat gagcggatac atatttgaat
8101 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
8161 acgtaactat aacggtccta aggtagcgaa aatgtagtct tatgcaatac tcttgtagtc
8221 ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc
8281 atgccgattg gtggaagtaa ggtggtacga t
//
LOCUS dna 8311 bp
FEATURES Location/Qualifiers
Other Gene 109..289
/gene="HIV-1_5_LTR other"
Other Gene 400..444
/gene="HIV-1_psi_pack other"
misc_binding 486..491
/dbxref="REBASE:NruI"
Regulatory_Seq 951..1184
/gene="RRE reg"
ORF 994..1695
/sequence="ORF_1 rf(1)"
ORF 1775..2755
/sequence="ORF_4 rf(4)"
Promoter 1838..3015
/gene="EF1a prom"
misc_binding 3087..3092
/dbxref="REBASE:EcoRI"
misc_binding 3111..3116
/dbxref="REBASE:HincII"
misc_binding 3117..3122
/dbxref="REBASE:EcoRV"
misc_binding 3129..3136
/dbxref="REBASE:NotI"
misc_binding 3138..3143
/dbxref="REBASE:BamHI"
misc_binding 3144..3149
/dbxref="REBASE:XbaI"
Promoter 3206..3474
/gene="SV40 prom"
Rep_Origin 3373..3450
/gene="SV40 origin"
ORF 3440..4108
/sequence="ORF_2 rf(2)"
misc_binding 3453..3465
/dbxref="REBASE:SfiI"
Marker 3509..4108
/gene="puro marker"
Other Gene 4718..4770
/gene="delta_U3 other"
Other Gene 4771..4951
/gene="HIV-1_5_LTR other"
Rep_Origin 5458..6077
/gene="pBR322 origin"
Promoter 6335..6384
/gene="NEOKAN prom"
misc_binding 6442..6447
/dbxref="REBASE:BclI"
ORF 6473..7267
/sequence="ORF_3 rf(2)"
Marker 6476..7264
/gene="NTP_II marker"
Rep_Origin 7372..7678
/gene="f1 origin"
Other Gene 7733..7834
/gene="RITR other"
Other Gene 7733..7834
/gene="LITR other"
Other Gene 7914..8062
/gene="Encap other"
BASE COUNT 1974 a 2052 c 2371 g 1914 t 0 others
ORIGIN
1 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
61 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
121 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
181 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
241 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
301 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
361 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
421 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
481 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
541 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
601 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
661 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
721 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
781 agaccaccgc acagcaagcc cgctgatctt cagacctgga ggaggagata tgagggacat
841 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
901 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
961 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
1021 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
1081 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
1141 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
1201 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
1261 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
1321 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
1381 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
1441 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
1501 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
1561 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
1621 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgaaagctt
1681 gggattcgaa tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
1741 agacataata gcaacagaca tacaaactaa agaactacaa aaacaaatta caaaaattca
1801 aaattttcgg gtttttcgaa cctagggagt gggaattggc tccggtgccc gtcagtgggc
1861 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
1921 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
1981 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
2041 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
2101 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccactggct gcagtacgtg
2161 attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa
2221 ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg
2281 cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa
2341 aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc
2401 caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg
2461 tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg
2521 gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc
2581 ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg
2641 cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg
2701 ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac
2761 tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg
2821 tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg
2881 gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt
2941 gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca
3001 tttcaggtgt cgtgaggaat ttcgactgct agctggtacc tgaattgagt acttacgtag
3061 gtaccccagt gtggtggcct gcaggtgaat tcactagtac cggtaggcct gtcgacgata
3121 tcgggcccgc ggccgctgga tcctctagac tgcagctcga gtacccatac gacgtcccag
3181 actacgcttg agtttaaaca cgcgtggtgt ggaaagtccc caggctcccc agcaggcaga
3241 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
3301 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
3361 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
3421 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
3481 aagtagtgag gaggcttttt tggaggccat gaccgagtac aagcccacgg tgcgcctcgc
3541 cacccgcgac gacgtccctc gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
3601 cgccacgcgc cacaccgtgg acccggaccg ccacatcgag cgggtcaccg agctgcaaga
3661 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc
3721 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat
3781 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg
3841 gctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc
3901 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
3961 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta
4021 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg
4081 gtgcatgacc cgcaagcccg gtgcctgaac gcgttccgga aatcaacctc tggattacaa
4141 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
4201 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
4261 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
4321 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
4381 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
4441 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
4501 ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat
4561 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
4621 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctc gccttcgccc tcagacgagt
4681 cggatctccc tttgggccgc ctccccgcct gtccggatgg aagggctaat tcactcccaa
4741 cgaatacaag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
4801 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
4861 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
4921 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag
4981 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct
5041 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
5101 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggcatcta
5161 tgtcgggtgc ggagaaagag gtaatgaaat ggcattatgg gtattatggg tctgcattaa
5221 tgaatcggcc aacggatgtc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
5281 catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
5341 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
5401 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
5461 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
5521 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
5581 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
5641 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
5701 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
5761 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
5821 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
5881 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
5941 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
6001 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
6061 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
6121 agggattttg gccatgagat tatcaaaaag gatcttcacc tagatccttt tcacgtagaa
6181 agccagtccg cagaaacggt gctgaccccg gatgaatgtc agctactggg ctatctggac
6241 aagggaaaac gcaagcgcaa agagaaagca ggtagcttgc agtgggctta catggcgata
6301 gctagactgg gcggttttat ggacagcaag cgaaccggaa ttgccagctg gggcgccctc
6361 tggtaaggtt gggaagccct gcaaagtaaa ctggatggct ttcttgccgc caaggatctg
6421 atggcgcagg ggatcaagct ctgatcaaga gacaggatga ggatcgtttc gcatgattga
6481 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
6541 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
6601 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga
6661 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
6721 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
6781 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
6841 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
6901 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
6961 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga
7021 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
7081 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
7141 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
7201 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
7261 cttctgaatt ttgttaaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
7321 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
7381 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
7441 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
7501 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
7561 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
7621 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
7681 cgctacaggg cgcgtccatt cgccattcag gatcgaatta attcttaatt aacatcatca
7741 ataatatacc ttttttggat tgaagccaat atgataatga gggggtggag tttgtgacgt
7801 ggcgcggggc gtgggaacgg ggcgggtgac gtagtagtgt ggcggaagtg tgatgttgca
7861 agtgtggcgg aacacatgta agcgacggat gtggcaaaag tgacgttttt ggtgtgcgcc
7921 ggtgtacaca ggaagtgaca attttcgcgc ggttttaggc ggatgttgta gtaaatttgg
7981 gcgtaaccga gtaagatttg gccattttcg cgggaaaact gaataagagg aagtgaaatc
8041 tgaataattt tgtgttactc atagcgcgta atactctcat gagcggatac atatttgaat
8101 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
8161 acgtaactat aacggtccta aggtagcgaa aatgtagtct tatgcaatac tcttgtagtc
8221 ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc
8281 atgccgattg gtggaagtaa ggtggtacga t
//
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 cgtgccttattaggaaggcaacagacgggtctgacatggattggacgaaccactgaattg 60
GCACGGAATAATCCTTCCGTTGTCTGCCCAGACTGTACCTAACCTGCTTGGTGACTTAAC
HIV-1_5_LTR other(109,289)>>>
|
61 ccgcattgcagagatattgtatttaagtgcctagctcgatacataaacgggtctctctgg 120
GGCGTAACGTCTCTATAACATAAATTCACGGATCGAGCTATGTATTTGCCCAGAGAGACC
121 ttagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcct 180
AATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAATTCGGA
181 caataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggt 240
GTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTGAGACCA
241 aactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggcgcccga 300
TTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCACCGCGGGCT
301 acagggacttgaaagcgaaagggaaaccagaggagctctctcgacgcaggactcggcttg 360
TGTCCCTGAACTTTCGCTTTCCCTTTGGTCTCCTCGAGAGAGCTGCGTCCTGAGCCGAAC
HIV-1_psi_pack other(400,444)>>>
|
361 ctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaattttga 420
GACTTCGCGCGTGCCGTTCTCCGCTCCCCGCCGCTGACCACTCATGCGGTTTTTAAAACT
421 ctagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcgggggagaa 480
GATCGCCTCCGATCTTCCTCTCTCTACCCACGCTCTCGCAGTCATAATTCGCCCCCTCTT
NruI
|
481 ttagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatataaattaa 540
AATCTAGCGCTACCCTTTTTTAAGCCAATTCCGGTCCCCCTTTCTTTTTTATATTTAATT
541 aacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcctgttag 600
TTGTATATCATACCCGTTCGTCCCTCGATCTTGCTAAGCGTCAATTAGGACCGGACAATC
601 aaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcagacaggat 660
TTTGTAGTCTTCCGACATCTGTTTATGACCCTGTCGATGTTGGTAGGGAAGTCTGTCCTA
661 cagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatcaaagga 720
GTCTTCTTGAATCTAGTAATATATTATGTCATCGTTGGGAGATAACACACGTAGTTTCCT
721 tagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaacaaaagta 780
ATCTCTATTTTCTGTGGTTCCTTCGAAATCTGTTCTATCTCCTTCTCGTTTTGTTTTCAT
781 agaccaccgcacagcaagcccgctgatcttcagacctggaggaggagatatgagggacat 840
TCTGGTGGCGTGTCGTTCGGGCGACTAGAAGTCTGGACCTCCTCCTCTATACTCCCTGTA
841 tggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagtagcaccc 900
ACCTCTTCACTTAATATATTTATATTTCATCATTTTTAACTTGGTAATCCTCATCGTGGG
RRE reg(951,1184)>>>
|
901 accaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaataggagctttg 960
TGGTTCCGTTTCTCTTCTCACCACGTCTCTCTTTTTTCTCGTCACCCTTATCCTCGAAAC
ORF_1 rf(1)(994,1695)>>>
|
961 ttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacgctgacg 1020
AAGGAACCCAAGAACCCTCGTCGTCCTTCGTGATACCCGCGTCGCAGTTACTGCGACTGC
1021 gtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctgagggct 1080
CATGTCCGGTCTGTTAATAACAGACCATATCACGTCGTCGTCTTGTTAAACGACTCCCGA
1081 attgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctccaggca 1140
TAACTCCGCGTTGTCGTAGACAACGTTGAGTGTCAGACCCCGTAGTTCGTCGAGGTCCGT
1141 agaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttggggttgc 1200
TCTTAGGACCGACACCTTTCTATGGATTTCCTAGTTGTCGAGGACCCCTAAACCCCAACG
1201 tctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaataaatct 1260
AGACCTTTTGAGTAAACGTGGTGACGACACGGAACCTTACGATCAACCTCATTATTTAGA
1261 ctggaacagatttggaatcacacgacctggatggagtgggacagagaaattaacaattac 1320
GACCTTGTCTAAACCTTAGTGTGCTGGACCTACCTCACCCTGTCTCTTTAATTGTTAATG
1321 acaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaatgaacaa 1380
TGTTCGAATTATGTGAGGAATTAACTTCTTAGCGTTTTGGTCGTTCTTTTCTTACTTGTT
1381 gaattattggaattagataaatgggcaagtttgtggaattggtttaacataacaaattgg 1440
CTTAATAACCTTAATCTATTTACCCGTTCAAACACCTTAACCAAATTGTATTGTTTAACC
1441 ctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaagaatagtt 1500
GACACCATATATTTTAATAAGTATTACTATCATCCTCCGAACCATCCAAATTCTTATCAA
1501 tttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcgtttcag 1560
AAACGACATGAAAGATATCACTTATCTCAATCCGTCCCTATAAGTGGTAATAGCAAAGTC
1561 acccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaaggtgga 1620
TGGGTGGAGGGTTGGGGCTCCCCTGGGCTGTCCGGGCTTCCTTATCTTCTTCTTCCACCT
1621 gagagagacagagacagatccattcgattagtgaacggatctcgacggtatcgaaagctt 1680
CTCTCTCTGTCTCTGTCTAGGTAAGCTAATCACTTGCCTAGAGCTGCCATAGCTTTCGAA
1681 gggattcgaatttaaaagaaaaggggggattggggggtacagtgcaggggaaagaatagt 1740
CCCTAAGCTTAAATTTTCTTTTCCCCCCTAACCCCCCATGTCACGTCCCCTTTCTTATCA
ORF_4 rf(4)(1775,2755)<<<
|
1741 agacataatagcaacagacatacaaactaaagaactacaaaaacaaattacaaaaattca 1800
TCTGTATTATCGTTGTCTGTATGTTTGATTTCTTGATGTTTTTGTTTAATGTTTTTAAGT
EF1a prom(1838,3015)>>>
|
1801 aaattttcgggtttttcgaacctagggagtgggaattggctccggtgcccgtcagtgggc 1860
TTTAAAAGCCCAAAAAGCTTGGATCCCTCACCCTTAACCGAGGCCACGGGCAGTCACCCG
1861 agagcgcacatcgcccacagtccccgagaagttggggggaggggtcggcaattgaaccgg 1920
TCTCGCGTGTAGCGGGTGTCAGGGGCTCTTCAACCCCCCTCCCCAGCCGTTAACTTGGCC
1921 tgcctagagaaggtggcgcggggtaaactgggaaagtgatgtcgtgtactggctccgcct 1980
ACGGATCTCTTCCACCGCGCCCCATTTGACCCTTTCACTACAGCACATGACCGAGGCGGA
1981 ttttcccgagggtgggggagaaccgtatataagtgcagtagtcgccgtgaacgttctttt 2040
AAAAGGGCTCCCACCCCCTCTTGGCATATATTCACGTCATCAGCGGCACTTGCAAGAAAA
2041 tcgcaacgggtttgccgccagaacacaggtaagtgccgtgtgtggttcccgcgggcctgg 2100
AGCGTTGCCCAAACGGCGGTCTTGTGTCCATTCACGGCACACACCAAGGGCGCCCGGACC
2101 cctctttacgggttatggcccttgcgtgccttgaattacttccactggctgcagtacgtg 2160
GGAGAAATGCCCAATACCGGGAACGCACGGAACTTAATGAAGGTGACCGACGTCATGCAC
2161 attcttgatcccgagcttcgggttggaagtgggtgggagagttcgaggccttgcgcttaa 2220
TAAGAACTAGGGCTCGAAGCCCAACCTTCACCCACCCTCTCAAGCTCCGGAACGCGAATT
2221 ggagccccttcgcctcgtgcttgagttgaggcctggcctgggcgctggggccgccgcgtg 2280
CCTCGGGGAAGCGGAGCACGAACTCAACTCCGGACCGGACCCGCGACCCCGGCGGCGCAC
2281 cgaatctggtggcaccttcgcgcctgtctcgctgctttcgataagtctctagccatttaa 2340
GCTTAGACCACCGTGGAAGCGCGGACAGAGCGACGAAAGCTATTCAGAGATCGGTAAATT
2341 aatttttgatgacctgctgcgacgctttttttctggcaagatagtcttgtaaatgcgggc 2400
TTAAAAACTACTGGACGACGCTGCGAAAAAAAGACCGTTCTATCAGAACATTTACGCCCG
2401 caagatctgcacactggtatttcggtttttggggccgcgggcggcgacggggcccgtgcg 2460
GTTCTAGACGTGTGACCATAAAGCCAAAAACCCCGGCGCCCGCCGCTGCCCCGGGCACGC
2461 tcccagcgcacatgttcggcgaggcggggcctgcgagcgcggccaccgagaatcggacgg 2520
AGGGTCGCGTGTACAAGCCGCTCCGCCCCGGACGCTCGCGCCGGTGGCTCTTAGCCTGCC
2521 gggtagtctcaagctggccggcctgctctggtgcctggcctcgcgccgccgtgtatcgcc 2580
CCCATCAGAGTTCGACCGGCCGGACGAGACCACGGACCGGAGCGCGGCGGCACATAGCGG
2581 ccgccctgggcggcaaggctggcccggtcggcaccagttgcgtgagcggaaagatggccg 2640
GGCGGGACCCGCCGTTCCGACCGGGCCAGCCGTGGTCAACGCACTCGCCTTTCTACCGGC
2641 cttcccggccctgctgcagggagctcaaaatggaggacgcggcgctcgggagagcgggcg 2700
GAAGGGCCGGGACGACGTCCCTCGAGTTTTACCTCCTGCGCCGCGAGCCCTCTCGCCCGC
2701 ggtgagtcacccacacaaaggaaaagggcctttccgtcctcagccgtcgcttcatgtgac 2760
CCACTCAGTGGGTGTGTTTCCTTTTCCCGGAAAGGCAGGAGTCGGCAGCGAAGTACACTG
2761 tccacggagtaccgggcgccgtccaggcacctcgattagttctcgagcttttggagtacg 2820
AGGTGCCTCATGGCCCGCGGCAGGTCCGTGGAGCTAATCAAGAGCTCGAAAACCTCATGC
2821 tcgtctttaggttggggggaggggttttatgcgatggagtttccccacactgagtgggtg 2880
AGCAGAAATCCAACCCCCCTCCCCAAAATACGCTACCTCAAAGGGGTGTGACTCACCCAC
2881 gagactgaagttaggccagcttggcacttgatgtaattctccttggaatttgcccttttt 2940
CTCTGACTTCAATCCGGTCGAACCGTGAACTACATTAAGAGGAACCTTAAACGGGAAAAA
2941 gagtttggatcttggttcattctcaagcctcagacagtggttcaaagtttttttcttcca 3000
CTCAAACCTAGAACCAAGTAAGAGTTCGGAGTCTGTCACCAAGTTTCAAAAAAAGAAGGT
3001 tttcaggtgtcgtgaggaatttcgactgctagctggtacctgaattgagtacttacgtag 3060
AAAGTCCACAGCACTCCTTAAAGCTGACGATCGACCATGGACTTAACTCATGAATGCATC
EcoRI HincII
| |
3061 gtaccccagtgtggtggcctgcaggtgaattcactagtaccggtaggcctgtcgacgata 3120
CATGGGGTCACACCACCGGACGTCCACTTAAGTGATCATGGCCATCCGGACAGCTGCTAT
EcoRV NotI BamHI XbaI
| | | |
3121 tcgggcccgcggccgctggatcctctagactgcagctcgagtacccatacgacgtcccag 3180
AGCCCGGGCGCCGGCGACCTAGGAGATCTGACGTCGAGCTCATGGGTATGCTGCAGGGTC
SV40 prom(3206,3474)>>>
|
3181 actacgcttgagtttaaacacgcgtggtgtggaaagtccccaggctccccagcaggcaga 3240
TGATGCGAACTCAAATTTGTGCGCACCACACCTTTCAGGGGTCCGAGGGGTCGTCCGTCT
3241 agtatgcaaagcatgcatctcaattagtcagcaaccaggtgtggaaagtccccaggctcc 3300
TCATACGTTTCGTACGTAGAGTTAATCAGTCGTTGGTCCACACCTTTCAGGGGTCCGAGG
3301 ccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccatagtcccgccc 3360
GGTCGTCCGTCTTCATACGTTTCGTACGTAGAGTTAATCAGTCGTTGGTATCAGGGCGGG
SV40 origin(3373,3450)>>>
|
3361 ctaactccgcccatcccgcccctaactccgcccagttccgcccattctccgccccatggc 3420
GATTGAGGCGGGTAGGGCGGGGATTGAGGCGGGTCAAGGCGGGTAAGAGGCGGGGTACCG
SfiI
|
ORF_2 rf(2)(3440,4108)>>>
| |
3421 tgactaattttttttatttatgcagaggccgaggccgcctcggcctctgagctattccag 3480
ACTGATTAAAAAAAATAAATACGTCTCCGGCTCCGGCGGAGCCGGAGACTCGATAAGGTC
puro marker(3509,4108)>>>
|
3481 aagtagtgaggaggcttttttggaggccatgaccgagtacaagcccacggtgcgcctcgc 3540
TTCATCACTCCTCCGAAAAAACCTCCGGTACTGGCTCATGTTCGGGTGCCACGCGGAGCG
3541 cacccgcgacgacgtccctcgggccgtacgcaccctcgccgccgcgttcgccgactaccc 3600
GTGGGCGCTGCTGCAGGGAGCCCGGCATGCGTGGGAGCGGCGGCGCAAGCGGCTGATGGG
3601 cgccacgcgccacaccgtggacccggaccgccacatcgagcgggtcaccgagctgcaaga 3660
GCGGTGCGCGGTGTGGCACCTGGGCCTGGCGGTGTAGCTCGCCCAGTGGCTCGACGTTCT
3661 actcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacggcgc 3720
TGAGAAGGAGTGCGCGCAGCCCGAGCTGTAGCCGTTCCACACCCAGCGCCTGCTGCCGCG
3721 cgcggtggcggtctggaccacgccggagagcgtcgaagcgggggcggtgttcgccgagat 3780
GCGCCACCGCCAGACCTGGTGCGGCCTCTCGCAGCTTCGCCCCCGCCACAAGCGGCTCTA
3781 cggcccgcgcatggccgagttgagcggttcccggctggccgcgcagcaacagatggaagg 3840
GCCGGGCGCGTACCGGCTCAACTCGCCAAGGGCCGACCGGCGCGTCGTTGTCTACCTTCC
3841 gctcctggcgccgcaccggcccaaggagcccgcgtggttcctggccaccgtcggcgtctc 3900
CGAGGACCGCGGCGTGGCCGGGTTCCTCGGGCGCACCAAGGACCGGTGGCAGCCGCAGAG
3901 gcccgaccaccagggcaagggtctgggcagcgccgtcgtgctccccggagtggaggcggc 3960
CGGGCTGGTGGTCCCGTTCCCAGACCCGTCGCGGCAGCACGAGGGGCCTCACCTCCGCCG
3961 cgagcgcgccggggtgcccgccttcctggagacctccgcgccccgcaacctccccttcta 4020
GCTCGCGCGGCCCCACGGGCGGAAGGACCTCTGGAGGCGCGGGGCGTTGGAGGGGAAGAT
4021 cgagcggctcggcttcaccgtcaccgccgacgtcgaggtgcccgaaggaccgcgcacctg 4080
GCTCGCCGAGCCGAAGTGGCAGTGGCGGCTGCAGCTCCACGGGCTTCCTGGCGCGTGGAC
4081 gtgcatgacccgcaagcccggtgcctgaacgcgttccggaaatcaacctctggattacaa 4140
CACGTACTGGGCGTTCGGGCCACGGACTTGCGCAAGGCCTTTAGTTGGAGACCTAATGTT
4141 aatttgtgaaagattgactggtattcttaactatgttgctccttttacgctatgtggata 4200
TTAAACACTTTCTAACTGACCATAAGAATTGATACAACGAGGAAAATGCGATACACCTAT
4201 cgctgctttaatgcctttgtatcatgctattgcttcccgtatggctttcattttctcctc 4260
GCGACGAAATTACGGAAACATAGTACGATAACGAAGGGCATACCGAAAGTAAAAGAGGAG
4261 cttgtataaatcctggttgctgtctctttatgaggagttgtggcccgttgtcaggcaacg 4320
GAACATATTTAGGACCAACGACAGAGAAATACTCCTCAACACCGGGCAACAGTCCGTTGC
4321 tggcgtggtgtgcactgtgtttgctgacgcaacccccactggttggggcattgccaccac 4380
ACCGCACCACACGTGACACAAACGACTGCGTTGGGGGTGACCAACCCCGTAACGGTGGTG
4381 ctgtcagctcctttccgggactttcgctttccccctccctattgccacggcggaactcat 4440
GACAGTCGAGGAAAGGCCCTGAAAGCGAAAGGGGGAGGGATAACGGTGCCGCCTTGAGTA
4441 cgccgcctgccttgcccgctgctggacaggggctcggctgttgggcactgacaattccgt 4500
GCGGCGGACGGAACGGGCGACGACCTGTCCCCGAGCCGACAACCCGTGACTGTTAAGGCA
4501 ggtgttgtcggggaagctgacgtcctttccatggctgctcgcctgtgttgccacctggat 4560
CCACAACAGCCCCTTCGACTGCAGGAAAGGTACCGACGAGCGGACACAACGGTGGACCTA
4561 tctgcgcgggacgtccttctgctacgtcccttcggccctcaatccagcggaccttccttc 4620
AGACGCGCCCTGCAGGAAGACGATGCAGGGAAGCCGGGAGTTAGGTCGCCTGGAAGGAAG
4621 ccgcggcctgctgccggctctgcggcctcttccgcgtctcgccttcgccctcagacgagt 4680
GGCGCCGGACGACGGCCGAGACGCCGGAGAAGGCGCAGAGCGGAAGCGGGAGTCTGCTCA
delta_U3 other(4718,4770)>>>
|
4681 cggatctccctttgggccgcctccccgcctgtccggatggaagggctaattcactcccaa 4740
GCCTAGAGGGAAACCCGGCGGAGGGGCGGACAGGCCTACCTTCCCGATTAAGTGAGGGTT
HIV-1_5_LTR other(4771,4951)>>>
|
4741 cgaatacaagatctgctttttgcttgtactgggtctctctggttagaccagatctgagcc 4800
GCTTATGTTCTAGACGAAAAACGAACATGACCCAGAGAGACCAATCTGGTCTAGACTCGG
4801 tgggagctctctggctaactagggaacccactgcttaagcctcaataaagcttgccttga 4860
ACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAATTCGGAGTTATTTCGAACGGAACT
4861 gtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcaga 4920
CACGAAGTTCATCACACACGGGCAGACAACACACTGAGACCATTGATCTCTAGGGAGTCT
4921 cccttttagtcagtgtggaaaatctctagcagtagtagttcatgtcatcttattattcag 4980
GGGAAAATCAGTCACACCTTTTAGAGATCGTCATCATCAAGTACAGTAGAATAATAAGTC
4981 tatttataacttgcaaagaaatgaatatcagagagtgagaggaacttgtttattgcagct 5040
ATAAATATTGAACGTTTCTTTACTTATAGTCTCTCACTCTCCTTGAACAAATAACGTCGA
5041 tataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttca 5100
ATATTACCAATGTTTATTTCGTTATCGTAGTGTTTAAAGTGTTTATTTCGTAAAAAAAGT
5101 ctgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctggcatcta 5160
GACGTAAGATCAACACCAAACAGGTTTGAGTAGTTACATAGAATAGTACAGACCGTAGAT
5161 tgtcgggtgcggagaaagaggtaatgaaatggcattatgggtattatgggtctgcattaa 5220
ACAGCCCACGCCTCTTTCTCCATTACTTTACCGTAATACCCATAATACCCAGACGTAATT
5221 tgaatcggccaacggatgtcggtgtgaaataccgcacagatgcgtaaggagaaaataccg 5280
ACTTAGCCGGTTGCCTACAGCCACACTTTATGGCGTGTCTACGCATTCCTCTTTTATGGC
5281 catcaggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcg 5340
GTAGTCCGCGAGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGAGCCAGCAAGCCGACGC
5341 gcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataa 5400
CGCTCGCCATAGTCGAGTGAGTTTCCGCCATTATGCCAATAGGTGTCTTAGTCCCCTATT
pBR322 origin(5458,6077)<<<
|
5401 cgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgc 5460
GCGTCCTTTCTTGTACACTCGTTTTCCGGTCGTTTTCCGGTCCTTGGCATTTTTCCGGCG
5461 gttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctc 5520
CAACGACCGCAAAAAGGTATCCGAGGCGGGGGGACTGCTCGTAGTGTTTTTAGCTGCGAG
5521 aagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaag 5580
TTCAGTCTCCACCGCTTTGGGCTGTCCTGATATTTCTATGGTCCGCAAAGGGGGACCTTC
5581 ctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttct 5640
GAGGGAGCACGCGAGAGGACAAGGCTGGGACGGCGAATGGCCTATGGACAGGCGGAAAGA
5641 cccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgta 5700
GGGAAGCCCTTCGCACCGCGAAAGAGTATCGAGTGCGACATCCATAGAGTCAAGCCACAT
5701 ggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgc 5760
CCAGCAAGCGAGGTTCGACCCGACACACGTGCTTGGGGGGCAAGTCGGGCTGGCGACGCG
5761 cttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggc 5820
GAATAGGCCATTGATAGCAGAACTCAGGTTGGGCCATTCTGTGCTGAATAGCGGTGACCG
5821 agcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttctt 5880
TCGTCGGTGACCATTGTCCTAATCGTCTCGCTCCATACATCCGCCACGATGTCTCAAGAA
5881 gaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgct 5940
CTTCACCACCGGATTGATGCCGATGTGATCTTCCTGTCATAAACCATAGACGCGAGACGA
5941 gaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgc 6000
CTTCGGTCAATGGAAGCCTTTTTCTCAACCATCGAGAACTAGGCCGTTTGTTTGGTGGCG
6001 tggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctca 6060
ACCATCGCCACCAAAAAAACAAACGTTCGTCGTCTAATGCGCGTCTTTTTTTCCTAGAGT
6061 agaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgtta 6120
TCTTCTAGGAAACTAGAAAAGATGCCCCAGACTGCGAGTCACCTTGCTTTTGAGTGCAAT
6121 agggattttggccatgagattatcaaaaaggatcttcacctagatccttttcacgtagaa 6180
TCCCTAAAACCGGTACTCTAATAGTTTTTCCTAGAAGTGGATCTAGGAAAAGTGCATCTT
6181 agccagtccgcagaaacggtgctgaccccggatgaatgtcagctactgggctatctggac 6240
TCGGTCAGGCGTCTTTGCCACGACTGGGGCCTACTTACAGTCGATGACCCGATAGACCTG
6241 aagggaaaacgcaagcgcaaagagaaagcaggtagcttgcagtgggcttacatggcgata 6300
TTCCCTTTTGCGTTCGCGTTTCTCTTTCGTCCATCGAACGTCACCCGAATGTACCGCTAT
NEOKAN prom(6335,6384)>>>
|
6301 gctagactgggcggttttatggacagcaagcgaaccggaattgccagctggggcgccctc 6360
CGATCTGACCCGCCAAAATACCTGTCGTTCGCTTGGCCTTAACGGTCGACCCCGCGGGAG
6361 tggtaaggttgggaagccctgcaaagtaaactggatggctttcttgccgccaaggatctg 6420
ACCATTCCAACCCTTCGGGACGTTTCATTTGACCTACCGAAAGAACGGCGGTTCCTAGAC
NTP_II marker(6476,7264)>>>
|
BclI ORF_3 rf(2)(6473,7267)>>>
| | |
6421 atggcgcaggggatcaagctctgatcaagagacaggatgaggatcgtttcgcatgattga 6480
TACCGCGTCCCCTAGTTCGAGACTAGTTCTCTGTCCTACTCCTAGCAAAGCGTACTAACT
6481 acaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggctatga 6540
TGTTCTACCTAACGTGCGTCCAAGAGGCCGGCGAACCCACCTCTCCGATAAGCCGATACT
6541 ctgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggg 6600
GACCCGTGTTGTCTGTTAGCCGACGAGACTACGGCGGCACAAGGCCGACAGTCGCGTCCC
6601 gcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaagacga 6660
CGCGGGCCAAGAAAAACAGTTCTGGCTGGACAGGCCACGGGACTTACTTGACGTTCTGCT
6661 ggcagcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgt 6720
CCGTCGCGCCGATAGCACCGACCGGTGCTGCCCGCAAGGAACGCGTCGACACGAGCTGCA
6721 tgtcactgaagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcct 6780
ACAGTGACTTCGCCCTTCCCTGACCGACGATAACCCGCTTCACGGCCCCGTCCTAGAGGA
6781 gtcatctcaccttgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggct 6840
CAGTAGAGTGGAACGAGGACGGCTCTTTCATAGGTAGTACCGACTACGTTACGCCGCCGA
6841 gcatacgcttgatccggctacctgcccattcgaccaccaagcgaaacatcgcatcgagcg 6900
CGTATGCGAACTAGGCCGATGGACGGGTAAGCTGGTGGTTCGCTTTGTAGCGTAGCTCGC
6901 agcacgtactcggatggaagccggtcttgtcgatcaggatgatctggacgaagagcatca 6960
TCGTGCATGAGCCTACCTTCGGCCAGAACAGCTAGTCCTACTAGACCTGCTTCTCGTAGT
6961 ggggctcgcgccagccgaactgttcgccaggctcaaggcgagcatgcccgacggcgagga 7020
CCCCGAGCGCGGTCGGCTTGACAAGCGGTCCGAGTTCCGCTCGTACGGGCTGCCGCTCCT
7021 tctcgtcgtgacccatggcgatgcctgcttgccgaatatcatggtggaaaatggccgctt 7080
AGAGCAGCACTGGGTACCGCTACGGACGAACGGCTTATAGTACCACCTTTTACCGGCGAA
7081 ttctggattcatcgactgtggccggctgggtgtggcggaccgctatcaggacatagcgtt 7140
AAGACCTAAGTAGCTGACACCGGCCGACCCACACCGCCTGGCGATAGTCCTGTATCGCAA
7141 ggctacccgtgatattgctgaagagcttggcggcgaatgggctgaccgcttcctcgtgct 7200
CCGATGGGCACTATAACGACTTCTCGAACCGCCGCTTACCCGACTGGCGAAGGAGCACGA
7201 ttacggtatcgccgctcccgattcgcagcgcatcgccttctatcgccttcttgacgagtt 7260
AATGCCATAGCGGCGAGGGCTAAGCGTCGCGTAGCGGAAGATAGCGGAAGAACTGCTCAA
7261 cttctgaattttgttaaaatttttgttaaatcagctcattttttaaccaataggccgaaa 7320
GAAGACTTAAAACAATTTTAAAAACAATTTAGTCGAGTAAAAAATTGGTTATCCGGCTTT
f1 origin(7372,7678)<<<
|
7321 tcggcaaaatcccttataaatcaaaagaatagaccgagatagggttgagtgttgttccag 7380
AGCCGTTTTAGGGAATATTTAGTTTTCTTATCTGGCTCTATCCCAACTCACAACAAGGTC
7381 tttggaacaagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaaaccg 7440
AAACCTTGTTCTCAGGTGATAATTTCTTGCACCTGAGGTTGCAGTTTCCCGCTTTTTGGC
7441 tctatcagggcgatggcccactacgtgaaccatcaccctaatcaagttttttggggtcga 7500
AGATAGTCCCGCTACCGGGTGATGCACTTGGTAGTGGGATTAGTTCAAAAAACCCCAGCT
7501 ggtgccgtaaagcactaaatcggaaccctaaagggagcccccgatttagagcttgacggg 7560
CCACGGCATTTCGTGATTTAGCCTTGGGATTTCCCTCGGGGGCTAAATCTCGAACTGCCC
7561 gaaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgctaggg 7620
CTTTCGGCCGCTTGCACCGCTCTTTCCTTCCCTTCTTTCGCTTTCCTCGCCCGCGATCCC
7621 cgctggcaagtgtagcggtcacgctgcgcgtaaccaccacacccgccgcgcttaatgcgc 7680
GCGACCGTTCACATCGCCAGTGCGACGCGCATTGGTGGTGTGGGCGGCGCGAATTACGCG
RITR other(7733,7834)<<<
|
LITR other(7733,7834)>>>
|
7681 cgctacagggcgcgtccattcgccattcaggatcgaattaattcttaattaacatcatca 7740
GCGATGTCCCGCGCAGGTAAGCGGTAAGTCCTAGCTTAATTAAGAATTAATTGTAGTAGT
7741 ataatataccttttttggattgaagccaatatgataatgagggggtggagtttgtgacgt 7800
TATTATATGGAAAAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACACTGCA
7801 ggcgcggggcgtgggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgttgca 7860
CCGCGCCCCGCACCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTACAACGT
Encap other(7914,8062)>>>
|
7861 agtgtggcggaacacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgcc 7920
TCACACCGCCTTGTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGG
7921 ggtgtacacaggaagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgg 7980
CCACATGTGTCCTTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACC
7981 gcgtaaccgagtaagatttggccattttcgcgggaaaactgaataagaggaagtgaaatc 8040
CGCATTGGCTCATTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAG
8041 tgaataattttgtgttactcatagcgcgtaatactctcatgagcggatacatatttgaat 8100
ACTTATTAAAACACAATGAGTATCGCGCATTATGAGAGTACTCGCCTATGTATAAACTTA
8101 gtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctg 8160
CATAAATCTTTTTATTTGTTTATCCCCAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGAC
8161 acgtaactataacggtcctaaggtagcgaaaatgtagtcttatgcaatactcttgtagtc 8220
TGCATTGATATTGCCAGGATTCCATCGCTTTTACATCAGAATACGTTATGAGAACATCAG
8221 ttgcaacatggtaacgatgagttagcaacatgccttacaaggagagaaaaagcaccgtgc 8280
AACGTTGTACCATTGCTACTCAATCGTTGTACGGAATGTTCCTCTCTTTTTCGTGGCACG
8281 atgccgattggtggaagtaaggtggtacgat 8311
TACGGCTAACCACCTTCATTCCACCATGCTA
LOCUS dna 8311 bp
FEATURES Location/Qualifiers
Other Gene 109..289
/gene="HIV-1_5_LTR other"
Other Gene 400..444
/gene="HIV-1_psi_pack other"
misc_binding 486..491
/dbxref="REBASE:NruI"
Regulatory_Seq 951..1184
/gene="RRE reg"
ORF 994..1695
/sequence="ORF_1 rf(1)"
ORF 1775..2755
/sequence="ORF_4 rf(4)"
Promoter 1838..3015
/gene="EF1a prom"
misc_binding 3087..3092
/dbxref="REBASE:EcoRI"
misc_binding 3111..3116
/dbxref="REBASE:HincII"
misc_binding 3117..3122
/dbxref="REBASE:EcoRV"
misc_binding 3129..3136
/dbxref="REBASE:NotI"
misc_binding 3138..3143
/dbxref="REBASE:BamHI"
misc_binding 3144..3149
/dbxref="REBASE:XbaI"
Promoter 3206..3474
/gene="SV40 prom"
Rep_Origin 3373..3450
/gene="SV40 origin"
ORF 3440..4108
/sequence="ORF_2 rf(2)"
misc_binding 3453..3465
/dbxref="REBASE:SfiI"
Marker 3509..4108
/gene="puro marker"
Other Gene 4718..4770
/gene="delta_U3 other"
Other Gene 4771..4951
/gene="HIV-1_5_LTR other"
Rep_Origin 5458..6077
/gene="pBR322 origin"
Promoter 6335..6384
/gene="NEOKAN prom"
misc_binding 6442..6447
/dbxref="REBASE:BclI"
ORF 6473..7267
/sequence="ORF_3 rf(2)"
Marker 6476..7264
/gene="NTP_II marker"
Rep_Origin 7372..7678
/gene="f1 origin"
Other Gene 7733..7834
/gene="RITR other"
Other Gene 7733..7834
/gene="LITR other"
Other Gene 7914..8062
/gene="Encap other"
BASE COUNT 1974 a 2052 c 2371 g 1914 t 0 others