pLenti-III-miR-Off

                            FEATURES             Location/Qualifiers
     source          1..8322
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     LTR             109..289
                     /note="5' LTR"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    336..461
                     /note="HIV-1 psi"
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    951..1184
                     /note="HIV-1 RRE"
                     /note="The Rev response element (RRE) of human 
                     immunodeficiency virus type 1 allows for Rev-dependent mRNA
                     export from the nucleus to the cytoplasm."
                     /note="color: #ffe4c4"
     enhancer        2155..2460
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        2461..2664
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             2719..3435
                     /codon_start=1
                     /product="enhanced GFP"
                     /note="EGFP"
                     /note="mammalian codon-optimized"
                     /note="This feature has 3 segments:
                        1: 2719 .. 2721 / #05fd14
                        2: 2722 .. 2724 / #05fd14 / 1a
                        3: 2725 .. 3435 / #05fd14"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             3517..4119
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /note="PuroR"
                     /note="confers resistance to puromycin"
                     /note="color: #ccffcc"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    4132..4719
                     /note="WPRE"
                     /note="woodchuck posttranscriptional regulatory element"
                     /note="color: #ffffff"
     CDS             complement(4603..4614)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /note="Factor Xa site"
                     /note="color: #cc99b2"
                     /translation="IEGR"
     misc_feature    4729..4962
                     /note="3'LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     LTR             4782..4962
                     /note="5' LTR"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     polyA_signal    5034..5155
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      complement(5484..6072)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number colE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             6484..7278
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /note="NeoR/KanR"
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin)"
                     /note="color: #ccffcc"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
ORIGIN
        1 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
       61 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
      121 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
      181 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
      241 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
      301 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
      361 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
      421 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
      481 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
      541 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
      601 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
      661 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
      721 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
      781 agaccaccgc acagcaagcc cgctgatctt cagacctgga ggaggagata tgagggacat
      841 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
      901 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
      961 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     1021 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     1081 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     1141 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     1201 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     1261 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     1321 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     1381 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     1441 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     1501 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     1561 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     1621 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgaaagctt
     1681 gggattcgaa tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     1741 agacataata gcaacagaca tacaaactaa agaactacaa aaacaaatta caaaaattca
     1801 aaattttcgg gtttttcgaa cctagggcta gatacgtaga acgctgacgt catcaacccg
     1861 ctccaaggaa tcgcgggccc agtgtcacta ggcgggaaca cccagcgcgc gtgcgccctg
     1921 gcaggaagat ggctgtgagg gacaggggag tggcgccctg caatatttgc atgtcgctat
     1981 gtgttctggg aaatcaccat aaacgtgaaa tgtctttgga tttgggaatc ttataagttc
     2041 tgtatgagac cacaaaaata gtcttctcta gaagctagca gatatcagtc gacactcgag
     2101 caattgtagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
     2161 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
     2221 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     2281 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
     2341 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     2401 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     2461 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
     2521 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
     2581 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
     2641 tgggaggtct atataagcag agctggttta gtgaaccgtc agggtaccgg atcccttaag
     2701 tgaacaacta gtgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     2761 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
     2821 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     2881 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
     2941 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     3001 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     3061 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     3121 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
     3181 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
     3241 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     3301 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag
     3361 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     3421 gacgagctgt acaaggaatt cggaagcggg cagtgcacca actacgccct gctgaagctg
     3481 gccggcgacg tggagagcaa ccccggcccc ggatccatgg ccaccgagta caagcccacg
     3541 gtgcgcctcg ccacccgcga cgacgtcccc cgggccgtac gcaccctcgc cgccgcgttc
     3601 gccgactacc ccgccacgcg ccacaccgtc gacccggacc gccacatcga gcgggtcacc
     3661 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     3721 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     3781 ttcgccgaga tcggctcgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     3841 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     3901 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     3961 gtggaggcgg ccgagcgcgc tggggtgccc gccttcctgg agacctccgc gccccgcaac
     4021 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     4081 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgaa cgcgttccgg aaatcaacct
     4141 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     4201 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     4261 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     4321 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     4381 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     4441 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     4501 gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt
     4561 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     4621 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct cgccttcgcc
     4681 ctcagacgag tcggatctcc ctttgggccg cctccccgcc tgtccggatg gaagggctaa
     4741 ttcactccca acgaatacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     4801 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     4861 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     4921 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc
     4981 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
     5041 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     5101 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     5161 tctggcatct atgtcgggtg cggagaaaga ggtaatgaaa tggcattatg ggtattatgg
     5221 gtctgcatta atgaatcggc caacggatgt cggtgtgaaa taccgcacag atgcgtaagg
     5281 agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     5341 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     5401 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     5461 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     5521 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     5581 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     5641 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     5701 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     5761 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     5821 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     5881 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     5941 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     6001 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     6061 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     6121 aactcacgtt aagggatttt ggccatgaga ttatcaaaaa ggatcttcac ctagatcctt
     6181 ttcacgtaga aagccagtcc gcagaaacgg tgctgacccc ggatgaatgt cagctactgg
     6241 gctatctgga caagggaaaa cgcaagcgca aagagaaagc aggtagcttg cagtgggctt
     6301 acatggcgat agctagactg ggcggtttta tggacagcaa gcgaaccgga attgccagct
     6361 ggggcgccct ctggtaaggt tgggaagccc tgcaaagtaa actggatggc tttcttgccg
     6421 ccaaggatct gatggcgcag gggatcaagc tctgatcaag agacaggatg aggatcgttt
     6481 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
     6541 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
     6601 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
     6661 ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
     6721 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
     6781 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
     6841 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
     6901 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
     6961 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc
     7021 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
     7081 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
     7141 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
     7201 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
     7261 cttgacgagt tcttctgaat tttgttaaaa tttttgttaa atcagctcat tttttaacca
     7321 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
     7381 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
     7441 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
     7501 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
     7561 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
     7621 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
     7681 gcttaatgcg ccgctacagg gcgcgtccat tcgccattca ggatcgaatt aattcttaat
     7741 taacatcatc aataatatac cttttttgga ttgaagccaa tatgataatg agggggtgga
     7801 gtttgtgacg tggcgcgggg cgtgggaacg gggcgggtga cgtagtagtg tggcggaagt
     7861 gtgatgttgc aagtgtggcg gaacacatgt aagcgacgga tgtggcaaaa gtgacgtttt
     7921 tggtgtgcgc cggtgtacac aggaagtgac aattttcgcg cggttttagg cggatgttgt
     7981 agtaaatttg ggcgtaaccg agtaagattt ggccattttc gcgggaaaac tgaataagag
     8041 gaagtgaaat ctgaataatt ttgtgttact catagcgcgt aatactctca tgagcggata
     8101 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     8161 agtgccacct gacgtaacta taacggtcct aaggtagcga aaatgtagtc ttatgcaata
     8221 ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa
     8281 aagcaccgtg catgccgatt ggtggaagta aggtggtacg at
                        

                            FEATURES             Location/Qualifiers
     source          1..8322
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     LTR             109..289
                     /note="5' LTR"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    336..461
                     /note="HIV-1 psi"
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    951..1184
                     /note="HIV-1 RRE"
                     /note="The Rev response element (RRE) of human 
                     immunodeficiency virus type 1 allows for Rev-dependent mRNA
                     export from the nucleus to the cytoplasm."
                     /note="color: #ffe4c4"
     enhancer        2155..2460
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        2461..2664
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             2719..3435
                     /codon_start=1
                     /product="enhanced GFP"
                     /note="EGFP"
                     /note="mammalian codon-optimized"
                     /note="This feature has 3 segments:
                        1: 2719 .. 2721 / #05fd14
                        2: 2722 .. 2724 / #05fd14 / 1a
                        3: 2725 .. 3435 / #05fd14"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             3517..4119
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /note="PuroR"
                     /note="confers resistance to puromycin"
                     /note="color: #ccffcc"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    4132..4719
                     /note="WPRE"
                     /note="woodchuck posttranscriptional regulatory element"
                     /note="color: #ffffff"
     CDS             complement(4603..4614)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /note="Factor Xa site"
                     /note="color: #cc99b2"
                     /translation="IEGR"
     misc_feature    4729..4962
                     /note="3'LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     LTR             4782..4962
                     /note="5' LTR"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     polyA_signal    5034..5155
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      complement(5484..6072)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number colE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             6484..7278
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /note="NeoR/KanR"
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin)"
                     /note="color: #ccffcc"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"