pLenti-III-miR-Off-Blank
Vector Name | pLenti-III-miR-Off-Blank |
VectorType | Lentiviral Vector |
Antibiotic Information | Bacterial: Kanamycin Mammalian: Puromycin |
Sequencing Primers | |
Additional Information | No Additional Information |

***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg 61 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg 121 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct 181 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt 241 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga 301 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg 361 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga 421 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa 481 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa 541 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag 601 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat 661 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga 721 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta 781 agaccaccgc acagcaagcc cgctgatctt cagacctgga ggaggagata tgagggacat 841 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc 901 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg 961 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg 1021 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct 1081 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca 1141 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc 1201 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct 1261 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac 1321 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa 1381 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg 1441 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt 1501 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag 1561 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga 1621 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgaaagctt 1681 gggattcgaa tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 1741 agacataata gcaacagaca tacaaactaa agaactacaa aaacaaatta caaaaattca 1801 aaattttcgg gtttttcgaa cctagggcta gatacgtaga acgctgacgt catcaacccg 1861 ctccaaggaa tcgcgggccc agtgtcacta ggcgggaaca cccagcgcgc gtgcgccctg 1921 gcaggaagat ggctgtgagg gacaggggag tggcgccctg caatatttgc atgtcgctat 1981 gtgttctggg aaatcaccat aaacgtgaaa tgtctttgga tttgggaatc ttataagttc 2041 tgtatgagac cacaaaaata gtcttctcta gcgggtgaac tcacgtcaga agtcgagcaa 2101 ttgtagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca 2161 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca 2221 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg 2281 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg 2341 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc 2401 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg 2461 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca 2521 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt 2581 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg 2641 gaggtctata taagcagagc tggtttagtg aaccgtcagg gtaccggatc ccttaagtga 2701 acaactagtg ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc 2761 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag 2821 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc 2881 gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac 2941 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag 3001 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc 3061 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc 3121 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc 3181 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc 3241 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg 3301 ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag 3361 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac 3421 gagctgtaca aggaattcgg aagcgggcag tgcaccaact acgccctgct gaagctggcc 3481 ggcgacgtgg agagcaaccc cggccccgga tccatggcca ccgagtacaa gcccacggtg 3541 cgcctcgcca cccgcgacga cgtcccccgg gccgtacgca ccctcgccgc cgcgttcgcc 3601 gactaccccg ccacgcgcca caccgtcgac ccggaccgcc acatcgagcg ggtcaccgag 3661 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac 3721 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc 3781 gccgagatcg gctcgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag 3841 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc 3901 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg 3961 gaggcggccg agcgcgctgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc 4021 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg 4081 cgcacctggt gcatgacccg caagcccggt gcctgaacgc gttccggaaa tcaacctctg 4141 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta 4201 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt 4261 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc 4321 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt 4381 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg 4441 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac 4501 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc 4561 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac 4621 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtctcgc cttcgccctc 4681 agacgagtcg gatctccctt tgggccgcct ccccgcctgt ccggatggaa gggctaattc 4741 actcccaacg aatacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga 4801 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct 4861 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat 4921 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta 4981 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta 5041 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat 5101 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct 5161 ggcatctatg tcgggtgcgg agaaagaggt aatgaaatgg cattatgggt attatgggtc 5221 tgcattaatg aatcggccaa cggatgtcgg tgtgaaatac cgcacagatg cgtaaggaga 5281 aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt 5341 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca 5401 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa 5461 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat 5521 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc 5581 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc 5641 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt 5701 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac 5761 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg 5821 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca 5881 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc 5941 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa 6001 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa 6061 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac 6121 tcacgttaag ggattttggc catgagatta tcaaaaagga tcttcaccta gatccttttc 6181 acgtagaaag ccagtccgca gaaacggtgc tgaccccgga tgaatgtcag ctactgggct 6241 atctggacaa gggaaaacgc aagcgcaaag agaaagcagg tagcttgcag tgggcttaca 6301 tggcgatagc tagactgggc ggttttatgg acagcaagcg aaccggaatt gccagctggg 6361 gcgccctctg gtaaggttgg gaagccctgc aaagtaaact ggatggcttt cttgccgcca 6421 aggatctgat ggcgcagggg atcaagctct gatcaagaga caggatgagg atcgtttcgc 6481 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc 6541 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca 6601 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg 6661 caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg 6721 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag 6781 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg 6841 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc 6901 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa 6961 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac 7021 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat 7081 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac 7141 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc 7201 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt 7261 gacgagttct tctgaatttt gttaaaattt ttgttaaatc agctcatttt ttaaccaata 7321 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt 7381 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg 7441 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt 7501 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc 7561 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg 7621 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct 7681 taatgcgccg ctacagggcg cgtccattcg ccattcagga tcgaattaat tcttaattaa 7741 catcatcaat aatatacctt ttttggattg aagccaatat gataatgagg gggtggagtt 7801 tgtgacgtgg cgcggggcgt gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg 7861 atgttgcaag tgtggcggaa cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg 7921 tgtgcgccgg tgtacacagg aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt 7981 aaatttgggc gtaaccgagt aagatttggc cattttcgcg ggaaaactga ataagaggaa 8041 gtgaaatctg aataattttg tgttactcat agcgcgtaat actctcatga gcggatacat 8101 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt 8161 gccacctgac gtaactataa cggtcctaag gtagcgaaaa tgtagtctta tgcaatactc 8221 ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag 8281 caccgtgcat gccgattggt ggaagtaagg tggtacgat
FEATURES Location/Qualifiers source 1..8319 /organism="recombinant plasmid" /mol_type="other DNA" LTR 109..289 /note="5' LTR" /note="truncated 5' long terminal repeat (LTR) from HIV-1" /note="color: #ffe4c4" misc_feature 336..461 /note="HIV-1 psi" /note="packaging signal of human immunodeficiency virus type 1" /note="color: #ffe4c4" misc_feature 951..1184 /note="HIV-1 RRE" /note="The Rev response element (RRE) of human immunodeficiency virus type 1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /note="color: #ffe4c4" enhancer 2152..2457 /note="CMV enhancer" /note="human cytomegalovirus immediate early enhancer" /note="color: #ffffff" promoter 2458..2661 /note="CMV promoter" /note="human cytomegalovirus (CMV) immediate early promoter" /note="color: #ffffff; direction: RIGHT" CDS 2716..3432 /codon_start=1 /product="enhanced GFP" /note="EGFP" /note="mammalian codon-optimized" /note="This feature has 3 segments: 1: 2716 .. 2718 / #05fd14 2: 2719 .. 2721 / #05fd14 / 1a 3: 2722 .. 3432 / #05fd14" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 3514..4116 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /note="PuroR" /note="confers resistance to puromycin" /note="color: #ccffcc" /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" misc_feature 4129..4716 /note="WPRE" /note="woodchuck posttranscriptional regulatory element" /note="color: #ffffff" CDS complement(4600..4611) /codon_start=1 /product="Factor Xa recognition and cleavage site" /note="Factor Xa site" /note="color: #cc99b2" /translation="IEGR" misc_feature 4726..4959 /note="3'LTR (Delta-U3)" /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /note="color: #ffe4c4" LTR 4779..4959 /note="5' LTR" /note="truncated 5' long terminal repeat (LTR) from HIV-1" /note="color: #ffe4c4" polyA_signal 5031..5152 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" /note="color: #a6acb3" rep_origin complement(5481..6069) /direction=LEFT /note="ori" /note="high-copy-number colE1/pMB1/pBR322/pUC origin of replication" /note="color: #ffff00" CDS 6481..7275 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /note="NeoR/KanR" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin)" /note="color: #ccffcc" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" ORIGIN 1 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg 61 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg 121 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct 181 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt 241 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga 301 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg 361 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga 421 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa 481 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa 541 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag 601 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat 661 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga 721 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta 781 agaccaccgc acagcaagcc cgctgatctt cagacctgga ggaggagata tgagggacat 841 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc 901 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg 961 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg 1021 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct 1081 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca 1141 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc 1201 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct 1261 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac 1321 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa 1381 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg 1441 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt 1501 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag 1561 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga 1621 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgaaagctt 1681 gggattcgaa tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 1741 agacataata gcaacagaca tacaaactaa agaactacaa aaacaaatta caaaaattca 1801 aaattttcgg gtttttcgaa cctagggcta gatacgtaga acgctgacgt catcaacccg 1861 ctccaaggaa tcgcgggccc agtgtcacta ggcgggaaca cccagcgcgc gtgcgccctg 1921 gcaggaagat ggctgtgagg gacaggggag tggcgccctg caatatttgc atgtcgctat 1981 gtgttctggg aaatcaccat aaacgtgaaa tgtctttgga tttgggaatc ttataagttc 2041 tgtatgagac cacaaaaata gtcttctcta gcgggtgaac tcacgtcaga agtcgagcaa 2101 ttgtagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca 2161 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca 2221 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg 2281 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg 2341 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc 2401 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg 2461 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca 2521 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt 2581 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg 2641 gaggtctata taagcagagc tggtttagtg aaccgtcagg gtaccggatc ccttaagtga 2701 acaactagtg ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc 2761 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag 2821 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc 2881 gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac 2941 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag 3001 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc 3061 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc 3121 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc 3181 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc 3241 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg 3301 ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag 3361 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac 3421 gagctgtaca aggaattcgg aagcgggcag tgcaccaact acgccctgct gaagctggcc 3481 ggcgacgtgg agagcaaccc cggccccgga tccatggcca ccgagtacaa gcccacggtg 3541 cgcctcgcca cccgcgacga cgtcccccgg gccgtacgca ccctcgccgc cgcgttcgcc 3601 gactaccccg ccacgcgcca caccgtcgac ccggaccgcc acatcgagcg ggtcaccgag 3661 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac 3721 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc 3781 gccgagatcg gctcgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag 3841 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc 3901 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg 3961 gaggcggccg agcgcgctgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc 4021 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg 4081 cgcacctggt gcatgacccg caagcccggt gcctgaacgc gttccggaaa tcaacctctg 4141 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta 4201 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt 4261 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc 4321 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt 4381 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg 4441 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac 4501 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc 4561 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac 4621 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtctcgc cttcgccctc 4681 agacgagtcg gatctccctt tgggccgcct ccccgcctgt ccggatggaa gggctaattc 4741 actcccaacg aatacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga 4801 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct 4861 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat 4921 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta 4981 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta 5041 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat 5101 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct 5161 ggcatctatg tcgggtgcgg agaaagaggt aatgaaatgg cattatgggt attatgggtc 5221 tgcattaatg aatcggccaa cggatgtcgg tgtgaaatac cgcacagatg cgtaaggaga 5281 aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt 5341 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca 5401 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa 5461 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat 5521 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc 5581 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc 5641 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt 5701 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac 5761 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg 5821 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca 5881 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc 5941 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa 6001 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa 6061 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac 6121 tcacgttaag ggattttggc catgagatta tcaaaaagga tcttcaccta gatccttttc 6181 acgtagaaag ccagtccgca gaaacggtgc tgaccccgga tgaatgtcag ctactgggct 6241 atctggacaa gggaaaacgc aagcgcaaag agaaagcagg tagcttgcag tgggcttaca 6301 tggcgatagc tagactgggc ggttttatgg acagcaagcg aaccggaatt gccagctggg 6361 gcgccctctg gtaaggttgg gaagccctgc aaagtaaact ggatggcttt cttgccgcca 6421 aggatctgat ggcgcagggg atcaagctct gatcaagaga caggatgagg atcgtttcgc 6481 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc 6541 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca 6601 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg 6661 caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg 6721 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag 6781 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg 6841 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc 6901 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa 6961 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac 7021 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat 7081 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac 7141 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc 7201 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt 7261 gacgagttct tctgaatttt gttaaaattt ttgttaaatc agctcatttt ttaaccaata 7321 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt 7381 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg 7441 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt 7501 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc 7561 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg 7621 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct 7681 taatgcgccg ctacagggcg cgtccattcg ccattcagga tcgaattaat tcttaattaa 7741 catcatcaat aatatacctt ttttggattg aagccaatat gataatgagg gggtggagtt 7801 tgtgacgtgg cgcggggcgt gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg 7861 atgttgcaag tgtggcggaa cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg 7921 tgtgcgccgg tgtacacagg aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt 7981 aaatttgggc gtaaccgagt aagatttggc cattttcgcg ggaaaactga ataagaggaa 8041 gtgaaatctg aataattttg tgttactcat agcgcgtaat actctcatga gcggatacat 8101 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt 8161 gccacctgac gtaactataa cggtcctaag gtagcgaaaa tgtagtctta tgcaatactc 8221 ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag 8281 caccgtgcat gccgattggt ggaagtaagg tggtacgat
FEATURES Location/Qualifiers source 1..8319 /organism="recombinant plasmid" /mol_type="other DNA" LTR 109..289 /note="5' LTR" /note="truncated 5' long terminal repeat (LTR) from HIV-1" /note="color: #ffe4c4" misc_feature 336..461 /note="HIV-1 psi" /note="packaging signal of human immunodeficiency virus type 1" /note="color: #ffe4c4" misc_feature 951..1184 /note="HIV-1 RRE" /note="The Rev response element (RRE) of human immunodeficiency virus type 1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /note="color: #ffe4c4" enhancer 2152..2457 /note="CMV enhancer" /note="human cytomegalovirus immediate early enhancer" /note="color: #ffffff" promoter 2458..2661 /note="CMV promoter" /note="human cytomegalovirus (CMV) immediate early promoter" /note="color: #ffffff; direction: RIGHT" CDS 2716..3432 /codon_start=1 /product="enhanced GFP" /note="EGFP" /note="mammalian codon-optimized" /note="This feature has 3 segments: 1: 2716 .. 2718 / #05fd14 2: 2719 .. 2721 / #05fd14 / 1a 3: 2722 .. 3432 / #05fd14" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 3514..4116 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /note="PuroR" /note="confers resistance to puromycin" /note="color: #ccffcc" /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" misc_feature 4129..4716 /note="WPRE" /note="woodchuck posttranscriptional regulatory element" /note="color: #ffffff" CDS complement(4600..4611) /codon_start=1 /product="Factor Xa recognition and cleavage site" /note="Factor Xa site" /note="color: #cc99b2" /translation="IEGR" misc_feature 4726..4959 /note="3'LTR (Delta-U3)" /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /note="color: #ffe4c4" LTR 4779..4959 /note="5' LTR" /note="truncated 5' long terminal repeat (LTR) from HIV-1" /note="color: #ffe4c4" polyA_signal 5031..5152 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" /note="color: #a6acb3" rep_origin complement(5481..6069) /direction=LEFT /note="ori" /note="high-copy-number colE1/pMB1/pBR322/pUC origin of replication" /note="color: #ffff00" CDS 6481..7275 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /note="NeoR/KanR" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin)" /note="color: #ccffcc" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"