pLenti-III-miR-Off-Blank

Vector Name pLenti-III-miR-Off-Blank
VectorType Lentiviral Vector
Antibiotic Information Bacterial: Kanamycin
Mammalian: Puromycin
Sequencing Primers
Additional Information No Additional Information
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector only and does not include the gene of interest. The sequence of the gene can be found through the accession number link on the product page. The restriction sites within the cloning region may differ from the provided sequence.
        1 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
       61 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
      121 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
      181 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
      241 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
      301 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
      361 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
      421 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
      481 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
      541 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
      601 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
      661 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
      721 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
      781 agaccaccgc acagcaagcc cgctgatctt cagacctgga ggaggagata tgagggacat
      841 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
      901 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
      961 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     1021 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     1081 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     1141 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     1201 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     1261 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     1321 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     1381 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     1441 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     1501 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     1561 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     1621 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgaaagctt
     1681 gggattcgaa tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     1741 agacataata gcaacagaca tacaaactaa agaactacaa aaacaaatta caaaaattca
     1801 aaattttcgg gtttttcgaa cctagggcta gatacgtaga acgctgacgt catcaacccg
     1861 ctccaaggaa tcgcgggccc agtgtcacta ggcgggaaca cccagcgcgc gtgcgccctg
     1921 gcaggaagat ggctgtgagg gacaggggag tggcgccctg caatatttgc atgtcgctat
     1981 gtgttctggg aaatcaccat aaacgtgaaa tgtctttgga tttgggaatc ttataagttc
     2041 tgtatgagac cacaaaaata gtcttctcta gcgggtgaac tcacgtcaga agtcgagcaa
     2101 ttgtagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
     2161 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     2221 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     2281 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
     2341 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     2401 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
     2461 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
     2521 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
     2581 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
     2641 gaggtctata taagcagagc tggtttagtg aaccgtcagg gtaccggatc ccttaagtga
     2701 acaactagtg ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     2761 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
     2821 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     2881 gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac
     2941 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     3001 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     3061 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     3121 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
     3181 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
     3241 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     3301 ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag
     3361 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     3421 gagctgtaca aggaattcgg aagcgggcag tgcaccaact acgccctgct gaagctggcc
     3481 ggcgacgtgg agagcaaccc cggccccgga tccatggcca ccgagtacaa gcccacggtg
     3541 cgcctcgcca cccgcgacga cgtcccccgg gccgtacgca ccctcgccgc cgcgttcgcc
     3601 gactaccccg ccacgcgcca caccgtcgac ccggaccgcc acatcgagcg ggtcaccgag
     3661 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac
     3721 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc
     3781 gccgagatcg gctcgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag
     3841 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc
     3901 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg
     3961 gaggcggccg agcgcgctgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc
     4021 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg
     4081 cgcacctggt gcatgacccg caagcccggt gcctgaacgc gttccggaaa tcaacctctg
     4141 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     4201 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     4261 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     4321 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     4381 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     4441 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     4501 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc
     4561 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     4621 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtctcgc cttcgccctc
     4681 agacgagtcg gatctccctt tgggccgcct ccccgcctgt ccggatggaa gggctaattc
     4741 actcccaacg aatacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     4801 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     4861 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     4921 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta
     4981 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta
     5041 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     5101 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     5161 ggcatctatg tcgggtgcgg agaaagaggt aatgaaatgg cattatgggt attatgggtc
     5221 tgcattaatg aatcggccaa cggatgtcgg tgtgaaatac cgcacagatg cgtaaggaga
     5281 aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     5341 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     5401 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     5461 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     5521 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     5581 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     5641 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     5701 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     5761 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     5821 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     5881 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     5941 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     6001 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     6061 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     6121 tcacgttaag ggattttggc catgagatta tcaaaaagga tcttcaccta gatccttttc
     6181 acgtagaaag ccagtccgca gaaacggtgc tgaccccgga tgaatgtcag ctactgggct
     6241 atctggacaa gggaaaacgc aagcgcaaag agaaagcagg tagcttgcag tgggcttaca
     6301 tggcgatagc tagactgggc ggttttatgg acagcaagcg aaccggaatt gccagctggg
     6361 gcgccctctg gtaaggttgg gaagccctgc aaagtaaact ggatggcttt cttgccgcca
     6421 aggatctgat ggcgcagggg atcaagctct gatcaagaga caggatgagg atcgtttcgc
     6481 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     6541 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
     6601 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
     6661 caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
     6721 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     6781 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     6841 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     6901 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
     6961 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac
     7021 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     7081 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     7141 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     7201 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     7261 gacgagttct tctgaatttt gttaaaattt ttgttaaatc agctcatttt ttaaccaata
     7321 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
     7381 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
     7441 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
     7501 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
     7561 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
     7621 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
     7681 taatgcgccg ctacagggcg cgtccattcg ccattcagga tcgaattaat tcttaattaa
     7741 catcatcaat aatatacctt ttttggattg aagccaatat gataatgagg gggtggagtt
     7801 tgtgacgtgg cgcggggcgt gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg
     7861 atgttgcaag tgtggcggaa cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg
     7921 tgtgcgccgg tgtacacagg aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt
     7981 aaatttgggc gtaaccgagt aagatttggc cattttcgcg ggaaaactga ataagaggaa
     8041 gtgaaatctg aataattttg tgttactcat agcgcgtaat actctcatga gcggatacat
     8101 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     8161 gccacctgac gtaactataa cggtcctaag gtagcgaaaa tgtagtctta tgcaatactc
     8221 ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag
     8281 caccgtgcat gccgattggt ggaagtaagg tggtacgat
                            FEATURES             Location/Qualifiers
     source          1..8319
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     LTR             109..289
                     /note="5' LTR"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    336..461
                     /note="HIV-1 psi"
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    951..1184
                     /note="HIV-1 RRE"
                     /note="The Rev response element (RRE) of human 
                     immunodeficiency virus type 1 allows for Rev-dependent mRNA
                     export from the nucleus to the cytoplasm."
                     /note="color: #ffe4c4"
     enhancer        2152..2457
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        2458..2661
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             2716..3432
                     /codon_start=1
                     /product="enhanced GFP"
                     /note="EGFP"
                     /note="mammalian codon-optimized"
                     /note="This feature has 3 segments:
                        1: 2716 .. 2718 / #05fd14
                        2: 2719 .. 2721 / #05fd14 / 1a
                        3: 2722 .. 3432 / #05fd14"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             3514..4116
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /note="PuroR"
                     /note="confers resistance to puromycin"
                     /note="color: #ccffcc"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    4129..4716
                     /note="WPRE"
                     /note="woodchuck posttranscriptional regulatory element"
                     /note="color: #ffffff"
     CDS             complement(4600..4611)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /note="Factor Xa site"
                     /note="color: #cc99b2"
                     /translation="IEGR"
     misc_feature    4726..4959
                     /note="3'LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     LTR             4779..4959
                     /note="5' LTR"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     polyA_signal    5031..5152
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      complement(5481..6069)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number colE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             6481..7275
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /note="NeoR/KanR"
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin)"
                     /note="color: #ccffcc"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
ORIGIN
        1 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
       61 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
      121 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
      181 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
      241 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
      301 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
      361 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
      421 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
      481 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
      541 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
      601 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
      661 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
      721 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
      781 agaccaccgc acagcaagcc cgctgatctt cagacctgga ggaggagata tgagggacat
      841 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
      901 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
      961 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     1021 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     1081 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     1141 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     1201 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     1261 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     1321 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     1381 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     1441 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     1501 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     1561 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     1621 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgaaagctt
     1681 gggattcgaa tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     1741 agacataata gcaacagaca tacaaactaa agaactacaa aaacaaatta caaaaattca
     1801 aaattttcgg gtttttcgaa cctagggcta gatacgtaga acgctgacgt catcaacccg
     1861 ctccaaggaa tcgcgggccc agtgtcacta ggcgggaaca cccagcgcgc gtgcgccctg
     1921 gcaggaagat ggctgtgagg gacaggggag tggcgccctg caatatttgc atgtcgctat
     1981 gtgttctggg aaatcaccat aaacgtgaaa tgtctttgga tttgggaatc ttataagttc
     2041 tgtatgagac cacaaaaata gtcttctcta gcgggtgaac tcacgtcaga agtcgagcaa
     2101 ttgtagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
     2161 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     2221 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     2281 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
     2341 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     2401 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
     2461 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
     2521 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
     2581 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
     2641 gaggtctata taagcagagc tggtttagtg aaccgtcagg gtaccggatc ccttaagtga
     2701 acaactagtg ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     2761 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
     2821 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     2881 gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac
     2941 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     3001 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     3061 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     3121 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
     3181 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
     3241 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     3301 ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag
     3361 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     3421 gagctgtaca aggaattcgg aagcgggcag tgcaccaact acgccctgct gaagctggcc
     3481 ggcgacgtgg agagcaaccc cggccccgga tccatggcca ccgagtacaa gcccacggtg
     3541 cgcctcgcca cccgcgacga cgtcccccgg gccgtacgca ccctcgccgc cgcgttcgcc
     3601 gactaccccg ccacgcgcca caccgtcgac ccggaccgcc acatcgagcg ggtcaccgag
     3661 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac
     3721 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc
     3781 gccgagatcg gctcgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag
     3841 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc
     3901 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg
     3961 gaggcggccg agcgcgctgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc
     4021 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg
     4081 cgcacctggt gcatgacccg caagcccggt gcctgaacgc gttccggaaa tcaacctctg
     4141 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     4201 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     4261 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     4321 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     4381 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     4441 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     4501 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc
     4561 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     4621 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtctcgc cttcgccctc
     4681 agacgagtcg gatctccctt tgggccgcct ccccgcctgt ccggatggaa gggctaattc
     4741 actcccaacg aatacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     4801 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     4861 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     4921 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta
     4981 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta
     5041 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     5101 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     5161 ggcatctatg tcgggtgcgg agaaagaggt aatgaaatgg cattatgggt attatgggtc
     5221 tgcattaatg aatcggccaa cggatgtcgg tgtgaaatac cgcacagatg cgtaaggaga
     5281 aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     5341 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     5401 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     5461 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     5521 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     5581 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     5641 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     5701 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     5761 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     5821 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     5881 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     5941 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     6001 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     6061 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     6121 tcacgttaag ggattttggc catgagatta tcaaaaagga tcttcaccta gatccttttc
     6181 acgtagaaag ccagtccgca gaaacggtgc tgaccccgga tgaatgtcag ctactgggct
     6241 atctggacaa gggaaaacgc aagcgcaaag agaaagcagg tagcttgcag tgggcttaca
     6301 tggcgatagc tagactgggc ggttttatgg acagcaagcg aaccggaatt gccagctggg
     6361 gcgccctctg gtaaggttgg gaagccctgc aaagtaaact ggatggcttt cttgccgcca
     6421 aggatctgat ggcgcagggg atcaagctct gatcaagaga caggatgagg atcgtttcgc
     6481 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     6541 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
     6601 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
     6661 caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
     6721 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     6781 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     6841 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     6901 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
     6961 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac
     7021 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     7081 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     7141 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     7201 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     7261 gacgagttct tctgaatttt gttaaaattt ttgttaaatc agctcatttt ttaaccaata
     7321 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
     7381 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
     7441 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
     7501 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
     7561 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
     7621 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
     7681 taatgcgccg ctacagggcg cgtccattcg ccattcagga tcgaattaat tcttaattaa
     7741 catcatcaat aatatacctt ttttggattg aagccaatat gataatgagg gggtggagtt
     7801 tgtgacgtgg cgcggggcgt gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg
     7861 atgttgcaag tgtggcggaa cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg
     7921 tgtgcgccgg tgtacacagg aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt
     7981 aaatttgggc gtaaccgagt aagatttggc cattttcgcg ggaaaactga ataagaggaa
     8041 gtgaaatctg aataattttg tgttactcat agcgcgtaat actctcatga gcggatacat
     8101 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     8161 gccacctgac gtaactataa cggtcctaag gtagcgaaaa tgtagtctta tgcaatactc
     8221 ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag
     8281 caccgtgcat gccgattggt ggaagtaagg tggtacgat
                        
                            FEATURES             Location/Qualifiers
     source          1..8319
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     LTR             109..289
                     /note="5' LTR"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    336..461
                     /note="HIV-1 psi"
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    951..1184
                     /note="HIV-1 RRE"
                     /note="The Rev response element (RRE) of human 
                     immunodeficiency virus type 1 allows for Rev-dependent mRNA
                     export from the nucleus to the cytoplasm."
                     /note="color: #ffe4c4"
     enhancer        2152..2457
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        2458..2661
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             2716..3432
                     /codon_start=1
                     /product="enhanced GFP"
                     /note="EGFP"
                     /note="mammalian codon-optimized"
                     /note="This feature has 3 segments:
                        1: 2716 .. 2718 / #05fd14
                        2: 2719 .. 2721 / #05fd14 / 1a
                        3: 2722 .. 3432 / #05fd14"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             3514..4116
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /note="PuroR"
                     /note="confers resistance to puromycin"
                     /note="color: #ccffcc"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    4129..4716
                     /note="WPRE"
                     /note="woodchuck posttranscriptional regulatory element"
                     /note="color: #ffffff"
     CDS             complement(4600..4611)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /note="Factor Xa site"
                     /note="color: #cc99b2"
                     /translation="IEGR"
     misc_feature    4726..4959
                     /note="3'LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     LTR             4779..4959
                     /note="5' LTR"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     polyA_signal    5031..5152
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      complement(5481..6069)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number colE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             6481..7275
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /note="NeoR/KanR"
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin)"
                     /note="color: #ccffcc"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"