pLenti-III-mir
| Vector Name | pLenti-III-mir |
| VectorType | Lentiviral Vector |
| Antibiotic Information | Puromycin |
| Sequencing Primers | SV40 Reverse 5'-GACTTTCCACACCTGGTTGCTGAC-3' |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
61 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
121 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
181 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
241 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
301 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
361 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
421 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
481 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
541 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
601 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
661 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
721 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
781 agaccaccgc acagcaagcc cgctgatctt cagacctgga ggaggagata tgagggacat
841 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
901 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
961 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
1021 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
1081 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
1141 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
1201 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
1261 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
1321 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
1381 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
1441 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
1501 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
1561 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
1621 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgaaagctt
1681 gggattcgaa tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
1741 agacataata gcaacagaca tacaaactaa agaactacaa aaacaaatta caaaaattca
1801 aaattttcgg gtttttcgaa cctagggttc cgcgttacat aacttacggt aaatggcccg
1861 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
1921 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
1981 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
2041 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
2101 cagtacatct acgtttagtc atcgctatta ccatggtgat gcggttttgg cagtacatca
2161 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
2221 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg
2281 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc
2341 gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa
2401 gaaccgagtt taaactccct atcagtgata gagatctccc tatcagtgat agagagctag
2461 aatctagagg taccgatatc gaattcatag ctagccctgc aggtctagac tcgaggcggc
2521 cgcagtcgag tacccatacg acgtcccaga ctacgcttga gtttaaacac gcgtggtgtg
2581 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
2641 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
2701 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
2761 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
2821 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggccatg
2881 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccctcg ggccgtacgc
2941 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtgga cccggaccgc
3001 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
3061 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
3121 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
3181 cggctggccg cgcagcaaca gatggaaggg ctcctggcgc cgcaccggcc caaggagccc
3241 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc
3301 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
3361 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
3421 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaacg
3481 cgttccggaa atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
3541 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
3601 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
3661 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
3721 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
3781 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
3841 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca
3901 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
3961 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
4021 ccgcgtctcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg
4081 tccggatgga agggctaatt cactcccaac gaatacaaga tctgcttttt gcttgtactg
4141 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
4201 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
4261 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
4321 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
4381 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
4441 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
4501 tcaatgtatc ttatcatgtc tggcatctat gtcgggtgcg gagaaagagg taatgaaatg
4561 gcattatggg tattatgggt ctgcattaat gaatcggcca acggatgtcg gtgtgaaata
4621 ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact
4681 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
4741 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
4801 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
4861 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
4921 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
4981 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
5041 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
5101 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
5161 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
5221 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
5281 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
5341 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
5401 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
5461 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg ccatgagatt atcaaaaagg
5521 atcttcacct agatcctttt cacgtagaaa gccagtccgc agaaacggtg ctgaccccgg
5581 atgaatgtca gctactgggc tatctggaca agggaaaacg caagcgcaaa gagaaagcag
5641 gtagcttgca gtgggcttac atggcgatag ctagactggg cggttttatg gacagcaagc
5701 gaaccggaat tgccagctgg ggcgccctct ggtaaggttg ggaagccctg caaagtaaac
5761 tggatggctt tcttgccgcc aaggatctga tggcgcaggg gatcaagctc tgatcaagag
5821 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc
5881 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
5941 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
6001 tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg
6061 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
6121 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
6181 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
6241 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
6301 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
6361 ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
6421 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
6481 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
6541 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
6601 atcgccttct atcgccttct tgacgagttc ttctgaattt tgttaaaatt tttgttaaat
6661 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
6721 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
6781 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
6841 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
6901 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
6961 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
7021 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtccattc gccattcagg
7081 atcgaattaa ttcttaatta acatcatcaa taatatacct tttttggatt gaagccaata
7141 tgataatgag ggggtggagt ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg
7201 tagtagtgtg gcggaagtgt gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg
7261 tggcaaaagt gacgtttttg gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg
7321 gttttaggcg gatgttgtag taaatttggg cgtaaccgag taagatttgg ccattttcgc
7381 gggaaaactg aataagagga agtgaaatct gaataatttt gtgttactca tagcgcgtaa
7441 tactctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
7501 gcgcacattt ccccgaaaag tgccacctga cgtaactata acggtcctaa ggtagcgaaa
7561 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
7621 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
LOCUS dna 7680 bp
FEATURES Location/Qualifiers
Other Gene 109..289
/gene="HIV-1_5_LTR other"
Other Gene 400..444
/gene="HIV-1_psi_pack other"
misc_binding 486..491
/dbxref="REBASE:NruI"
Regulatory_Seq 951..1184
/gene="RRE reg"
ORF 994..1695
/sequence="ORF_1 rf(1)"
misc_binding 2005..2010
/dbxref="REBASE:NdeI"
Promoter 2279..2360
/gene="CMV prom"
Regulatory_Seq 2416..2455
/gene="tetO reg"
misc_binding 2469..2474
/dbxref="REBASE:KpnI"
misc_binding 2475..2480
/dbxref="REBASE:EcoRV"
misc_binding 2481..2486
/dbxref="REBASE:EcoRI"
misc_binding 2497..2502
/dbxref="REBASE:PstI"
misc_binding 2510..2515
/dbxref="REBASE:XhoI"
misc_binding 2516..2523
/dbxref="REBASE:NotI"
Promoter 2575..2843
/gene="SV40 prom"
Rep_Origin 2742..2819
/gene="SV40 origin"
ORF 2809..3477
/sequence="ORF_2 rf(1)"
Marker 2878..3477
/gene="puro marker"
Other Gene 4087..4139
/gene="delta_U3 other"
Other Gene 4140..4320
/gene="HIV-1_5_LTR other"
Rep_Origin 4827..5446
/gene="pBR322 origin"
Promoter 5704..5753
/gene="NEOKAN prom"
misc_binding 5811..5816
/dbxref="REBASE:BclI"
ORF 5842..6636
/sequence="ORF_3 rf(1)"
Marker 5845..6633
/gene="NTP_II marker"
Rep_Origin 6741..7047
/gene="f1 origin"
Other Gene 7102..7203
/gene="RITR other"
Other Gene 7102..7203
/gene="LITR other"
Other Gene 7283..7431
/gene="Encap other"
BASE COUNT 1930 a 1886 c 2099 g 1765 t 0 others
ORIGIN
1 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
61 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
121 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
181 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
241 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
301 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
361 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
421 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
481 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
541 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
601 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
661 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
721 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
781 agaccaccgc acagcaagcc cgctgatctt cagacctgga ggaggagata tgagggacat
841 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
901 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
961 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
1021 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
1081 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
1141 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
1201 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
1261 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
1321 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
1381 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
1441 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
1501 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
1561 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
1621 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgaaagctt
1681 gggattcgaa tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
1741 agacataata gcaacagaca tacaaactaa agaactacaa aaacaaatta caaaaattca
1801 aaattttcgg gtttttcgaa cctagggttc cgcgttacat aacttacggt aaatggcccg
1861 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
1921 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
1981 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
2041 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
2101 cagtacatct acgtttagtc atcgctatta ccatggtgat gcggttttgg cagtacatca
2161 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
2221 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg
2281 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc
2341 gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa
2401 gaaccgagtt taaactccct atcagtgata gagatctccc tatcagtgat agagagctag
2461 aatctagagg taccgatatc gaattcatag ctagccctgc aggtctagac tcgaggcggc
2521 cgcagtcgag tacccatacg acgtcccaga ctacgcttga gtttaaacac gcgtggtgtg
2581 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
2641 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
2701 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
2761 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
2821 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggccatg
2881 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccctcg ggccgtacgc
2941 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtgga cccggaccgc
3001 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
3061 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
3121 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
3181 cggctggccg cgcagcaaca gatggaaggg ctcctggcgc cgcaccggcc caaggagccc
3241 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc
3301 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
3361 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
3421 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaacg
3481 cgttccggaa atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
3541 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
3601 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
3661 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
3721 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
3781 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
3841 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca
3901 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
3961 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
4021 ccgcgtctcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg
4081 tccggatgga agggctaatt cactcccaac gaatacaaga tctgcttttt gcttgtactg
4141 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
4201 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
4261 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
4321 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
4381 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
4441 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
4501 tcaatgtatc ttatcatgtc tggcatctat gtcgggtgcg gagaaagagg taatgaaatg
4561 gcattatggg tattatgggt ctgcattaat gaatcggcca acggatgtcg gtgtgaaata
4621 ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact
4681 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
4741 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
4801 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
4861 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
4921 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
4981 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
5041 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
5101 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
5161 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
5221 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
5281 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
5341 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
5401 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
5461 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg ccatgagatt atcaaaaagg
5521 atcttcacct agatcctttt cacgtagaaa gccagtccgc agaaacggtg ctgaccccgg
5581 atgaatgtca gctactgggc tatctggaca agggaaaacg caagcgcaaa gagaaagcag
5641 gtagcttgca gtgggcttac atggcgatag ctagactggg cggttttatg gacagcaagc
5701 gaaccggaat tgccagctgg ggcgccctct ggtaaggttg ggaagccctg caaagtaaac
5761 tggatggctt tcttgccgcc aaggatctga tggcgcaggg gatcaagctc tgatcaagag
5821 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc
5881 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
5941 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
6001 tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg
6061 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
6121 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
6181 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
6241 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
6301 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
6361 ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
6421 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
6481 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
6541 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
6601 atcgccttct atcgccttct tgacgagttc ttctgaattt tgttaaaatt tttgttaaat
6661 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
6721 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
6781 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
6841 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
6901 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
6961 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
7021 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtccattc gccattcagg
7081 atcgaattaa ttcttaatta acatcatcaa taatatacct tttttggatt gaagccaata
7141 tgataatgag ggggtggagt ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg
7201 tagtagtgtg gcggaagtgt gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg
7261 tggcaaaagt gacgtttttg gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg
7321 gttttaggcg gatgttgtag taaatttggg cgtaaccgag taagatttgg ccattttcgc
7381 gggaaaactg aataagagga agtgaaatct gaataatttt gtgttactca tagcgcgtaa
7441 tactctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
7501 gcgcacattt ccccgaaaag tgccacctga cgtaactata acggtcctaa ggtagcgaaa
7561 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
7621 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
//
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 cgtgccttattaggaaggcaacagacgggtctgacatggattggacgaaccactgaattg 60
GCACGGAATAATCCTTCCGTTGTCTGCCCAGACTGTACCTAACCTGCTTGGTGACTTAAC
HIV-1_5_LTR other(109,289)>>>
|
61 ccgcattgcagagatattgtatttaagtgcctagctcgatacataaacgggtctctctgg 120
GGCGTAACGTCTCTATAACATAAATTCACGGATCGAGCTATGTATTTGCCCAGAGAGACC
121 ttagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcct 180
AATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAATTCGGA
181 caataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggt 240
GTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTGAGACCA
241 aactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggcgcccga 300
TTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCACCGCGGGCT
301 acagggacttgaaagcgaaagggaaaccagaggagctctctcgacgcaggactcggcttg 360
TGTCCCTGAACTTTCGCTTTCCCTTTGGTCTCCTCGAGAGAGCTGCGTCCTGAGCCGAAC
HIV-1_psi_pack other(400,444)>>>
|
361 ctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaattttga 420
GACTTCGCGCGTGCCGTTCTCCGCTCCCCGCCGCTGACCACTCATGCGGTTTTTAAAACT
421 ctagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcgggggagaa 480
GATCGCCTCCGATCTTCCTCTCTCTACCCACGCTCTCGCAGTCATAATTCGCCCCCTCTT
NruI
|
481 ttagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatataaattaa 540
AATCTAGCGCTACCCTTTTTTAAGCCAATTCCGGTCCCCCTTTCTTTTTTATATTTAATT
541 aacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcctgttag 600
TTGTATATCATACCCGTTCGTCCCTCGATCTTGCTAAGCGTCAATTAGGACCGGACAATC
601 aaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcagacaggat 660
TTTGTAGTCTTCCGACATCTGTTTATGACCCTGTCGATGTTGGTAGGGAAGTCTGTCCTA
661 cagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatcaaagga 720
GTCTTCTTGAATCTAGTAATATATTATGTCATCGTTGGGAGATAACACACGTAGTTTCCT
721 tagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaacaaaagta 780
ATCTCTATTTTCTGTGGTTCCTTCGAAATCTGTTCTATCTCCTTCTCGTTTTGTTTTCAT
781 agaccaccgcacagcaagcccgctgatcttcagacctggaggaggagatatgagggacat 840
TCTGGTGGCGTGTCGTTCGGGCGACTAGAAGTCTGGACCTCCTCCTCTATACTCCCTGTA
841 tggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagtagcaccc 900
ACCTCTTCACTTAATATATTTATATTTCATCATTTTTAACTTGGTAATCCTCATCGTGGG
RRE reg(951,1184)>>>
|
901 accaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaataggagctttg 960
TGGTTCCGTTTCTCTTCTCACCACGTCTCTCTTTTTTCTCGTCACCCTTATCCTCGAAAC
ORF_1 rf(1)(994,1695)>>>
|
961 ttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacgctgacg 1020
AAGGAACCCAAGAACCCTCGTCGTCCTTCGTGATACCCGCGTCGCAGTTACTGCGACTGC
1021 gtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctgagggct 1080
CATGTCCGGTCTGTTAATAACAGACCATATCACGTCGTCGTCTTGTTAAACGACTCCCGA
1081 attgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctccaggca 1140
TAACTCCGCGTTGTCGTAGACAACGTTGAGTGTCAGACCCCGTAGTTCGTCGAGGTCCGT
1141 agaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttggggttgc 1200
TCTTAGGACCGACACCTTTCTATGGATTTCCTAGTTGTCGAGGACCCCTAAACCCCAACG
1201 tctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaataaatct 1260
AGACCTTTTGAGTAAACGTGGTGACGACACGGAACCTTACGATCAACCTCATTATTTAGA
1261 ctggaacagatttggaatcacacgacctggatggagtgggacagagaaattaacaattac 1320
GACCTTGTCTAAACCTTAGTGTGCTGGACCTACCTCACCCTGTCTCTTTAATTGTTAATG
1321 acaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaatgaacaa 1380
TGTTCGAATTATGTGAGGAATTAACTTCTTAGCGTTTTGGTCGTTCTTTTCTTACTTGTT
1381 gaattattggaattagataaatgggcaagtttgtggaattggtttaacataacaaattgg 1440
CTTAATAACCTTAATCTATTTACCCGTTCAAACACCTTAACCAAATTGTATTGTTTAACC
1441 ctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaagaatagtt 1500
GACACCATATATTTTAATAAGTATTACTATCATCCTCCGAACCATCCAAATTCTTATCAA
1501 tttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcgtttcag 1560
AAACGACATGAAAGATATCACTTATCTCAATCCGTCCCTATAAGTGGTAATAGCAAAGTC
1561 acccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaaggtgga 1620
TGGGTGGAGGGTTGGGGCTCCCCTGGGCTGTCCGGGCTTCCTTATCTTCTTCTTCCACCT
1621 gagagagacagagacagatccattcgattagtgaacggatctcgacggtatcgaaagctt 1680
CTCTCTCTGTCTCTGTCTAGGTAAGCTAATCACTTGCCTAGAGCTGCCATAGCTTTCGAA
1681 gggattcgaatttaaaagaaaaggggggattggggggtacagtgcaggggaaagaatagt 1740
CCCTAAGCTTAAATTTTCTTTTCCCCCCTAACCCCCCATGTCACGTCCCCTTTCTTATCA
1741 agacataatagcaacagacatacaaactaaagaactacaaaaacaaattacaaaaattca 1800
TCTGTATTATCGTTGTCTGTATGTTTGATTTCTTGATGTTTTTGTTTAATGTTTTTAAGT
1801 aaattttcgggtttttcgaacctagggttccgcgttacataacttacggtaaatggcccg 1860
TTTAAAAGCCCAAAAAGCTTGGATCCCAAGGCGCAATGTATTGAATGCCATTTACCGGGC
1861 cctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccata 1920
GGACCGACTGGCGGGTTGCTGGGGGCGGGTAACTGCAGTTATTACTGCATACAAGGGTAT
1921 gtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcc 1980
CATTGCGGTTATCCCTGAAAGGTAACTGCAGTTACCCACCTCATAAATGCCATTTGACGG
NdeI
|
1981 cacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgac 2040
GTGAACCGTCATGTAGTTCACATAGTATACGGTTCATGCGGGGGATAACTGCAGTTACTG
2041 ggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttgg 2100
CCATTTACCGGGCGGACCGTAATACGGGTCATGTACTGGAATACCCTGAAAGGATGAACC
2101 cagtacatctacgtttagtcatcgctattaccatggtgatgcggttttggcagtacatca 2160
GTCATGTAGATGCAAATCAGTAGCGATAATGGTACCACTACGCCAAAACCGTCATGTAGT
2161 atgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtca 2220
TACCCGCACCTATCGCCAAACTGAGTGCCCCTAAAGGTTCAGAGGTGGGGTAACTGCAGT
CMV prom(2279,2360)>>>
|
2221 atgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccg 2280
TACCCTCAAACAAAACCGTGGTTTTAGTTGCCCTGAAAGGTTTTACAGCATTGTTGAGGC
2281 ccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctc 2340
GGGGTAACTGCGTTTACCCGCCATCCGCACATGCCACCCTCCAGATATATTCGTCTCGAG
2341 gtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagaa 2400
CAAATCACTTGGCAGTCTAGCGGACCTCTGCGGTAGGTGCGACAAAACTGGAGGTATCTT
tetO reg(2416,2455)>>>
|
2401 gaaccgagtttaaactccctatcagtgatagagatctccctatcagtgatagagagctag 2460
CTTGGCTCAAATTTGAGGGATAGTCACTATCTCTAGAGGGATAGTCACTATCTCTCGATC
EcoRV
|
KpnI EcoRI PstI XhoI NotI
| | | | | |
2461 aatctagaggtaccgatatcgaattcatagctagccctgcaggtctagactcgaggcggc 2520
TTAGATCTCCATGGCTATAGCTTAAGTATCGATCGGGACGTCCAGATCTGAGCTCCGCCG
SV40 prom(2575,2843)>>>
|
2521 cgcagtcgagtacccatacgacgtcccagactacgcttgagtttaaacacgcgtggtgtg 2580
GCGTCAGCTCATGGGTATGCTGCAGGGTCTGATGCGAACTCAAATTTGTGCGCACCACAC
2581 gaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcag 2640
CTTTCAGGGGTCCGAGGGGTCGTCCGTCTTCATACGTTTCGTACGTAGAGTTAATCAGTC
2641 caaccaggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatc 2700
GTTGGTCCACACCTTTCAGGGGTCCGAGGGGTCGTCCGTCTTCATACGTTTCGTACGTAG
SV40 origin(2742,2819)>>>
|
2701 tcaattagtcagcaaccatagtcccgcccctaactccgcccatcccgcccctaactccgc 2760
AGTTAATCAGTCGTTGGTATCAGGGCGGGGATTGAGGCGGGTAGGGCGGGGATTGAGGCG
ORF_2 rf(1)(2809,3477)>>>
|
2761 ccagttccgcccattctccgccccatggctgactaattttttttatttatgcagaggccg 2820
GGTCAAGGCGGGTAAGAGGCGGGGTACCGACTGATTAAAAAAAATAAATACGTCTCCGGC
SfiI puro marker(2878,3477)>>>
| |
2821 aggccgcctcggcctctgagctattccagaagtagtgaggaggcttttttggaggccatg 2880
TCCGGCGGAGCCGGAGACTCGATAAGGTCTTCATCACTCCTCCGAAAAAACCTCCGGTAC
2881 accgagtacaagcccacggtgcgcctcgccacccgcgacgacgtccctcgggccgtacgc 2940
TGGCTCATGTTCGGGTGCCACGCGGAGCGGTGGGCGCTGCTGCAGGGAGCCCGGCATGCG
2941 accctcgccgccgcgttcgccgactaccccgccacgcgccacaccgtggacccggaccgc 3000
TGGGAGCGGCGGCGCAAGCGGCTGATGGGGCGGTGCGCGGTGTGGCACCTGGGCCTGGCG
3001 cacatcgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggctcgacatc 3060
GTGTAGCTCGCCCAGTGGCTCGACGTTCTTGAGAAGGAGTGCGCGCAGCCCGAGCTGTAG
3061 ggcaaggtgtgggtcgcggacgacggcgccgcggtggcggtctggaccacgccggagagc 3120
CCGTTCCACACCCAGCGCCTGCTGCCGCGGCGCCACCGCCAGACCTGGTGCGGCCTCTCG
3121 gtcgaagcgggggcggtgttcgccgagatcggcccgcgcatggccgagttgagcggttcc 3180
CAGCTTCGCCCCCGCCACAAGCGGCTCTAGCCGGGCGCGTACCGGCTCAACTCGCCAAGG
3181 cggctggccgcgcagcaacagatggaagggctcctggcgccgcaccggcccaaggagccc 3240
GCCGACCGGCGCGTCGTTGTCTACCTTCCCGAGGACCGCGGCGTGGCCGGGTTCCTCGGG
3241 gcgtggttcctggccaccgtcggcgtctcgcccgaccaccagggcaagggtctgggcagc 3300
CGCACCAAGGACCGGTGGCAGCCGCAGAGCGGGCTGGTGGTCCCGTTCCCAGACCCGTCG
3301 gccgtcgtgctccccggagtggaggcggccgagcgcgccggggtgcccgccttcctggag 3360
CGGCAGCACGAGGGGCCTCACCTCCGCCGGCTCGCGCGGCCCCACGGGCGGAAGGACCTC
3361 acctccgcgccccgcaacctccccttctacgagcggctcggcttcaccgtcaccgccgac 3420
TGGAGGCGCGGGGCGTTGGAGGGGAAGATGCTCGCCGAGCCGAAGTGGCAGTGGCGGCTG
3421 gtcgaggtgcccgaaggaccgcgcacctggtgcatgacccgcaagcccggtgcctgaacg 3480
CAGCTCCACGGGCTTCCTGGCGCGTGGACCACGTACTGGGCGTTCGGGCCACGGACTTGC
3481 cgttccggaaatcaacctctggattacaaaatttgtgaaagattgactggtattcttaac 3540
GCAAGGCCTTTAGTTGGAGACCTAATGTTTTAAACACTTTCTAACTGACCATAAGAATTG
3541 tatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgctatt 3600
ATACAACGAGGAAAATGCGATACACCTATGCGACGAAATTACGGAAACATAGTACGATAA
3601 gcttcccgtatggctttcattttctcctccttgtataaatcctggttgctgtctctttat 3660
CGAAGGGCATACCGAAAGTAAAAGAGGAGGAACATATTTAGGACCAACGACAGAGAAATA
3661 gaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgacgca 3720
CTCCTCAACACCGGGCAACAGTCCGTTGCACCGCACCACACGTGACACAAACGACTGCGT
3721 acccccactggttggggcattgccaccacctgtcagctcctttccgggactttcgctttc 3780
TGGGGGTGACCAACCCCGTAACGGTGGTGGACAGTCGAGGAAAGGCCCTGAAAGCGAAAG
3781 cccctccctattgccacggcggaactcatcgccgcctgccttgcccgctgctggacaggg 3840
GGGGAGGGATAACGGTGCCGCCTTGAGTAGCGGCGGACGGAACGGGCGACGACCTGTCCC
3841 gctcggctgttgggcactgacaattccgtggtgttgtcggggaagctgacgtcctttcca 3900
CGAGCCGACAACCCGTGACTGTTAAGGCACCACAACAGCCCCTTCGACTGCAGGAAAGGT
3901 tggctgctcgcctgtgttgccacctggattctgcgcgggacgtccttctgctacgtccct 3960
ACCGACGAGCGGACACAACGGTGGACCTAAGACGCGCCCTGCAGGAAGACGATGCAGGGA
3961 tcggccctcaatccagcggaccttccttcccgcggcctgctgccggctctgcggcctctt 4020
AGCCGGGAGTTAGGTCGCCTGGAAGGAAGGGCGCCGGACGACGGCCGAGACGCCGGAGAA
4021 ccgcgtctcgccttcgccctcagacgagtcggatctccctttgggccgcctccccgcctg 4080
GGCGCAGAGCGGAAGCGGGAGTCTGCTCAGCCTAGAGGGAAACCCGGCGGAGGGGCGGAC
delta_U3 other(4087,4139)>>>
|
4081 tccggatggaagggctaattcactcccaacgaatacaagatctgctttttgcttgtactg 4140
AGGCCTACCTTCCCGATTAAGTGAGGGTTGCTTATGTTCTAGACGAAAAACGAACATGAC
HIV-1_5_LTR other(4140,4320)>>>
|
4141 ggtctctctggttagaccagatctgagcctgggagctctctggctaactagggaacccac 4200
CCAGAGAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTG
4201 tgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgt 4260
ACGAATTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACA
4261 gtgactctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagca 4320
CACTGAGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGT
4321 gtagtagttcatgtcatcttattattcagtatttataacttgcaaagaaatgaatatcag 4380
CATCATCAAGTACAGTAGAATAATAAGTCATAAATATTGAACGTTTCTTTACTTATAGTC
4381 agagtgagaggaacttgtttattgcagcttataatggttacaaataaagcaatagcatca 4440
TCTCACTCTCCTTGAACAAATAACGTCGAATATTACCAATGTTTATTTCGTTATCGTAGT
4441 caaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactca 4500
GTTTAAAGTGTTTATTTCGTAAAAAAAGTGACGTAAGATCAACACCAAACAGGTTTGAGT
4501 tcaatgtatcttatcatgtctggcatctatgtcgggtgcggagaaagaggtaatgaaatg 4560
AGTTACATAGAATAGTACAGACCGTAGATACAGCCCACGCCTCTTTCTCCATTACTTTAC
4561 gcattatgggtattatgggtctgcattaatgaatcggccaacggatgtcggtgtgaaata 4620
CGTAATACCCATAATACCCAGACGTAATTACTTAGCCGGTTGCCTACAGCCACACTTTAT
4621 ccgcacagatgcgtaaggagaaaataccgcatcaggcgctcttccgcttcctcgctcact 4680
GGCGTGTCTACGCATTCCTCTTTTATGGCGTAGTCCGCGAGAAGGCGAAGGAGCGAGTGA
4681 gactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggta 4740
CTGAGCGACGCGAGCCAGCAAGCCGACGCCGCTCGCCATAGTCGAGTGAGTTTCCGCCAT
4741 atacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccag 4800
TATGCCAATAGGTGTCTTAGTCCCCTATTGCGTCCTTTCTTGTACACTCGTTTTCCGGTC
pBR322 origin(4827,5446)<<<
|
4801 caaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccc 4860
GTTTTCCGGTCCTTGGCATTTTTCCGGCGCAACGACCGCAAAAAGGTATCCGAGGCGGGG
4861 cctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggacta 4920
GGACTGCTCGTAGTGTTTTTAGCTGCGAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGAT
4921 taaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctg 4980
ATTTCTATGGTCCGCAAAGGGGGACCTTCGAGGGAGCACGCGAGAGGACAAGGCTGGGAC
4981 ccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagc 5040
GGCGAATGGCCTATGGACAGGCGGAAAGAGGGAAGCCCTTCGCACCGCGAAAGAGTATCG
5041 tcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcac 5100
AGTGCGACATCCATAGAGTCAAGCCACATCCAGCAAGCGAGGTTCGACCCGACACACGTG
5101 gaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaac 5160
CTTGGGGGGCAAGTCGGGCTGGCGACGCGGAATAGGCCATTGATAGCAGAACTCAGGTTG
5161 ccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcg 5220
GGCCATTCTGTGCTGAATAGCGGTGACCGTCGTCGGTGACCATTGTCCTAATCGTCTCGC
5221 aggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactaga 5280
TCCATACATCCGCCACGATGTCTCAAGAACTTCACCACCGGATTGATGCCGATGTGATCT
5281 aggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggt 5340
TCCTGTCATAAACCATAGACGCGAGACGACTTCGGTCAATGGAAGCCTTTTTCTCAACCA
5341 agctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcag 5400
TCGAGAACTAGGCCGTTTGTTTGGTGGCGACCATCGCCACCAAAAAAACAAACGTTCGTC
5401 cagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtct 5460
GTCTAATGCGCGTCTTTTTTTCCTAGAGTTCTTCTAGGAAACTAGAAAAGATGCCCCAGA
5461 gacgctcagtggaacgaaaactcacgttaagggattttggccatgagattatcaaaaagg 5520
CTGCGAGTCACCTTGCTTTTGAGTGCAATTCCCTAAAACCGGTACTCTAATAGTTTTTCC
5521 atcttcacctagatccttttcacgtagaaagccagtccgcagaaacggtgctgaccccgg 5580
TAGAAGTGGATCTAGGAAAAGTGCATCTTTCGGTCAGGCGTCTTTGCCACGACTGGGGCC
5581 atgaatgtcagctactgggctatctggacaagggaaaacgcaagcgcaaagagaaagcag 5640
TACTTACAGTCGATGACCCGATAGACCTGTTCCCTTTTGCGTTCGCGTTTCTCTTTCGTC
5641 gtagcttgcagtgggcttacatggcgatagctagactgggcggttttatggacagcaagc 5700
CATCGAACGTCACCCGAATGTACCGCTATCGATCTGACCCGCCAAAATACCTGTCGTTCG
NEOKAN prom(5704,5753)>>>
|
5701 gaaccggaattgccagctggggcgccctctggtaaggttgggaagccctgcaaagtaaac 5760
CTTGGCCTTAACGGTCGACCCCGCGGGAGACCATTCCAACCCTTCGGGACGTTTCATTTG
BclI
|
5761 tggatggctttcttgccgccaaggatctgatggcgcaggggatcaagctctgatcaagag 5820
ACCTACCGAAAGAACGGCGGTTCCTAGACTACCGCGTCCCCTAGTTCGAGACTAGTTCTC
NTP_II marker(5845,6633)>>>
|
ORF_3 rf(1)(5842,6636)>>>
| |
5821 acaggatgaggatcgtttcgcatgattgaacaagatggattgcacgcaggttctccggcc 5880
TGTCCTACTCCTAGCAAAGCGTACTAACTTGTTCTACCTAACGTGCGTCCAAGAGGCCGG
5881 gcttgggtggagaggctattcggctatgactgggcacaacagacaatcggctgctctgat 5940
CGAACCCACCTCTCCGATAAGCCGATACTGACCCGTGTTGTCTGTTAGCCGACGAGACTA
5941 gccgccgtgttccggctgtcagcgcaggggcgcccggttctttttgtcaagaccgacctg 6000
CGGCGGCACAAGGCCGACAGTCGCGTCCCCGCGGGCCAAGAAAAACAGTTCTGGCTGGAC
6001 tccggtgccctgaatgaactgcaagacgaggcagcgcggctatcgtggctggccacgacg 6060
AGGCCACGGGACTTACTTGACGTTCTGCTCCGTCGCGCCGATAGCACCGACCGGTGCTGC
6061 ggcgttccttgcgcagctgtgctcgacgttgtcactgaagcgggaagggactggctgcta 6120
CCGCAAGGAACGCGTCGACACGAGCTGCAACAGTGACTTCGCCCTTCCCTGACCGACGAT
6121 ttgggcgaagtgccggggcaggatctcctgtcatctcaccttgctcctgccgagaaagta 6180
AACCCGCTTCACGGCCCCGTCCTAGAGGACAGTAGAGTGGAACGAGGACGGCTCTTTCAT
6181 tccatcatggctgatgcaatgcggcggctgcatacgcttgatccggctacctgcccattc 6240
AGGTAGTACCGACTACGTTACGCCGCCGACGTATGCGAACTAGGCCGATGGACGGGTAAG
6241 gaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaagccggtcttgtc 6300
CTGGTGGTTCGCTTTGTAGCGTAGCTCGCTCGTGCATGAGCCTACCTTCGGCCAGAACAG
6301 gatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactgttcgccagg 6360
CTAGTCCTACTAGACCTGCTTCTCGTAGTCCCCGAGCGCGGTCGGCTTGACAAGCGGTCC
6361 ctcaaggcgagcatgcccgacggcgaggatctcgtcgtgacccatggcgatgcctgcttg 6420
GAGTTCCGCTCGTACGGGCTGCCGCTCCTAGAGCAGCACTGGGTACCGCTACGGACGAAC
6421 ccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggt 6480
GGCTTATAGTACCACCTTTTACCGGCGAAAAGACCTAAGTAGCTGACACCGGCCGACCCA
6481 gtggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggc 6540
CACCGCCTGGCGATAGTCCTGTATCGCAACCGATGGGCACTATAACGACTTCTCGAACCG
6541 ggcgaatgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgc 6600
CCGCTTACCCGACTGGCGAAGGAGCACGAAATGCCATAGCGGCGAGGGCTAAGCGTCGCG
6601 atcgccttctatcgccttcttgacgagttcttctgaattttgttaaaatttttgttaaat 6660
TAGCGGAAGATAGCGGAAGAACTGCTCAAGAAGACTTAAAACAATTTTAAAAACAATTTA
6661 cagctcattttttaaccaataggccgaaatcggcaaaatcccttataaatcaaaagaata 6720
GTCGAGTAAAAAATTGGTTATCCGGCTTTAGCCGTTTTAGGGAATATTTAGTTTTCTTAT
f1 origin(6741,7047)<<<
|
6721 gaccgagatagggttgagtgttgttccagtttggaacaagagtccactattaaagaacgt 6780
CTGGCTCTATCCCAACTCACAACAAGGTCAAACCTTGTTCTCAGGTGATAATTTCTTGCA
6781 ggactccaacgtcaaagggcgaaaaaccgtctatcagggcgatggcccactacgtgaacc 6840
CCTGAGGTTGCAGTTTCCCGCTTTTTGGCAGATAGTCCCGCTACCGGGTGATGCACTTGG
6841 atcaccctaatcaagttttttggggtcgaggtgccgtaaagcactaaatcggaaccctaa 6900
TAGTGGGATTAGTTCAAAAAACCCCAGCTCCACGGCATTTCGTGATTTAGCCTTGGGATT
6901 agggagcccccgatttagagcttgacggggaaagccggcgaacgtggcgagaaaggaagg 6960
TCCCTCGGGGGCTAAATCTCGAACTGCCCCTTTCGGCCGCTTGCACCGCTCTTTCCTTCC
6961 gaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcggtcacgctgcgcgt 7020
CTTCTTTCGCTTTCCTCGCCCGCGATCCCGCGACCGTTCACATCGCCAGTGCGACGCGCA
7021 aaccaccacacccgccgcgcttaatgcgccgctacagggcgcgtccattcgccattcagg 7080
TTGGTGGTGTGGGCGGCGCGAATTACGCGGCGATGTCCCGCGCAGGTAAGCGGTAAGTCC
RITR other(7102,7203)<<<
|
LITR other(7102,7203)>>>
|
7081 atcgaattaattcttaattaacatcatcaataatataccttttttggattgaagccaata 7140
TAGCTTAATTAAGAATTAATTGTAGTAGTTATTATATGGAAAAAACCTAACTTCGGTTAT
7141 tgataatgagggggtggagtttgtgacgtggcgcggggcgtgggaacggggcgggtgacg 7200
ACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCACCCTTGCCCCGCCCACTGC
7201 tagtagtgtggcggaagtgtgatgttgcaagtgtggcggaacacatgtaagcgacggatg 7260
ATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTTGTGTACATTCGCTGCCTAC
Encap other(7283,7431)>>>
|
7261 tggcaaaagtgacgtttttggtgtgcgccggtgtacacaggaagtgacaattttcgcgcg 7320
ACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCCTTCACTGTTAAAAGCGCGC
7321 gttttaggcggatgttgtagtaaatttgggcgtaaccgagtaagatttggccattttcgc 7380
CAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCATTCTAAACCGGTAAAAGCG
7381 gggaaaactgaataagaggaagtgaaatctgaataattttgtgttactcatagcgcgtaa 7440
CCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAACACAATGAGTATCGCGCATT
7441 tactctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttcc 7500
ATGAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCCCAAGG
7501 gcgcacatttccccgaaaagtgccacctgacgtaactataacggtcctaaggtagcgaaa 7560
CGCGTGTAAAGGGGCTTTTCACGGTGGACTGCATTGATATTGCCAGGATTCCATCGCTTT
7561 atgtagtcttatgcaatactcttgtagtcttgcaacatggtaacgatgagttagcaacat 7620
TACATCAGAATACGTTATGAGAACATCAGAACGTTGTACCATTGCTACTCAATCGTTGTA
7621 gccttacaaggagagaaaaagcaccgtgcatgccgattggtggaagtaaggtggtacgat 7680
CGGAATGTTCCTCTCTTTTTCGTGGCACGTACGGCTAACCACCTTCATTCCACCATGCTA
LOCUS dna 7680 bp
FEATURES Location/Qualifiers
Other Gene 109..289
/gene="HIV-1_5_LTR other"
Other Gene 400..444
/gene="HIV-1_psi_pack other"
misc_binding 486..491
/dbxref="REBASE:NruI"
Regulatory_Seq 951..1184
/gene="RRE reg"
ORF 994..1695
/sequence="ORF_1 rf(1)"
misc_binding 2005..2010
/dbxref="REBASE:NdeI"
Promoter 2279..2360
/gene="CMV prom"
Regulatory_Seq 2416..2455
/gene="tetO reg"
misc_binding 2469..2474
/dbxref="REBASE:KpnI"
misc_binding 2475..2480
/dbxref="REBASE:EcoRV"
misc_binding 2481..2486
/dbxref="REBASE:EcoRI"
misc_binding 2497..2502
/dbxref="REBASE:PstI"
misc_binding 2510..2515
/dbxref="REBASE:XhoI"
misc_binding 2516..2523
/dbxref="REBASE:NotI"
Promoter 2575..2843
/gene="SV40 prom"
Rep_Origin 2742..2819
/gene="SV40 origin"
ORF 2809..3477
/sequence="ORF_2 rf(1)"
misc_binding 2822..2834
/dbxref="REBASE:SfiI"
Marker 2878..3477
/gene="puro marker"
Other Gene 4087..4139
/gene="delta_U3 other"
Other Gene 4140..4320
/gene="HIV-1_5_LTR other"
Rep_Origin 4827..5446
/gene="pBR322 origin"
Promoter 5704..5753
/gene="NEOKAN prom"
misc_binding 5811..5816
/dbxref="REBASE:BclI"
ORF 5842..6636
/sequence="ORF_3 rf(1)"
Marker 5845..6633
/gene="NTP_II marker"
Rep_Origin 6741..7047
/gene="f1 origin"
Other Gene 7102..7203
/gene="RITR other"
Other Gene 7102..7203
/gene="LITR other"
Other Gene 7283..7431
/gene="Encap other"
BASE COUNT 1930 a 1886 c 2099 g 1765 t 0 others