pLenti-SFFV-dCas9-VPR
| Vector Name | pLenti-SFFV-dCas9-VPR |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa2401 aattcaaaat tttcgggttt ttcgaaccta ggtgcagtaa cgccattttg caaggcatgg2461 aaaaatacca aaccaagaat agagaagttc agatcaaggg cgggtacatg aaaatagcta2521 acgttgggcc aaacaggata tctgcggtga gcagtttcgg ccccggcccg gggccaagaa2581 cagatggtca ccgcagtttc ggccccggcc cgaggccaag aacagatggt ccccagatat2641 ggcccaaccc tcagcagttt cttaagaccc atcagatgtt tccaggctcc cccaaggacc2701 tgaaatgacc ctgcgcctta tttgaattaa ccaatcagcc tgcttctcgc ttctgttcgc2761 gcgcttctgc ttcccgagct ctataaaaga gctcacaacc cctcactcgg cgcgccagtc2821 ctccgacaga ctgagtcgcc cggctagcaa taattttgtt taactttaag aaggagatat2881 accatggaca agaagtactc cattgggctc gcgatcggca caaacagcgt cggctgggcc2941 gtcattacgg acgagtacaa ggtgccgagc aaaaaattca aagttctggg caataccgat3001 cgccacagca taaagaagaa cctcattggc gccctcctgt tcgactccgg ggagacggcc3061 gaagccacgc ggctcaaaag aacagcacgg cgcagatata cccgcagaaa gaatcggatc3121 tgctacctgc aggagatctt tagtaatgag atggctaagg tggatgactc tttcttccat3181 aggctggagg agtccttttt ggtggaggag gataaaaagc acgagcgcca cccaatcttt3241 ggcaatatcg tggacgaggt ggcgtaccat gaaaagtacc caaccatata tcatctgagg3301 aagaagcttg tagacagtac tgataaggct gacttgcggt tgatctatct cgcgctggcg3361 catatgatca aatttcgggg acacttcctc atcgaggggg acctgaaccc agacaacagc3421 gatgtcgaca aactctttat ccaactggtt cagacttaca atcagctttt cgaagagaac3481 ccgatcaacg catccggagt tgacgccaaa gcaatcctga gcgctaggct gtccaaatcc3541 cggcggctcg aaaacctcat cgcacagctc cctggggaga agaagaacgg cctgtttggt3601 aatcttatcg ccctgtcact cgggctgacc cccaacttta aatctaactt cgacctggcc3661 gaagatgcca agcttcaact gagcaaagac acctacgatg atgatctcga caatctgctg3721 gcccagatcg gcgaccagta cgcagacctt tttttggcgg caaagaacct gtcagacgcc3781 attctgctga gtgatattct gcgagtgaac acggagatca ccaaagctcc gctgagcgct3841 agtatgatca agcgctatga tgagcaccac caagacttga ctttgctgaa ggcccttgtc3901 agacagcaac tgcctgagaa gtacaaggaa attttcttcg atcagtctaa aaatggctac3961 gccggataca ttgacggcgg agcaagccag gaggaatttt acaaatttat taagcccatc4021 ttggaaaaaa tggacggcac cgaggagctg ctggtaaagc ttaacagaga agatctgttg4081 cgcaaacagc gcactttcga caatggaagc atcccccacc agattcacct gggcgaactg4141 cacgctatcc tcaggcggca agaggatttc tacccctttt tgaaagataa cagggaaaag4201 attgagaaaa tcctcacatt tcggataccc tactatgtag gccccctcgc ccggggaaat4261 tccagattcg cgtggatgac tcgcaaatca gaagagacca tcactccctg gaacttcgag4321 gaagtcgtgg ataagggggc ctctgcccag tccttcatcg aaaggatgac taactttgat4381 aaaaatctgc ctaacgaaaa ggtgcttcct aaacactctc tgctgtacga gtacttcaca4441 gtttataacg agctcaccaa ggtcaaatac gtcacagaag ggatgagaaa gccagcattc4501 ctgtctggag agcagaagaa agctatcgtg gacctcctct tcaagacgaa ccggaaagtt4561 accgtgaaac agctcaaaga agactatttc aaaaagattg aatgtttcga ctctgttgaa4621 atcagcggag tggaggatcg cttcaacgca tccctgggaa cgtatcacga tctcctgaaa4681 atcattaaag acaaggactt cctggacaat gaggagaacg aggacattct tgaggacatt4741 gtcctcaccc ttacgttgtt tgaagatagg gagatgattg aagaacgctt gaaaacttac4801 gctcatctct tcgacgacaa agtcatgaaa cagctcaaga ggcgccgata tacaggatgg4861 gggcggctgt caagaaaact gatcaatggg atccgagaca agcagagtgg aaagacaatc4921 ctggattttc ttaagtccga tggatttgcc aaccggaact tcatgcagtt gatccatgat4981 gactctctca cctttaagga ggacatccag aaagcacaag tttctggcca gggggacagt5041 cttcacgagc acatcgctaa tcttgcaggt agcccagcta tcaaaaaggg aatactgcag5101 accgttaagg tcgtggatga actcgtcaaa gtaatgggaa ggcataagcc cgagaatatc5161 gttatcgaga tggcccgaga gaaccaaact acccagaagg gacagaagaa cagtagggaa5221 aggatgaaga ggattgaaga gggtataaaa gaactggggt cccaaatcct taaggaacac5281 ccagttgaaa acacccagct tcagaatgag aagctctacc tgtactacct gcagaacggc5341 agggacatgt acgtggatca ggaactggac atcaatcggc tctccgacta cgacgtggat5401 gcgatcgtgc cccagtcttt tctcaaagat gattctattg ataataaagt gttgacaaga5461 tccgataaaa atagagggaa gagtgataac gtcccctcag aagaagttgt caagaaaatg5521 aaaaattatt ggcggcagct gctgaacgcc aaactgatca cacaacggaa gttcgataat5581 ctgactaagg ctgaacgagg tggcctgtct gagttggata aagccggctt catcaaaagg5641 cagcttgttg agacacgcca gatcaccaag cacgtggccc aaattctcga ttcacgcatg5701 aacaccaagt acgatgaaaa tgacaaactg attcgagagg tgaaagttat tactctgaag5761 tctaagctgg tctcagattt cagaaaggac tttcagtttt ataaggtgag agagatcaac5821 aattaccacc atgcgcatga tgcctacctg aatgcagtgg taggcactgc acttatcaaa5881 aaatatccca agcttgaatc tgaatttgtt tacggagact ataaagtgta cgatgttagg5941 aaaatgatcg caaagtctga gcaggaaata ggcaaggcca ccgctaagta cttcttttac6001 agcaatatta tgaatttttt caagaccgag attacactgg ccaatggaga gattcggaag6061 cgaccactta tcgaaacaaa cggagaaaca ggagaaatcg tgtgggacaa gggtagggat6121 ttcgcgacag tccggaaggt cctgtccatg ccgcaggtga acatcgttaa aaagaccgaa6181 gtacagaccg gaggcttctc caaggaaagt atcctcccga aaaggaacag cgacaagctg6241 atcgcacgca aaaaagattg ggaccccaag aaatacggcg gattcgattc tcctacagtc6301 gcttacagtg tactggttgt ggccaaagtg gagaaaggga agtctaaaaa actcaaaagc6361 gtcaaggaac tgctgggcat cacaatcatg gagcgatcaa gcttcgaaaa aaaccccatc6421 gactttctcg aggcgaaagg atataaagag gtcaaaaaag acctcatcat taagcttccc6481 aagtactctc tctttgagct tgaaaacggc cggaaacgaa tgctcgctag tgcgggcgag6541 ctgcagaaag gtaacgagct ggcactgccc tctaaatacg ttaatttctt gtatctggcc6601 agccactatg aaaagctcaa agggtctccc gaagataatg agcagaagca gctgttcgtg6661 gaacaacaca aacactacct tgatgagatc atcgagcaaa taagcgaatt ctccaaaaga6721 gtgatcctcg ccgacgctaa cctcgataag gtgctttctg cttacaataa gcacagggat6781 aagcccatca gggagcaggc agaaaacatt atccacttgt ttactctgac caacttgggc6841 gcgcctgcag ccttcaagta cttcgacacc accatagaca gaaagcggta cacctctaca6901 aaggaggtcc tggacgccac actgattcat cagtcaatta cggggctcta tgaaacaaga6961 atcgacctct ctcagctcgg tggagacagc agggctgacc ccaagaagaa gaggaaggtg7021 tcgccaggga tccgtcgact tgacgcgttg atatcaacaa gtttgtacaa aaaagcaggc7081 tacaaagagg ccagcggttc cggacgggct gacgcattgg acgattttga tctggatatg7141 ctgggaagtg acgccctcga tgattttgac cttgacatgc ttggttcgga tgcccttgat7201 gactttgacc tcgacatgct cggcagtgac gcccttgatg atttcgacct ggacatgctg7261 attaactcaa gaagttccgg atctccgaaa aagaaacgca aagttggtag ccagtacctg7321 cccgacaccg acgaccggca ccggatcgag gaaaagcgga agcggaccta cgagacattc7381 aagagcatca tgaagaagtc ccccttcagc ggccccaccg accctagacc tccacctaga7441 agaatcgccg tgcccagcag atccagcgcc agcgtgccaa aacctgcccc ccagccttac7501 cccttcacca gcagcctgag caccatcaac tacgacgagt tccctaccat ggtgttcccc7561 agcggccaga tctctcaggc ctctgctctg gctccagccc ctcctcaggt gctgcctcag7621 gctcctgctc ctgcaccagc tccagccatg gtgtctgcac tggctcaggc accagcaccc7681 gtgcctgtgc tggctcctgg acctccacag gctgtggctc caccagcccc taaacctaca7741 caggccggcg agggcacact gtctgaagct ctgctgcagc tgcagttcga cgacgaggat7801 ctgggagccc tgctgggaaa cagcaccgat cctgccgtgt tcaccgacct ggccagcgtg7861 gacaacagcg agttccaaca gctgctgaac cagggcatcc ctgtggcccc tcacaccacc7921 gagcccatgc tgatggaata ccccgaggcc atcacccggc tcgtgacagg cgctcagagg7981 cctcctgatc cagctcctgc ccctctggga gcaccaggcc tgcctaatgg actgctgtct8041 ggcgacgagg acttcagctc tatcgccgat atggatttct cagccttgct gggctctggc8101 agcggcagcc gggattccag ggaagggatg tttttgccga agcctgaggc cggctccgct8161 attagtgacg tgtttgaggg ccgcgaggtg tgccagccaa aacgaatccg gccatttcat8221 cctccaggaa gtccatgggc caaccgccca ctccccgcca gcctcgcacc aacaccaacc8281 ggtccagtac atgagccagt cgggtcactg accccggcac cagtccctca gccactggat8341 ccagcgcccg cagtgactcc cgaggccagt cacctgttgg aggatcccga tgaagagacg8401 agccaggctg tcaaagccct tcgggagatg gccgatactg tgattcccca gaaggaagag8461 gctgcaatct gtggccaaat ggacctttcc catccgcccc caaggggcca tctggatgag8521 ctgacaacca cacttgagtc catgaccgag gatctgaacc tggactcacc cctgaccccg8581 gaattgaacg agattctgga taccttcctg aacgacgagt gcctcttgca tgccatgcat8641 atcagcacag gactgtccat cttcgacaca tctctgttta ctagtccaaa aaagaaaaga8701 aaggtgtgag tttaaacacg cgtggtgtgg aaagtcccca ggctccccag caggcagaag8761 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc8821 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct8881 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg8941 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa9001 gtagtgagga ggcttttttg gaggccatga ccgagtacaa gcccacggtg cgcctcgcca9061 cccgcgacga cgtccctcgg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg9121 ccacgcgcca caccgtggac ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac9181 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg9241 cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg9301 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaagggc9361 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc9421 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg9481 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg9541 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt9601 gcatgacccg caagcccggt gcctgaggta ccacgcgttc cggaaatcaa cctctggatt9661 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg9721 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct9781 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc9841 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca9901 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac9961 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt10021 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct10081 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc10141 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tctcgccttc gccctcagac10201 gagtcggatc tccctttggg ccgcctcccc gcctgtccgg atggaagggc taattcactc10261 ccaacgaata caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg10321 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc10381 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct10441 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat10501 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc10561 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt10621 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggca10681 tctatgtcgg gtgcggagaa agaggtaatg aaatggcatt atgggtatta tgggtctgca10741 ttaatgaatc ggccaacgat cccggtgtga aataccgcac agatgcgtaa ggagaaaata10801 ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct10861 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga10921 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc10981 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg11041 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg11101 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt11161 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt11221 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg11281 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact11341 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt11401 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct11461 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac11521 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc11581 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg11641 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta11701 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca11761 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc11821 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc11881 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc11941 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat12001 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt12061 tgaaaaagga tcttcaccta gatccttttc acgtagaaag ccagtccgca gaaacggtgc12121 tgaccccgga tgaatgtcag ctactgggct atctggacaa gggaaaacgc aagcgcaaag12181 agaaagcagg tagcttgcag tgggcttaca tggcgatagc tagactgggc ggttttatgg12241 acagcaagcg aaccggaatt gccagctggg gcgccctctg gtaaggttgg gaagccctgc12301 aaagtaaact ggatggcttt ctcgccgcca aggatctgat ggcgcagggg atcaagctct12361 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt12421 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc12481 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag12541 accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg12601 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac12661 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc12721 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc12781 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc12841 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg12901 ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat12961 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc13021 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa13081 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat13141 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgaatttt gttaaaattt13201 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaacatcc cttataaatc13261 aaaagaatag accgcgatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt13321 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact13381 acgtgaacca tcacccaaat caagtttttt gcggtcgagg tgccgtaaag ctctaaatcg13441 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag13501 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac13561 gctgcgcgta accaccacac ccgcgcgctt aatgcgccgc tacagggcgc gtccattcgc13621 cattcaggat cgaattaatt cttaattaac atcatcaata atatacctt