pLenti-UTR-GFP
| Vector Name | pLenti-UTR-GFP |
| VectorType | Lentiviral Vector |
| Antibiotic Information | Puromycin |
| Sequencing Primers | 5'-CTCTCGGCATGGACGAGCTGTAC-3' |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg 61 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg 121 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct 181 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt 241 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga 301 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg 361 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga 421 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa 481 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa 541 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag 601 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat 661 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga 721 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta 781 agaccaccgc acagcaagcc cgctgatctt cagacctgga ggaggagata tgagggacat 841 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc 901 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg 961 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg 1021 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct 1081 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca 1141 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc 1201 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct 1261 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac 1321 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa 1381 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg 1441 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt 1501 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag 1561 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga 1621 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgaaagctt 1681 gggattcgaa tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 1741 agacataata gcaacagaca tacaaactaa agaactacaa aaacaaatta caaaaattca 1801 aaattttcgg gtttttcgaa cctagggttc cgcgttacat aacttacggt aaatggcccg 1861 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata 1921 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc 1981 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac 2041 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg 2101 cagtacatct acgtttagtc atcgctatta ccatggtgat gcggttttgg cagtacatca 2161 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca 2221 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg 2281 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc 2341 gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa 2401 gaaccgagtt taaactccct atcagtgata gagatctccc tatcagtgat agagagctag 2461 ccccgggatc gatcaattgc cggtcgccac catggtgagc aagggcgagg agctgttcac 2521 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt 2581 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac 2641 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca 2701 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc 2761 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg 2821 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga 2881 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa 2941 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca 3001 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg 3061 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa 3121 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat 3181 cactctcggc atggacgagc tgtacaagta aagtacgtag gtaccccagt gtggtggcct 3241 gcaggtgaat tcactagtac cggtaggcct gtcgacgata tcgggcccgc ggccgctgga 3301 tcctctagac tgcagctcga gtacccatac gacgtcccag actacgcttg agtttaaaca 3361 cgcgtggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct 3421 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca 3481 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc 3541 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta 3601 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt 3661 tggaggccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtccctc 3721 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtgg 3781 acccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg 3841 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca 3901 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt 3961 tgagcggttc ccggctggcc gcgcagcaac agatggaagg gctcctggcg ccgcaccggc 4021 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg 4081 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg 4141 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg 4201 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg 4261 gtgcctgaac gcgttccgga aatcaacctc tggattacaa aatttgtgaa agattgactg 4321 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt 4381 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc 4441 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt 4501 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga 4561 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct 4621 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga 4681 cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct 4741 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc 4801 tgcggcctct tccgcgtctc gccttcgccc tcagacgagt cggatctccc tttgggccgc 4861 ctccccgcct gtccggatgg aagggctaat tcactcccaa cgaatacaag atctgctttt 4921 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact 4981 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc 5041 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa 5101 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa 5161 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag 5221 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt 5281 gtccaaactc atcaatgtat cttatcatgt ctggcatcta tgtcgggtgc ggagaaagag 5341 gtaatgaaat ggcattatgg gtattatggg tctgcattaa tgaatcggcc aacggatgtc 5401 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt 5461 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact 5521 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag 5581 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata 5641 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 5701 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 5761 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc 5821 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 5881 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc 5941 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga 6001 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg 6061 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 6121 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg 6181 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt 6241 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gccatgagat 6301 tatcaaaaag gatcttcacc tagatccttt tcacgtagaa agccagtccg cagaaacggt 6361 gctgaccccg gatgaatgtc agctactggg ctatctggac aagggaaaac gcaagcgcaa 6421 agagaaagca ggtagcttgc agtgggctta catggcgata gctagactgg gcggttttat 6481 ggacagcaag cgaaccggaa ttgccagctg gggcgccctc tggtaaggtt gggaagccct 6541 gcaaagtaaa ctggatggct ttcttgccgc caaggatctg atggcgcagg ggatcaagct 6601 ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag 6661 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg 6721 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca 6781 agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc 6841 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg 6901 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg 6961 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta 7021 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag 7081 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac 7141 tgttcgccag gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg 7201 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg 7261 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg 7321 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg 7381 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgaatt ttgttaaaat 7441 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa 7501 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta 7561 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca 7621 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat 7681 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg 7741 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc 7801 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtccatt 7861 cgccattcag gatcgaatta attcttaatt aacatcatca ataatatacc ttttttggat 7921 tgaagccaat atgataatga gggggtggag tttgtgacgt ggcgcggggc gtgggaacgg 7981 ggcgggtgac gtagtagtgt ggcggaagtg tgatgttgca agtgtggcgg aacacatgta 8041 agcgacggat gtggcaaaag tgacgttttt ggtgtgcgcc ggtgtacaca ggaagtgaca 8101 attttcgcgc ggttttaggc ggatgttgta gtaaatttgg gcgtaaccga gtaagatttg 8161 gccattttcg cgggaaaact gaataagagg aagtgaaatc tgaataattt tgtgttactc 8221 atagcgcgta atactctcat gagcggatac atatttgaat gtatttagaa aaataaacaa 8281 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtaactat aacggtccta 8341 aggtagcgaa aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga 8401 gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa 8461 ggtggtacga t
FEATURES Location/Qualifiers source 1..8471 /organism="recombinant plasmid" /mol_type="other DNA" LTR 109..289 /note="5' LTR" /note="truncated 5' long terminal repeat (LTR) from HIV-1" /note="color: #ffe4c4" misc_feature 336..461 /note="HIV-1 psi" /note="packaging signal of human immunodeficiency virus type 1" /note="color: #ffe4c4" misc_feature 951..1184 /note="HIV-1 RRE" /note="The Rev response element (RRE) of human immunodeficiency virus type 1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /note="color: #ffe4c4" enhancer 1831..2135 /note="CMV enhancer" /note="human cytomegalovirus immediate early enhancer" /note="color: #ffffff" promoter 2136..2339 /note="CMV promoter" /note="human cytomegalovirus (CMV) immediate early promoter" /note="color: #ffffff; direction: RIGHT" protein_bind 2416..2434 /gene="tetO" /bound_moiety="tetracycline repressor TetR" /note="tet operator" /note=" " /note="color: #31849b" protein_bind 2437..2455 /gene="tetO" /bound_moiety="tetracycline repressor TetR" /note="tet operator" /note=" " /note="color: #31849b" CDS 2492..3211 /codon_start=1 /product="enhanced GFP" /note="EGFP" /note="mammalian codon-optimized" /note="This feature has 3 segments: 1: 2492 .. 2494 / #05fd14 2: 2495 .. 2497 / #05fd14 / 1a 3: 2498 .. 3211 / #05fd14" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 3322..3348 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /note="HA" /note="color: #cc99b2" /translation="YPYDVPDYA" rep_origin 3533..3668 /note="SV40 ori" /note="SV40 origin of replication" /note="color: #ffff00" CDS 3669..4268 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /note="PuroR" /note="confers resistance to puromycin" /note="color: #ccffcc" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" misc_feature 4281..4868 /note="WPRE" /note="woodchuck posttranscriptional regulatory element" /note="color: #ffffff" CDS complement(4752..4763) /codon_start=1 /product="Factor Xa recognition and cleavage site" /note="Factor Xa site" /note="color: #cc99b2" /translation="IEGR" misc_feature 4878..5111 /note="3'LTR (Delta-U3)" /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /note="color: #ffe4c4" LTR 4931..5111 /note="5' LTR" /note="truncated 5' long terminal repeat (LTR) from HIV-1" /note="color: #ffe4c4" polyA_signal 5183..5304 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" /note="color: #a6acb3" rep_origin complement(5633..6221) /direction=LEFT /note="ori" /note="high-copy-number colE1/pMB1/pBR322/pUC origin of replication" /note="color: #ffff00" CDS 6633..7427 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /note="NeoR/KanR" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin)" /note="color: #ccffcc" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" ORIGIN 1 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg 61 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg 121 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct 181 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt 241 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga 301 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg 361 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga 421 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa 481 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa 541 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag 601 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat 661 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga 721 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta 781 agaccaccgc acagcaagcc cgctgatctt cagacctgga ggaggagata tgagggacat 841 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc 901 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg 961 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg 1021 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct 1081 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca 1141 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc 1201 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct 1261 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac 1321 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa 1381 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg 1441 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt 1501 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag 1561 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga 1621 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgaaagctt 1681 gggattcgaa tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 1741 agacataata gcaacagaca tacaaactaa agaactacaa aaacaaatta caaaaattca 1801 aaattttcgg gtttttcgaa cctagggttc cgcgttacat aacttacggt aaatggcccg 1861 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata 1921 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc 1981 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac 2041 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg 2101 cagtacatct acgtttagtc atcgctatta ccatggtgat gcggttttgg cagtacatca 2161 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca 2221 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg 2281 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc 2341 gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa 2401 gaaccgagtt taaactccct atcagtgata gagatctccc tatcagtgat agagagctag 2461 ccccgggatc gatcaattgc cggtcgccac catggtgagc aagggcgagg agctgttcac 2521 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt 2581 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac 2641 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca 2701 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc 2761 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg 2821 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga 2881 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa 2941 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca 3001 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg 3061 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa 3121 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat 3181 cactctcggc atggacgagc tgtacaagta aagtacgtag gtaccccagt gtggtggcct 3241 gcaggtgaat tcactagtac cggtaggcct gtcgacgata tcgggcccgc ggccgctgga 3301 tcctctagac tgcagctcga gtacccatac gacgtcccag actacgcttg agtttaaaca 3361 cgcgtggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct 3421 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca 3481 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc 3541 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta 3601 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt 3661 tggaggccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtccctc 3721 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtgg 3781 acccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg 3841 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca 3901 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt 3961 tgagcggttc ccggctggcc gcgcagcaac agatggaagg gctcctggcg ccgcaccggc 4021 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg 4081 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg 4141 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg 4201 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg 4261 gtgcctgaac gcgttccgga aatcaacctc tggattacaa aatttgtgaa agattgactg 4321 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt 4381 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc 4441 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt 4501 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga 4561 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct 4621 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga 4681 cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct 4741 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc 4801 tgcggcctct tccgcgtctc gccttcgccc tcagacgagt cggatctccc tttgggccgc 4861 ctccccgcct gtccggatgg aagggctaat tcactcccaa cgaatacaag atctgctttt 4921 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact 4981 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc 5041 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa 5101 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa 5161 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag 5221 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt 5281 gtccaaactc atcaatgtat cttatcatgt ctggcatcta tgtcgggtgc ggagaaagag 5341 gtaatgaaat ggcattatgg gtattatggg tctgcattaa tgaatcggcc aacggatgtc 5401 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt 5461 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact 5521 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag 5581 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata 5641 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 5701 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 5761 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc 5821 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 5881 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc 5941 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga 6001 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg 6061 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 6121 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg 6181 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt 6241 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gccatgagat 6301 tatcaaaaag gatcttcacc tagatccttt tcacgtagaa agccagtccg cagaaacggt 6361 gctgaccccg gatgaatgtc agctactggg ctatctggac aagggaaaac gcaagcgcaa 6421 agagaaagca ggtagcttgc agtgggctta catggcgata gctagactgg gcggttttat 6481 ggacagcaag cgaaccggaa ttgccagctg gggcgccctc tggtaaggtt gggaagccct 6541 gcaaagtaaa ctggatggct ttcttgccgc caaggatctg atggcgcagg ggatcaagct 6601 ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag 6661 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg 6721 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca 6781 agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc 6841 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg 6901 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg 6961 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta 7021 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag 7081 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac 7141 tgttcgccag gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg 7201 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg 7261 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg 7321 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg 7381 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgaatt ttgttaaaat 7441 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa 7501 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta 7561 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca 7621 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat 7681 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg 7741 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc 7801 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtccatt 7861 cgccattcag gatcgaatta attcttaatt aacatcatca ataatatacc ttttttggat 7921 tgaagccaat atgataatga gggggtggag tttgtgacgt ggcgcggggc gtgggaacgg 7981 ggcgggtgac gtagtagtgt ggcggaagtg tgatgttgca agtgtggcgg aacacatgta 8041 agcgacggat gtggcaaaag tgacgttttt ggtgtgcgcc ggtgtacaca ggaagtgaca 8101 attttcgcgc ggttttaggc ggatgttgta gtaaatttgg gcgtaaccga gtaagatttg 8161 gccattttcg cgggaaaact gaataagagg aagtgaaatc tgaataattt tgtgttactc 8221 atagcgcgta atactctcat gagcggatac atatttgaat gtatttagaa aaataaacaa 8281 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtaactat aacggtccta 8341 aggtagcgaa aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga 8401 gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa 8461 ggtggtacga t
FEATURES Location/Qualifiers source 1..8471 /organism="recombinant plasmid" /mol_type="other DNA" LTR 109..289 /note="5' LTR" /note="truncated 5' long terminal repeat (LTR) from HIV-1" /note="color: #ffe4c4" misc_feature 336..461 /note="HIV-1 psi" /note="packaging signal of human immunodeficiency virus type 1" /note="color: #ffe4c4" misc_feature 951..1184 /note="HIV-1 RRE" /note="The Rev response element (RRE) of human immunodeficiency virus type 1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /note="color: #ffe4c4" enhancer 1831..2135 /note="CMV enhancer" /note="human cytomegalovirus immediate early enhancer" /note="color: #ffffff" promoter 2136..2339 /note="CMV promoter" /note="human cytomegalovirus (CMV) immediate early promoter" /note="color: #ffffff; direction: RIGHT" protein_bind 2416..2434 /gene="tetO" /bound_moiety="tetracycline repressor TetR" /note="tet operator" /note=" " /note="color: #31849b" protein_bind 2437..2455 /gene="tetO" /bound_moiety="tetracycline repressor TetR" /note="tet operator" /note=" " /note="color: #31849b" CDS 2492..3211 /codon_start=1 /product="enhanced GFP" /note="EGFP" /note="mammalian codon-optimized" /note="This feature has 3 segments: 1: 2492 .. 2494 / #05fd14 2: 2495 .. 2497 / #05fd14 / 1a 3: 2498 .. 3211 / #05fd14" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 3322..3348 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /note="HA" /note="color: #cc99b2" /translation="YPYDVPDYA" rep_origin 3533..3668 /note="SV40 ori" /note="SV40 origin of replication" /note="color: #ffff00" CDS 3669..4268 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /note="PuroR" /note="confers resistance to puromycin" /note="color: #ccffcc" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" misc_feature 4281..4868 /note="WPRE" /note="woodchuck posttranscriptional regulatory element" /note="color: #ffffff" CDS complement(4752..4763) /codon_start=1 /product="Factor Xa recognition and cleavage site" /note="Factor Xa site" /note="color: #cc99b2" /translation="IEGR" misc_feature 4878..5111 /note="3'LTR (Delta-U3)" /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /note="color: #ffe4c4" LTR 4931..5111 /note="5' LTR" /note="truncated 5' long terminal repeat (LTR) from HIV-1" /note="color: #ffe4c4" polyA_signal 5183..5304 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" /note="color: #a6acb3" rep_origin complement(5633..6221) /direction=LEFT /note="ori" /note="high-copy-number colE1/pMB1/pBR322/pUC origin of replication" /note="color: #ffff00" CDS 6633..7427 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /note="NeoR/KanR" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin)" /note="color: #ccffcc" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"