pLenti-UTR-Luc

                            FEATURES             Location/Qualifiers
     source          1..9385
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     LTR             109..289
                     /note="5' LTR"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    336..461
                     /note="HIV-1 psi"
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    951..1184
                     /note="HIV-1 RRE"
                     /note="The Rev response element (RRE) of human 
                     immunodeficiency virus type 1 allows for Rev-dependent mRNA
                     export from the nucleus to the cytoplasm."
                     /note="color: #ffe4c4"
     enhancer        1831..2135
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        2136..2339
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     protein_bind    2416..2434
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="
                     "
                     /note="color: #31849b"
     protein_bind    2437..2455
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="
                     "
                     /note="color: #31849b"
     CDS             4236..4262
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /note="HA"
                     /note="color: #cc99b2"
                     /translation="YPYDVPDYA"
     rep_origin      4447..4582
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     CDS             4583..5182
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /note="PuroR"
                     /note="confers resistance to puromycin"
                     /note="color: #ccffcc"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    5195..5782
                     /note="WPRE"
                     /note="woodchuck posttranscriptional regulatory element"
                     /note="color: #ffffff"
     CDS             complement(5666..5677)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /note="Factor Xa site"
                     /note="color: #cc99b2"
                     /translation="IEGR"
     misc_feature    5792..6025
                     /note="3'LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     LTR             5845..6025
                     /note="5' LTR"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     polyA_signal    6097..6218
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      complement(6547..7135)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number colE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             7547..8341
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /note="NeoR/KanR"
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin)"
                     /note="color: #ccffcc"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
       1 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
       61 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
      121 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
      181 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
      241 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
      301 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
      361 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
      421 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
      481 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
      541 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
      601 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
      661 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
      721 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
      781 agaccaccgc acagcaagcc cgctgatctt cagacctgga ggaggagata tgagggacat
      841 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
      901 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
      961 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     1021 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     1081 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     1141 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     1201 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     1261 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     1321 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     1381 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     1441 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     1501 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     1561 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     1621 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgaaagctt
     1681 gggattcgaa tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     1741 agacataata gcaacagaca tacaaactaa agaactacaa aaacaaatta caaaaattca
     1801 aaattttcgg gtttttcgaa cctagggttc cgcgttacat aacttacggt aaatggcccg
     1861 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     1921 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     1981 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
     2041 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
     2101 cagtacatct acgtttagtc atcgctatta ccatggtgat gcggttttgg cagtacatca
     2161 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
     2221 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg
     2281 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc
     2341 gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa
     2401 gaaccgagtt taaactccct atcagtgata gagatctccc tatcagtgat agagagctag
     2461 agctagcagc caccatggaa gatgccaaaa acattaagaa gggcccagcg ccattctacc
     2521 cactcgaaga cgggaccgcc ggcgagcagc tgcacaaagc catgaagcgc tacgccctgg
     2581 tgcccggcac catcgccttt accgacgcac atatcgaggt ggacattacc tacgccgagt
     2641 acttcgagat gagcgttcgg ctggcagaag ctatgaagcg ctatgggctg aatacaaacc
     2701 atcggatcgt ggtgtgcagc gagaatagct tgcagttctt catgcccgtg ttgggtgccc
     2761 tgttcatcgg tgtggctgtg gccccagcta acgacatcta caacgagcgc gagctgctga
     2821 acagcatggg catcagccag cccaccgtcg tattcgtgag caagaaaggg ctgcaaaaga
     2881 tcctcaacgt gcaaaagaag ctaccgatca tacaaaagat catcatcatg gatagcaaga
     2941 ccgactacca gggcttccaa agcatgtaca ccttcgtgac ttcccatttg ccacccggct
     3001 tcaacgagta cgacttcgtg cccgagagct tcgaccggga caaaaccatc gccctgatca
     3061 tgaacagtag tggcagtacc ggattgccca agggcgtagc cctaccgcac cgcaccgctt
     3121 gtgtccgatt cagtcatgcc cgcgacccca tcttcggcaa ccagatcatc cccgacaccg
     3181 ctatcctcag cgtggtgcca tttcaccacg gcttcggcat gttcaccacg ctgggctact
     3241 tgatctgcgg ctttcgggtc gtgctcatgt accgcttcga ggaggagcta ttcttgcgca
     3301 gcttgcaaga ctataagatt caatctgccc tgctggtgcc cacactattt agcttcttcg
     3361 ctaagagcac tctcatcgac aagtacgacc taagcaactt gcacgagatc gccagcggcg
     3421 gggcgccgct cagcaaggag gtaggtgagg ccgtggccaa acgcttccac ctaccaggca
     3481 tccgccaggg ctacggcctg acagaaacaa ccagcgccat tctgatcacc cccgaagggg
     3541 acgacaagcc tggcgcagta ggcaaggtgg tgcccttctt cgaggctaag gtggtggact
     3601 tggacaccgg taagacactg ggtgtgaacc agcgcggcga gctgtgcgtc cgtggcccca
     3661 tgatcatgag cggctacgtt aacaaccccg aggctacaaa cgctctcatc gacaaggacg
     3721 gctggctgca cagcggcgac atcgcctact gggacgagga cgagcacttc ttcatcgtgg
     3781 accggctgaa gagcctgatc aaatacaagg gctaccaggt agccccagcc gaactggaga
     3841 gcatcctgct gcaacacccc aacatcttcg acgccggggt cgccggcctg cccgacgacg
     3901 atgccggcga gctgcccgcc gcagtcgtcg tgctggaaca cggtaaaacc atgaccgaga
     3961 aggagatcgt ggactatgtg gccagccagg ttacaaccgc caagaagctg cgcggtggtg
     4021 ttgtgttcgt ggacgaggtg cctaaaggac tgaccggcaa gttggacgcc cgcaagatcc
     4081 gcgagattct cattaaggcc aagaagggcg gcaagatcgc cgtgcaattg taaggtaccc
     4141 cagtgtggtg gcctgcaggt gaattcacta gtaccggtag gcctgtcgac gatatcgggc
     4201 ccgcggccgc tggatcctct agactgcagc tcgagtaccc atacgacgtc ccagactacg
     4261 cttgagttta aacacgcgtg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     4321 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     4381 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     4441 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     4501 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     4561 tgaggaggct tttttggagg ccatgaccga gtacaagccc acggtgcgcc tcgccacccg
     4621 cgacgacgtc cctcgggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
     4681 gcgccacacc gtggacccgg accgccacat cgagcgggtc accgagctgc aagaactctt
     4741 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt
     4801 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
     4861 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aagggctcct
     4921 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga
     4981 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
     5041 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
     5101 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
     5161 gacccgcaag cccggtgcct gaacgcgttc cggaaatcaa cctctggatt acaaaatttg
     5221 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     5281 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     5341 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     5401 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     5461 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     5521 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     5581 gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg
     5641 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     5701 cctgctgccg gctctgcggc ctcttccgcg tctcgccttc gccctcagac gagtcggatc
     5761 tccctttggg ccgcctcccc gcctgtccgg atggaagggc taattcactc ccaacgaata
     5821 caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     5881 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     5941 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     6001 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta
     6061 taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat
     6121 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     6181 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggca tctatgtcgg
     6241 gtgcggagaa agaggtaatg aaatggcatt atgggtatta tgggtctgca ttaatgaatc
     6301 ggccaacgga tgtcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
     6361 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     6421 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     6481 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     6541 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     6601 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     6661 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     6721 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     6781 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     6841 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     6901 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     6961 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     7021 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     7081 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     7141 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     7201 tttggccatg agattatcaa aaaggatctt cacctagatc cttttcacgt agaaagccag
     7261 tccgcagaaa cggtgctgac cccggatgaa tgtcagctac tgggctatct ggacaaggga
     7321 aaacgcaagc gcaaagagaa agcaggtagc ttgcagtggg cttacatggc gatagctaga
     7381 ctgggcggtt ttatggacag caagcgaacc ggaattgcca gctggggcgc cctctggtaa
     7441 ggttgggaag ccctgcaaag taaactggat ggctttcttg ccgccaagga tctgatggcg
     7501 caggggatca agctctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga
     7561 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
     7621 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
     7681 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc
     7741 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
     7801 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
     7861 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
     7921 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
     7981 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
     8041 cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt
     8101 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
     8161 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
     8221 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
     8281 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
     8341 aattttgtta aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
     8401 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
     8461 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
     8521 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
     8581 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
     8641 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
     8701 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
     8761 agggcgcgtc cattcgccat tcaggatcga attaattctt aattaacatc atcaataata
     8821 tacctttttt ggattgaagc caatatgata atgagggggt ggagtttgtg acgtggcgcg
     8881 gggcgtggga acggggcggg tgacgtagta gtgtggcgga agtgtgatgt tgcaagtgtg
     8941 gcggaacaca tgtaagcgac ggatgtggca aaagtgacgt ttttggtgtg cgccggtgta
     9001 cacaggaagt gacaattttc gcgcggtttt aggcggatgt tgtagtaaat ttgggcgtaa
     9061 ccgagtaaga tttggccatt ttcgcgggaa aactgaataa gaggaagtga aatctgaata
     9121 attttgtgtt actcatagcg cgtaatactc tcatgagcgg atacatattt gaatgtattt
     9181 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtaa
     9241 ctataacggt cctaaggtag cgaaaatgta gtcttatgca atactcttgt agtcttgcaa
     9301 catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg
     9361 attggtggaa gtaaggtggt acgat
                        

                            FEATURES             Location/Qualifiers
     source          1..9385
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     LTR             109..289
                     /note="5' LTR"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    336..461
                     /note="HIV-1 psi"
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    951..1184
                     /note="HIV-1 RRE"
                     /note="The Rev response element (RRE) of human 
                     immunodeficiency virus type 1 allows for Rev-dependent mRNA
                     export from the nucleus to the cytoplasm."
                     /note="color: #ffe4c4"
     enhancer        1831..2135
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        2136..2339
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     protein_bind    2416..2434
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="
                     "
                     /note="color: #31849b"
     protein_bind    2437..2455
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="
                     "
                     /note="color: #31849b"
     CDS             4236..4262
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /note="HA"
                     /note="color: #cc99b2"
                     /translation="YPYDVPDYA"
     rep_origin      4447..4582
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     CDS             4583..5182
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /note="PuroR"
                     /note="confers resistance to puromycin"
                     /note="color: #ccffcc"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    5195..5782
                     /note="WPRE"
                     /note="woodchuck posttranscriptional regulatory element"
                     /note="color: #ffffff"
     CDS             complement(5666..5677)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /note="Factor Xa site"
                     /note="color: #cc99b2"
                     /translation="IEGR"
     misc_feature    5792..6025
                     /note="3'LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     LTR             5845..6025
                     /note="5' LTR"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     polyA_signal    6097..6218
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      complement(6547..7135)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number colE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             7547..8341
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /note="NeoR/KanR"
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin)"
                     /note="color: #ccffcc"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"