pPB-N-His-GST
| Vector Name | pPB-N-His-GST |
| VectorType | Protein Vector |
| Antibiotic Information | Bacterial: Kanamycin. |
| Sequencing Primers | GST Forward primer: 5'-CACGTTTGGTGGTGGCGAC3' T7 terminator primer: 5'-GCTAGTTATTGCTCAGCGG-3' |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
ORIGIN
1 TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG
51 TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT
101 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG
151 TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC
201 GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG
251 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT
301 CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT
351 CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG
401 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT
451 ATTAACGTTT ACAATTTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA
501 CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG
551 AATTAATTCT TAGAAAAACT CATCGAGCAT CAAATGAAAC TGCAATTTAT
601 TCATATCAGG ATTATCAATA CCATATTTTT GAAAAAGCCG TTTCTGTAAT
651 GAAGGAGAAA ACTCACCGAG GCAGTTCCAT AGGATGGCAA GATCCTGGTA
701 TCGGTCTGCG ATTCCGACTC GTCCAACATC AATACAACCT ATTAATTTCC
751 CCTCGTCAAA AATAAGGTTA TCAAGTGAGA AATCACCATG AGTGACGACT
801 GAATCCGGTG AGAATGGCAA AAGTTTATGC ATTTCTTTCC AGACTTGTTC
851 AACAGGCCAG CCATTACGCT CGTCATCAAA ATCACTCGCA TCAACCAAAC
901 CGTTATTCAT TCGTGATTGC GCCTGAGCGA GACGAAATAC GCGATCGCTG
951 TTAAAAGGAC AATTACAAAC AGGAATCGAA TGCAACCGGC GCAGGAACAC
1001 TGCCAGCGCA TCAACAATAT TTTCACCTGA ATCAGGATAT TCTTCTAATA
1051 CCTGGAATGC TGTTTTCCCG GGGATCGCAG TGGTGAGTAA CCATGCATCA
1101 TCAGGAGTAC GGATAAAATG CTTGATGGTC GGAAGAGGCA TAAATTCCGT
1151 CAGCCAGTTT AGTCTGACCA TCTCATCTGT AACATCATTG GCAACGCTAC
1201 CTTTGCCATG TTTCAGAAAC AACTCTGGCG CATCGGGCTT CCCATACAAT
1251 CGATAGATTG TCGCACCTGA TTGCCCGACA TTATCGCGAG CCCATTTATA
1301 CCCATATAAA TCAGCATCCA TGTTGGAATT TAATCGCGGC CTAGAGCAAG
1351 ACGTTTCCCG TTGAATATGG CTCATAACAC CCCTTGTATT ACTGTTTATG
1401 TAAGCAGACA GTTTTATTGT TCATGACCAA AATCCCTTAA CGTGAGTTTT
1451 CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA
1501 GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC
1551 GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC
1601 CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA
1651 GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC
1701 ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA
1751 AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG
1801 CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG
1851 AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG
1901 CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG
1951 GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA
2001 TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT
2051 TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG
2101 GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT
2151 TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT
2201 GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG
2251 AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT
2301 GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC
2351 TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG
2401 GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC
2451 GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC
2501 GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG
2551 GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT
2601 CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT
2651 GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT
2701 GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA
2751 TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA
2801 CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT
2851 GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA
2901 ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG
2951 ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA
3001 CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG
3051 TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC
3101 TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG
3151 GAGCACGATC ATGCGCACCC GTGGGGCCGC CATGCCGGCG ATAATGGCCT
3201 GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG
3251 AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC
3301 GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA
3351 CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG
3401 ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT
3451 CAAGGGCATC GGTCGAGATC CCGGTGCCTA ATGAGTGAGC TAACTTACAT
3501 TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC
3551 CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT
3601 TGGGCGCCAG GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA
3651 TTGCCCTTCA CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT
3701 GGTTTGCCCC AGCAGGCGAA AATCCTGTTT GATGGTGGTT AACGGCGGGA
3751 TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC CGAGATATCC
3801 GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC
3851 CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA
3901 GCATTTGCAT GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC
3951 CGTTCCGCTA TCGGCTGAAT TTGATTGCGA GTGAGATATT TATGCCAGCC
4001 AGCCAGACGC AGACGCGCCG AGACAGAACT TAATGGGCCC GCTAACAGCG
4051 CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC CAGTCGCGTA
4101 CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC
4151 ATCAAGAAAT AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG
4201 CATCCTGGTC ATCCAGCGGA TAGTTAATGA TCAGCCCACT GACGCGTTGC
4251 GCGAGAAGAT TGTGCACCGC CGCTTTACAG GCTTCGACGC CGCTTCGTTC
4301 TACCATCGAC ACCACCACGC TGGCACCCAG TTGATCGGCG CGAGATTTAA
4351 TCGCCGCGAC AATTTGCGAC GGCGCGTGCA GGGCCAGACT GGAGGTGGCA
4401 ACGCCAATCA GCAACGACTG TTTGCCCGCC AGTTGTTGTG CCACGCGGTT
4451 GGGAATGTAA TTCAGCTCCG CCATCGCCGC TTCCACTTTT TCCCGCGTTT
4501 TCGCAGAAAC GTGGCTGGCC TGGTTCACCA CGCGGGAAAC GGTCTGATAA
4551 GAGACACCGG CATACTCTGC GACATCGTAT AACGTTACTG GTTTCACATT
4601 CACCACCCTG AATTGACTCT CTTCCGGGCG CTATCATGCC ATACCGCGAA
4651 AGGTTTTGCG CCATTCGATG GTGTCCGGGA TCTCGACGCT CTCCCTTATG
4701 CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA
4751 CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC
4801 CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG
4851 CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG
4901 CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG
4951 GCGTAGAGGA TCGAGATCTC GATCCCGCGA AATTAATACG ACTCACTATA
5001 GGGGAATTGT GAGCGGATAA CAATTCCCCT CTAGAAATAA TTTTGTTTAA
5051 CTTTAAGAAG GAGATATACC ATGGGCAGCA GCCATCATCA TCATCATCAC
5101 GGTACCTCCC CTATACTAGG TTATTGGAAA ATTAAGGGCC TTGTGCAACC
5151 CACTCGACTT CTTTTGGAAT ATCTTGAAGA AAAATATGAA GAGCATTTGT
5201 ATGAGCGCGA TGAAGGTGAT AAATGGCGAA ACAAAAAGTT TGAATTGGGT
5251 TTGGAGTTTC CCAATCTTCC TTATTATATT GATGGTGATG TTAAATTAAC
5301 ACAGTCTATG GCCATCATAC GTTATATAGC TGACAAGCAC AACATGTTGG
5351 GTGGTTGTCC AAAAGAGCGT GCAGAGATTT CAATGCTTGA AGGAGCGGTT
5401 TTGGATATTA GATACGGTGT TTCGAGAATT GCATATAGTA AAGACTTTGA
5451 AACTCTCAAA GTTGATTTTC TTAGCAAGCT ACCTGAAATG CTGAAAATGT
5501 TCGAAGATCG TTTATGTCAT AAAACATATT TAAATGGTGA TCATGTAACC
5551 CATCCTGACT TCATGTTGTA TGACGCTCTT GATGTTGTTT TATACATGGA
5601 CCCAATGTGC CTGGATGCGT TCCCAAAATT AGTTTGTTTT AAAAAACGTA
5651 TTGAAGCTAT CCCACAAATT GATAAGTACT TGAAATCCAG CAAGTATATA
5701 GCATGGCCTT TGCAGGGCTG GCAAGCCACG TTTGGTGGTG GCGACCATCC
5751 TCCAAAATCG GATGGTAGCG GCTCTGGTTC TGGTAGCGGC TCTGAAAACC
5801 TTTACTTCCA GGGCACTGCT AGCTACATCA CAAGTTTGTA CAAAAAAGCT
5851 GAACGAGAAA CGTAAAATGA TATAAATATC AATATATTAA ATTAGATTTT
5901 GCATAAAAAA CAGACTACAT AATACTGTAA AACACAACAT ATCCAGTCAC
5951 TATGGCGGCC GCATTAGGCA CCCCAGGCTT TACACTTTAT GCTTCCGGCT
6001 CGTATAATGT GTGGATTTTG AGTTAGGATC CGTCGAGATT TTCAGGAGCT
6051 AAGGAAGCTA AAATGGAGAA AAAAATCACT GGATATACCA CCGTTGATAT
6101 ATCCCAATGG CATCGTAAAG AACATTTTGA GGCATTTCAG TCAGTTGCTC
6151 AATGTACCTA TAACCAGACC GTTCAGCTGG ATATTACGGC CTTTTTAAAG
6201 ACCGTAAAGA AAAATAAGCA CAAGTTTTAT CCGGCCTTTA TTCACATTCT
6251 TGCCCGCCTG ATGAATGCTC ATCCGGAATT CCGTATGGCA ATGAAAGACG
6301 GTGAGCTGGT GATATGGGAT AGTGTTCACC CTTGTTACAC CGTTTTCCAT
6351 GAGCAAACTG AAACGTTTTC ATCGCTCTGG AGTGAATACC ACGACGATTT
6401 CCGGCAGTTT CTACACATAT ATTCGCAAGA TGTGGCGTGT TACGGTGAAA
6451 ACCTGGCCTA TTTCCCTAAA GGGTTTATTG AGAATATGTT TTTCGTCTCA
6501 GCCAATCCCT GGGTGAGTTT CACCAGTTTT GATTTAAACG TGGCCAATAT
6551 GGACAACTTC TTCGCCCCCG TTTTCACCAT GGGCAAATAT TATACGCAAG
6601 GCGACAAGGT GCTGATGCCG CTGGCGATTC AGGTTCATCA TGCCGTTTGT
6651 GATGGCTTCC ATGTCGGCAG AATGCTTAAT GAATTACAAC AGTACTGCGA
6701 TGAGTGGCAG GGCGGGGCGT AAAGATCTGG ATCCGGCTTA CTAAAAGCCA
6751 GATAACAGTA TGCGTATTTG CGCGCTGATT TTTGCGGTAT AAGAATATAT
6801 ACTGATATGT ATACCCGAAG TATGTCAAAA AGAGGTATGC TATGAAGCAG
6851 CGTATTACAG TGACAGTTGA CAGCGACAGC TATCAGTTGC TCAAGGCATA
6901 TATGATGTCA ATATCTCCGG TCTGGTAAGC ACAACCATGC AGAATGAAGC
6951 CCGTCGTCTG CGTGCCGAAC GCTGGAAAGC GGAAAATCAG GAAGGGATGG
7001 CTGAGGTCGC CCGGTTTATT GAAATGAACG GCTCTTTTGC TGACGAGAAC
7051 AGGGGCTGGT GAAATGCAGT TTAAGGTTTA CACCTATAAA AGAGAGAGCC
7101 GTTATCGTCT GTTTGTGGAT GTACAGAGTG ATATTATTGA CACGCCCGGG
7151 CGACGGATGG TGATCCCCCT GGCCAGTGCA CGTCTGCTGT CAGATAAAGT
7201 CTCCCGTGAA CTTTACCCGG TGGTGCATAT CGGGGATGAA AGCTGGCGCA
7251 TGATGACCAC CGATATGGCC AGTGTGCCGG TCTCCGTTAT CGGGGAAGAA
7301 GTGGCTGATC TCAGCCACCG CGAAAATGAC ATCAAAAACG CCATTAACCT
7351 GATGTTCTGG GGAATATAAA TGTCAGGCTC CCTTATACAC AGCCAGTCTG
7401 CAGGTCGACC ATAGTGACTG GATATGTTGT GTTTTACAGT ATTATGTAGT
7451 CTGTTTTTTA TGCAAAATCT AATTTAATAT ATTGATATTT ATATCATTTT
7501 ACGTTTCTCG TTCAGCTTTC TTGTACAAAG TGGTTGATAT CTGACTCGAG
7551 CACCACCACC ACCACCACTG AGATCCGGCT GCTAACAAAG CCCGAAAGGA
7601 AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG
7651 GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA
7701 TCCGGAT
//
LOCUS dna 7708 bp
FEATURES Location/Qualifiers
Rep_Origin 29..335
/gene="f1 origin"
Marker 560..1375
/gene="kan2 marker"
ORF 560..1375
/sequence="ORF_4 rf(4)"
misc_binding 1249..1254
/dbxref="REBASE:ClaI"
misc_binding 1284..1289
/dbxref="REBASE:NruI"
Rep_Origin 1481..2100
/gene="pBR322 origin"
Other Gene 2515..2706
/gene="ROP other"
misc_binding 3179..3189
/dbxref="REBASE:BglI"
Regulatory_Seq 3515..4606
/gene="lacI reg"
ORF 3515..4474
/sequence="ORF_3 rf(4)"
misc_binding 3738..3743
/dbxref="REBASE:HpaI"
misc_binding 4035..4040
/dbxref="REBASE:ApaI"
misc_binding 4771..4776
/dbxref="REBASE:SphI"
Promoter 4984..5002
/gene="T7 prom"
Regulatory_Seq 5002..5029
/gene="lacO reg"
misc_binding 5030..5035
/dbxref="REBASE:XbaI"
ORF 5071..5853
/sequence="ORF_1 rf(1)"
misc_binding 5101..5106
/dbxref="REBASE:KpnI"
Other Gene 5831..5955
/gene="attR1 other"
Other Gene 5855..5955
/gene="attR2 other"
misc_binding 5956..5963
/dbxref="REBASE:NotI"
Marker 6064..6723
/gene="CAT marker"
ORF 6064..6723
/sequence="ORF_2 rf(1)"
misc_binding 6277..6282
/dbxref="REBASE:EcoRI"
Other Gene 7065..7370
/gene="ccdB other"
misc_binding 7399..7404
/dbxref="REBASE:PstI"
Other Gene 7411..7530
/gene="attR1 other"
Other Gene 7411..7511
/gene="attR2 other"
misc_binding 7546..7551
/dbxref="REBASE:XhoI"
Terminator 7580..7708
/gene="T7 term"
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
f1 origin(29,335)>>>
|
1 tggcgaatgggacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcg 60
ACCGCTTACCCTGCGCGGGACATCGCCGCGTAATTCGCGCCGCCCACACCACCAATGCGC
61 cagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttc 120
GTCGCACTGGCGATGTGAACGGTCGCGGGATCGCGGGCGAGGAAAGCGAAAGAAGGGAAG
121 ctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagg 180
GAAAGAGCGGTGCAAGCGGCCGAAAGGGGCAGTTCGAGATTTAGCCCCCGAGGGAAATCC
181 gttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttc 240
CAAGGCTAAATCACGAAATGCCGTGGAGCTGGGGTTTTTTGAACTAATCCCACTACCAAG
241 acgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgtt 300
TGCATCACCCGGTAGCGGGACTATCTGCCAAAAAGCGGGAAACTGCAACCTCAGGTGCAA
301 ctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattc 360
GAAATTATCACCTGAGAACAAGGTTTGACCTTGTTGTGAGTTGGGATAGAGCCAGATAAG
361 ttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgattta 420
AAAACTAAATATTCCCTAAAACGGCTAAAGCCGGATAACCAATTTTTTACTCGACTAAAT
421 acaaaaatttaacgcgaattttaacaaaatattaacgtttacaatttcaggtggcacttt 480
TGTTTTTAAATTGCGCTTAAAATTGTTTTATAATTGCAAATGTTAAAGTCCACCGTGAAA
481 tcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgta 540
AGCCCCTTTACACGCGCCTTGGGGATAAACAAATAAAAAGATTTATGTAAGTTTATACAT
ORF_4 rf(4)(560,1375)<<<
|
kan2 marker(560,1375)<<<
|
541 tccgctcatgaattaattcttagaaaaactcatcgagcatcaaatgaaactgcaatttat 600
AGGCGAGTACTTAATTAAGAATCTTTTTGAGTAGCTCGTAGTTTACTTTGACGTTAAATA
601 tcatatcaggattatcaataccatatttttgaaaaagccgtttctgtaatgaaggagaaa 660
AGTATAGTCCTAATAGTTATGGTATAAAAACTTTTTCGGCAAAGACATTACTTCCTCTTT
661 actcaccgaggcagttccataggatggcaagatcctggtatcggtctgcgattccgactc 720
TGAGTGGCTCCGTCAAGGTATCCTACCGTTCTAGGACCATAGCCAGACGCTAAGGCTGAG
721 gtccaacatcaatacaacctattaatttcccctcgtcaaaaataaggttatcaagtgaga 780
CAGGTTGTAGTTATGTTGGATAATTAAAGGGGAGCAGTTTTTATTCCAATAGTTCACTCT
781 aatcaccatgagtgacgactgaatccggtgagaatggcaaaagtttatgcatttctttcc 840
TTAGTGGTACTCACTGCTGACTTAGGCCACTCTTACCGTTTTCAAATACGTAAAGAAAGG
841 agacttgttcaacaggccagccattacgctcgtcatcaaaatcactcgcatcaaccaaac 900
TCTGAACAAGTTGTCCGGTCGGTAATGCGAGCAGTAGTTTTAGTGAGCGTAGTTGGTTTG
901 cgttattcattcgtgattgcgcctgagcgagacgaaatacgcgatcgctgttaaaaggac 960
GCAATAAGTAAGCACTAACGCGGACTCGCTCTGCTTTATGCGCTAGCGACAATTTTCCTG
961 aattacaaacaggaatcgaatgcaaccggcgcaggaacactgccagcgcatcaacaatat 1020
TTAATGTTTGTCCTTAGCTTACGTTGGCCGCGTCCTTGTGACGGTCGCGTAGTTGTTATA
1021 tttcacctgaatcaggatattcttctaatacctggaatgctgttttcccggggatcgcag 1080
AAAGTGGACTTAGTCCTATAAGAAGATTATGGACCTTACGACAAAAGGGCCCCTAGCGTC
1081 tggtgagtaaccatgcatcatcaggagtacggataaaatgcttgatggtcggaagaggca 1140
ACCACTCATTGGTACGTAGTAGTCCTCATGCCTATTTTACGAACTACCAGCCTTCTCCGT
1141 taaattccgtcagccagtttagtctgaccatctcatctgtaacatcattggcaacgctac 1200
ATTTAAGGCAGTCGGTCAAATCAGACTGGTAGAGTAGACATTGTAGTAACCGTTGCGATG
ClaI
|
1201 ctttgccatgtttcagaaacaactctggcgcatcgggcttcccatacaatcgatagattg 1260
GAAACGGTACAAAGTCTTTGTTGAGACCGCGTAGCCCGAAGGGTATGTTAGCTATCTAAC
NruI
|
1261 tcgcacctgattgcccgacattatcgcgagcccatttatacccatataaatcagcatcca 1320
AGCGTGGACTAACGGGCTGTAATAGCGCTCGGGTAAATATGGGTATATTTAGTCGTAGGT
1321 tgttggaatttaatcgcggcctagagcaagacgtttcccgttgaatatggctcataacac 1380
ACAACCTTAAATTAGCGCCGGATCTCGTTCTGCAAAGGGCAACTTATACCGAGTATTGTG
1381 cccttgtattactgtttatgtaagcagacagttttattgttcatgaccaaaatcccttaa 1440
GGGAACATAATGACAAATACATTCGTCTGTCAAAATAACAAGTACTGGTTTTAGGGAATT
pBR322 origin(1481,2100)>>>
|
1441 cgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttga 1500
GCACTCAAAAGCAAGGTGACTCGCAGTCTGGGGCATCTTTTCTAGTTTCCTAGAAGAACT
1501 gatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcg 1560
CTAGGAAAAAAAGACGCGCATTAGACGACGAACGTTTGTTTTTTTGGTGGCGATGGTCGC
1561 gtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagc 1620
CACCAAACAAACGGCCTAGTTCTCGATGGTTGAGAAAAAGGCTTCCATTGACCGAAGTCG
1621 agagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaag 1680
TCTCGCGTCTATGGTTTATGACAGGAAGATCACATCGGCATCAATCCGGTGGTGAAGTTC
1681 aactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgcc 1740
TTGAGACATCGTGGCGGATGTATGGAGCGAGACGATTAGGACAATGGTCACCGACGACGG
1741 agtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcg 1800
TCACCGCTATTCAGCACAGAATGGCCCAACCTGAGTTCTGCTATCAATGGCCTATTCCGC
1801 cagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctac 1860
GTCGCCAGCCCGACTTGCCCCCCAAGCACGTGTGTCGGGTCGAACCTCGCTTGCTGGATG
1861 accgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggaga 1920
TGGCTTGACTCTATGGATGTCGCACTCGATACTCTTTCGCGGTGCGAAGGGCTTCCCTCT
1921 aaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagctt 1980
TTCCGCCTGTCCATAGGCCATTCGCCGTCCCAGCCTTGTCCTCTCGCGTGCTCCCTCGAA
1981 ccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgag 2040
GGTCCCCCTTTGCGGACCATAGAAATATCAGGACAGCCCAAAGCGGTGGAGACTGAACTC
2041 cgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcg 2100
GCAGCTAAAAACACTACGAGCAGTCCCCCCGCCTCGGATACCTTTTTGCGGTCGTTGCGC
2101 gcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgtta 2160
CGGAAAAATGCCAAGGACCGGAAAACGACCGGAAAACGAGTGTACAAGAAAGGACGCAAT
2161 tcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgc 2220
AGGGGACTAAGACACCTATTGGCATAATGGCGGAAACTCACTCGACTATGGCGAGCGGCG
2221 agccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcctgatgcgg 2280
TCGGCTTGCTGGCTCGCGTCGCTCAGTCACTCGCTCCTTCGCCTTCTCGCGGACTACGCC
2281 tattttctccttacgcatctgtgcggtatttcacaccgcatatatggtgcactctcagta 2340
ATAAAAGAGGAATGCGTAGACACGCCATAAAGTGTGGCGTATATACCACGTGAGAGTCAT
2341 caatctgctctgatgccgcatagttaagccagtatacactccgctatcgctacgtgactg 2400
GTTAGACGAGACTACGGCGTATCAATTCGGTCATATGTGAGGCGATAGCGATGCACTGAC
2401 ggtcatggctgcgccccgacacccgccaacacccgctgacgcgccctgacgggcttgtct 2460
CCAGTACCGACGCGGGGCTGTGGGCGGTTGTGGGCGACTGCGCGGGACTGCCCGAACAGA
ROP other(2515,2706)<<<
|
2461 gctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagag 2520
CGAGGGCCGTAGGCGAATGTCTGTTCGACACTGGCAGAGGCCCTCGACGTACACAGTCTC
2521 gttttcaccgtcatcaccgaaacgcgcgaggcagctgcggtaaagctcatcagcgtggtc 2580
CAAAAGTGGCAGTAGTGGCTTTGCGCGCTCCGTCGACGCCATTTCGAGTAGTCGCACCAG
2581 gtgaagcgattcacagatgtctgcctgttcatccgcgtccagctcgttgagtttctccag 2640
CACTTCGCTAAGTGTCTACAGACGGACAAGTAGGCGCAGGTCGAGCAACTCAAAGAGGTC
2641 aagcgttaatgtctggcttctgataaagcgggccatgttaagggcggttttttcctgttt 2700
TTCGCAATTACAGACCGAAGACTATTTCGCCCGGTACAATTCCCGCCAAAAAAGGACAAA
2701 ggtcactgatgcctccgtgtaagggggatttctgttcatgggggtaatgataccgatgaa 2760
CCAGTGACTACGGAGGCACATTCCCCCTAAAGACAAGTACCCCCATTACTATGGCTACTT
2761 acgagagaggatgctcacgatacgggttactgatgatgaacatgcccggttactggaacg 2820
TGCTCTCTCCTACGAGTGCTATGCCCAATGACTACTACTTGTACGGGCCAATGACCTTGC
2821 ttgtgagggtaaacaactggcggtatggatgcggcgggaccagagaaaaatcactcaggg 2880
AACACTCCCATTTGTTGACCGCCATACCTACGCCGCCCTGGTCTCTTTTTAGTGAGTCCC
2881 tcaatgccagcgcttcgttaatacagatgtaggtgttccacagggtagccagcagcatcc 2940
AGTTACGGTCGCGAAGCAATTATGTCTACATCCACAAGGTGTCCCATCGGTCGTCGTAGG
2941 tgcgatgcagatccggaacataatggtgcagggcgctgacttccgcgtttccagacttta 3000
ACGCTACGTCTAGGCCTTGTATTACCACGTCCCGCGACTGAAGGCGCAAAGGTCTGAAAT
3001 cgaaacacggaaaccgaagaccattcatgttgttgctcaggtcgcagacgttttgcagca 3060
GCTTTGTGCCTTTGGCTTCTGGTAAGTACAACAACGAGTCCAGCGTCTGCAAAACGTCGT
3061 gcagtcgcttcacgttcgctcgcgtatcggtgattcattctgctaaccagtaaggcaacc 3120
CGTCAGCGAAGTGCAAGCGAGCGCATAGCCACTAAGTAAGACGATTGGTCATTCCGTTGG
3121 ccgccagcctagccgggtcctcaacgacaggagcacgatcatgcgcacccgtggggccgc 3180
GGCGGTCGGATCGGCCCAGGAGTTGCTGTCCTCGTGCTAGTACGCGTGGGCACCCCGGCG
BglI
|
3181 catgccggcgataatggcctgcttctcgccgaaacgtttggtggcgggaccagtgacgaa 3240
GTACGGCCGCTATTACCGGACGAAGAGCGGCTTTGCAAACCACCGCCCTGGTCACTGCTT
3241 ggcttgagcgagggcgtgcaagattccgaataccgcaagcgacaggccgatcatcgtcgc 3300
CCGAACTCGCTCCCGCACGTTCTAAGGCTTATGGCGTTCGCTGTCCGGCTAGTAGCAGCG
3301 gctccagcgaaagcggtcctcgccgaaaatgacccagagcgctgccggcacctgtcctac 3360
CGAGGTCGCTTTCGCCAGGAGCGGCTTTTACTGGGTCTCGCGACGGCCGTGGACAGGATG
3361 gagttgcatgataaagaagacagtcataagtgcggcgacgatagtcatgccccgcgccca 3420
CTCAACGTACTATTTCTTCTGTCAGTATTCACGCCGCTGCTATCAGTACGGGGCGCGGGT
3421 ccggaaggagctgactgggttgaaggctctcaagggcatcggtcgagatcccggtgccta 3480
GGCCTTCCTCGACTGACCCAACTTCCGAGAGTTCCCGTAGCCAGCTCTAGGGCCACGGAT
lacI reg(3515,4606)<<<
|
ORF_3 rf(4)(3515,4474)<<<
|
3481 atgagtgagctaacttacattaattgcgttgcgctcactgcccgctttccagtcgggaaa 3540
TACTCACTCGATTGAATGTAATTAACGCAACGCGAGTGACGGGCGAAAGGTCAGCCCTTT
3541 cctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtat 3600
GGACAGCACGGTCGACGTAATTACTTAGCCGGTTGCGCGCCCCTCTCCGCCAAACGCATA
3601 tgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttca 3660
ACCCGCGGTCCCACCAAAAAGAAAAGTGGTCACTCTGCCCGTTGTCGACTAACGGGAAGT
3661 ccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaa 3720
GGCGGACCGGGACTCTCTCAACGTCGTTCGCCAGGTGCGACCAAACGGGGTCGTCCGCTT
HpaI
|
3721 aatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgt 3780
TTAGGACAAACTACCACCAATTGCCGCCCTATATTGTACTCGACAGAAGCCATAGCAGCA
3781 atcccactaccgagatatccgcaccaacgcgcagcccggactcggtaatggcgcgcattg 3840
TAGGGTGATGGCTCTATAGGCGTGGTTGCGCGTCGGGCCTGAGCCATTACCGCGCGTAAC
3841 cgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattca 3900
GCGGGTCGCGGTAGACTAGCAACCGTTGGTCGTAGCGTCACCCTTGCTACGGGAGTAAGT
3901 gcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgcta 3960
CGTAAACGTACCAAACAACTTTTGGCCTGTACCGTGAGGTCAGCGGAAGGGCAAGGCGAT
3961 tcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccg 4020
AGCCGACTTAAACTAACGCTCACTCTATAAATACGGTCGGTCGGTCTGCGTCTGCGCGGC
ApaI
|
4021 agacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagat 4080
TCTGTCTTGAATTACCCGGGCGATTGTCGCGCTAAACGACCACTGGGTTACGCTGGTCTA
4081 gctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtct 4140
CGAGGTGCGGGTCAGCGCATGGCAGAAGTACCCTCTTTTATTATGACAACTACCCACAGA
4141 ggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatgg 4200
CCAGTCTCTGTAGTTCTTTATTGCGGCCTTGTAATCACGTCCGTCGAAGGTGTCGTTACC
4201 catcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagat 4260
GTAGGACCAGTAGGTCGCCTATCAATTACTAGTCGGGTGACTGCGCAACGCGCTCTTCTA
4261 tgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgc 4320
ACACGTGGCGGCGAAATGTCCGAAGCTGCGGCGAAGCAAGATGGTAGCTGTGGTGGTGCG
4321 tggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgca 4380
ACCGTGGGTCAACTAGCCGCGCTCTAAATTAGCGGCGCTGTTAAACGCTGCCGCGCACGT
4381 gggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtg 4440
CCCGGTCTGACCTCCACCGTTGCGGTTAGTCGTTGCTGACAAACGGGCGGTCAACAACAC
4441 ccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttt 4500
GGTGCGCCAACCCTTACATTAAGTCGAGGCGGTAGCGGCGAAGGTGAAAAAGGGCGCAAA
4501 tcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccgg 4560
AGCGTCTTTGCACCGACCGGACCAAGTGGTGCGCCCTTTGCCAGACTATTCTCTGTGGCC
4561 catactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactct 4620
GTATGAGACGCTGTAGCATATTGCAATGACCAAAGTGTAAGTGGTGGGACTTAACTGAGA
4621 cttccgggcgctatcatgccataccgcgaaaggttttgcgccattcgatggtgtccggga 4680
GAAGGCCCGCGATAGTACGGTATGGCGCTTTCCAAAACGCGGTAAGCTACCACAGGCCCT
4681 tctcgacgctctcccttatgcgactcctgcattaggaagcagcccagtagtaggttgagg 4740
AGAGCTGCGAGAGGGAATACGCTGAGGACGTAATCCTTCGTCGGGTCATCATCCAACTCC
SphI
|
4741 ccgttgagcaccgccgccgcaaggaatggtgcatgcaaggagatggcgcccaacagtccc 4800
GGCAACTCGTGGCGGCGGCGTTCCTTACCACGTACGTTCCTCTACCGCGGGTTGTCAGGG
4801 ccggccacggggcctgccaccatacccacgccgaaacaagcgctcatgagcccgaagtgg 4860
GGCCGGTGCCCCGGACGGTGGTATGGGTGCGGCTTTGTTCGCGAGTACTCGGGCTTCACC
4861 cgagcccgatcttccccatcggtgatgtcggcgatataggcgccagcaaccgcacctgtg 4920
GCTCGGGCTAGAAGGGGTAGCCACTACAGCCGCTATATCCGCGGTCGTTGGCGTGGACAC
4921 gcgccggtgatgccggccacgatgcgtccggcgtagaggatcgagatctcgatcccgcga 4980
CGCGGCCACTACGGCCGGTGCTACGCAGGCCGCATCTCCTAGCTCTAGAGCTAGGGCGCT
lacO reg(5002,5029)>>>
|
T7 prom(4984,5002)>>> XbaI
| | |
4981 aattaatacgactcactataggggaattgtgagcggataacaattcccctctagaaataa 5040
TTAATTATGCTGAGTGATATCCCCTTAACACTCGCCTATTGTTAAGGGGAGATCTTTATT
ORF_1 rf(1)(5071,5853)>>>
|
5041 ttttgtttaactttaagaaggagatataccatgggcagcagccatcatcatcatcatcac 5100
AAAACAAATTGAAATTCTTCCTCTATATGGTACCCGTCGTCGGTAGTAGTAGTAGTAGTG
KpnI
|
5101 ggtacctcccctatactaggttattggaaaattaagggccttgtgcaacccactcgactt 5160
CCATGGAGGGGATATGATCCAATAACCTTTTAATTCCCGGAACACGTTGGGTGAGCTGAA
5161 cttttggaatatcttgaagaaaaatatgaagagcatttgtatgagcgcgatgaaggtgat 5220
GAAAACCTTATAGAACTTCTTTTTATACTTCTCGTAAACATACTCGCGCTACTTCCACTA
5221 aaatggcgaaacaaaaagtttgaattgggtttggagtttcccaatcttccttattatatt 5280
TTTACCGCTTTGTTTTTCAAACTTAACCCAAACCTCAAAGGGTTAGAAGGAATAATATAA
5281 gatggtgatgttaaattaacacagtctatggccatcatacgttatatagctgacaagcac 5340
CTACCACTACAATTTAATTGTGTCAGATACCGGTAGTATGCAATATATCGACTGTTCGTG
5341 aacatgttgggtggttgtccaaaagagcgtgcagagatttcaatgcttgaaggagcggtt 5400
TTGTACAACCCACCAACAGGTTTTCTCGCACGTCTCTAAAGTTACGAACTTCCTCGCCAA
5401 ttggatattagatacggtgtttcgagaattgcatatagtaaagactttgaaactctcaaa 5460
AACCTATAATCTATGCCACAAAGCTCTTAACGTATATCATTTCTGAAACTTTGAGAGTTT
5461 gttgattttcttagcaagctacctgaaatgctgaaaatgttcgaagatcgtttatgtcat 5520
CAACTAAAAGAATCGTTCGATGGACTTTACGACTTTTACAAGCTTCTAGCAAATACAGTA
5521 aaaacatatttaaatggtgatcatgtaacccatcctgacttcatgttgtatgacgctctt 5580
TTTTGTATAAATTTACCACTAGTACATTGGGTAGGACTGAAGTACAACATACTGCGAGAA
5581 gatgttgttttatacatggacccaatgtgcctggatgcgttcccaaaattagtttgtttt 5640
CTACAACAAAATATGTACCTGGGTTACACGGACCTACGCAAGGGTTTTAATCAAACAAAA
5641 aaaaaacgtattgaagctatcccacaaattgataagtacttgaaatccagcaagtatata 5700
TTTTTTGCATAACTTCGATAGGGTGTTTAACTATTCATGAACTTTAGGTCGTTCATATAT
5701 gcatggcctttgcagggctggcaagccacgtttggtggtggcgaccatcctccaaaatcg 5760
CGTACCGGAAACGTCCCGACCGTTCGGTGCAAACCACCACCGCTGGTAGGAGGTTTTAGC
5761 gatggtagcggctctggttctggtagcggctctgaaaacctttacttccagggcactgct 5820
CTACCATCGCCGAGACCAAGACCATCGCCGAGACTTTTGGAAATGAAGGTCCCGTGACGA
attR2 other(5855,5955)<<<
|
attR1 other(5831,5955)>>>
| |
5821 agcgatatcaacaagtttgtacaaaaaagctgaacgagaaacgtaaaatgatataaatat 5880
TCGCTATAGTTGTTCAAACATGTTTTTTCGACTTGCTCTTTGCATTTTACTATATTTATA
5881 caatatattaaattagattttgcataaaaaacagactacataatactgtaaaacacaaca 5940
GTTATATAATTTAATCTAAAACGTATTTTTTGTCTGATGTATTATGACATTTTGTGTTGT
NotI
|
5941 tatccagtcactatggcggccgcattaggcaccccaggctttacactttatgcttccggc 6000
ATAGGTCAGTGATACCGCCGGCGTAATCCGTGGGGTCCGAAATGTGAAATACGAAGGCCG
6001 tcgtataatgtgtggattttgagttaggatccgtcgagattttcaggagctaaggaagct 6060
AGCATATTACACACCTAAAACTCAATCCTAGGCAGCTCTAAAAGTCCTCGATTCCTTCGA
ORF_2 rf(1)(6064,6723)>>>
|
CAT marker(6064,6723)>>>
|
6061 aaaatggagaaaaaaatcactggatataccaccgttgatatatcccaatggcatcgtaaa 6120
TTTTACCTCTTTTTTTAGTGACCTATATGGTGGCAACTATATAGGGTTACCGTAGCATTT
6121 gaacattttgaggcatttcagtcagttgctcaatgtacctataaccagaccgttcagctg 6180
CTTGTAAAACTCCGTAAAGTCAGTCAACGAGTTACATGGATATTGGTCTGGCAAGTCGAC
6181 gatattacggcctttttaaagaccgtaaagaaaaataagcacaagttttatccggccttt 6240
CTATAATGCCGGAAAAATTTCTGGCATTTCTTTTTATTCGTGTTCAAAATAGGCCGGAAA
EcoRI
|
6241 attcacattcttgcccgcctgatgaatgctcatccggaattccgtatggcaatgaaagac 6300
TAAGTGTAAGAACGGGCGGACTACTTACGAGTAGGCCTTAAGGCATACCGTTACTTTCTG
6301 ggtgagctggtgatatgggatagtgttcacccttgttacaccgttttccatgagcaaact 6360
CCACTCGACCACTATACCCTATCACAAGTGGGAACAATGTGGCAAAAGGTACTCGTTTGA
6361 gaaacgttttcatcgctctggagtgaataccacgacgatttccggcagtttctacacata 6420
CTTTGCAAAAGTAGCGAGACCTCACTTATGGTGCTGCTAAAGGCCGTCAAAGATGTGTAT
6421 tattcgcaagatgtggcgtgttacggtgaaaacctggcctatttccctaaagggtttatt 6480
ATAAGCGTTCTACACCGCACAATGCCACTTTTGGACCGGATAAAGGGATTTCCCAAATAA
6481 gagaatatgtttttcgtctcagccaatccctgggtgagtttcaccagttttgatttaaac 6540
CTCTTATACAAAAAGCAGAGTCGGTTAGGGACCCACTCAAAGTGGTCAAAACTAAATTTG
6541 gtggccaatatggacaacttcttcgcccccgttttcaccatgggcaaatattatacgcaa 6600
CACCGGTTATACCTGTTGAAGAAGCGGGGGCAAAAGTGGTACCCGTTTATAATATGCGTT
6601 ggcgacaaggtgctgatgccgctggcgattcaggttcatcatgccgtttgtgatggcttc 6660
CCGCTGTTCCACGACTACGGCGACCGCTAAGTCCAAGTAGTACGGCAAACACTACCGAAG
6661 catgtcggcagaatgcttaatgaattacaacagtactgcgatgagtggcagggcggggcg 6720
GTACAGCCGTCTTACGAATTACTTAATGTTGTCATGACGCTACTCACCGTCCCGCCCCGC
6721 taaagatctggatccggcttactaaaagccagataacagtatgcgtatttgcgcgctgat 6780
ATTTCTAGACCTAGGCCGAATGATTTTCGGTCTATTGTCATACGCATAAACGCGCGACTA
6781 ttttgcggtataagaatatatactgatatgtatacccgaagtatgtcaaaaagaggtatg 6840
AAAACGCCATATTCTTATATATGACTATACATATGGGCTTCATACAGTTTTTCTCCATAC
6841 ctatgaagcagcgtattacagtgacagttgacagcgacagctatcagttgctcaaggcat 6900
GATACTTCGTCGCATAATGTCACTGTCAACTGTCGCTGTCGATAGTCAACGAGTTCCGTA
6901 atatgatgtcaatatctccggtctggtaagcacaaccatgcagaatgaagcccgtcgtct 6960
TATACTACAGTTATAGAGGCCAGACCATTCGTGTTGGTACGTCTTACTTCGGGCAGCAGA
6961 gcgtgccgaacgctggaaagcggaaaatcaggaagggatggctgaggtcgcccggtttat 7020
CGCACGGCTTGCGACCTTTCGCCTTTTAGTCCTTCCCTACCGACTCCAGCGGGCCAAATA
ccdB other(7065,7370)>>>
|
7021 tgaaatgaacggctcttttgctgacgagaacaggggctggtgaaatgcagtttaaggttt 7080
ACTTTACTTGCCGAGAAAACGACTGCTCTTGTCCCCGACCACTTTACGTCAAATTCCAAA
7081 acacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattg 7140
TGTGGATATTTTCTCTCTCGGCAATAGCAGACAAACACCTACATGTCTCACTATAATAAC
7141 acacgcccgggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaag 7200
TGTGCGGGCCCGCTGCCTACCACTAGGGGGACCGGTCACGTGCAGACGACAGTCTATTTC
7201 tctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgacca 7260
AGAGGGCACTTGAAATGGGCCACCACGTATAGCCCCTACTTTCGACCGCGTACTACTGGT
7261 ccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccacc 7320
GGCTATACCGGTCACACGGCCAGAGGCAATAGCCCCTTCTTCACCGACTAGAGTCGGTGG
7321 gcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatataaatgtcaggct 7380
CGCTTTTACTGTAGTTTTTGCGGTAATTGGACTACAAGACCCCTTATATTTACAGTCCGA
attR2 other(7411,7511)>>>
|
PstI attR1 other(7411,7530)<<<
| |
7381 cccttatacacagccagtctgcaggtcgaccatagtgactggatatgttgtgttttacag 7440
GGGAATATGTGTCGGTCAGACGTCCAGCTGGTATCACTGACCTATACAACACAAAATGTC
7441 tattatgtagtctgttttttatgcaaaatctaatttaatatattgatatttatatcattt 7500
ATAATACATCAGACAAAAAATACGTTTTAGATTAAATTATATAACTATAAATATAGTAAA
XhoI
|
7501 tacgtttctcgttcagctttcttgtacaaagtggttgatatctgactcgagcaccaccac 7560
ATGCAAAGAGCAAGTCGAAAGAACATGTTTCACCAACTATAGACTGAGCTCGTGGTGGTG
T7 term(7580,7708)>>>
|
7561 caccaccactgagatccggctgctaacaaagcccgaaaggaagctgagttggctgctgcc 7620
GTGGTGGTGACTCTAGGCCGACGATTGTTTCGGGCTTTCCTTCGACTCAACCGACGACGG
7621 accgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggtttt 7680
TGGCGACTCGTTATTGATCGTATTGGGGAACCCCGGAGATTTGCCCAGAACTCCCCAAAA
7681 ttgctgaaaggaggaactatatccggat 7708
AACGACTTTCCTCCTTGATATAGGCCTA