piLenti-siRNA
| Vector Name | piLenti-siRNA |
| VectorType | Lentiviral siRNA Vector |
| Antibiotic Information | Bacterial: Kanamycin Mammalian: Puromycin |
| Sequencing Primers | 5’--TACGTCCAAGGTCGGGCAGGAAGA--3’ in the U6 promoter region |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat actctcatga gcggatacat atttgaatgt atttagaaaa
361 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtaactataa
421 cggtcctaag gtagcgaaaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt
481 aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt
541 ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat
601 tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata
661 cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
721 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
781 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
841 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
901 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
961 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
1021 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
1081 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
1141 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
1201 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
1261 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
1321 gaagagcaaa acaaaagtaa gaccaccgca cagcaagccc gctgatcttc agacctggag
1381 gaggagatat gagggacatt ggagaagtga attatataaa tataaagtag taaaaattga
1441 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
1501 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
1561 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
1621 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
1681 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
1741 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
1801 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
1861 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
1921 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
1981 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
2041 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
2101 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg
2161 aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc
2221 tcgacggtat cgaaagcttg ggattcgaat ttaaaagaaa aggggggatt ggggggtaca
2281 gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaactacaaa
2341 aacaaattac aaaaattcaa aattttcggg tttttcgaac ctaggtacgt ccaaggtcgg
2401 gcaggaagag ggcctatttc ccatgattcc ttcatatttg catatacgat acaaggctgt
2461 tagagagata attagaatta atttgactgt aaacacaaag atattagtac aaaatacgtg
2521 acgtagaaag taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga
2581 ctatcatatg cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg
2641 gaaaggacaa aaatagtctt ctctagagat atcatttaaa tgtcgacgaa ttcctcgaga
2701 gaagacagct ttttttggtg tttcgtggtc tcatacagaa cttataagat tcccaaatcc
2761 aaagacattt cacgtttatg gtgatttccc agaacacata gcgacatgca aatattgcag
2821 ggcgccactc ccctgtccct cacagccatc ttcctgccag ggcgcacgcg cgctgggtgt
2881 tcccgcctag tgacactggg cccgcgattc cttggagcgg gttgatgacg tcagcgttct
2941 caattgtagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
3001 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
3061 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
3121 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
3181 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
3241 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
3301 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
3361 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
3421 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
3481 tgggaggtct atataagcag agctggttta gtgaaccgtc agggtaccgg atccatggcc
3541 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtcccccg ggccgtacgc
3601 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga cccggaccgc
3661 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
3721 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
3781 gtcgaagcgg gggcggtgtt cgccgagatc ggctcgcgca tggccgagtt gagcggttcc
3841 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc
3901 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc
3961 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgctg gggtgcccgc cttcctggag
4021 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
4081 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaacg
4141 cgttccggaa atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
4201 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
4261 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
4321 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
4381 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
4441 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
4501 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca
4561 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
4621 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
4681 ccgcgtctcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg
4741 tccggatgga agggctaatt cactcccaac gaatacaaga tctgcttttt gcttgtactg
4801 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
4861 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
4921 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
4981 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
5041 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
5101 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
5161 tcaatgtatc ttatcatgtc tggcatctat gtcgggtgcg gagaaagagg taatgaaatg
5221 gcattatggg tattatgggt ctgcattaat gaatcggcca acggatgtcg gtgtgaaata
5281 ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact
5341 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
5401 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
5461 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
5521 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
5581 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
5641 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
5701 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
5761 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
5821 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
5881 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
5941 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
6001 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
6061 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
6121 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg ccatgagatt atcaaaaagg
6181 atcttcacct agatcctttt cacgtagaaa gccagtccgc agaaacggtg ctgaccccgg
6241 atgaatgtca gctactgggc tatctggaca agggaaaacg caagcgcaaa gagaaagcag
6301 gtagcttgca gtgggcttac atggcgatag ctagactggg cggttttatg gacagcaagc
6361 gaaccggaat tgccagctgg ggcgccctct ggtaaggttg ggaagccctg caaagtaaac
6421 tggatggctt tcttgccgcc aaggatctga tggcgcaggg gatcaagctc tgatcaagag
6481 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc
6541 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
6601 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
6661 tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg
6721 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
6781 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
6841 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
6901 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
6961 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
7021 ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
7081 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
7141 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
7201 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
7261 atcgccttct atcgccttct tgacgagttc ttctgaattt tgttaaaatt tttgttaaat
7321 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
7381 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
7441 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
7501 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
7561 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
7621 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
7681 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtccattc gccattcagg
7741 atcgaattaa ttcttaatta acatcatcaa taatatacct t
LOCUS dna 8221 bp
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
misc_binding 457..462
/dbxref="REBASE:HpaI"
Other Gene 715..895
/gene="HIV-1_5_LTR other"
Other Gene 1006..1050
/gene="HIV-1_psi_pack other"
misc_binding 1092..1097
/dbxref="REBASE:NruI"
Regulatory_Seq 1557..1790
/gene="RRE reg"
ORF 1600..2301
/sequence="ORF_1 rf(1)"
Promoter 2440..2695
/gene="hU6 prom"
misc_binding 2709..2714
/dbxref="REBASE:XbaI"
misc_binding 2715..2720
/dbxref="REBASE:EcoRV"
misc_binding 2735..2740
/dbxref="REBASE:EcoRI"
misc_binding 2741..2746
/dbxref="REBASE:XhoI"
misc_binding 2945..2950
/dbxref="REBASE:ApaI"
ORF 3507..4184
/sequence="ORF_3 rf(3)"
misc_binding 3570..3575
/dbxref="REBASE:KpnI"
misc_binding 3576..3581
/dbxref="REBASE:BamHI"
Marker 3588..4184
/gene="puro marker"
misc_binding 3634..3639
/dbxref="REBASE:XmaI"
misc_binding 3634..3639
/dbxref="REBASE:SmaI"
misc_binding 3914..3919
/dbxref="REBASE:StuI"
Other Gene 4794..4846
/gene="delta_U3 other"
Other Gene 4847..5027
/gene="HIV-1_5_LTR other"
Rep_Origin 5533..6152
/gene="pBR322 origin"
Promoter 6805..6854
/gene="NEOKAN prom"
misc_binding 6912..6917
/dbxref="REBASE:BclI"
ORF 6943..7737
/sequence="ORF_2 rf(1)"
Marker 6946..7734
/gene="NTP_II marker"
Rep_Origin 7842..8147
/gene="f1 origin"
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 ttttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgt 60
AAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCA
61 gggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaa 120
CCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTT
Encap other(162,310)>>>
|
121 cacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgccggtgtacacagg 180
GTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCC
181 aagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagt 240
TTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCA
241 aagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttg 300
TTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAAC
301 tgttactcatagcgcgtaatacggcagacctcagcgctagattattgaagcatttatcag 360
ACAATGAGTATCGCGCATTATGCCGTCTGGAGTCGCGATCTAATAACTTCGTAAATAGTC
amp prom(365,393)<<<
|
361 ggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggg 420
CCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCC
HpaI
|
421 gttccgcgcacatttccccgaaaagtgccacctgacgttaactataacggtcctaaggta 480
CAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAATTGATATTGCCAGGATTCCAT
481 gcgaaaatgtagtcttatgcaatactcttgtagtcttgcaacatggtaacgatgagttag 540
CGCTTTTACATCAGAATACGTTATGAGAACATCAGAACGTTGTACCATTGCTACTCAATC
541 caacatgccttacaaggagagaaaaagcaccgtgcatgccgattggtggaagtaaggtgg 600
GTTGTACGGAATGTTCCTCTCTTTTTCGTGGCACGTACGGCTAACCACCTTCATTCCACC
601 tacgatcgtgccttattaggaaggcaacagacgggtctgacatggattggacgaaccact 660
ATGCTAGCACGGAATAATCCTTCCGTTGTCTGCCCAGACTGTACCTAACCTGCTTGGTGA
HIV-1_5_LTR other(715,895)>>>
|
661 gaattgccgcattgcagagatattgtatttaagtgcctagctcgatacataaacgggtct 720
CTTAACGGCGTAACGTCTCTATAACATAAATTCACGGATCGAGCTATGTATTTGCCCAGA
721 ctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgctt 780
GAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAA
781 aagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgac 840
TTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTG
841 tctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggc 900
AGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCACCG
901 gcccgaacagggacttgaaagcgaaagggaaaccagaggagctctctcgacgcaggactc 960
CGGGCTTGTCCCTGAACTTTCGCTTTCCCTTTGGTCTCCTCGAGAGAGCTGCGTCCTGAG
HIV-1_psi_pack other(1006,1050)>>>
|
961 ggcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaa 1020
CCGAACGACTTCGCGCGTGCCGTTCTCCGCTCCCCGCCGCTGACCACTCATGCGGTTTTT
1021 ttttgactagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcggg 1080
AAAACTGATCGCCTCCGATCTTCCTCTCTCTACCCACGCTCTCGCAGTCATAATTCGCCC
NruI
|
1081 ggagaattagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatata 1140
CCTCTTAATCTAGCGCTACCCTTTTTTAAGCCAATTCCGGTCCCCCTTTCTTTTTTATAT
1141 aattaaaacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcc 1200
TTAATTTTGTATATCATACCCGTTCGTCCCTCGATCTTGCTAAGCGTCAATTAGGACCGG
1201 tgttagaaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcaga 1260
ACAATCTTTGTAGTCTTCCGACATCTGTTTATGACCCTGTCGATGTTGGTAGGGAAGTCT
1261 caggatcagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatc 1320
GTCCTAGTCTTCTTGAATCTAGTAATATATTATGTCATCGTTGGGAGATAACACACGTAG
1321 aaaggatagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaaca 1380
TTTCCTATCTCTATTTTCTGTGGTTCCTTCGAAATCTGTTCTATCTCCTTCTCGTTTTGT
1381 aaagtaagaccaccgcacagcaagcccgctgatcttcagacctggaggaggagatatgag 1440
TTTCATTCTGGTGGCGTGTCGTTCGGGCGACTAGAAGTCTGGACCTCCTCCTCTATACTC
1441 ggacattggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagta 1500
CCTGTAACCTCTTCACTTAATATATTTATATTTCATCATTTTTAACTTGGTAATCCTCAT
RRE reg(1557,1790)>>>
|
1501 gcacccaccaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaatagga 1560
CGTGGGTGGTTCCGTTTCTCTTCTCACCACGTCTCTCTTTTTTCTCGTCACCCTTATCCT
ORF_1 rf(1)(1600,2301)>>>
|
1561 gctttgttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacg 1620
CGAAACAAGGAACCCAAGAACCCTCGTCGTCCTTCGTGATACCCGCGTCGCAGTTACTGC
1621 ctgacggtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctg 1680
GACTGCCATGTCCGGTCTGTTAATAACAGACCATATCACGTCGTCGTCTTGTTAAACGAC
1681 agggctattgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctc 1740
TCCCGATAACTCCGCGTTGTCGTAGACAACGTTGAGTGTCAGACCCCGTAGTTCGTCGAG
1741 caggcaagaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttgg 1800
GTCCGTTCTTAGGACCGACACCTTTCTATGGATTTCCTAGTTGTCGAGGACCCCTAAACC
1801 ggttgctctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaat 1860
CCAACGAGACCTTTTGAGTAAACGTGGTGACGACACGGAACCTTACGATCAACCTCATTA
1861 aaatctctggaacagatttggaatcacacgacctggatggagtgggacagagaaattaac 1920
TTTAGAGACCTTGTCTAAACCTTAGTGTGCTGGACCTACCTCACCCTGTCTCTTTAATTG
1921 aattacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaat 1980
TTAATGTGTTCGAATTATGTGAGGAATTAACTTCTTAGCGTTTTGGTCGTTCTTTTCTTA
1981 gaacaagaattattggaattagataaatgggcaagtttgtggaattggtttaacataaca 2040
CTTGTTCTTAATAACCTTAATCTATTTACCCGTTCAAACACCTTAACCAAATTGTATTGT
2041 aattggctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaaga 2100
TTAACCGACACCATATATTTTAATAAGTATTACTATCATCCTCCGAACCATCCAAATTCT
2101 atagtttttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcg 2160
TATCAAAAACGACATGAAAGATATCACTTATCTCAATCCGTCCCTATAAGTGGTAATAGC
2161 tttcagacccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaa 2220
AAAGTCTGGGTGGAGGGTTGGGGCTCCCCTGGGCTGTCCGGGCTTCCTTATCTTCTTCTT
2221 ggtggagagagagacagagacagatccattcgattagtgaacggatctcgacggtatcga 2280
CCACCTCTCTCTCTGTCTCTGTCTAGGTAAGCTAATCACTTGCCTAGAGCTGCCATAGCT
2281 aagcttgggattcgaatttaaaagaaaaggggggattggggggtacagtgcaggggaaag 2340
TTCGAACCCTAAGCTTAAATTTTCTTTTCCCCCCTAACCCCCCATGTCACGTCCCCTTTC
2341 aatagtagacataatagcaacagacatacaaactaaagaactacaaaaacaaattacaaa 2400
TTATCATCTGTATTATCGTTGTCTGTATGTTTGATTTCTTGATGTTTTTGTTTAATGTTT
hU6 prom(2440,2695)>>>
|
2401 aattcaaaattttcgggtttttcgaacctaggtacgtccaaggtcgggcaggaagagggc 2460
TTAAGTTTTAAAAGCCCAAAAAGCTTGGATCCATGCAGGTTCCAGCCCGTCCTTCTCCCG
2461 ctatttcccatgattccttcatatttgcatatacgatacaaggctgttagagagataatt 2520
GATAAAGGGTACTAAGGAAGTATAAACGTATATGCTATGTTCCGACAATCTCTCTATTAA
2521 agaattaatttgactgtaaacacaaagatattagtacaaaatacgtgacgtagaaagtaa 2580
TCTTAATTAAACTGACATTTGTGTTTCTATAATCATGTTTTATGCACTGCATCTTTCATT
2581 taatttcttgggtagtttgcagttttaaaattatgttttaaaatggactatcatatgctt 2640
ATTAAAGAACCCATCAAACGTCAAAATTTTAATACAAAATTTTACCTGATAGTATACGAA
2641 accgtaacttgaaagtatttcgatttcttggctttatatatcttgtggaaaggacaaaaa 2700
TGGCATTGAACTTTCATAAAGCTAAAGAACCGAAATATATAGAACACCTTTCCTGTTTTT
XbaI EcoRV EcoRI XhoI
| | | |
2701 tagtcttctctagagatatcatttaaatgtcgacgaattcctcgagagaagacagctttt 2760
ATCAGAAGAGATCTCTATAGTAAATTTACAGCTGCTTAAGGAGCTCTCTTCTGTCGAAAA
2761 tttggtgtttcgtggtctcatacagaacttataagattcccaaatccaaagacatttcac 2820
AAACCACAAAGCACCAGAGTATGTCTTGAATATTCTAAGGGTTTAGGTTTCTGTAAAGTG
2821 gtttatggtgatttcccagaacacatagcgacatgcaaatattgcagggcgccactcccc 2880
CAAATACCACTAAAGGGTCTTGTGTATCGCTGTACGTTTATAACGTCCCGCGGTGAGGGG
2881 tgtccctcacagccatcttcctgccagggcgcacgcgcgctgggtgttcccgcctagtga 2940
ACAGGGAGTGTCGGTAGAAGGACGGTCCCGCGTGCGCGCGACCCACAAGGGCGGATCACT
ApaI
|
2941 cactgggcccgcgattccttggagcgggttgatgacgtcagcgttctcaattgtagtaat 3000
GTGACCCGGGCGCTAAGGAACCTCGCCCAACTACTGCAGTCGCAAGAGTTAACATCATTA
3001 caattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacgg 3060
GTTAATGCCCCAGTAATCAAGTATCGGGTATATACCTCAAGGCGCAATGTATTGAATGCC
3061 taaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgt 3120
ATTTACCGGGCGGACCGACTGGCGGGTTGCTGGGGGCGGGTAACTGCAGTTATTACTGCA
3121 atgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttac 3180
TACAAGGGTATCATTGCGGTTATCCCTGAAAGGTAACTGCAGTTACCCACCTCATAAATG
3181 ggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattg 3240
CCATTTGACGGGTGAACCGTCATGTAGTTCACATAGTATACGGTTCATGCGGGGGATAAC
3241 acgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggact 3300
TGCAGTTACTGCCATTTACCGGGCGGACCGTAATACGGGTCATGTACTGGAATACCCTGA
3301 ttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggtttt 3360
AAGGATGAACCGTCATGTAGATGCATAATCAGTAGCGATAATGGTACCACTACGCCAAAA
3361 ggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccacc 3420
CCGTCATGTAGTTACCCGCACCTATCGCCAAACTGAGTGCCCCTAAAGGTTCAGAGGTGG
3421 ccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtc 3480
GGTAACTGCAGTTACCCTCAAACAAAACCGTGGTTTTAGTTGCCCTGAAAGGTTTTACAG
ORF_3 rf(3)(3507,4184)>>>
|
3481 gtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctata 3540
CATTGTTGAGGCGGGGTAACTGCGTTTACCCGCCATCCGCACATGCCACCCTCCAGATAT
BamHI
|
KpnI puro marker(3588,4184)>>>
| | |
3541 taagcagagctggtttagtgaaccgtcagggtaccggatccatggccaccgagtacaagc 3600
ATTCGTCTCGACCAAATCACTTGGCAGTCCCATGGCCTAGGTACCGGTGGCTCATGTTCG
SmaI
|
XmaI
| |
3601 ccacggtgcgcctcgccacccgcgacgacgtcccccgggccgtacgcaccctcgccgccg 3660
GGTGCCACGCGGAGCGGTGGGCGCTGCTGCAGGGGGCCCGGCATGCGTGGGAGCGGCGGC
3661 cgttcgccgactaccccgccacgcgccacaccgtcgacccggaccgccacatcgagcggg 3720
GCAAGCGGCTGATGGGGCGGTGCGCGGTGTGGCAGCTGGGCCTGGCGGTGTAGCTCGCCC
3721 tcaccgagctgcaagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtggg 3780
AGTGGCTCGACGTTCTTGAGAAGGAGTGCGCGCAGCCCGAGCTGTAGCCGTTCCACACCC
3781 tcgcggacgacggcgccgcggtggcggtctggaccacgccggagagcgtcgaagcggggg 3840
AGCGCCTGCTGCCGCGGCGCCACCGCCAGACCTGGTGCGGCCTCTCGCAGCTTCGCCCCC
3841 cggtgttcgccgagatcggctcgcgcatggccgagttgagcggttcccggctggccgcgc 3900
GCCACAAGCGGCTCTAGCCGAGCGCGTACCGGCTCAACTCGCCAAGGGCCGACCGGCGCG
StuI
|
3901 agcaacagatggaaggcctcctggcgccgcaccggcccaaggagcccgcgtggttcctgg 3960
TCGTTGTCTACCTTCCGGAGGACCGCGGCGTGGCCGGGTTCCTCGGGCGCACCAAGGACC
3961 ccaccgtcggcgtctcgcccgaccaccagggcaagggtctgggcagcgccgtcgtgctcc 4020
GGTGGCAGCCGCAGAGCGGGCTGGTGGTCCCGTTCCCAGACCCGTCGCGGCAGCACGAGG
4021 ccggagtggaggcggccgagcgcgctggggtgcccgccttcctggagacctccgcgcccc 4080
GGCCTCACCTCCGCCGGCTCGCGCGACCCCACGGGCGGAAGGACCTCTGGAGGCGCGGGG
4081 gcaacctccccttctacgagcggctcggcttcaccgtcaccgccgacgtcgaggtgcccg 4140
CGTTGGAGGGGAAGATGCTCGCCGAGCCGAAGTGGCAGTGGCGGCTGCAGCTCCACGGGC
4141 aaggaccgcgcacctggtgcatgacccgcaagcccggtgcctgaacgcgttccggaaatc 4200
TTCCTGGCGCGTGGACCACGTACTGGGCGTTCGGGCCACGGACTTGCGCAAGGCCTTTAG
4201 aacctctggattacaaaatttgtgaaagattgactggtattcttaactatgttgctcctt 4260
TTGGAGACCTAATGTTTTAAACACTTTCTAACTGACCATAAGAATTGATACAACGAGGAA
4261 ttacgctatgtggatacgctgctttaatgcctttgtatcatgctattgcttcccgtatgg 4320
AATGCGATACACCTATGCGACGAAATTACGGAAACATAGTACGATAACGAAGGGCATACC
4321 ctttcattttctcctccttgtataaatcctggttgctgtctctttatgaggagttgtggc 4380
GAAAGTAAAAGAGGAGGAACATATTTAGGACCAACGACAGAGAAATACTCCTCAACACCG
4381 ccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccccactggtt 4440
GGCAACAGTCCGTTGCACCGCACCACACGTGACACAAACGACTGCGTTGGGGGTGACCAA
4441 ggggcattgccaccacctgtcagctcctttccgggactttcgctttccccctccctattg 4500
CCCCGTAACGGTGGTGGACAGTCGAGGAAAGGCCCTGAAAGCGAAAGGGGGAGGGATAAC
4501 ccacggcggaactcatcgccgcctgccttgcccgctgctggacaggggctcggctgttgg 4560
GGTGCCGCCTTGAGTAGCGGCGGACGGAACGGGCGACGACCTGTCCCCGAGCCGACAACC
4561 gcactgacaattccgtggtgttgtcggggaagctgacgtcctttccatggctgctcgcct 4620
CGTGACTGTTAAGGCACCACAACAGCCCCTTCGACTGCAGGAAAGGTACCGACGAGCGGA
4621 gtgttgccacctggattctgcgcgggacgtccttctgctacgtcccttcggccctcaatc 4680
CACAACGGTGGACCTAAGACGCGCCCTGCAGGAAGACGATGCAGGGAAGCCGGGAGTTAG
4681 cagcggaccttccttcccgcggcctgctgccggctctgcggcctcttccgcgtctcgcct 4740
GTCGCCTGGAAGGAAGGGCGCCGGACGACGGCCGAGACGCCGGAGAAGGCGCAGAGCGGA
delta_U3 other(4794,4846)>>>
|
4741 tcgccctcagacgagtcggatctccctttgggccgcctccccgcctgtccggatggaagg 4800
AGCGGGAGTCTGCTCAGCCTAGAGGGAAACCCGGCGGAGGGGCGGACAGGCCTACCTTCC
HIV-1_5_LTR other(4847,5027)>>>
|
4801 gctaattcactcccaacgaatacaagatctgctttttgcttgtactgggtctctctggtt 4860
CGATTAAGTGAGGGTTGCTTATGTTCTAGACGAAAAACGAACATGACCCAGAGAGACCAA
4861 agaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcctca 4920
TCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAATTCGGAGT
4921 ataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaa 4980
TATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTGAGACCATT
4981 ctagagatccctcagacccttttagtcagtgtggaaaatctctagcagtagtagttcatg 5040
GATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCATCATCAAGTAC
5041 tcatcttattattcagtatttataacttgcaaagaaatgaatatcagagagtgagaggaa 5100
AGTAGAATAATAAGTCATAAATATTGAACGTTTCTTTACTTATAGTCTCTCACTCTCCTT
5101 cttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaa 5160
GAACAAATAACGTCGAATATTACCAATGTTTATTTCGTTATCGTAGTGTTTAAAGTGTTT
5161 taaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatctta 5220
ATTTCGTAAAAAAAGTGACGTAAGATCAACACCAAACAGGTTTGAGTAGTTACATAGAAT
5221 tcatgtctggcatctatgtcgggtgcggagaaagaggtaatgaaatggcattatgggtat 5280
AGTACAGACCGTAGATACAGCCCACGCCTCTTTCTCCATTACTTTACCGTAATACCCATA
5281 tatgggtctgcattaatgaatcggccaacgatcccggtgtgaaataccgcacagatgcgt 5340
ATACCCAGACGTAATTACTTAGCCGGTTGCTAGGGCCACACTTTATGGCGTGTCTACGCA
5341 aaggagaaaataccgcatcaggcgctcttccgcttcctcgctcactgactcgctgcgctc 5400
TTCCTCTTTTATGGCGTAGTCCGCGAGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGAG
5401 ggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccac 5460
CCAGCAAGCCGACGCCGCTCGCCATAGTCGAGTGAGTTTCCGCCATTATGCCAATAGGTG
5461 agaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaa 5520
TCTTAGTCCCCTATTGCGTCCTTTCTTGTACACTCGTTTTCCGGTCGTTTTCCGGTCCTT
pBR322 origin(5533,6152)<<<
|
5521 ccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatca 5580
GGCATTTTTCCGGCGCAACGACCGCAAAAAGGTATCCGAGGCGGGGGGACTGCTCGTAGT
5581 caaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggc 5640
GTTTTTAGCTGCGAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGATATTTCTATGGTCCG
5641 gtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggata 5700
CAAAGGGGGACCTTCGAGGGAGCACGCGAGAGGACAAGGCTGGGACGGCGAATGGCCTAT
5701 cctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggta 5760
GGACAGGCGGAAAGAGGGAAGCCCTTCGCACCGCGAAAGAGTATCGAGTGCGACATCCAT
5761 tctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttca 5820
AGAGTCAAGCCACATCCAGCAAGCGAGGTTCGACCCGACACACGTGCTTGGGGGGCAAGT
5821 gcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacga 5880
CGGGCTGGCGACGCGGAATAGGCCATTGATAGCAGAACTCAGGTTGGGCCATTCTGTGCT
5881 cttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcgg 5940
GAATAGCGGTGACCGTCGTCGGTGACCATTGTCCTAATCGTCTCGCTCCATACATCCGCC
5941 tgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttgg 6000
ACGATGTCTCAAGAACTTCACCACCGGATTGATGCCGATGTGATCTTCCTGTCATAAACC
6001 tatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccgg 6060
ATAGACGCGAGACGACTTCGGTCAATGGAAGCCTTTTTCTCAACCATCGAGAACTAGGCC
6061 caaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcag 6120
GTTTGTTTGGTGGCGACCATCGCCACCAAAAAAACAAACGTTCGTCGTCTAATGCGCGTC
6121 aaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaa 6180
TTTTTTTCCTAGAGTTCTTCTAGGAAACTAGAAAAGATGCCCCAGACTGCGAGTCACCTT
6181 cgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagat 6240
GCTTTTGAGTGCAATTCCCTAAAACCAGTACTCTAATAGTTTTTCCTAGAAGTGGATCTA
6241 ccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtc 6300
GGAAAATTTAATTTTTACTTCAAAATTTAGTTAGATTTCATATATACTCATTTGAACCAG
6301 tgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttc 6360
ACTGTCAATGGTTACGAATTAGTCACTCCGTGGATAGAGTCGCTAGACAGATAAAGCAAG
6361 atccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatc 6420
TAGGTATCAACGGACTGAGGGGCAGCACATCTATTGATGCTATGCCCTCCCGAATGGTAG
6421 tggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagc 6480
ACCGGGGTCACGACGTTACTATGGCGCTCTGGGTGCGAGTGGCCGAGGTCTAAATAGTCG
6481 aataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctc 6540
TTATTTGGTCGGTCGGCCTTCCCGGCTCGCGTCTTCACCAGGACGTTGAAATAGGCGGAG
6541 catccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagttt 6600
GTAGGTCAGATAATTAACAACGGCCCTTCGATCTCATTCATCAAGCGGTCAATTATCAAA
6601 gcgcaacgttgttgaaaaaggatcttcacctagatccttttcacgtagaaagccagtccg 6660
CGCGTTGCAACAACTTTTTCCTAGAAGTGGATCTAGGAAAAGTGCATCTTTCGGTCAGGC
6661 cagaaacggtgctgaccccggatgaatgtcagctactgggctatctggacaagggaaaac 6720
GTCTTTGCCACGACTGGGGCCTACTTACAGTCGATGACCCGATAGACCTGTTCCCTTTTG
6721 gcaagcgcaaagagaaagcaggtagcttgcagtgggcttacatggcgatagctagactgg 6780
CGTTCGCGTTTCTCTTTCGTCCATCGAACGTCACCCGAATGTACCGCTATCGATCTGACC
NEOKAN prom(6805,6854)>>>
|
6781 gcggttttatggacagcaagcgaaccggaattgccagctggggcgccctctggtaaggtt 6840
CGCCAAAATACCTGTCGTTCGCTTGGCCTTAACGGTCGACCCCGCGGGAGACCATTCCAA
6841 gggaagccctgcaaagtaaactggatggctttctcgccgccaaggatctgatggcgcagg 6900
CCCTTCGGGACGTTTCATTTGACCTACCGAAAGAGCGGCGGTTCCTAGACTACCGCGTCC
NTP_II marker(6946,7734)>>>
|
BclI ORF_2 rf(1)(6943,7737)>>>
| | |
6901 ggatcaagctctgatcaagagacaggatgaggatcgtttcgcatgattgaacaagatgga 6960
CCTAGTTCGAGACTAGTTCTCTGTCCTACTCCTAGCAAAGCGTACTAACTTGTTCTACCT
6961 ttgcacgcaggttctccggccgcttgggtggagaggctattcggctatgactgggcacaa 7020
AACGTGCGTCCAAGAGGCCGGCGAACCCACCTCTCCGATAAGCCGATACTGACCCGTGTT
7021 cagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggggcgcccggtt 7080
GTCTGTTAGCCGACGAGACTACGGCGGCACAAGGCCGACAGTCGCGTCCCCGCGGGCCAA
7081 ctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaagacgaggcagcgcgg 7140
GAAAAACAGTTCTGGCTGGACAGGCCACGGGACTTACTTGACGTTCTGCTCCGTCGCGCC
7141 ctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactgaa 7200
GATAGCACCGACCGGTGCTGCCCGCAAGGAACGCGTCGACACGAGCTGCAACAGTGACTT
7201 gcgggaagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcac 7260
CGCCCTTCCCTGACCGACGATAACCCGCTTCACGGCCCCGTCCTAGAGGACAGTAGAGTG
7261 cttgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgctt 7320
GAACGAGGACGGCTCTTTCATAGGTAGTACCGACTACGTTACGCCGCCGACGTATGCGAA
7321 gatccggctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtact 7380
CTAGGCCGATGGACGGGTAAGCTGGTGGTTCGCTTTGTAGCGTAGCTCGCTCGTGCATGA
7381 cggatggaagccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcg 7440
GCCTACCTTCGGCCAGAACAGCTAGTCCTACTAGACCTGCTTCTCGTAGTCCCCGAGCGC
7441 ccagccgaactgttcgccaggctcaaggcgagcatgcccgacggcgaggatctcgtcgtg 7500
GGTCGGCTTGACAAGCGGTCCGAGTTCCGCTCGTACGGGCTGCCGCTCCTAGAGCAGCAC
7501 acccatggcgatgcctgcttgccgaatatcatggtggaaaatggccgcttttctggattc 7560
TGGGTACCGCTACGGACGAACGGCTTATAGTACCACCTTTTACCGGCGAAAAGACCTAAG
7561 atcgactgtggccggctgggtgtggcggaccgctatcaggacatagcgttggctacccgt 7620
TAGCTGACACCGGCCGACCCACACCGCCTGGCGATAGTCCTGTATCGCAACCGATGGGCA
7621 gatattgctgaagagcttggcggcgaatgggctgaccgcttcctcgtgctttacggtatc 7680
CTATAACGACTTCTCGAACCGCCGCTTACCCGACTGGCGAAGGAGCACGAAATGCCATAG
7681 gccgctcccgattcgcagcgcatcgccttctatcgccttcttgacgagttcttctgaatt 7740
CGGCGAGGGCTAAGCGTCGCGTAGCGGAAGATAGCGGAAGAACTGCTCAAGAAGACTTAA
7741 ttgttaaaatttttgttaaatcagctcattttttaaccaataggccgaaatcggcaacat 7800
AACAATTTTAAAAACAATTTAGTCGAGTAAAAAATTGGTTATCCGGCTTTAGCCGTTGTA
f1 origin(7842,8147)<<<
|
7801 cccttataaatcaaaagaatagaccgcgatagggttgagtgttgttccagtttggaacaa 7860
GGGAATATTTAGTTTTCTTATCTGGCGCTATCCCAACTCACAACAAGGTCAAACCTTGTT
7861 gagtccactattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcaggg 7920
CTCAGGTGATAATTTCTTGCACCTGAGGTTGCAGTTTCCCGCTTTTTGGCAGATAGTCCC
7921 cgatggcccactacgtgaaccatcacccaaatcaagttttttgcggtcgaggtgccgtaa 7980
GCTACCGGGTGATGCACTTGGTAGTGGGTTTAGTTCAAAAAACGCCAGCTCCACGGCATT
7981 agctctaaatcggaaccctaaagggagcccccgatttagagcttgacggggaaagccggc 8040
TCGAGATTTAGCCTTGGGATTTCCCTCGGGGGCTAAATCTCGAACTGCCCCTTTCGGCCG
8041 gaacgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaag 8100
CTTGCACCGCTCTTTCCTTCCCTTCTTTCGCTTTCCTCGCCCGCGATCCCGCGACCGTTC
8101 tgtagcggtcacgctgcgcgtaaccaccacacccgcgcgcttaatgcgccgctacagggc 8160
ACATCGCCAGTGCGACGCGCATTGGTGGTGTGGGCGCGCGAATTACGCGGCGATGTCCCG
8161 gcgtccattcgccattcaggatcgaattaattcttaattaacatcatcaataatatacct 8220
CGCAGGTAAGCGGTAAGTCCTAGCTTAATTAAGAATTAATTGTAGTAGTTATTATATGGA
8221 t 8221
A