OneScript® Hot 5X RT MasterMix
Cat. No.
G590
Unit
100 rxn (400 µl)
Price
$209.00
$189.00
| Cat. No. | G590 | ||||||||||
| Name | OneScript® Hot 5X RT MasterMix | ||||||||||
| Unit | 100 rxn (400 µl) | ||||||||||
| Category | Reverse Transcriptase & RT-PCR | ||||||||||
| Description |
OneScript® Hot 5X RT MasterMix is a convenient, ready-to-use formulation designed for efficient first-strand cDNA synthesis. This product simplifies the cDNA synthesis process, ensuring reliability and reproducibility. The optimized 5X RT MasterMix contains abm’s proprietary OneScript® Hot Reverse Transcriptase, RNaseOFF Ribonuclease Inhibitor, dNTPs, and a carefully balanced ratio of Oligo(dT)s and Random Primers. The resulting high-quality cDNA is suitable for a wide range of downstream applications. Product Features:
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| Application |
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| Concentration | 5X | ||||||||||
| Storage Condition |
Store at -20°C. |
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| Note |
If input RNA samples are expected to have high levels of genomic DNA contamination, we recommend using abm’s All-In-One 5X RT MasterMix with gDNA Removal (Cat. No. G592). |
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| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G590 |
Datasheet
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Can I use both total RNA and poly(A)+ mRNA for cDNA synthesis?
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Yes, both total RNA and poly(A)+ mRNA can be used, though poly(A)+ mRNA typically yields higher quantities and better purity.
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Can I synthesize cDNA from long RNA transcripts?
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Yes, for longer RNA transcripts, extend the 60°C incubation time to up to 30 minutes.
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| What can I use the synthesized cDNA for? | |
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The high-quality cDNA can be used in a variety of downstream applications, including gene expression analysis, cloning, and PCR-based assays. BlasTaq™ 2X qPCR Master Mix (Cat. No. G891) is well suited to downstream qPCR applications.
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| How should I store the synthesized cDNA? | |
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Store the synthesized first-strand cDNA at -20°C for long-term use.
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| Why am I getting low cDNA yields? | |
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Low cDNA yields can be caused by poor RNA integrity, contamination, or insufficient RNA input. To improve yield:
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| How much RNA template should I use for cDNA synthesis? | |
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We recommend using 1 ng to 2 μg of RNA per reaction.
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What volumes of cDNA should I use for downstream PCR?
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Typically, use 1 μl of cDNA in a 25 μl PCR reaction. You can add up to 20% of the PCR volume (e.g., 5 μl in a 25 μl PCR), depending on your target and primers.
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