Recombinant Human EPO (CHO cells)
Cat. No.
Z101435
Unit
Price
$81.00
| Cat. No. | Z101435 |
| Name | Recombinant Human EPO (CHO cells) |
| Description | EPO is predominantly synthesized and secreted by tubular and juxtatubular capillary, endothelial, and interstitial cells of the kidney. Approximately 10-15% of the total amount of EPO comes from extrarenal sources and is predominantly produced by hepatocytes and Kupffer cells of the liver. Approximately 40% of the molecular mass of EPO is due to its glycosylation. Glycosylation is an important factor determining the pharmacokinetic behaviour of EPO in vivo. Non-glycosylated Epo has an extremely short biological half life. Recombinant Human EPO is a glycosylated protein that runs at approximately 35 kDa owing to its glycosylation. |
| Organism | Human (H. sapiens) |
| Unit quantity | 50 µg |
| Family | HDGF/S-100/EPO(TPO) Families |
| Aliases | DBAL, ECYT5, EP, MVCD2 |
| Gene Symbol | EPO |
| Gene ID | 2056 |
| Accession Number | P01588 |
| Source | CHO cells |
| Species | Human (H. sapiens) |
| Appearance | Lyophilized Powder |
| Molecular Weight | 20 kDa |
| Endotoxin Level | <1.0 EU/µg of recombinant protein as determined by the LAL method |
| Purity | >95% as determined by SDS-PAGE |
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS (pH 7) |
| Function | Activity was determined by the dose-dependent proliferation assay using a factor-dependent human erythroleukemic cell line TF-1 and was found to be less than 0.2ng/ml |
| Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. |
| Storage | The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
| Usage | For research use only. Not for diagnostic or therapeutic use. |
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z101435 |
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