Mycoplasma Pro PCR Detection & Elimination Bundle
| Cat. No. | G239-G398 |
| Name | Mycoplasma Pro PCR Detection & Elimination Bundle |
| Description |
Enjoy superior performance at lower costs! abm's Mycoplasma Pro PCR Detection & Elimination Bundle combines the power of our stategically improved kit Mycoplasma Pro PCR Detection Kit (Cat. No. G239) and MycoAway™ Mycoplasma Elimination Cocktail (1000X) (Cat. No. G398). The Mycoplasma Pro PCR Detection Kit is an improved version of our best-selling kit (Cat. No. G238) with a modified Pro Primer Mix and more stringent cycling conditions to include the detection of additional Mycoplasma and Acholeplasma strains such as A. laidlawii. An internal PCR control is also included to help confidently distinguish between a genuine negative and a false negative result due to technical errors or PCR inhibition. Furthermore, the MycoAway™ Mycoplasma Elimination Cocktail (1000X) is a specially formulated agent to effectively clear cells from Mycoplasma contamination in both adherent and suspension cells. Our potent formulation is non-toxic and eliminates 70+ species of Mycoplasma in as little as 2-3 weeks (4-6 cell passages)! The combination of these two kits ensures complete protection of your cell lines! |
| Unit | Bundle |
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G239-G398 |
| How do I use the Mycoplasma PCR Detection Kit in conjunction with the MycoAway™ Elimination Cocktail (1000X)? | |
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Mycoplasma (Cat. No. G238) or Mycoplasma Pro (Cat. No. G239) Detection Kits should be used first in order to identify contaminated cells. Either product will only require a small volume (2.5 μl) of culture supernatant. If cells are Mycoplasma-positive, treat cells with MycoAway™ Elimination Cocktail (1000X) (Cat. No. G398).
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| I did not see amplification of the internal PCR Band. What should I do? | |
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PCR amplification can be inhibited by excessive cells, debris, and/or byproducts. If PCR inhibition is noted and the 180 bp internal PCR control band is not seen, dilute the media sample with Nuclease-Free Water until the internal PCR control band is clearly seen (recommended dilution range 1/10 or 1/100).
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| We noticed that the band size for the positive control on agarose gel was around 400 bp, while the datasheet indicated it should be around 500 bp. Should we be concerned about this discrepancy? | |
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No, this is a normal observation. The positive control band should be strong and bright, with an expected size range between 400-500 bp on a well-resolved 2% gel. As long as the band is strong and clearly visible, it falls within the expected range, and there is no issue with the positive control.
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