Nucleus Labeling Lentivirus (LMNB1)(GFP)
Cat. No.
LVP706
Unit
2 x 100µl
Price
$969.00
| Cat. No. | LVP706 |
| Name | Nucleus Labeling Lentivirus (LMNB1)(GFP) |
| Unit | 2 x 100µl |
| Description |
This Nucleus Labeling Lentivirus can be used to easily label the nucleus. The vector expresses GFP (Green Fluorescent Protein) fused to the N-terminus of the lamin B1 protein (LMNB1), which is a component of the nuclear lamina. This lentivirus offers a simple method to visualize the nucleus which doesn’t require antibodies or chemical dyes. Combine with other organelle labeling lentiviruses for multiplexed labeling. |
| Applications |
|
| Vector | pLenti-CMV-eGFP-LMNB1 |
| Titer | 109 IU/mL |
| Storage Condition |
For long term storage, it is recommended to store the viruses at -80°C in small aliquots to avoid repeated freeze-thaw cycles. |
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. LVP706 |
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Supporting Protocol
| How long after transduction can the infection efficiency be observed? | |
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You can observe transduction efficiency from 48 hours up to 5 days after infection.
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| What are the primers to use for SV40T and SV40T tsA58 detection? | |
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PCR primers:
SV40T Forward Primer Sequence
5’ AGCCTGTAGAACCAAACATT 3'
SV40T Reverse Primer Sequence
5’ CTGCTGACTCTCAACATTCT 3'
The two primers should amplify the region between 3677-4468bp, giving a 792bp fragment.
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| What is the sequence of the SV40 large T antigen? | |
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This information can be accessed on this page by clicking on "pLenti-SV40-T" under vector map. The Large T antigen is at position 5079-5927.
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| For G221 and LV620, what does the 'V12' in RasV12 mean? | |
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The V12 means that amino acid # 12 is mutated from a Valine to a Glycine. Other than that, the sequence matches the coding region of HRAS perfectly (NM_005343).
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| Where is the SV40T tsA58 gene sequence? | |
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The SV40T tsA58 gene is located between 3138-5264bp, with the Alanine-to-Valine mutation at amino acid 438.
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- Bayati, A et al. (2022). "Rapid macropinocytic transfer of α-synuclein to lysosomes." Cell Reports. 40(3):111102. DOI: 10.1016/j.celrep.2022.111102. Application: Organelle Labeling Lentivirus.
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