Mycoplasma PCR Detection & Elimination Bundle
|Mycoplasma PCR Detection & Elimination Bundle
Enjoy superior performance at lower costs!
abm's Mycoplasma PCR Detection & Elimination Bundle combines the power of our bestselling kit Mycoplasma PCR Detection Kit (Cat. No. G238) and MycoAway™ Mycoplasma Elimination Cocktail (1000X) (Cat. No. G398).
The Mycoplasma PCR Detection Kit offers highly specific and sensitive detection of 200+ strains of Mycoplasmas in less than 2 hours. This bestselling kit is designed to minimize false positives, ensuring quick and reliable routine screening of cell cultures. A recent official publication by a national public health agency found that our kit out-performs Sigma and Lonza's kits while being 6X less expensive than ATCC's kit (Russell et al., 2020). Furthermore, the MycoAway™ Mycoplasma Elimination Cocktail (1000X) is a specially formulated agent to effectively clear cells from Mycoplasma contamination in both adherent and suspension cells. Our potent formulation is non-toxic and eliminates 70+ species of Mycoplasma in as little as 2-3 weeks (4-6 cell passages)! The combination of these two kits ensures complete protection of your cell lines!
|If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G238-G398
|What is the basis of the positive control in the mycoplasma kit?
It is DNA fragments of Mycoplasma gDNA diluted in medium.
|How much should I use for a T75 flask?
For a T75 flask, we recommend using 5ml - 7ml of our Applied Cell Extracellular Matrix (G422).
|What is the country of origin of TM999 FBS?
|There is a ~200-250bp band in one of my samples, does this mean it is mycoplasma positive?
In rare cases, there may be a weak non-specific binding to a region of human gDNA due to the degenerate nature of the primers that can amplify a weak 250bp band in human cell samples. Your sample can be considered as negative for mycoplasma, as mycoplasma amplicons will be 370-550bp in size. You can try to increase the annealing temperature of the PCR program to 60°C to reduce this non-specific amplification.
|I did not see amplification of the positive control. What can I try?
This could occur in cases where the PCR machine does not ramp temperature fast enough. We suggest trying touchdown PCR in these situations.
|Can collected supernatant be stored at 4 degrees Celcius until we are ready to run the test?
Yes, the supernatant can be stored at 4°C for a few weeks.
|When collecting supernatant, there is a risk of pipetting cell debris. Can cell debris cause false positive bands? Is centrifugation required?
Typically centrifugation is not required, however if your sample looks particularly cloudy, you can centrifuge it before use.
|After running the PCR program, can the PCR reactions be left in the PCR machine overnight at 12 or 4 degrees Celsius?
Yes it can be left in the PCR overnight at 12°C or 4°C.
|Should PCR components be aliquoted to minimize multiple freeze/thaw cycles?
For best results, PCR components should be aliquoted if you are frequently testing small quantites of samples. E.g. If you are only testing 1-5 samples each time, aliquoting is recommended to reduce the number of freeze/thaw cycles.
|Will the serum in my samples inhibit the PCR reaction?
Serum will not have any inhibitory effects on PCR. However, if your media contains >20% serum and if it is too viscous, you can dilute with either basal media or Nuclease-free water by tenfold prior to mycoplasma detection.
|How do I use the Mycoplasma PCR Detection Kit in conjunction with the MycoAway™ Elimination Cocktail (1000X)?
Mycoplasma (Cat. No. G238) or Mycoplasma Pro (Cat. No. G239) Detection Kits should be used first in order to identify contaminated cells. Either product will only require a small volume (2.5 μl) of culture supernatant. If cells are Mycoplasma-positive, treat cells with MycoAway™ Elimination Cocktail (1000X) (Cat. No. G398).
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