Mycoplasma PCR Detection & Elimination Bundle
| Cat. No. | G238-G398 |
| Name | Mycoplasma PCR Detection & Elimination Bundle |
| Description |
Enjoy superior performance at lower costs! abm's Mycoplasma PCR Detection & Elimination Bundle combines the power of our bestselling kit Mycoplasma PCR Detection Kit (Cat. No. G238) and MycoAway™ Mycoplasma Elimination Cocktail (1000X) (Cat. No. G398). The Mycoplasma PCR Detection Kit offers highly specific and sensitive detection of 200+ strains of Mycoplasma in less than 2 hours. This bestselling kit is designed to minimize false positives, ensuring quick and reliable routine screening of cell cultures. A recent official publication by a national public health agency found that our kit out-performs Sigma and Lonza's kits while being 6X less expensive than ATCC's kit (Russell et al., 2020). Furthermore, the MycoAway™ Mycoplasma Elimination Cocktail (1000X) is a specially formulated agent to effectively clear cells from Mycoplasma contamination in both adherent and suspension cells. Our potent formulation is non-toxic and eliminates 70+ species of Mycoplasma in as little as 2-3 weeks (4-6 cell passages)! The combination of these two kits ensures complete protection of your cell lines! |
| Unit | Bundle |
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G238-G398 |
| What is the basis of the positive control in the mycoplasma kit? | |
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It is DNA fragments of Mycoplasma gDNA diluted in medium.
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| How much should I use for a T75 flask? | |
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For a T75 flask, we recommend using 5ml - 7ml of our Applied Cell Extracellular Matrix (G422).
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| What is the country of origin of TM999 FBS? | |
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Canadian Origin.
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| There is a ~200-250bp band in one of my samples, does this mean it is mycoplasma positive? | |
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In rare cases, there may be a weak non-specific binding to a region of human gDNA due to the degenerate nature of the primers that can amplify a weak 250bp band in human cell samples. Your sample can be considered as negative for mycoplasma, as mycoplasma amplicons will be 370-550bp in size. You can try to increase the annealing temperature of the PCR program to 60°C to reduce this non-specific amplification.
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| I did not see amplification of the positive control. What can I try? | |
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This could occur in cases where the PCR machine does not ramp temperature fast enough. We suggest trying touchdown PCR in these situations.
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| Can collected supernatant be stored at 4 degrees Celcius until we are ready to run the test? | |
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Yes, the supernatant can be stored at 4°C for a few weeks.
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| When collecting supernatant, there is a risk of pipetting cell debris. Can cell debris cause false positive bands? Is centrifugation required? | |
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Typically centrifugation is not required, however if your sample looks particularly cloudy, you can centrifuge it before use.
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| After running the PCR program, can the PCR reactions be left in the PCR machine overnight at 12 or 4 degrees Celsius? | |
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Yes it can be left in the PCR overnight at 12°C or 4°C.
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| Should PCR components be aliquoted to minimize multiple freeze/thaw cycles? | |
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For best results, PCR components should be aliquoted if you are frequently testing small quantites of samples. E.g. If you are only testing 1-5 samples each time, aliquoting is recommended to reduce the number of freeze/thaw cycles.
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| Will the serum in my samples inhibit the PCR reaction? | |
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Serum will not have any inhibitory effects on PCR. However, if your media contains >20% serum and if it is too viscous, you can dilute with either basal media or Nuclease-free water by tenfold prior to mycoplasma detection.
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| How do I use the Mycoplasma PCR Detection Kit in conjunction with the MycoAway™ Elimination Cocktail (1000X)? | |
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Mycoplasma (Cat. No. G238) or Mycoplasma Pro (Cat. No. G239) Detection Kits should be used first in order to identify contaminated cells. Either product will only require a small volume (2.5 μl) of culture supernatant. If cells are Mycoplasma-positive, treat cells with MycoAway™ Elimination Cocktail (1000X) (Cat. No. G398).
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