Mycoplasma PCR Detection Kit & Elimination Combo Kit
Cat. No.
G238-G398
Unit
1 Combo Kit
Price
$255.00
Specifications
| Cat. No. | G238-G398 |
| Name | Mycoplasma PCR Detection Kit & Elimination Combo Kit |
| Unit | 1 Combo Kit |
| Category | PCR, qPCR, and RT |
Documents
Supporting Protocol
FAQs
| What is the basis of the positive control in the mycoplasma kit? | |
|
It is DNA fragments of Mycoplasma gDNA diluted in medium.
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| There is a ~200-250bp band in one of my samples, does this mean it is mycoplasma positive? | |
|
In rare cases, there may be a weak non-specific binding to a region of human gDNA due to the degenerate nature of the primers that can amplify a weak 250bp band in human cell samples. Your sample can be considered as negative for mycoplasma, as mycoplasma amplicons will be 370-550bp in size. You can try to increase the annealing temperature of the PCR program to 60°C to reduce this non-specific amplification.
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